30 results about "Andrographolide" patented technology

Disclosed herein are synthetic nanoparticles, pharmaceutical compositions, kits, or methods for treating and / or preventing cancer. In some embodiments, the synthetic nanoparticles and / or pharmaceutical compositions comprises a pH sensitive liposome, an apolipoprotein, and andrographolide or derivative thereof. In some embodiments, the synthetic nanoparticles are delivered to a subject for treatment of cancer. In some embodiments, the cancer is T-cell lymphoma or B-cell lymphoma.

The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

The present application relates to the field of pharmacy, disclose the medicine composition for antibacterial and anti-inflammatory of Chinese herbal medicine and marine extract combination, including: baicalin extract 5~10 parts; andrographolide extract 3~7 parts; sulfated fucoidan 2~5 parts; spongin extract 0.2~1 part; wherein: baicalin extract is derived from the root of scrophulariaceae, is extracted by ethanol-water mixed solvent; andrographolide extract is derived from the whole plant of andrographis, is extracted by ethanol-water mixed solvent; sulfated fucoidan is derived from brown algae, is prepared by enzymolysis and alcohol precipitation method; spongin extract is derived from sponges, is extracted and separated and purified by organic solvent. Through the technical scheme of the synergistic combination of active ingredients of Chinese herbal medicine and marine extract, the technical effect of destroying the biofilm barrier and clearing the stubborn infection focus in the treatment of bacterial vaginitis, candida vaginitis and cervical inflammation associated with biofilm infection is achieved.

The invention relates to the technical field of medicines, and particularly discloses mupirocin ointment containing andrographolide and hydroxysafflor yellow A and a preparation method of the mupirocin ointment. The ointment comprises active ingredients of mupirocin, andrographolide and hydroxysafflor yellow A, and auxiliary materials of polyethylene glycol 3350 and polyethylene glycol 400. The mupirocin, the andrographolide and the hydroxysafflor yellow A are scientifically compounded, and different antibacterial mechanisms of the mupirocin, the andrographolide and the hydroxysafflor yellow A are utilized, so that a remarkable synergistic antibacterial effect is generated, drug-resistant bacteria can be effectively resisted, and generation of bacterial drug resistance is delayed. Meanwhile, by adding andrographolide and hydroxysafflor yellow A, the chemical stability of mupirocin in a polyethylene glycol matrix is accidentally improved, the validity period of the product is prolonged, and local microcirculation is improved. The invention further provides a preparation method of the ointment, the process is simple, and industrial production is easy.

The present invention provides methods for treating cancer in a subject, comprising administering to the subject an effective amount of a combination of andrographolide and melatonin. The present invention further provides compositions comprising effective amounts of andrographolide and melatonin for treating cancer.

The invention relates to the technical field of gene engineering detection, and particularly discloses a gene detection system for promoting andrographolide synthesis based on a biosensor, a key gene ApDof29 for regulating and controlling andrographolide synthesis is determined through whole genome analysis, gene expression profile research and co-expression network analysis, the core regulation and control effect of ApDof29 is verified, and the gene detection system for promoting andrographolide synthesis based on the biosensor is used for promoting andrographolide synthesis. The invention also develops a real-time dynamic monitoring system, and realizes non-invasive monitoring of andrographolide content accumulation, biosynthesis rate and metabolic intermediate concentration change by utilizing the fusion of a specific response element and a reporter gene. Through comprehensive calculation processing of an accumulation abnormal coefficient, a synthesis rate abnormal coefficient and a concentration change abnormal coefficient, the regulation and control effect of ApDof29 is dynamically evaluated, and a scientific basis is provided for subsequent gene modification.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine formula for treating haemorrhoids and a preparation method thereof. The formula comprises ephedra, semen brassicae, cinnamon, rhizoma zingiberis, antler slices, bombyx batryticatus, smoked plum, catechu, golden cypress, coix seeds, peach kernels, snakegourd seeds, sanguisorba officinalis, hairyvein agrimony, radix angelicae, purslane, red halloysite, dragon's blood, gallnut, andrographolide extract and carboxymethyl pachymaran, and preferably comprises sophora flowers and fructus aurantii. The preparation method comprises the following steps: firstly, carrying out physical field auxiliary activation on animal and mineral medicinal materials in the formula, carrying out synergistic aging on high-tannin medicinal materials, and carrying out co-crushing pretreatment on sophora flowers and fructus aurantii; carrying out alternating magnetic field assisted dynamic countercurrent extraction on the pretreated medicinal materials; carrying out membrane separation and refining on an extracting solution, and carrying out oriented assembly on the refined extracting solution, an andrographolide extract and carboxymethyl pachymaran; and finally carrying out programmed molding according to the requirements of oral solid preparations or decoctions. The composition can be used for treating haemorrhoids, and has the advantages that multiple components have a synergistic effect, and related symptoms of the haemorrhoids can be conditioned in a targeted manner.

The application discloses a hydroxylase and application thereof in synthesis of 14-deoxoandrographolide. The hydroxylase is protein CYP72A399, and the amino acid sequence of the protein CYP72A399 is shown as SEQ ID No: 2. Experiments prove that the protein CYP72A399 can be used as a hydroxylase to catalyze new andrographolide aglycone to generate 14-deoxoandrographolide. Therefore, the protein CYP72A399 has important theoretical and practical significance for synthesis of 14-deoxoandrographolide and cultivation of high-quality andrographis paniculata. The application has important application value.

The present invention relates to a cosmetic composition comprising andrographolide and an extract of Bidens pilosa L. as active ingredients. The cosmetic composition of the present invention exhibits excellent effects superior to those of when each of andrographolide or an extract of Bidens pilosa L. is used alone on wrinkle alleviation, skin irritation alleviation, elasticity improvement, inflammation, and antioxidation by using andrographolide and the extract of Bidens pilosa L. together.

The invention discloses an anti-inflammatory and anti-aging face cream and a preparation method thereof, and belongs to the technical field of cosmetics. The face cream disclosed by the invention is prepared from the following components in percentage by weight: 5.0 to 20.0 percent of broccoli fermentation filtrate, 0.05 to 0.2 percent of andrographolide encapsulated by nano-liposome, 0.5 to 5.0 percent of a skin barrier repairing agent and a matrix. Sulforaphane (greater than or equal to 400 mg / kg) in broccoli is enriched through a lactic acid bacteria fermentation technology, and the stability and the smell of the broccoli are improved; the problem that andrographolide is difficult to dissolve and easy to crystallize is solved through embedding of nano-liposome (the particle size is 40-80 nm, and the encapsulation efficiency is larger than or equal to 85%), and the transdermal property of andrographolide is improved. The two are combined to synergistically activate an Nrf2 antioxidant pathway and inhibit an NF-kappa B inflammation pathway, and the two pathways intervene in inflammatory aging. Human body tests show that after the face cream is used for 4 weeks, the depth of wrinkles can be remarkably reduced (28.6%), the level of skin inflammatory factors TNF-alpha can be reduced (50.4%), and the face cream is free of any irritating reaction, has an excellent anti-aging effect and extremely high safety and is particularly suitable for sensitive skin.

The invention discloses an andrographis paniculata transcription factor ApMYB9 and an application of the andrographis paniculata transcription factor ApMYB9 in positive regulation of biosynthesis of andrographolide. The key regulatory factor ApMYB9 for andrographolide synthesis is explored, the molecular mechanism of the key regulatory factor ApMYB9 for positively regulating and controlling andrographolide synthesis through an important enzyme gene isopentene diphosphate isomerase ApIPPI of a targeted andrographolide synthesis pathway is verified, and the application of the key regulatory factor ApMYB9 for improving the content of andrographolide by utilizing a biotechnology is provided. And a theoretical basis is provided for culturing a new variety of andrographis paniculata with high diterpene lactone content.

The application discloses application of triacetylandrographolide and a composition thereof in preparation of a medicine for relaxing airway smooth muscle, and belongs to the technical field of medicines. In view of the problems of obvious side effects and narrow treatment window of existing medicines, the application finds that triacetylandrographolide (3, 14, 19-Triacetylandrographolide, TAA) and the composition thereof have tracheal relaxation activity, and the triacetylandrographolide and the composition thereof play a relaxation role through non-competitive antagonism of a downstream signal path of a cholinergic receptor. The new mechanism provides a new idea for developing a new bronchial dilator for overcoming drug resistance of existing medicines. The TAA after preparation optimization can be used for treating or preventing airway obstructive diseases such as asthma, chronic obstructive pulmonary disease, bronchitis and acute lung injury.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

Described herein are compositions (e.g., topical formulations) including andrographolides (ANGL), epigallocatechin gallate (EGCG), or a combination thereof, and methods of making and using the same.

The invention discloses an andrographolide-based pH / ROS (Potential of Hydrogen / Reactive Oxygen Species) double-response double-drug loaded nano material as well as preparation and application thereof. The material is composed of an amphiphilic polymer (PEG-fan-shaped lysine-formyl phenylboronic acid) and hydrophobic drugs A and B, the drug A is covalently bound with the polymer through a borate bond to form a prodrug, and the drug B is physically wrapped in a nanoparticle hydrophobic core. The acidity and high reactive oxygen species (ROS) microenvironment of inflammatory lesions are utilized to trigger breakage of a Schiff base bond and a borate bond in a molecular chain, and the nanoparticles are induced to be disassembled and synergistically release double drugs. According to the invention, the enrichment degree and bioavailability of the drug at the lung inflammation part are obviously improved through passive targeting, efficient inhibition of inflammatory factor storm is realized, and a new scheme is provided for accurate delivery of hydrophobic anti-inflammatory drugs.

The invention discloses a pharmaceutical composition for targeted removal of osteoarthritis articular cavity apoptotic cells and application, and belongs to the technical field of biological medicines. The pharmaceutical composition comprises a targeting ligand, an apoptotic cell scavenger and a drug carrier, the targeting ligand is selected from an anti-phosphatidylserine monoclonal antibody or Annexin V protein, and can be specifically combined with phosphatidylserine on the surface of an apoptotic cell; the apoptotic cell scavenger is selected from demethoxycurcumin, andrographolide and the like, and can promote apoptotic cell phagocytosis and inhibit inflammatory factor release; and the drug carrier is hyaluronic acid-polylactic acid copolymer nanoparticles, and has good articular cavity retention property and drug sustained release effect. The pharmaceutical composition disclosed by the invention can be used for accurately targeting apoptotic cells in osteoarthritis articular cavities, efficiently removing the apoptotic cells, inhibiting inflammatory response and delaying cartilage degeneration, is good in biological safety and provides a new effective means for treating osteoarthritis.

The invention discloses an isopentene diphosphate isomerase ApIPPI and an application of the isopentene diphosphate isomerase ApIPPI. According to the present invention, the isopentene diphosphate isomerase ApIPPI is found, and the experimental verification results show that the synthesis amount of andrographolide can be effectively improved by overexpressing the gene of the isopentene diphosphate isomerase ApIPPI in andrographis paniculata, such that the theoretical basis is provided for the improvement of the andrographolide content and the cultivation of the new andrographolide variety with the high diterpene lactone content by using the biotechnology.

The invention provides application of andrographolide in promoting calf growth and relieving weaning stress based on a microbial community regulation effect, and belongs to the technical field of animal feeding. The andrographolide (AG) can significantly improve the growth performance and the health level of the calves, relieve the weaning stress of the calves and improve the respiratory tract health of the calves by inhibiting inflammation and oxidative stress and systematically regulating and controlling microbial communities at multiple parts. Under the low-dosage feeding level, AG is more prone to optimizing rumen microflora, rumen is promoted to turn to an efficient propionic acid type fermentation mode, and sustainable benefits are achieved; and under a high dosage level, AG exerts a stronger anti-inflammatory and anti-oxidation effect to realize short-term benefit maximization. AG is added, so that the cross-site interaction between microbial communities in the digestive tract and the nasal cavity is enhanced, the colonization of various potential pathogens in the nasal cavity is remarkably inhibited, and the respiratory tract health of the calf is improved.

The invention discloses application of andrographolide and a pharmaceutical composition thereof in preparation of a medicine for preventing and / or treating lung cancer, particularly lung metastatic tumor, and belongs to the technical field of medicine. In-vivo and in-vitro experiments of a system prove that andrographolide can inhibit activation of a nuclear factor kappa B (NF-kappa B) signal channel by down-regulating expression of glycogen synthase kinase 3beta (GSK3beta), and particularly, phosphorylation and nuclear translocation processes of an NF-kappa B p65 subunit Ser536 site are inhibited. According to the effect, transcription and expression of downstream target gene chemotactic factors CCL2 and CXCL1 are effectively reduced, finally, infiltration of tumor-related macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs) in tumor tissue is remarkably reduced, aggregation of anti-tumor immune cells such as CD8 + T cells is promoted, an immunosuppressive microenvironment is reversed into an immune activation state, and the immunosuppressive activity of tumor cells is improved. And lung cancer metastasis is inhibited from the source. The invention provides a brand new candidate drug and a clear action mechanism for clinical prevention and treatment of lung cancer metastasis, and has a good development prospect.

The invention discloses a method for rapidly evaluating the quality of an andrographis paniculata medicinal material, which adopts a near infrared spectrum technology, can be used for determination after a sample is crushed, does not need complex pretreatment operation on the sample, is simple, convenient and rapid, only needs several minutes for detection, does not need chemical reagents for dissolution, and can be used for rapidly evaluating the quality of the andrographis paniculata medicinal material. The contents of chlorogenic acid, andrographolide and dehydroandrographolide can be measured at the same time, and the content of limited components in the andrographis paniculata medicinal material is comprehensively evaluated.

This invention belongs to the field of biomedicine and discloses the application of natural isoandrographolide in anti-stress and anti-aging. Using a Caenorhabditis elegans model, this invention discovered that isoandrographolide possesses anti-stress and anti-aging effects. In vivo experiments demonstrated that isoandrographolide exhibits anti-stress and anti-aging activities, with advantages such as safety, non-toxicity, and high bioactivity. It also significantly improves the healthy lifespan indicators of nematodes, maintaining better motility than the control group at different time points in adulthood, while increasing survival rate under heat stress conditions. Furthermore, this lifespan extension does not come at the cost of reproductive damage or food intake restriction. Mechanistic studies revealed that the anti-stress and anti-aging activities of isoandrographolide are related to the downregulation of genes fat-5 and fat-6. Therefore, it can be used as a functional ingredient in the development of anti-stress or anti-aging foods, health products, cosmetics, and pharmaceuticals, showing good potential and application prospects.

The present invention provides substituted 14-deoxy-11,12-didehydroandrographolide (14- DDA) compounds. The present invention particularly provides substituted 14-deoxy-11,12- didehydroandrographolide (14-DDA) compounds having substitutions formed to the exocyclic C8-17 double bond. The present invention also provides their method of preparation, pharmaceutical compositions thereof and their use as anti-viral agents. In comparison the parent molecule andrographolide (<90% viral reduction), B1 and B8 show more than 90% viral reduction at all three concentrations in the preliminary screening, they were taken for further studies to determine their EC50. Upon analysis, the EC50 for B1 and B8 were obtained 0.329µM and 0.153µM respectively.

The application provides a method for improving androstane content based on methyl jasmonate and ApMYC2-ApHSFB2b module, and belongs to the field of plant biotechnology. The application constructs an overexpression vector of ApMYC2 gene and an overexpression vector of ApHSFB2b gene respectively, and transforms the two overexpression vectors into andrographis plants; after transformation, the andrographis plants are treated with methyl jasmonate again. Through the transformation of the overexpression vectors and the treatment of methyl jasmonate, the two work together to directly activate the expression of ApCPS1, a gene in the andrographolide biosynthesis pathway, and then promote the expression of other genes downstream of the synthesis pathway, so as to improve the androstane content, and provide an effective technical means for the planting and development of andrographis.

This invention provides a CYP450 enzyme protein, its encoding gene, and its applications that can catalyze the formation of new andrographolide aglycones, belonging to the field of biology. This invention utilizes bioinformatics analysis and differential expression analysis of transcriptome data from different tissue sites to identify the gene ApCYP71BE50, which catalyzes the formation of new andrographolide aglycones from (4R,5S,9R,10S)-labda-8(17),13-dien-15,19-diol. It also verifies that the CYP450 enzyme protein encoded by the ApCYP71BE50 gene can continuously oxidize the C16 position of (4R,5S,9R,10S)-labda-8(17),13-dien-15,19-diol to form new andrographolide aglycones. This invention provides an important gene element for the biosynthesis of andrographolide and also provides a key gene locus for the molecular design breeding of andrographis paniculata.

A process for the manufacture of beverages having a hop-like bitterness or for the hop-like bittering of beverages. The process includes adding to a beverage base at least one of: andrographolide, foliamenthin, menthiafolin, dihydrofoliamenthin, including derivatives thereof, at a concentration of from about 0.1 ppm to about 50 ppm. Use of at least one of andrographolide, foliamenthin, menthiafolin, or dihydrofoliamenthin as a hop-like bittering agent in a beverage. A method for replacing or reducing the amount of hop-derived ingredients in a beverage without the concomitant reduction in hop-like bitterness including adding at least one of andrographolide, foliamenthin, menthiafolin, or dihydrofoliamenthin to a beverage base at a concentration of from about 0.1 ppm to about 50 ppm.

1. The name of the design product: packaging box (compound Andrographis herb tablet). 2. The use of the design product: the design product is used for packaging food, medicine or health food. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: design 2 unfolded state diagram. 5. Design 2 is designated as the basic design. 6. Other circumstances that need to be explained: the color of design 1 is requested.

The invention relates to the technical field of biological medicines, and particularly discloses a preparation method and application of an exosome composition for multi-target treatment of pulmonary nodules, the exosome composition is composed of a targeted drug-loaded exosome, a grape seed exosome and a tissue ecological restoration factor; wherein the targeted drug-loaded exosome is modified by cRGD peptide to realize active targeting, and is loaded with andrographolide and rosmarinic acid at the same time; the grape seed exosome is extracted from grape seeds and has the functions of regulating immune microenvironment and inhibiting inflammation; the tissue ecological restoration factor is prepared from N-acetylcysteine, nicotinamide and epigallocatechin gallate; the composition realizes the organic combination of precise targeted drug delivery, immune microenvironment regulation and tissue ecological restoration through a multi-component and multi-target synergistic effect, and shows a remarkable pulmonary nodule inhibition effect and good safety in in-vivo and in-vitro experiments.

The invention relates to a whitening and freckle-removing composition and whitening cream, and belongs to the technical field of cosmetics, the whitening and freckle-removing composition comprises the following raw materials: a black mulberry fruit extract, a snow lotus herb extract, nicotinamide, andrographolide, and diacetylboldine; the whitening cream comprises the whitening and freckle-removing composition and further comprises auxiliary components such as a skin conditioner, an emollient and an emulsifier. The black mulberry fruit extract, the saussurea involucrata extract and the nicotinamide are combined for use, so that the prepared finished product has a whitening effect by virtue of a multi-dimension synergistic effect of blocking melanin synthesis, inhibiting pigmentation, accelerating melanin excretion and reducing uranidin; the andrographolide, the diacetylboldine and the saussurea involucrata extract are combined, the andrographolide is used for reducing color spot inducing factors, the diacetylboldine is used for resisting hyperpigmentation, the saussurea involucrata extract is used for promoting skin blood circulation, and all the components supplement one another, so that the prepared finished product further has the effect of fading color spots.

The present application relates to the technical field of biological medicine, and particularly relates to a composition taking GLP-1 receptor as a target point, which comprises the following raw materials in parts by weight: gentiana scabra extract 10-25 parts, andrographolide sulfonate 5-15 parts, peganum harmala alkaloid 3-7 parts, curcumin functional compound 20-40 parts, and excipients; the excipients comprise sodium caprate 4-8 parts and konjac glucomannan 8-15 parts. The composition of the present application realizes the ordered synergistic effect in time and space through the triple mechanism of "promoting endogenous GLP-1 secretion at the intestinal end, directly activating GLP-1R at the receptor end and prolonging the half-life, and promoting beta cell regeneration and protection at the effector organ end", reshapes the steady-state regulation ability of the body's own GLP-1 signal axis, improves sugar metabolism, and is suitable for the prevention and treatment of diabetes, obesity and related metabolic diseases.