47 results about "Andrographolide" patented technology

Disclosed herein are synthetic nanoparticles, pharmaceutical compositions, kits, or methods for treating and / or preventing cancer. In some embodiments, the synthetic nanoparticles and / or pharmaceutical compositions comprises a pH sensitive liposome, an apolipoprotein, and andrographolide or derivative thereof. In some embodiments, the synthetic nanoparticles are delivered to a subject for treatment of cancer. In some embodiments, the cancer is T-cell lymphoma or B-cell lymphoma.

The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

The present application relates to the field of pharmacy, disclose the medicine composition for antibacterial and anti-inflammatory of Chinese herbal medicine and marine extract combination, including: baicalin extract 5~10 parts; andrographolide extract 3~7 parts; sulfated fucoidan 2~5 parts; spongin extract 0.2~1 part; wherein: baicalin extract is derived from the root of scrophulariaceae, is extracted by ethanol-water mixed solvent; andrographolide extract is derived from the whole plant of andrographis, is extracted by ethanol-water mixed solvent; sulfated fucoidan is derived from brown algae, is prepared by enzymolysis and alcohol precipitation method; spongin extract is derived from sponges, is extracted and separated and purified by organic solvent. Through the technical scheme of the synergistic combination of active ingredients of Chinese herbal medicine and marine extract, the technical effect of destroying the biofilm barrier and clearing the stubborn infection focus in the treatment of bacterial vaginitis, candida vaginitis and cervical inflammation associated with biofilm infection is achieved.

The triacetyl andrographolide nanocrystal is mainly composed of triacetyl andrographolide, a stabilizer and an excipient, and an average particle size of drug particles in a triacetyl andrographolide nanocrystal suspension obtained by redissolving the triacetyl andrographolide nanocrystal in water, is less than 500 nm, and a PDI is less than 0.2. The nanocrystal suspension is prepared from the triacetyl andrographolide and the stabilizer by a high-speed shear anti-solvent method in combination with a high-pressure homogenization method, then the excipient is added, and the nanocrystal is prepared through spray-drying.

The invention relates to the technical field of medicines, and particularly discloses mupirocin ointment containing andrographolide and hydroxysafflor yellow A and a preparation method of the mupirocin ointment. The ointment comprises active ingredients of mupirocin, andrographolide and hydroxysafflor yellow A, and auxiliary materials of polyethylene glycol 3350 and polyethylene glycol 400. The mupirocin, the andrographolide and the hydroxysafflor yellow A are scientifically compounded, and different antibacterial mechanisms of the mupirocin, the andrographolide and the hydroxysafflor yellow A are utilized, so that a remarkable synergistic antibacterial effect is generated, drug-resistant bacteria can be effectively resisted, and generation of bacterial drug resistance is delayed. Meanwhile, by adding andrographolide and hydroxysafflor yellow A, the chemical stability of mupirocin in a polyethylene glycol matrix is accidentally improved, the validity period of the product is prolonged, and local microcirculation is improved. The invention further provides a preparation method of the ointment, the process is simple, and industrial production is easy.

This invention proposes a soothing andrographolide, its preparation method, and its applications, belonging to the field of cosmetic technology. The method involves sterilizing and inducing culture of *Andrographis paniculata* leaves, followed by crushing, enzymatic hydrolysis, and extraction with a deep eutectic solvent to obtain the extract. This extract is then added to an adsorbent, stirred for adsorption, filtered, desorbed, and reacted with salicylic acid to obtain andrographolide salicylate ester. Alcoholic esters are then prepared, thus yielding the soothing andrographolide. The soothing andrographolide prepared by this invention significantly improves the product's water solubility and bioavailability, is safe and non-irritating to the skin, and has anti-redness, anti-allergic, anti-inflammatory, and soothing effects. The addition of alcohol improves the flexibility and extensibility of the structure, resulting in better skin permeability than liposomes and stronger transdermal penetration, thereby expanding its application scope.

The present invention provides methods for treating cancer in a subject, comprising administering to the subject an effective amount of a combination of andrographolide and melatonin. The present invention further provides compositions comprising effective amounts of andrographolide and melatonin for treating cancer.

The invention relates to the technical field of gene engineering detection, and particularly discloses a gene detection system for promoting andrographolide synthesis based on a biosensor, a key gene ApDof29 for regulating and controlling andrographolide synthesis is determined through whole genome analysis, gene expression profile research and co-expression network analysis, the core regulation and control effect of ApDof29 is verified, and the gene detection system for promoting andrographolide synthesis based on the biosensor is used for promoting andrographolide synthesis. The invention also develops a real-time dynamic monitoring system, and realizes non-invasive monitoring of andrographolide content accumulation, biosynthesis rate and metabolic intermediate concentration change by utilizing the fusion of a specific response element and a reporter gene. Through comprehensive calculation processing of an accumulation abnormal coefficient, a synthesis rate abnormal coefficient and a concentration change abnormal coefficient, the regulation and control effect of ApDof29 is dynamically evaluated, and a scientific basis is provided for subsequent gene modification.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine formula for treating haemorrhoids and a preparation method thereof. The formula comprises ephedra, semen brassicae, cinnamon, rhizoma zingiberis, antler slices, bombyx batryticatus, smoked plum, catechu, golden cypress, coix seeds, peach kernels, snakegourd seeds, sanguisorba officinalis, hairyvein agrimony, radix angelicae, purslane, red halloysite, dragon's blood, gallnut, andrographolide extract and carboxymethyl pachymaran, and preferably comprises sophora flowers and fructus aurantii. The preparation method comprises the following steps: firstly, carrying out physical field auxiliary activation on animal and mineral medicinal materials in the formula, carrying out synergistic aging on high-tannin medicinal materials, and carrying out co-crushing pretreatment on sophora flowers and fructus aurantii; carrying out alternating magnetic field assisted dynamic countercurrent extraction on the pretreated medicinal materials; carrying out membrane separation and refining on an extracting solution, and carrying out oriented assembly on the refined extracting solution, an andrographolide extract and carboxymethyl pachymaran; and finally carrying out programmed molding according to the requirements of oral solid preparations or decoctions. The composition can be used for treating haemorrhoids, and has the advantages that multiple components have a synergistic effect, and related symptoms of the haemorrhoids can be conditioned in a targeted manner.

The application discloses a hydroxylase and application thereof in synthesis of 14-deoxoandrographolide. The hydroxylase is protein CYP72A399, and the amino acid sequence of the protein CYP72A399 is shown as SEQ ID No: 2. Experiments prove that the protein CYP72A399 can be used as a hydroxylase to catalyze new andrographolide aglycone to generate 14-deoxoandrographolide. Therefore, the protein CYP72A399 has important theoretical and practical significance for synthesis of 14-deoxoandrographolide and cultivation of high-quality andrographis paniculata. The application has important application value.

The invention belongs to the technical field of stem cells, and particularly relates to an in-vitro amplification process of umbilical cord mesenchymal stem cells. The in-vitro amplification process of the umbilical cord mesenchymal stem cells comprises the following steps: inoculating the mesenchymal stem cells into a DMEM-containing low-sugar culture medium, adding noricaritin, a recombinant human epidermal growth factor and andrographolide, continuously culturing, and collecting the cells. According to the method, low-dose andrographolide, noricaritin and epidermal growth factors are added into a basic culture medium in stages, synergistic interaction is achieved among the andrographolide, the noricaritin and the epidermal growth factors, cell proliferation is promoted, cell activity is improved, meanwhile, the synergistic pharmacological action is achieved, the active oxygen level is reduced, oxidative damage is inhibited, and a continuously-amplified low-oxidation environment is created for cells; the cell dryness and the long-term passage capability are maintained.

The present invention relates to a cosmetic composition comprising andrographolide and an extract of Bidens pilosa L. as active ingredients. The cosmetic composition of the present invention exhibits excellent effects superior to those of when each of andrographolide or an extract of Bidens pilosa L. is used alone on wrinkle alleviation, skin irritation alleviation, elasticity improvement, inflammation, and antioxidation by using andrographolide and the extract of Bidens pilosa L. together.

The invention discloses an anti-inflammatory and anti-aging face cream and a preparation method thereof, and belongs to the technical field of cosmetics. The face cream disclosed by the invention is prepared from the following components in percentage by weight: 5.0 to 20.0 percent of broccoli fermentation filtrate, 0.05 to 0.2 percent of andrographolide encapsulated by nano-liposome, 0.5 to 5.0 percent of a skin barrier repairing agent and a matrix. Sulforaphane (greater than or equal to 400 mg / kg) in broccoli is enriched through a lactic acid bacteria fermentation technology, and the stability and the smell of the broccoli are improved; the problem that andrographolide is difficult to dissolve and easy to crystallize is solved through embedding of nano-liposome (the particle size is 40-80 nm, and the encapsulation efficiency is larger than or equal to 85%), and the transdermal property of andrographolide is improved. The two are combined to synergistically activate an Nrf2 antioxidant pathway and inhibit an NF-kappa B inflammation pathway, and the two pathways intervene in inflammatory aging. Human body tests show that after the face cream is used for 4 weeks, the depth of wrinkles can be remarkably reduced (28.6%), the level of skin inflammatory factors TNF-alpha can be reduced (50.4%), and the face cream is free of any irritating reaction, has an excellent anti-aging effect and extremely high safety and is particularly suitable for sensitive skin.

The invention discloses an andrographis paniculata transcription factor ApMYB9 and an application of the andrographis paniculata transcription factor ApMYB9 in positive regulation of biosynthesis of andrographolide. The key regulatory factor ApMYB9 for andrographolide synthesis is explored, the molecular mechanism of the key regulatory factor ApMYB9 for positively regulating and controlling andrographolide synthesis through an important enzyme gene isopentene diphosphate isomerase ApIPPI of a targeted andrographolide synthesis pathway is verified, and the application of the key regulatory factor ApMYB9 for improving the content of andrographolide by utilizing a biotechnology is provided. And a theoretical basis is provided for culturing a new variety of andrographis paniculata with high diterpene lactone content.

The application belongs to the technical field of medicine, and particularly relates to a andrographolide anhydrous swallowing granule and a preparation method thereof. The andrographolide anhydrous swallowing granule is formed by mixing drug-containing microcapsules and flavoring auxiliaries. The drug-containing microcapsules are prepared by centrifugal hot melt spraying technology from andrographolide and high-melting-point capsule materials. The high-melting-point capsule materials are one or more of Brazil palm wax, hydrogenated castor oil, microcrystalline wax, rice bran wax and sugarcane wax. The flavoring auxiliaries are fillers, sweeteners, essences and pH regulators. The prepared granule has the characteristics of small particle size, smooth surface, good fluidity, no grit feeling, no sticky teeth feeling and easy swallowing. Moreover, the granule has a significant taste masking effect, no bitterness, moderate sweetness and moderate fragrance, which helps to improve patient compliance, and is particularly suitable for old people and children patients who have difficulty in swallowing. The centrifugal hot melt spraying technology adopted by the application is simple in process, low in cost, green and pollution-free, and suitable for large-scale production.

The invention relates to the technical field of inhalation preparations, in particular to an inhalation preparation for relieving or treating upper respiratory tract infection and a preparation method and application thereof. The inhalation preparation comprises an active pharmaceutical ingredient, and the active pharmaceutical ingredient is andrographolide; the inhalation preparation also comprises pharmaceutically acceptable auxiliary materials; the pharmaceutically acceptable auxiliary materials comprise one or more of a solvent, a cosolvent, a propellant, a carrier / diluent, a pH regulator, an antistatic agent and a stabilizer. The prescriptions and the preparation methods of the aerosol, the dry powder inhalation and the spray are suitable for industrial production, and clinical tests prove that the prepared inhalation preparation has a remarkable relieving or treating effect on upper respiratory tract infection.

The utility model relates to the technical field of andrographolide cleaning, and discloses an andrographolide purifying device which comprises a cleaning box and a transverse plate, a flexible net belt is sleeved on an outer ring between rotating rollers on two sides in a transmission mode, and flexible side nets are fixed on outer rings of the front end and the rear end of the flexible net belt in a surrounding mode. A plurality of separation nets are evenly and longitudinally fixed between the front-end flexible side net and the rear-end flexible side net, a spraying pipe is longitudinally fixed in the cleaning box above the flexible net belt, and a plurality of electric heating nets are evenly and longitudinally fixed between the front-end vertical plate and the rear-end vertical plate. According to the technical scheme, the flexible net belt provided with the flexible side net and the separation net is driven to rotate inside and above the cleaning box, andrographolide can be placed on the surface of the flexible net belt between the separation net and the flexible side net to be conveyed, the andrographolide is cleaned through clean water sprayed by the spray heads of the spray pipe, and the cleaning efficiency is improved. The andrographolide is conveyed to the upper portion and then heated and dried by the electric heating net, waste heat generated after drying can heat clean water, it is guaranteed that andrographolide is efficiently and cleanly cleaned, and then direct storage is facilitated.

The application discloses application of triacetylandrographolide and a composition thereof in preparation of a medicine for relaxing airway smooth muscle, and belongs to the technical field of medicines. In view of the problems of obvious side effects and narrow treatment window of existing medicines, the application finds that triacetylandrographolide (3, 14, 19-Triacetylandrographolide, TAA) and the composition thereof have tracheal relaxation activity, and the triacetylandrographolide and the composition thereof play a relaxation role through non-competitive antagonism of a downstream signal path of a cholinergic receptor. The new mechanism provides a new idea for developing a new bronchial dilator for overcoming drug resistance of existing medicines. The TAA after preparation optimization can be used for treating or preventing airway obstructive diseases such as asthma, chronic obstructive pulmonary disease, bronchitis and acute lung injury.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

Described herein are compositions (e.g., topical formulations) including andrographolides (ANGL), epigallocatechin gallate (EGCG), or a combination thereof, and methods of making and using the same.

The invention discloses an andrographolide-based pH / ROS (Potential of Hydrogen / Reactive Oxygen Species) double-response double-drug loaded nano material as well as preparation and application thereof. The material is composed of an amphiphilic polymer (PEG-fan-shaped lysine-formyl phenylboronic acid) and hydrophobic drugs A and B, the drug A is covalently bound with the polymer through a borate bond to form a prodrug, and the drug B is physically wrapped in a nanoparticle hydrophobic core. The acidity and high reactive oxygen species (ROS) microenvironment of inflammatory lesions are utilized to trigger breakage of a Schiff base bond and a borate bond in a molecular chain, and the nanoparticles are induced to be disassembled and synergistically release double drugs. According to the invention, the enrichment degree and bioavailability of the drug at the lung inflammation part are obviously improved through passive targeting, efficient inhibition of inflammatory factor storm is realized, and a new scheme is provided for accurate delivery of hydrophobic anti-inflammatory drugs.

The invention discloses a pharmaceutical composition for targeted removal of osteoarthritis articular cavity apoptotic cells and application, and belongs to the technical field of biological medicines. The pharmaceutical composition comprises a targeting ligand, an apoptotic cell scavenger and a drug carrier, the targeting ligand is selected from an anti-phosphatidylserine monoclonal antibody or Annexin V protein, and can be specifically combined with phosphatidylserine on the surface of an apoptotic cell; the apoptotic cell scavenger is selected from demethoxycurcumin, andrographolide and the like, and can promote apoptotic cell phagocytosis and inhibit inflammatory factor release; and the drug carrier is hyaluronic acid-polylactic acid copolymer nanoparticles, and has good articular cavity retention property and drug sustained release effect. The pharmaceutical composition disclosed by the invention can be used for accurately targeting apoptotic cells in osteoarthritis articular cavities, efficiently removing the apoptotic cells, inhibiting inflammatory response and delaying cartilage degeneration, is good in biological safety and provides a new effective means for treating osteoarthritis.

The invention relates to the technical field of pharmaceutical compositions, in particular to a pharmaceutical composition for synergistically inhibiting klebsiella pneumoniae and infectious pneumonia of the klebsiella pneumoniae, a preparation and application, and the composition is prepared from the following components in parts by weight: 50-200 parts of berberine, 10-100 parts of andrographolide, 10-100 parts of manuka honey and 10-50 parts of polymyxin B. In-vitro antibacterial study is carried out on the berberine, the andrographolide, the manuka honey and the polymyxin B, and it is found that the andrographolide, the berberine and the manuka honey can all improve the antibacterial effect of the polymyxin B and all generate a synergistic effect when being combined for use; besides, the combination of the four medicines provided by the invention is used for treating a mouse suffering from Klebsiella pneumonia infectious severe pneumonia, the effect of the combination of the four medicines is obviously superior to that of a single medicine, and the effect of treating the Klebsiella pneumonia infectious severe pneumonia can be obviously improved after the medicine composition provided by the invention is compounded.

The invention discloses an isopentene diphosphate isomerase ApIPPI and an application of the isopentene diphosphate isomerase ApIPPI. According to the present invention, the isopentene diphosphate isomerase ApIPPI is found, and the experimental verification results show that the synthesis amount of andrographolide can be effectively improved by overexpressing the gene of the isopentene diphosphate isomerase ApIPPI in andrographis paniculata, such that the theoretical basis is provided for the improvement of the andrographolide content and the cultivation of the new andrographolide variety with the high diterpene lactone content by using the biotechnology.

The present invention provides a drug for treating non-small cell lung cancer, belonging to the field of medicine. The drug's raw materials include gefitinib and andrographolide, wherein the concentration of gefitinib is 12-20 μM and the concentration of andrographolide is 200-250 μM. The drug is in the form of an oral liquid preparation or an oral tablet. Experimental results show that the drug provided by the present invention has a significant inhibitory effect on the expression of apoptosis-related proteins and genes in PC9 / G cells, with a greater inhibitory effect than gefitinib or andrographolide alone, indicating a certain synergistic relationship between the two.

The application discloses a group of andrographolide synthesis related P450 cytochrome enzymes and application thereof, and belongs to the field of bioengineering. On the basis of original sequences CYP71A8 and CYP71D10 from Andrographis Herba, the signal peptide sequence is truncated, and the heterologous expression of CYP7A8 and CYP71D10 after the signal peptide is successfully realized. In the application, the host is Saccharomyces cerevisiae CEN.PK2-1D, the ROX1 and GAL80 genes on the genome are knocked out, and the coding genes of GGPP synthase and CPS diterpene synthase are integrated at the ROX1 site; CYP71A8 and CYP71D10 and ApCPR are expressed freely, and the recombinant Saccharomyces cerevisiae is successfully constructed, the de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid is realized, and the response value of the product peak reaches 1.9*106 compared with the blank, which is the only related report on the biosynthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid at present. The strategy provides a necessary reference for analyzing the andrographolide biosynthesis pathway and synthesizing andrographolide and related derivatives by metabolic engineering.

The invention provides application of andrographolide in promoting calf growth and relieving weaning stress based on a microbial community regulation effect, and belongs to the technical field of animal feeding. The andrographolide (AG) can significantly improve the growth performance and the health level of the calves, relieve the weaning stress of the calves and improve the respiratory tract health of the calves by inhibiting inflammation and oxidative stress and systematically regulating and controlling microbial communities at multiple parts. Under the low-dosage feeding level, AG is more prone to optimizing rumen microflora, rumen is promoted to turn to an efficient propionic acid type fermentation mode, and sustainable benefits are achieved; and under a high dosage level, AG exerts a stronger anti-inflammatory and anti-oxidation effect to realize short-term benefit maximization. AG is added, so that the cross-site interaction between microbial communities in the digestive tract and the nasal cavity is enhanced, the colonization of various potential pathogens in the nasal cavity is remarkably inhibited, and the respiratory tract health of the calf is improved.

The invention discloses application of andrographolide and a pharmaceutical composition thereof in preparation of a medicine for preventing and / or treating lung cancer, particularly lung metastatic tumor, and belongs to the technical field of medicine. In-vivo and in-vitro experiments of a system prove that andrographolide can inhibit activation of a nuclear factor kappa B (NF-kappa B) signal channel by down-regulating expression of glycogen synthase kinase 3beta (GSK3beta), and particularly, phosphorylation and nuclear translocation processes of an NF-kappa B p65 subunit Ser536 site are inhibited. According to the effect, transcription and expression of downstream target gene chemotactic factors CCL2 and CXCL1 are effectively reduced, finally, infiltration of tumor-related macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs) in tumor tissue is remarkably reduced, aggregation of anti-tumor immune cells such as CD8 + T cells is promoted, an immunosuppressive microenvironment is reversed into an immune activation state, and the immunosuppressive activity of tumor cells is improved. And lung cancer metastasis is inhibited from the source. The invention provides a brand new candidate drug and a clear action mechanism for clinical prevention and treatment of lung cancer metastasis, and has a good development prospect.

The invention discloses a method for rapidly evaluating the quality of an andrographis paniculata medicinal material, which adopts a near infrared spectrum technology, can be used for determination after a sample is crushed, does not need complex pretreatment operation on the sample, is simple, convenient and rapid, only needs several minutes for detection, does not need chemical reagents for dissolution, and can be used for rapidly evaluating the quality of the andrographis paniculata medicinal material. The contents of chlorogenic acid, andrographolide and dehydroandrographolide can be measured at the same time, and the content of limited components in the andrographis paniculata medicinal material is comprehensively evaluated.