Method for extracting andrographolide from andrographis paniculata

A technology of andrographolide and andrographolide, which is applied in the field of extracting andrographolide by combining plant tissue crushing and enzymatic hydrolysis, can solve the problems of expensive equipment and unsuitability for large-scale production, and achieve low cost, reduced resistance, and high extraction rate high effect

Inactive Publication Date: 2013-12-11
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ethanol reflux extraction is the most commonly used, but the disadvantage of this method is the purity of andrographolide in the extract; supercritical extraction equipment is expensive, and it is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Get the whole herb of Andrographis paniculata, add 70% ethanol solution of 1 times the weight ratio to it, pulverize with the plant tissue crushing method, the rotating speed of the plant tissue crushing extractor is 4000r / min, and the extraction time is 2min; add 8 times of 70% ethanol to the above extract % ethanol, adjust the pH value to 4.5, then add 0.04% cellulase as the raw material, control the temperature at 45°C, stir, and enzymolysis time is 2h; the material after enzyme conversion is placed in an ultrasonic-microwave extractor, Ultrasound 600W (normally on), microwave power 200W, extraction time 10min, centrifuge the extract, take out the supernatant, add petroleum ether for extraction, remove fat-soluble impurities such as chlorophyll, concentrate, add 3 times the amount of Dissolved in 80% ethanol, the pretreated AB-8 macroporous adsorption resin was eluted with 70% ethanol solution, concentrated under reduced pressure, and then crystallized twice through e...

Embodiment 2

[0019] Get the whole herb of Andrographis paniculata, add 2 times of 80% ethanol solution in weight ratio, pulverize with the plant tissue crushing method, the plant tissue crushing extractor speed is 3000r / min, and the extraction time is 1.5min; add 10 times of 80% ethanol, adjust the pH value to 5.0, then add 0.05% cellulase as the raw material, control the temperature at 50°C, stir, and enzymolysis time is 2.5h; put the material after enzyme conversion in an ultrasonic-microwave extractor Medium, ultrasonic wave 600W (normally on), microwave power 300W, extraction time 15min, centrifuge the extract, take out the supernatant, add petroleum ether for extraction, remove fat-soluble impurities such as chlorophyll, concentrate, add 3 times The amount of 80% ethanol was dissolved, the pretreated D-101 macroporous adsorption resin was eluted with 80% ethanol solution, concentrated under reduced pressure, and then crystallized twice through ethanol to obtain the andrographolide prod...

Embodiment 3

[0021] Get the whole herb of Andrographis paniculata, add 1.5 times of 75% ethanol solution by weight, pulverize with the plant tissue crushing method, the plant tissue crushing extractor rotating speed is 2000r / min, and the extraction time is 1min; add 9 times of 75% ethanol to the above extract % ethanol, adjust the pH value to 5.5, then add 0.06% cellulase as the raw material, control the temperature at 55°C, stir, and enzymolysis time is 3h; the material after enzyme conversion is placed in an ultrasonic-microwave extractor, Ultrasound 600W (normally on), microwave power 250W, extraction time 20min, centrifuge the extract, take out the supernatant, add petroleum ether for extraction, remove fat-soluble impurities such as chlorophyll, concentrate, add 3 times the amount of Dissolved in 80% ethanol, the pretreated DA201-A macroporous adsorption resin was eluted with 75% ethanol solution, concentrated under reduced pressure, and repeated crystallization by ethanol twice to obt...

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Abstract

The invention relates to a method for extracting andrographolide from andrographis paniculata and in particular relates to a method for extracting andrographolide by employing the combination of a plant tissue crushing method and enzymolysis. The processing steps comprises: crushing the whole andrographis paniculata by the plant tissue crushing extraction method, carrying out enzymolysis on the crushed andrographis paniculata, extracting by the assistance of ultrasonic wave and microwave, filtering, extracting by petroleum ether, purifying by macroporous resin, concentrating under reduced pressure and re-crystallizing, thus obtaining the andrographolide with high purity. The method for extracting andrographolide from andrographis paniculata carries out crushing at room temperature, and is free from damage to ingredient, saves time, has high efficiency and saves energy, is simple and convenient to operate, beneficial to environment protection, high in extraction efficiency and purity, and applicable to production in large scale.

Description

Technical field [0001] The present invention involves a method of extracting wearing lotus glycolin from the heart lotus, especially a method that uses the combination of plant tissue crushing and enzymatic disintegration to extract the wearing lotus glycolin. Background technique [0002] Wearing lotus dectin, alias, wearing heart lotus Byin, belongs to the two -ring dihe compound, the appearance is white or slightly yellow rectangular crystals, without odor and bitter taste.Difficult to dissolve in water, soluble in methanol, chloroform, acetone, ether, exist in wearing heart lotus (also known as the joy, chopped snake grass, bitter grass), has antibacterial and antiviral effects, which can be used to treat liver disease and intestinal diseases, Kidney stones, etc.It is found in the study that there is a significant difference in stability in different environments in different environments: the lower the temperature, the better the stability of the wearing lotus glow;Its insta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/60
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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