2306 results about "Succinates" patented technology

The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions encoding an enzyme associated with growth-coupled production of succinate when an activity of the enzyme is reduced, whereby the one or more gene disruptions confers stable growth-coupled production of succinate onto the non- naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling succinate production to growth of the microorganism, the set of metabolic modifications comprising disruption of one or more genes selected from the set of genes comprising: (a) adhE, ldhA; (b) adhE, ldhA, acka-pta; (c) pfl, ldhA; (d) pfl, ldhA, adhE; (e) acka-pta, pykF, atpF, sdhA; (f) acka-pta, pykF, ptsG, or (g) acka-pta, pykF, ptsG, adhE, ldhA, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of succinate. Additionally provided is a non-naturally occurring microorganism having the genes encoding the metabolic modification (e) acka-pta, pykF, atpF, sdhA that further includes disruption of at least one gene selected from pyka, atpH, sdhB or dhaKLM; a non-naturally occurring microorganism having the genes encoding the metabolic modification (f) ackA-pta, pykF, ptsG that further includes disruption of at least one gene selected from pykA or dhaKLM, or a non-naturally occurring microorganism having the genes encoding the metabolic modification (g) ackA-pta, pykF, ptsG, adhE, ldhA that further includes disruption of at least one gene selected from pykA or dhaKLM. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of succinate also is provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring succinate production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring succinate production.

The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.

The invention relates to a hybrid succinate production system that has a high capacity to produce succinate under aerobic and anaerobic conditions. The metabolic engineering of a hybrid bacterial succinate production system that can function under both aerobic and anaerobic conditions makes the production process more efficient, and the process control and optimization less difficult.

A powdered tocopheryl polyethylene glycol succinate (TPGS™) having an average particle size of less than about 1000 microns. In one embodiment, the powdered tocopheryl polyethylene glycol succinate is prepared by a process that includes atomizing a fluidic tocopheryl polyethylene glycol succinate into an environment suitable for solidifying the atomized tocopheryl polyethylene glycol succinate. In another embodiment, the powdered tocopheryl polyethylene glycol succinate is prepared by a process of applying a force to a solid tocopheryl polyethylene glycol succinate starting material that is sufficient to produce a powdered product.

The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.

In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

The present invention relates to beverage compositions that have the added benefit of providing a water-soluble source of Vitamin E. For example, d,l- or d-α-tocopheryl polyethylene glycol 1000 succinate (such as Vitamin E TPGS™) may be used to provide tocopheryl (i.e., vitamin E) content to beverages while concurrently maintaining beverage clarity when desired. Various additives may also be used in the beverage compositions of the present invention, such as flavoring agents, colorants, preservatives, sweeteners as well as other vitamins, minerals and/or electrolytes.

A heavy oil cleaning composition comprising: a) a blend of dibasic esters comprising dialkyl methylglutarate and at least one of a dialkyl adipate or dialkyl ethylsuccinate; b) at least one terpene; and c) at least one surfactant. Also described are methods for delivering a solvent at reduced concentration comprising the steps of: a) obtaining a terpene-based solvent; and b) mixing the terpene-based solvent with a carrier fluid (the carrier fluid comprising a microemulsion of i) a blend of dibasic esters selected from the group consisting of dialkyl methylglutarate, dialkyl adipate, dialkyl ethylsuccinate, dialkyl succinate, dialkyl glutarate and any combination thereof, ii) at least one surfactant selected from the group consisting of a terpene alkoxylate, an alcohol alkoxylate and any combination thereof; and iii) water) in order to obtain a mixture to clean heavy oils.

The present invention relates to a method for preparing lactone polymers, carbonate polymers, lactone-carbonate block copolymers and lactone-arbonate random copolymers via a ring-opening addition reaction of a lactone monomer, a cyclic carbonate monomer or a mixture thereof using an initiator and in the presence of a specific titanium-type Lewis acid catalyst. The resulting polymers have a molecular weight distribution (Mw/Mn) approximately equal to 1 or a extremely high purity of single-structure components. The polymer molecules range in an oligomer region to a molecular weight of approximately 200,000. The present invention also relates to a method for preparing lactone polymers, carbonate polymers and lactone-carbonate copolymers having a narrow molecular weight distribution and which can be obtained via a continuous polymerisation of a lactone monomer and/or a cyclic carbonate monomer using an initiator in an extruder in the presence of a specific titanium-type Lewis acid catalyst or an aluminium-type Lewis acid catalyst. The present invention also relates to these resulting polymers.

Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.

The invention relates to a series of polyglycoside derivatives that contain water-soluble sulfosuccinate groups introduced into the molecule by reaction with the hydroxyl groups present in the starting polyglycoside molecule, with the chloro material. The preferred products have more than one water-soluble group per molecule and are made with mild reagents to avoid discoloration and mal odor. The most preferred products have between 2 and 3 functional groups per molecule.

The present invention is linseed oil microcapsule powder and its preparation, and features that modified polysaccharide, sodium octyl alkenyl succinate starch, is used as capsule wall material for forming microcapsule in preparing linseed oil microcapsule powder. The preparation process includes adding linseed oil into the solution of sodium octyl alkenyl succinate starch, stirring to form homogeneous emulsion, high pressure homogenizing to obtain linseed oil emulsion of granularity smaller than 1000 nm, and final spray drying or freeze drying to obtain white linseed oil microcapsule powder with high stability and high flowability. The linseed oil microcapsule powder has high linseed oil activity, high stability and high bioavailability, and may be applied in food and health product.

The present invention relates to one kind of high molecular weight polybutanediol succinate and its preparation process, and features that bihydroxy terminated butanediol succinate, especially low molecular weight bihydroxy terminated butanediol succinate, as the pre-polymer and diisocyanate as the chain extender in 0.1-13 % are molten and reacted to prepare polybutanediol succinate with low cost, great molecular weight, narrow molecular weight distribution and excellent mechanical performance. The polybutanediol succinate has number average molecular weight greater than 5.5x10<4>, molecular weight distribution of 1.4-1.8, tensile strength of 39-58 MPa, and elongation at break of 300-700 %.

A coating dispersion soluble in the lower digestive tract which is prepared by blending a hydroxypropyl methylcellulose acetate succinate (HPMCAS) soluble at around pH 7 with a conventional plasticizer and an anion surfactant and further adding an acid, wherein the HPMCAS has an average particle size of 10 μm or less and is dispersed at a concentration of 2 to 20% by weight, and the acid is used in an amount of 1 to 10 parts by weight per 100 parts by weight of HPMCAS, and a sustained release coated preparation capable of releasing a medicament in the large intestine at the lower digestive tract, which is prepared by coating a medicament-containing solid preparation such as a granular core with the coating dispersion.

A method for administering an antioxidant composition to humans according to their age and sex is disclosed wherein the method comprises administering to said humans a daily dose of a multiple antioxidant micronutrient composition comprising vitamin A (palmitate), beta carotene (from natural d. salina), vitamin C (calcium ascorbate), vitamin D-3 (cholecalciferol), natural source vitamin E including both d-alpha tocopheryl and d-alpha tocopheryl acid succinate, thiamine mononitrate, riboflavin, niacinamide ascorbate, d-calcium pantothenate, pyridoxine hydrochloride, cyanocobalamin, folic acid (folacin), d-biotin, selenium (1-seleno methionine), chromium picolinate, zinc glycinate, calcium citrate, and magnesium citrate. For persons over the age of about 51, the composition preferably further comprises one or more of co-enzyme Q₁₀, N-acetyl cysteine, and alpha lipoic acid. Preferably, also, vitamin D is added for women over the age of about 36.

The invention discloses a continuously-produced full-degradable starch-based plastic alloy and a preparation method thereof. The starch-based plastic alloy contains the following components in parts by weight: 40-90 parts of starch, 5-60 parts of poly(butylene succinate), 5-60 parts of polylactic acid, 10-40 parts of plasticizer, 0.1-10 parts of compatibilizer and 1-10 parts of processing acid. The preparation method comprises the steps of mixing starch, plasticizer and part of processing aid, and extruding to obtain thermoplastic starch particles; then, mixing the thermoplastic starch particles with poly(butylene succinate), polylactic acid, compatibilizer and the residual processing aid, and extruding to obtain the starch-based plastic alloy. The starch-based plastic alloy disclosed by the invention is favorable in hydrophobicity and strength, good in component compatibility, favorable in thermoplastic processability, capable of realizing continuous production and processing as well as simple and feasible in technical process.

The invention relates to a polylactic acid toughening modifier in the technical field of high polymer material and a preparation method thereof. Lactide ring-opening polymerization is initiated on the terminal hydroxyl of poly (butylene succinate), and purification is carried out, thus obtaining the polylactic acid toughening modifier, the chemical structural formula is as follows. The modifier of the invention prepared by adopting degradable material can solve transport phenomenon and diosmose in the prior art.

The present invention provides vitamin E succinate (VES)-stabilized compositions, methods for the preparation thereof and methods useful for the in vivo delivery of substantially water insoluble and optionally chemically unstable pharmacologically active agents (such as docetaxel).

A multiparticulate O-desmethylvenlafaxine (ODV) succinate or formate is described. Methods of treating depression and reducing the gastrointestinal side-effects of ODV are also described.