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36 results about "Lactate Dehydrogenase A Gene" patented technology

Lactate dehydrogenase A (LDHA) is an enzyme which in humans is encoded by the LDHA gene. It is a monomer of lactate dehydrogenase, which exists as a tetramer. The other main subunit is lactate dehydrogenase B (LDHB).

Methods and organisms for the growth-coupled production of succinate

InactiveUS20070111294A1Stable growth-coupled productionFungiBacteriaMicroorganismSuccinic acid
The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions encoding an enzyme associated with growth-coupled production of succinate when an activity of the enzyme is reduced, whereby the one or more gene disruptions confers stable growth-coupled production of succinate onto the non- naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling succinate production to growth of the microorganism, the set of metabolic modifications comprising disruption of one or more genes selected from the set of genes comprising: (a) adhE, ldhA; (b) adhE, ldhA, acka-pta; (c) pfl, ldhA; (d) pfl, ldhA, adhE; (e) acka-pta, pykF, atpF, sdhA; (f) acka-pta, pykF, ptsG, or (g) acka-pta, pykF, ptsG, adhE, ldhA, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of succinate. Additionally provided is a non-naturally occurring microorganism having the genes encoding the metabolic modification (e) acka-pta, pykF, atpF, sdhA that further includes disruption of at least one gene selected from pyka, atpH, sdhB or dhaKLM; a non-naturally occurring microorganism having the genes encoding the metabolic modification (f) ackA-pta, pykF, ptsG that further includes disruption of at least one gene selected from pykA or dhaKLM, or a non-naturally occurring microorganism having the genes encoding the metabolic modification (g) ackA-pta, pykF, ptsG, adhE, ldhA that further includes disruption of at least one gene selected from pykA or dhaKLM. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of succinate also is provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring succinate production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring succinate production.
Owner:GENOMATICA INC

Methods and Organisms for Growth-Coupled Production of 3-Hydroxypropionic Acid

The invention provides a non-naturally occurring microorganism having one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory coupling 3-hydroxypropionic acid production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 3-hydroxypropionic acid onto the non-naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling 3-hydroxypropionic acid production to growth of the microorganism, the set of metabolic modifications having disruption of one or more genes including: (a) the set of genes selected from: (1) adhE, ldhA, pta-ackA; (2) adhE, ldhA, frdABCD; (3) adhE, ldhA, frdABCD, ptsG; (4) adhE, ldhA, frdABCD, pntAB; (5) adhE, ldhA, fumA, fumB, fumC; (6) adhE, ldhA, fumA, fumB, fumC, pntAB; (7) pflAB, ldhA, or (8) adhE, ldhA, pgi in a microorganism utilizing an anaerobic β-alanine 3-HP precursor pathway; (b) the set of genes selected from: (1) tpi, zwf; (2) tpi, ybhE; (3) tpi, gnd; (4) fpb, gapA; (5) pgi, edd, or (6) pgi, eda in a microorganism utilizing an aerobic glycerol 3-HP precursor pathway; (c) the set of genes selected from: (1) eno; (2) yibO; (3) eno, atpH, or other atp subunit, or (4) yibO, atpH, or other atp subunit, in a microorganism utilizing a glycerate 3-HP precursor pathway, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of 3-hydroxypropionic acid. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of 3-hydroxypropionic acid is further provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring 3-hydroxypropionic acid production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring 3-hydroxypropionic acid production.
Owner:GENOMATICA INC

High-yield succinic acid strain and its application

The invention relates to a bacterial strain for the high yield of succinic acid and application thereof. Escherichia coli is modified in order to remove or decrease the activities of adhE, ackA, pta, focA, pflB, iclR, ldhA, poxB and pepc genes; and the gene knockout bacterial strain then receives ultraviolet mutagenesis, and is screened by culture medium with glucose as the only carbon source, so that an engineering bacterial strain which can produce the succinic acid on basal medium and with the glucose as the only carbon source by anaerobic fermentation can be obtained. The invention adopts the succinic acid-producing escherichia coli as the starting strain, and by means of a rational design, the inveniton obtains the engineering bacterial strain with the glucose as the only carbon source theoretically, which carries out anaerobic fermentation in the basal medium and only produces the succinic acid and no other byproducts. Then by means of irrational metabolic evolution integrating ultraviolet mutagenesis with growth screening, a mutant strain for producing the succinic acid is obtained. The bacterial strain disclosed by the invention nearly produces no other heteroacids in the fermentation of the succinic acid, so the production cost of the succinic acid can be remarkably reduced.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Recombinant Escherichia coli for synthesizing S-1,2-propanediol from L-lactic acid and construction method thereof

The invention discloses a recombinant Escherichia coli for synthesizing S-1,2-propanediol from L-lactic acid and a construction method thereof. The method comprises the following steps: 1) respectively substituting lldD gene, adheE gene and ackA-pta gene in Escherichia coli BW25113-DELTApoxB with coding gene of 3-hydroxypropionate dehydrogenase, coding gene of coenzyme A-dependent succinic semialdehyde dehydrogenase and coding gene of propionyl coenzyme A transferase to obtain recombinant bacterium BWPDO1; 2) knocking out ldhA gene and dld gene of the BWPDO1 to obtain recombinant bacterium BWPDO2; and 3) transferring coding gene of pyruvate decarboxylase and coding gene of NAD-dependent acetaldehyde coenzyme A dehydrogenase into the BWPDO2 to obtain recombinant bacterium BWPDO3 which is the recombinant Escherichia coli. The experiment proves that the recombinant Escherichia coli can generate S-1,2-propanediol by converting L-lactic acid; and by using 200 mM of sodium lactate as the substrate, the culture is performed at 37 DEG C for 24 hours to generate 3.4 mM of S-1,2-propanediol. The recombinant Escherichia coli lays foundation for enhancing the yield and conversion rate of the biosynthetic S-1,2-propanediol.
Owner:普立思生物科技有限公司
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