Biocatalyst for producing d-lactic acid

A lactic acid and microorganism technology, applied in the field of microorganisms producing D-lactic acid, can solve problems such as inactivation of aspA, difficulty in decomposing aspartic acid or glutamine, research on the amount of fumaric acid production, and the like. The effect of suppressing by-products and reducing purification costs
CN1856577AActive Publication Date: 2006-11-01MITSUI CHEM INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MITSUI CHEM INC
Publication Date
2006-11-01

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Abstract

It is intended to provide a process for highly producing D-lactic acid. It is also intended to provide a process for highly selectively producing D-lactic acid having a high optical purity with the formation of little organic acids as by-products. D-Lactic acid is produced by culturing a microorganism having inactivated or lowered pyruvate formate-lyase activity and elevated Escherichia coli-origin NADH-dependent D-lactic acid dehydrogenase (ldhA) activity, a microorganism having inactivated or lowered FAD-dependent D-lactic acid dehydrogenase activity, or a microorganism as described above having a TCA cycle, inactivated or lowered malic acid dehydrogenase activity and inactivated or lowered aspartic acid ammonia-lyase activity. The ldhA activity is elevated by ligating a gene encoding ldhA to a promoter of a gene controlling the expression of a protein, which participates in a glycolysis system, a nucleic acid biosynthesis system or an amino acid biosynthesis system, on genome.
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Description

technical field

[0001] The present invention relates to a microorganism capable of producing D-lactic acid with high selectivity and high efficiency, and a method for producing D-lactic acid using the microorganism. Specifically, it relates to a method for efficiently producing high-purity lactic acid, and particularly relates to an efficient method for producing D-lactic acid with a small accumulation of pyruvic acid.

[0002] The present invention also relates to a method for producing D-lactic acid, characterized in that the method uses a microorganism with inactivated or reduced activity of FAD-dependent D-lactate dehydrogenase.

[0003] In addition, the present invention also relates to a D-lactic acid-producing microorganism that does not produce succinic acid or fumaric acid as an impurity when producing D-lactic acid, and a method for producing D-lactic acid using the same. Background technique

[0004] The biodegradable polymer polylactic acid triggers CO 2 While ...

Claims

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