31 results about "Structural gene" patented technology

The invention provides a mutant of enterovirus 71 and virus-like particles thereof, and relates to the field of biological medicines. The enterovirus 71 type virus-like particle is obtained by modifying structural genes VP0, VP1 and VP3 of virus capsid protein by utilizing computational structure biology and completing self-assembly in vivo through a hansenula polymorpha expression system. Compared with the unmodified enterovirus 71 type mutant and the virus-like particles thereof, the enterovirus 71 type mutant and the virus-like particles thereof have the advantages that the immunogenicity can be obviously improved, and the clinical application prospect is relatively high.

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

The invention discloses a key gene AaMYB114 closely linked with the color character of an actinidia arguta fruit and application of the key gene AaMYB114. The AaMYB114 gene disclosed by the invention is positioned at 5237694-5239966bp of a chromosome 9 of an actinidia arguta genome, and the full length of the AaMYB114 gene is 2273bp. According to the application, all-red type and all-green type actinidia arguta are used as materials for transcriptome sequencing, and the gene AaMYB114 related to the formation of the peel color of the actinidia arguta is explored. Instantaneous conversion of the actinidia arguta fruit proves that the overexpression of the AaMYB114 increases the anthocyanin content in the actinidia arguta peel. Yeast one-hybridization and dual-luciferase report tests prove that the AaMYB114 not only interacts with a promoter of AaGST, activates the expression of the promoter and regulates anthocyanin transport of peel tissues of actinidia arguta, but also is combined with promoters of anthocyanin synthesis structural genes AaPAL, AaCHI and AaF3H and activates the expression of the promoters to promote anthocyanin synthesis. Therefore, the AaMYB114 gene plays a dual regulation and control role in the formation of peel color and luster, and can be used for cultivating red-peel actinidia arguta.

The application discloses a kind of compositions, reagents and its detection method for high-throughput gene sequencing, belong to gene sequencing technical field, the composition includes Tris-HCl buffer 80-120 parts, potassium chloride 40-60 parts, ammonium sulfate 10-20 parts, magnesium chloride 15-25 parts, dNTPs mixed solution 30-50 parts, heat-stable DNA polymerase mutant 8-15 parts, PCR promoter complex 5-12 parts, modified iridium quinoline ketone complex 1-5 parts, glycerol 150-250 parts, Tween-20 1-3 parts, EDTA disodium salt 0.5-2 parts and nuclease-free ultrapure water, by introducing the modified iridium quinoline ketone complex of innovation, effectively solve the technical problems of low amplification efficiency, poor multiplex PCR reaction uniformity and high non-specific amplification background for high GC content, complex secondary structure genomic template, significantly improve the specificity of amplification, uniformity and the quality and reliability of final sequencing data.

The present invention relates to a method for searching for a structural gene of glass, including the following steps: determining atomic species for structure search according to the glass system; screening structure on the basis of the first principle to screen out compounds that can be formed by the interaction among each of the atoms; comparing the formation energy and the phonon spectrum of each compound to obtain stable compounds; and constructing a metastable composition diagram of a glass system according to the stable compounds, in metastable composition diagram, a micro-structural unit of a glassy compound near a target glass composition point is the structural gene of glass in the metastable glass composition diagram; designing glass properties based on the characteristics of the structural genes; and realizing the design of high-performance glasses through a hot-melt method.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The invention discloses a gingko GbC4H1 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the gingko GbC4H1 gene disclosed by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the gingko GbC4H1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: constructing an expression vector of the gingko GbC4H1 gene, and transforming the expression vector into the nicotiana benthamiana; and cultivating and screening to obtain a transgenic plant with increased flavonoid metabolic substance content. Compared with a control group, the expression level of regulation and control transcription factors of flavone synthesis pathway related genes and flavone synthesis pathway structural genes in a transgenic line is remarkably improved. The content of flavonoid metabolites in a transgenic strain is increased, and compared with a control group, 14 kinds of differential metabolites are up-regulated and 20 kinds of differential metabolites are down-regulated.

The present invention relates to recombinant batroxobin and a method for its preparation on an industrial scale with high expression level. Specifically, the method for preparing recombinant batroxobin according to the present invention is to artificially synthesize the structural gene sequence of batroxobin, then use Pichia pastoris to clone and express to screen and obtain the seed liquid of a high-expression engineering strain, and then ferment and culture to prepare recombinant batroxobin according to the following operations: preparation of primary seed liquid, preparation of secondary seed liquid, inoculation and pre-culture in a fermenter, glycerol fed-batch culture, methanol fed-batch induction culture, concentration to obtain a fermentation concentrate containing recombinant batroxobin and optionally purifying it. This recombinant batroxobin can be clinically used for acute cerebral infarction, for improving ischemic symptoms caused by various occlusive vascular diseases (such as thromboangiitis obliterans, deep phlebitis, pulmonary embolism, etc.), and for improving peripheral and microcirculation disorders (such as sudden deafness, motion sickness). The expression level of batroxobin by the method of the present invention is high, and industrial-scale production can be easily achieved.

The application discloses a kind of peptidoglycan recognition protein-D, preparation method and application, belong to biological medicine technical field.The application obtains natural peptidoglycan recognition protein-D from tussah by protein separation and purification technology, realizes the expression of peptidoglycan recognition protein-D and its derivative or analogue or partial fragment gene in host cell after resolving its primary structure (gene and protein) using genetic engineering technology, and the antibody of purified recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment is obtained by immunizing animal.The natural, recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment and its antibody of the application can be widely used for prevention, detection diagnosis, treatment and other biological medicine fields of microorganism, microorganism related molecular pattern.

The application belongs to the technical field of genetic engineering, and provides a gene OsMYB1 and application of the gene in negative regulation of anthocyanin synthesis; the cDNA sequence of the gene OsMYB1 is shown as SEQ ID NO. 1. The application proves by systematic molecular biology experiments that OsMYB1 can inhibit the expression of OsDFR by combining with the promoter region of the key structural gene OsDFR of anthocyanin synthesis, thereby inhibiting the synthesis of anthocyanin. Meanwhile, OsMYB1 can also form an MBW ternary complex with OsB2 and OsPAC1, competitively inhibits the synthesis of anthocyanin, and indicates that the transcription factor OsMYB1 is a negative regulation factor of anthocyanin synthesis in rice, and knocking out the same can increase the content of anthocyanin in rice, thereby providing certain theoretical basis for cultivating high-quality purple rice with high anthocyanin content.

The application belongs to the field of agricultural microorganism application, and particularly relates to a primer group, a reagent or a kit, and a strain screening method and application adopting the same. The primer group is designed according to a conserved sequence of a structural gene fragment in an iturin A synthesis gene cluster. The structural gene in the iturin A synthesis gene cluster includes: ituA gene, ituB gene, and ituC gene. The primer group includes: primer pair I, primer pair II, and primer pair III. The primer group of the application is designed according to a conserved sequence of a structural gene fragment in an iturin A synthesis gene cluster, so as to specifically amplify a key structural gene ( ituA gene, ituB gene, and ituC gene) in the iturin A synthesis gene cluster. By using the primer group, combining a PCR amplification technology and an electrophoresis detection technology, and then rapidly, accurately and low-costly screening a bacillus positive strain capable of producing iturin A, the application has a wide application prospect.​​​​​​​​​​​​

The application discloses a ginkgo biloba L. GbC4H1 gene, a coding protein and application thereof, and relates to the technical field of plant genetic engineering.The ginkgo biloba L. GbC4H1 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the coding protein is as shown in SEQ ID NO.2.The expression vector of the ginkgo biloba L. GbC4H1 gene is constructed and transformed into Nicotiana benthamiana; and a transgenic plant with increased flavonoid metabolite content is obtained through cultivation and screening.Compared with a control group, the expression level of a transcription factor of a flavone synthesis pathway related gene and a flavone synthesis pathway structural gene in the transgenic strain is significantly increased.The flavonoid metabolite content in the transgenic strain is increased, and compared with the control group, 14 kinds of differential metabolites are up-regulated and 20 kinds of differential metabolites are down-regulated.

This invention discloses a multi-objective evolutionary modeling method for fake data attacks in power system CPS (Computer-Powered Systems), belonging to the field of data attack technology. It solves the problem of insufficient attack flexibility in existing technologies, which fails to provide a foundation for power system defense. This invention models attacks through a three-step attack-defense game: attack gene generation, optimization, and evolution. In the gene generation stage, gene encoding technology is used to construct attack gene units from the decoupled attack offset and tag policy matrix, and these units are then assembled into base pair structures by aligning and splicing them. In the gene optimization stage, a multi-objective fitness function is constructed based on concealment and attack effectiveness objectives. Using residuals and attack effectiveness as objectives, a multi-objective collaborative screening between destructiveness and concealment is achieved to obtain optimized genes. In the gene evolution stage, multi-point crossover and single-point crossover operations are used for the attack chain and concealment chain of the optimized genes, respectively, to enhance the attack effectiveness.

The invention discloses a method for identifying three gene loci related to nicotine accumulation. The method comprises the following steps: step 1, genotype detection and NILs construction: adopting a CAPS marker, designing a specific primer to amplify Myc2a, and performing Hpy188I enzyme digestion and electrophoresis identification on a PCR product to identify 5bp deletion mutation; carrying out allele typing by adopting a KASP marker, and additionally detecting ERF115 and ERF189 deletion of a BC6F2 generation by using two pairs of primers; the method comprises the following steps: obtaining three homozygous near-isogenic lines (NILs): K326 nic1 / nic2, K326 myc2a and K326 nic1 / nic2 / myc2a; the three-gene-locus combination effect is systematically analyzed for the first time, and it is proved that nicotine can be reduced to 0.95 mg.g <-1 > through the combined genotype, and the nicotine is close to the supervision target; according to the invention, it is found for the first time that Myc2a partially regulates and controls alkaloid synthesis through a JA signal pathway (does not interact with * Nic1 / Nic2 *); the invention provides a practical breeding tool for breeding a low-risk tobacco variety (nicotine lt; 1.0 mgg <-1 >), and development defects caused by direct editing of structural genes are avoided.

This invention discloses the application of the NbMYB159 gene in the positive regulation of the MVA metabolic pathway in Nicotiana benthamiana, belonging to the field of genetic engineering technology. The technical solution includes the application of the NbMYB159 gene in the positive regulation of the MVA metabolic pathway in Nicotiana benthamiana. The nucleotide sequence of the gene is shown in SEQ ID NO: 1. The NbMYB159 gene can enhance the metabolic flux of the MVA pathway by activating the expression of key structural genes in the Nicotiana benthamiana MVA pathway. This invention discovers that NbMYB159 can specifically bind to the promoter regions of NbHMGR and NbHMGS, activating the expression of all genes in the MVA pathway. Transient overexpression of NbMYB159 in Nicotiana benthamiana significantly increases the content of endogenous FPP in Nicotiana benthamiana. Stable overexpression lines significantly increase the content of endogenous FPP in Nicotiana benthamiana without affecting the biomass of Nicotiana benthamiana, while transcriptional repression overexpression lines significantly reduce the content of FPP. This discovery is of great significance for the engineering modification of the Nicotiana benthamiana chassis and can effectively solve the problem of low production efficiency of sesquiterpenes and triterpenes in Nicotiana benthamiana.

Provided is a protein co-expression vector system. The protein co-expression vector system comprises nucleic acids of two target genes and a bidirectional promoter, and further comprises the following structure: gene B-promoter B-enhancer-promoter A-gene A. The protein co-expression vector enables expression levels of different target genes to be relatively balanced, and provides a new strategy for multiple gene co-expression.

The application discloses serine protease homolog SLP-1 and a preparation method and application thereof, and belongs to the technical field of biological medicine. The natural serine protease homolog SLP-1 is obtained by using protein separation and purification technology to separate and purify the natural serine protease homolog SLP-1 from a tussock moth. After the primary structure (gene and protein) of the serine protease homolog SLP-1 is analyzed, the expression of the serine protease homolog SLP-1 and derivatives or analogs or partial fragments thereof in a host cell is realized by using genetic engineering technology. The recombinant serine protease homolog SLP-1 and derivatives or analogs or partial fragments thereof are obtained by purification. Animals are immunized to obtain corresponding antibodies. The natural, recombinant serine protease homolog SLP-1 and derivatives or analogs or partial fragments thereof and the antibodies thereof can be widely applied to the fields of prevention, detection, treatment drugs and the like for microorganisms.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant foal foal xylose as well as a construction method and application thereof. The recombinant foal foal capable of improving the bacterial cellulose yield and the xylose utilization amount is constructed by taking the foal foal foal as an original strain and overexpressing a xylose isomerase gene xylA, a xylulokinase gene xylB, a phosphopentose pathway key gene TKL and a structural gene BcsD. Under the fermentation conditions of pure xylose and xylose and glucose, the bacterial cellulose yields of the bacterial strain can reach 9.51 g / L and 8.1 g / L respectively and are increased by 553.23% and 44.75% respectively compared with original bacteria, and the highest xylose consumption of the bacterial strain under the fermentation conditions of pure xylose can reach 11.18 g / L. According to the method, the utilization rate of xylose is remarkably increased, the production efficiency of bacterial cellulose is improved, and a feasible solution is provided for replacing efficient conversion of traditional biomass resources with glucose as a carbon source.

This invention belongs to the field of genetic engineering technology and provides the rice transcription factor OsMYB5 and its application in regulating anthocyanin synthesis; the cDNA sequence of the rice transcription factor OsMYB5 is shown in SEQ ID NO.1. This invention, through systematic molecular biology experiments, confirms that OsMYB5 promotes the expression of OsDFR and OsF3'H, key structural genes for anthocyanin synthesis, by binding to their promoter regions, thereby promoting anthocyanin synthesis. Simultaneously, OsMYB5 can also form an MBW ternary complex with OsB2 and OsPAC1, regulating anthocyanin synthesis. Furthermore, experiments have confirmed that the transcription factor OsMYB5 is a positive regulator of rice anthocyanin synthesis, and its overexpression can increase the anthocyanin content of rice, providing a theoretical basis for cultivating high-quality purple rice with high anthocyanin content.

The disclosure provides a mutant of enterovirus 71 and a virus-like particle thereof, and relates to the field of biological medicine.The virus-like particle of enterovirus 71 in the disclosure is obtained by modifying the structural genes VP0, VP1 and VP3 of the virus capsid protein by using computational structural biology, and completing self-assembly in vivo by a Hansenula polymorpha expression system.The mutant of enterovirus 71 and the virus-like particle thereof in the disclosure can significantly improve immunogenicity compared with the unmodified one, and have a high clinical application prospect.

The invention discloses a transcription factor BnaMYB30 and application thereof, and belongs to the technical field of gene engineering. The invention provides application of a BnaMYB30 gene or protein to regulation and control of drought resistance of rape and / or regulation and control of anthocyanin content of rape under drought stress. The BnaMYB30 is combined with a BnaUGT75C1 promoter and a BnaFLS1 promoter to participate in regulation and control of glycosylation of anthocyanin and flavonol synthesis, and accumulation of the anthocyanin is remarkably promoted by activating key structural genes CHI, F3H and DFR in a flavonoid synthesis pathway. The BnaMYB30 overexpression strain lacks effective ABA and JA hormone signal regulation and control, and the stress response mode is single, so that the antioxidant enzyme activity is reduced, the active oxygen scavenging capacity is reduced, and finally the drought sensitive phenotype is shown.

The present invention discloses a method for mining key genes for the synthesis of terpenoid compounds in Zanthoxylum armatum leaves based on third-generation full-length and second-generation transcriptome sequencing, including: determining the components and contents of terpenoid compounds in the leaves of Zanthoxylum armatum DC. var. ferrugineum Rehd. and its bud mutant variety Rongchang thornless Zanthoxylum armatum at three different developmental stages; extracting total RNA from the leaves to construct a sequencing library, performing data analysis and functional annotation. CDS, SSR, lncRNAs and As events were predicted, differentially expressed genes were analyzed, and differentially expressed genes of structural genes and transcription factors related to terpenoid biosynthesis were screened; a co-expression gene network was constructed, and the FPKM values of hub genes were analyzed for correlation with the contents of terpenoid compounds, and genes positively correlated with the contents of terpenoid compounds were selected as genes related to their synthesis. The present invention successfully constructed a high-quality transcriptome of Zanthoxylum armatum, revealing 12 key genes that may be involved in regulating the synthesis of terpenoid compounds, laying a foundation for analyzing the molecular mechanism of terpenoid biosynthesis in Zanthoxylum armatum leaves.

The invention provides a protein co-expression vector system, which comprises two target genes and nucleic acid of a bidirectional promoter, and comprises the following structure: gene B-promoter B-enhancer-promoter A-gene A. According to the protein co-expression vector, the expression levels of different target genes are relatively balanced, and a new strategy is provided for co-expression of multiple genes.

The invention belongs to the field of agricultural microorganism application, and particularly relates to a primer group, a reagent or a kit, a strain screening method adopting the primer group, the reagent or the kit and application, and the primer group is designed according to a conserved sequence of a structural gene segment in a gene cluster synthesized by iturin A; wherein the structural genes in the iturin A synthetic gene cluster comprise an ituA gene, an ituB gene and an ituC gene; the primer group comprises a primer pair I, a primer pair II and a primer pair III. The primer group disclosed by the invention is designed according to a conserved sequence of a structural gene segment in an iturin A synthetic gene cluster, so that key structural gene segments (ituA gene, ituB gene and ituC gene) in the iturin A synthetic gene cluster can be specifically amplified, and by using the primer group and combining a PCR (Polymerase Chain Reaction) amplification technology and an electrophoresis detection technology, the detection sensitivity of the iturin A synthetic gene cluster is improved, and the detection sensitivity of the iturin A synthetic gene cluster is improved. The bacillus positive strain capable of producing the iturin A can be rapidly and accurately screened at low cost, and the application prospect is wide.

The present invention relates to a self-amplifying mRNA vaccine, including: a self-amplifying backbone comprising a live attenuated virus having a hairpin loop insert, and wherein at least one or more structural genes of the live attenuated virus have been replaced with at least one target antigen-encoding nucleic acid sequence.

The invention belongs to the technical field of tumor biology and organoids, and provides application of PARVA in promoting formation of tumor organoids. The PARVA comprises a PARVA protein or a PARVA coding gene; the application comprises application in preparation of a tumor organoid inducer, a tumor organoid culture kit and construction of a tumor organoid model. According to the invention, the formation efficiency and stability of the tumor organoid can be obviously improved, the difficulty in constructing the organoid with difficult-to-cultivate tumors such as lung cancer and bile duct cancer is solved, and a reliable model is provided for the fundamental research of the tumors; the constructed tumor organ model has a tissue structure, gene characteristics and drug responsiveness which are highly similar to in-vivo tumors, can be used for tumor generation mechanism research, antitumor drug high-throughput screening and clinical personalized treatment scheme evaluation, and has important scientific research value and clinical transformation significance.

The invention discloses application of a potato StHSF1 gene in alkaloid biosynthesis regulation and control, and belongs to the technical field of biology. According to the invention, potatoes with different pulp colors are studied, 48 alkaloids are identified and the accumulation dynamics of the alkaloids are determined, and the colored pulp potato clone is found to have richer alkaloid types and higher alkaloid content than a yellow pulp clone. A key structural gene is screened by systematically analyzing synthesis pathways of various alkaloids, transcription factors possibly participating in regulation are mined based on a co-expression network, and the StHSF1 gene is identified as a potential core hub regulation factor. Functional verification experiments show that transient overexpression of StHSF1 can significantly increase the total alkaloid content and specifically up-regulate the expression of 15 alkaloids. The invention provides a key genetic resource and a theoretical basis for cultivating a new potato variety with optimized alkaloid components, and is beneficial for increasing the nutritional value or reducing toxic alkaloid to guarantee the edible safety and the like by enriching functional alkaloid.

The invention discloses an application of an overexpression agent of a TRIM7 gene and / or a TRIM protein in preparation of an anti-rabies drug. According to the invention, by measuring the expression level of rabies virus structural genes / proteins in cells infected with rabies virus after overexpression of TRIM7 and healthy cells, it is determined that the overexpression agent of TRIM7 gene and / or TRIM protein can be used for resisting rabies, inhibiting the growth of rabies and / or reducing the titer of rabies virus, thereby reducing the proliferation of rabies virus. Therefore, the composition using the product containing the over-expressed TRIM7 gene and / or TRIM7 protein can effectively prevent and / or treat rabies.

The invention discloses a protein delivery system and preparation and application thereof, and belongs to the technical field of biology. The protein delivery system comprises a protein complex and a signal peptide, the protein complex comprises a structural protein and a regulatory factor, and the structural protein and the regulatory factor are from Xenorhabdus khoisanae. An XkCIS protein complex structure gene and a lysR gene from a Xenorhabdius khosanae MCB strain are expressed in escherichia coli, expression and assembly of the XkCIS protein complex in bacteria are achieved, further, XkCIS is modified, effect protein is selectively fused with signal peptide and loaded to the XkCIS protein complex under the guidance of the signal peptide, and the XkCIS protein complex is obtained. The targeted delivery of the effect protein can be realized.