4results about How to "Increase gene expression" patented technology

The present invention provides a hypoxia-triggered artificial transcription factor (HATF), and further provides a hypoxia-triggered transcription control system. The transcription control system comprises a nucleic acid sequence encoding the HATF, and a recognition element (RE). The hypoxia-triggered transcription control system comprises two sets of transcription control units linked upstream and downstream, wherein the upstream transcription control unit comprises a hypoxia-triggered transcription reaction element for controlling the HATF and a nucleic acid sequence encoding the HATF, and the downstream transcription control unit comprises an RE and a gene of interest. Co-regulation by the artificial transcription factor HATF and the recognition element RE can increase the expression of the gene of interest by a factor of one hundred.

The application provides a molecular marker related to porcine reproductive and respiratory syndrome resistance in a BCL2L15 gene and application thereof, the molecular marker rs331890123 is located at a 3'UTR region of a porcine BCL2L15 gene on a 106681000th base of a chromosome 4 of a porcine reference genome. The application takes the rs331890123 as a detection site, finds that the site polymorphism significantly influences the expression of the BCL2L15 gene, and further inhibits the proliferation of a porcine reproductive and respiratory syndrome virus. The application also establishes a kind of molecular marker polymorphism Tetra-primer ARMS-PCR rapid detection method related to porcine reproductive and respiratory syndrome resistance. The application provides a new SNP molecular marker for marker-assisted selection of porcine reproductive and respiratory syndrome disease resistance breeding.

Plant stress resistance genes GsZIP7 In the field of plant genetic engineering technology, this invention relates to the application of salt stress. To identify genes that regulate salt stress, this invention provides a plant stress-resistance gene for regulating plant salt stress tolerance. GsZIP7 The plant stress resistance gene GsZIP7 The nucleotide sequence is shown in SEQ ID NO.1, and a recombinant vector and recombinant bacteria containing the above gene were constructed to obtain transgenic plants; the plant stress resistance gene GsZIP7 Positive regulation of a plant's ability to tolerate salt stress can lay the foundation for research into breeding salt-tolerant transgenic plants.

The application discloses a broad-spectrum disease-resistant gene Rpl-avh23 of Nicotiana plumbaginifolia, and an encoding protein and application thereof. The application separates a disease-resistant gene Rpl-avh23 from the Nicotiana plumbaginifolia, the full-length cDNA of the gene is 2154 bp, the nucleotide sequence is shown as SEQ ID NO:1; the protein encoded by the gene is an intracellular immune receptor protein with a full length of 717 amino acids, and the amino acid sequence is shown as SEQ ID NO:2. The application finds that the Rpl-avh23 gene can improve the resistance of plants to oomycetes, fungi and / or viruses through transcription mode analysis and plant expression analysis, and therefore, the disease-resistant gene Rpl-avh23 can be applied to improving the disease resistance of plants or plant breeding.