10results about How to "Increase gene expression" patented technology

The invention discloses a gene PbrbHLH195 for regulating and controlling synthesis of pear fruit lignin. The CDS (Coding Sequence) of the gene is shown as SEQ ID NO. 1. An overexpression vector constructed by the gene is injected into a Dangshan pear fruit, and compared with an uninjected part and an injected no-load control GFP (Green Fluorescent Protein), the contents of stone cells and lignin are obviously increased; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, secondary cell walls of conduit cells and intervascular bundle fiber cells of the obtained PbrbHLH195 transgenic arabidopsis thaliana are remarkably thickened, accumulation of lignin in stems is increased, and the expression quantity of lignin biosynthesis related genes is remarkably increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The present invention provides a hypoxia-triggered artificial transcription factor (HATF), and further provides a hypoxia-triggered transcription control system. The transcription control system comprises a nucleic acid sequence encoding the HATF, and a recognition element (RE). The hypoxia-triggered transcription control system comprises two sets of transcription control units linked upstream and downstream, wherein the upstream transcription control unit comprises a hypoxia-triggered transcription reaction element for controlling the HATF and a nucleic acid sequence encoding the HATF, and the downstream transcription control unit comprises an RE and a gene of interest. Co-regulation by the artificial transcription factor HATF and the recognition element RE can increase the expression of the gene of interest by a factor of one hundred.

The application provides a molecular marker related to porcine reproductive and respiratory syndrome resistance in a BCL2L15 gene and application thereof, the molecular marker rs331890123 is located at a 3'UTR region of a porcine BCL2L15 gene on a 106681000th base of a chromosome 4 of a porcine reference genome. The application takes the rs331890123 as a detection site, finds that the site polymorphism significantly influences the expression of the BCL2L15 gene, and further inhibits the proliferation of a porcine reproductive and respiratory syndrome virus. The application also establishes a kind of molecular marker polymorphism Tetra-primer ARMS-PCR rapid detection method related to porcine reproductive and respiratory syndrome resistance. The application provides a new SNP molecular marker for marker-assisted selection of porcine reproductive and respiratory syndrome disease resistance breeding.

The invention discloses a method for increasing natural aroma substances of linalool, an oxide thereof and violonone of sweet osmanthus petals by applying an OfNAC72 gene, the OfNAC72 gene is an NAC transcription factor gene, an over-expression vector containing the gene OfNAC72 is transformed to the sweet osmanthus petals for transient expression, and the result shows that the linalool and the oxide thereof have the advantages that the content of linalool and the oxide thereof is increased, and the content of violonone in the sweet osmanthus petals is increased; the OfNAC72 gene expression quantity in the instantly converted sweet osmanthus petals is obviously increased, and the contents of linalool, trans-linalool oxide and various ionones (alpha-ionone, beta-ionone and dihydro-beta-ionone) in the petals are obviously increased compared with a control group; the OfNAC72 gene plays an important role in promoting synthesis of linalool and oxide thereof and ionone of the osmanthus fragrans, and can be used for improving the aroma quality of the osmanthus fragrans, breeding excellent varieties and the like in osmanthus fragrans genetic engineering.

The invention discloses an OfERF111 gene for promoting synthesis of important natural aroma substances of osmanthus fragrans linalool, trans-linalool oxide and ionone and application of the OfERF111 gene, the OfERF111 gene is an ERF transcription factor gene, an overexpression vector containing the gene OfERF111 is transformed to osmanthus fragrans petals for transient expression, and the result shows that the OfERF111 gene can be used for promoting synthesis of essential natural aroma substances of osmanthus fragrans, namely linalool, trans-linalool oxide and ionone. The OfERF111 gene expression quantity in the instantly converted sweet osmanthus petals is remarkably increased, and the contents of linalool, trans-linalool oxide and various ionones (alpha-ionone, beta-ionone and dihydro-beta-ionone) in the petals are remarkably increased compared with a control group; therefore, the OfERF111 plays an important role in promoting synthesis of linalool and oxide thereof as well as ionone, and can be used for improving the natural aroma quality of the sweet-scented osmanthus, breeding excellent varieties and the like in sweet-scented osmanthus genetic engineering.

The invention discloses a Nanguo pear PuNADP-ME gene, an overexpression and silence vector and application of the Nanguo pear PuNADP-ME gene, and belongs to the technical field of genetic engineering breeding. The nucleotide sequence of the Nanguo pear PuNADP-ME gene is SEQ ID No. 1, and the amino acid sequence coded by the gene is SEQ ID No. 2; the overexpression vector comprises a Nanguo pear PuNADP-ME gene, and is constructed by positively inserting the Nanguo pear PuNADP-ME gene into a plant expression vector pRI101-CaMV35S; the silencing vector comprises a Nanguo pear PuNADP-ME gene, and the silencing vector is constructed by reversely inserting the Nanguo pear PuNADP-ME gene into a plant expression vector pRI101-CaMV35S; according to the invention, overexpression and silent vectors are introduced into Nanguo pear fruits by using an agrobacterium-mediated transformation method, and the content of malic acid in Nanguo pears is adjusted through the overexpression vector and silent vector of the Nanguo pear PuNADP-ME gene, so that the flavor and quality of Nanguo pears are improved, and theoretical basis and technical support are provided for improving the flavor of Nanguo pears. The important application value is realized.

Plant stress resistance genes GsZIP7 In the field of plant genetic engineering technology, this invention relates to the application of salt stress. To identify genes that regulate salt stress, this invention provides a plant stress-resistance gene for regulating plant salt stress tolerance. GsZIP7 The plant stress resistance gene GsZIP7 The nucleotide sequence is shown in SEQ ID NO.1, and a recombinant vector and recombinant bacteria containing the above gene were constructed to obtain transgenic plants; the plant stress resistance gene GsZIP7 Positive regulation of a plant's ability to tolerate salt stress can lay the foundation for research into breeding salt-tolerant transgenic plants.

The application discloses a broad-spectrum disease-resistant gene Rpl-avh23 of Nicotiana plumbaginifolia, and an encoding protein and application thereof. The application separates a disease-resistant gene Rpl-avh23 from the Nicotiana plumbaginifolia, the full-length cDNA of the gene is 2154 bp, the nucleotide sequence is shown as SEQ ID NO:1; the protein encoded by the gene is an intracellular immune receptor protein with a full length of 717 amino acids, and the amino acid sequence is shown as SEQ ID NO:2. The application finds that the Rpl-avh23 gene can improve the resistance of plants to oomycetes, fungi and / or viruses through transcription mode analysis and plant expression analysis, and therefore, the disease-resistant gene Rpl-avh23 can be applied to improving the disease resistance of plants or plant breeding.

The present invention relates to a method for constructing an atopic dermatitis skin organoid from human pluripotent stem cells. It was observed that ALI-skin organoids of the present invention formed skin cells, appendages, neural cells, adipocytes, and the like in similar structures to those in the real skin and exhibited greatly improved follicle growth, compared to skin organoids constructed by existing culture methods. In addition, as a result of treatment with Staphylococcus aureus to construct an atopic dermatitis model, a good simulation was made of the characteristics of atopic dermatitis, such as damaged skin barriers, increased epithelium-derived cytokines and downregulated epidermal stem cell expression, causing Staphylococcus aureus colonies, and the like. Thus, the skin organoid of the present invention is expected to find advantageous applications as a real skin model and an atopic dermatitis model.

The invention discloses a construction method and application of a recombinant engineering bacterium with an antibacterial immune peptide Scy displayed on the surface. According to the present invention, the probiotic Escherichia coli Niss1917 (EcN) is adopted as the host bacterial strain, and the pnirBmisL (pnir) is adopted as the plasmid vector, such that the efficient display of the antibacterial immune peptide Scy in the crab semen from the heterologous source on the host bacterial surface is successfully achieved; according to the method, a complex purification step of the antibacterial peptide is avoided, so that the antibacterial peptide Scy can be directly applied through the engineering bacterium EcN-Scy. The engineering bacteria still show relatively high survival activity under the condition of simulating strong acid and high salt of gastrointestinal tracts; meanwhile, the composition has a protection effect on mouse intestinal mucosal barrier injury caused by dextran sodium sulfate (DSS), can remarkably relieve DSS-induced mouse weight loss, shortened colon length and other symptoms, improves the colon form, relieves liver injury, and can be used for preventing and treating liver injury through multiple ways of regulating intestinal immune cell factors, enhancing the oxidation resistance, maintaining intestinal physical barriers and the like. The intestinal health is comprehensively protected, and a new biological preparation candidate is provided for treating the inflammatory bowel disease.