58 results about "IMPACT gene" patented technology

This invention is in the area of cobalamin-mediated delivery of nucleic acids and analogs and derivatives thereof to a host to affect gene expression.

Embodiments of the present invention include novel inference methods to analyze complex biological information, including gene networks. In some embodiments, time course data is obtained simultaneously for a large number of genes in an organism. New methods include refinements of Bayesian inference methods and the application of those methods to determine causal relationships between expressed genes and, in some embodiments, to determine upstream influencing genes of regulated genes. Additional refinements of the Bayesian model include the use of bootstrapping methods and determination of marginal effects, thereby providing more accurate information on the network between expressed genes. Information about the gene network can be stored in a memory device and sent to an output device or to a remote location.

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and / or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

The invention discloses a method for quickly detecting RFLP (restriction fragment length polymorphism) of SNP (single nucleotide polymorphism) of a common ox SIRT1 (silent information regulator 1) gene promotor region. The method comprises the following steps of: taking whole genome DNA (deoxyribonucleic acid) of common ox blood as a template and a primer pair P (F and R) as a primer; performing PCR (polymerase chain reaction) amplification on the common ox SIRT1 gene promotor region; digesting a PCR amplification product by using restriction endonuclease SmaI; performing agarose gel electrophoresis on segments after enzyme digestion; and identifying the SNP of the common ox SIRT1 promotor region according to a result of the agarose gel electrophoresis. Because important functions including muscle differentiation, lipogenesis and the like related to the SIRT1 are closely related to common ox meat quality characteristics, and the SNP can significantly affect the promotor activity of the SIRT1 gene and is related to various important economic characteristics of the common ox, the detection method provided by the invention lays a foundation for the establishment of the relationship between the SNP and growth characteristics of the SIRT1 gene, and can be used for marker assisted selection of the growth characteristics of the Chinese common ox so as to facilitate rapid establishment of common ox populations with excellent genetic resources.

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the transcription rate and / or expression rate of genes, expression cassettes containing said expression units, genetically modified microorganisms with a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating the genetically modified microorganisms.

Five microRNA genes adopting a populus cellulose synthase gene as a target gene and applications thereof are provided. The micRNAs have sequences shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5. The miRNAs have specific expression parts in plants and influence synthesis of cellulose in the specific parts of the plants. After precursor genes of the miRNAs, or mature miRNAs are directly put into expression vectors and transformed into the plants, precursors with occipital ring structures are formed in the plants, and is processed and modified to form mature miRNAs, thus inhibiting or influencing gene expression and achieving an objective of gene modification.

The invention discloses a gene mutation pathogenicity detection method and system based on a neural network and a medium. The method comprises the steps of inputting a gene detection VCF file to be detected and an HPO phenotype; obtaining a characteristic value of each gene variation according to the to-be-detected gene detection VCF file and the HPO phenotype; for each gene variation, inputting the characteristic value of the gene variation into a trained neural network model to obtain a pathogenicity comprehensive analysis result of the gene variation, wherein the neural network model is pre-trained to establish a mapping relationship between the characteristic value of each gene variation and the pathogenicity comprehensive analysis result of each gene variation. According to the invention, the subjective defect of manual analysis is overcome, and various factors influencing the pathogenicity of gene mutation can be comprehensively considered, so that the comprehensive analysis result is more objective and effective, the accuracy of pathogenicity analysis of gene mutation is greatly improved, and the gene interpretation efficiency is improved.

The invention discloses an interpretable deep learning-based innate immune gene expression prediction method, which comprises the following steps of: 1, collecting and preprocessing a DNA (Deoxyribose Nucleic Acid) promoter sequence and epigenetic modification data, and dividing a preprocessed data set into a training set and an independent test set so as to verify the prediction performance of a model; 2, constructing a human innate immune gene expression prediction network based on residual connection, a convolutional neural network and an attention mechanism; 3, performing model training on a plurality of cell lines to obtain an optimal human innate immune gene expression prediction model, and evaluating the prediction effect of the model on the independent test set; and 4, carrying out post interpretability analysis on the model through an integral gradient method, and identifying a key motif which is most likely to influence gene expression. According to the method, DNA sequence information and epigenetic modification information are extracted more effectively, and a new tool is provided for deeply understanding a regulation mechanism of an innate immune gene and developing individualized medical treatment.

The invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units therefor, methods for modifying or generating the transcription rate and / or expression rate of genes, expression cassettes containing the expression units, genetically modified micro-organisms with modified or generated transcription rates and / or expression rates, and methods for producing biosynthetic products by cultivating the genetically modified micro-organisms.

The invention provides a separate sgRNA molecule capable of specifically acting on a target site, and a method of acquiring the sgRNA molecule. The invention also provides a method of site-directed gene modification by using the specific sgRNA molecule. The sgRNA molecule can specifically act on the target site without affecting other sites having high sequence homology in genome.

The invention belongs to the technical field of gene engineering, and discloses sgRNA for pedigree tracing of a targeted Rosa26 gene, the sgRNA comprises sgRNA1, sgRNA2, sgRNA5, sgRNA6 and sgRNA7, and the nucleotide sequences of corresponding DNA double strands of the sgRNA1, the sgRNA2, the sgRNA5, the sgRNA6 and the sgRNA7 are sequentially shown as SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5-6, SEQ ID NO: 7-8 and SEQ ID NO: 9-10. The sgRNA can target a mouse Rosa26 gene, is combined with a CRISPR / Cas9 targeting vector, and can cut different sites of the Rosa26 gene to generate different bar codes, the diversity of the obtained bar codes can be used for reconstructing a cell lineage and tracing the cell lineage, meanwhile, the transcription of the Rosa26 gene is not influenced after cutting, and the cell lineage relationship can be comprehensively tracked in a complex cell system.

The invention relates to the technical field of molecular biology and gene engineering, and particularly discloses siRNA and an application thereof. The siRNA inhibits IHH gene expression, the siRNA is a double strand, and the nucleotide sequence of the siRNA is as follows: a positive-sense strand: 5 'CCGACAUCAUCUUCAAGGATT 3'; and an antisense strand: 5 'UCCUUGAAGAUGAUGUCGGTT 3'. According to the invention, the siRNA influences the IHH gene function and the expression level of the signal channel gene so as to influence the creeping character of the chicken, so that people can deeply understand the molecular mechanism and the regulation mechanism formed by the creeping character of the chicken, and breed conservation and improvement work of the creeping chicken can be further carried out; and the application plays an important role in molecular breeding and production of important economic characters of livestock and poultry.

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and / or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

The invention relates to the technical field of gene prediction, and particularly discloses a functional gene prediction method based on deep learning, which comprises the following steps of: 1, detecting physiological characteristics of a target individual so as to judge whether a target functional gene is expressed or not and judge the expression degree; 2, analyzing the relationship between the environment and the expression of the target functional gene so as to judge whether the change of the environment has an influence on the expression of the target functional gene or not; by analyzing the relationship between the environment and the expression of the target functional gene, how the environmental factors influence the expression of the gene can be revealed, so that the interaction mechanism of the gene and the environment is deeply understood, a corresponding prevention strategy is formulated, the expression of the gene is regulated and controlled by changing the environmental factors, the accuracy of gene mining and identification is improved, and the application prospect is wide. And a powerful tool is provided for further deeply researching the action mechanism of the gene function.

The invention provides a kit and buffer solution box integrated device for a gene sequencer and the gene sequencer, and relates to the technical field of gene sequencing, the kit and buffer solution box integrated device comprises a kit and a buffer solution box, and a box body of the kit is provided with a buffer solution box accommodating part; a detachable first identification piece is arranged on the side, close to the containing part, in the kit, the first identification piece is provided with a first identification part, and the first identification part comprises a plurality of first identification areas used for identifying information of the kit; the buffer solution box is provided with a detachable second identification piece, the second identification piece is provided with a second identification part, and the second identification part comprises a plurality of second identification areas used for identifying information of the buffer solution box; a first fool-proof part and a second fool-proof part are respectively arranged on the first identification piece and the second identification piece; the first fool-proof part and the second fool-proof part are matched with each other, so that the buffer solution box is matched with the kit. The technical problem that in the prior art, an independent kit and a buffer solution box affect the overall size of the gene sequencer is solved.

The invention discloses a gene combination for ASD evaluation and application thereof, and belongs to the technical field of gene detection. The gene combination for ASD evaluation is obtained on the basis that 1089 ASD cases are subjected to whole exome sequencing, the genetic structure of ASD is comprehensively analyzed, harmful variation in ASD related genes is comprehensively analyzed, 168 high-influence genes are obtained through comprehensive analysis and evaluation, the ASD related genes recorded in a part of a database are determined as low-influence genes, and the ASD related genes recorded in a part of a database are determined as low-influence genes. The detection rate of harmful variation of the high-influence gene combination in an ASD case group reaches 21.12%, which indicates that the high-influence gene combination can assist in improving the accuracy of ASD clinical diagnosis based on a genome level.

The invention provides a suicide-homicide plasmid, a saccharomyces cerevisiae traceless gene editing method using the suicide-homicide plasmid and application. The suicide-homicide plasmid comprises areplication origin, a selection marker, a Cas9 protein expression cassette and a sgRNA1 expression cassette, wherein the sgRNA1 expression cassette comprises a promoter, sgRNA1, scaffold and a termination sequence; and the sgRNA1, the scaffold and the termination sequence are designed for a common sequence of a gene editing vector and the suicide-homicide plasmid. The gene editing vector is transferred into saccharomyces cerevisiae, and then the suicide-homicide plasmid is transferred into the saccharomyces cerevisiae, so that a traceless edited saccharomyces cerevisiae strain can be obtained. The saccharomyces cerevisiae is edited by using the suicide-homicide plasmid, so that selection of the strain which successfully transforms the gene editing plasmid after gene editing is not affected, and the plasmid eliminating success rate is remarkably increased; and meanwhile, the traceless editing effect can be achieved through two rounds of plasmid transformation, and the traceless editingrate of the saccharomyces cerevisiae is further increased.

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and / or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

The invention discloses a neural network-based gene mutation pathogenicity detection method, system and medium. The method of the invention includes inputting a gene to be detected to detect a VCF file and an HPO phenotype; detecting a VCF file and an HPO table according to a gene to be detected. type to obtain the eigenvalues ​​of each gene variation; for each gene variation, input the eigenvalues ​​of the gene variation into the trained neural network model to obtain the comprehensive analysis results of the pathogenicity of the gene variation, the neural network model is pre-trained and established The mapping relationship between the eigenvalues ​​of each gene variation and the comprehensive pathogenicity analysis results of each gene variation. The present invention not only overcomes the subjectivity defect of manual analysis, but also can comprehensively consider various factors affecting the pathogenicity of gene mutation, so that the result of comprehensive analysis is more objective and effective, and greatly improves the accuracy of analysis on the pathogenicity of gene mutation , improving the efficiency of gene interpretation.

The invention provides a molecular marker related to porcine reproductive and respiratory syndrome resistance in a BCL2L15 gene and application of the molecular marker. The molecular marker rs331890123 is located at the 106681000th basic group of a chromosome 4 of a pig reference genome and is located in a 3 'UTR (Untranslated Region) region of the pig BCL2L15 gene. According to the invention, rs331890123 is taken as a detection site, and the polymorphism of the site obviously affects the expression of the BCL2L15 gene, so that the proliferation of the porcine reproductive and respiratory syndrome virus is inhibited. The invention also establishes a rapid detection method of the molecular marker polymorphism Tetra-primer ARMS-PCR (Reverse Transcription-Respiratory Syndrome-Polymerase Chain Reaction) related to the resistance of the porcine reproductive and respiratory syndrome. The invention provides a novel SNP molecular marker for marker-assisted selection of porcine reproductive and respiratory syndrome resistance breeding.

The invention belongs to the technical field of biological breeding, and discloses a genome selection method based on a population differentiation index FST. As the GBLUP method assumes that the LDs among the populations are consistent, and the genomic selection accuracy is influenced by directly utilizing the GBLUP method, compared with a GBLUP model, the model fstGBLUP provided by the invention can consider the LD inconsistency of the QTL and the SNP among the populations, so that the genomic selection accuracy is improved. The additive genome genetic relationship matrix constructed by the SNP screened by the group differentiation index FST and the additive genome genetic relationship matrix constructed by the remaining SNPs are respectively established, so that the method has higher predictive power, namely higher genome selection accuracy.

The invention provides a method for regulating and controlling the transcriptional activity of a PAFAH1B1 gene and application of the method, and belongs to the technical field of gene expression regulation and control. The method comprises the step of influencing the methylation level of a PAFAH1B1 gene promoter region by using a gRNA fragment, a DNA methyltransferase inhibitor or an RNA interference fragment of DNA methyltransferase. The method is applied to the ovarian granulosa cells, and under different treatments, the mRNA level and the protein expression level of PAFAH1B1 in the ovarian granulosa cells can be remarkably promoted or inhibited. According to the application, the obvious influence of DNA methylation on the transcriptional activity of the PAFAH1B1 gene in the ovarian granular cells is determined for the first time, the position of the DNA methylation applied to the promoter region of the PAFAH1B1 gene is determined for the first time, and the mechanism that the DNA methylation affects the expression of the PAFAH1B1 gene by changing the chromatin structure of the promoter region of the PAFAH1B1 gene is disclosed, so that the expression of the PAFAH1B1 gene in the ovarian granular cells is improved. The invention provides a simpler and more effective new way for regulating and controlling granular cell functions and ovarian development, and has important application value for subsequent research on mammal ovarian granular cell functions, follicular development and initial estrus initiation.

The invention relates to the technical field of pancreatic cancer SNP sites. The invention provides an SNP (Single Nucleotide Polymorphism) site related to pancreatic cancer susceptibility and application of the SNP site. The SNP site is rs11102484. A genetic variation (eQTL) map influencing gene expression is systematically drawn in a whole genome range by integrating multiple omics data such as a genome and a transcriptome, 82 potential pathogenic sites are further identified in combination with pancreatic cancer GWAS data of multiple people, and the expression abundance of 15 functional genes is regulated and controlled. Wherein the genetic variation with the highest comprehensive function score is rs11102484 located in a chromosome 1p13.2 region, and multi-population case contrast research proves that the mutation of the locus C > G can significantly reduce the onset risk of pancreatic cancer. The invention discloses a new mechanism of pancreatic cancer genetic susceptibility, deepens understanding of pancreatic cancer pathogenesis, and provides a certain scientific basis for individualized prevention of pancreatic cancer.

Methods for inactivating in a cell a mutant allele of the elastase, neutrophil expressed gene (ELANE gene) gene having a mutation associated with severe congenital neutropenia (SCN) or cyclic neutropenia (CyN) and which cell is heterozygous at one or more polymorphic sites selected from the group consisting of: rs10424470, rs4807932, rs10414837, rs376107533, rs3761010, rs10413889, rs3761007, rs10409474, rs3761005, rs351107, rs3761001, rs740021, rs781452480, rs371057361, rs570466264, rs1041904080, rs7250194, rs17216649, rs199720952, rs6510983, rs17223066, rs7255385, rs112639467, rs141213775, rs28591229, rs10469327, rs3834645, rs1683564, rs71335276, and rs8107095, the method comprisingintroducing to the cell a composition comprising:a CRISPR nuclease or a sequence encoding the CRISPR nuclease; anda first RNA molecule comprising a guide sequence portion having 17-30 nucleotides,wherein a complex of the CRISPR nuclease and the first RNA molecule affects a double strand break in the mutant allele of the ELANE genethe method optionally further comprising introduction of a second RNA molecule comprising a guide sequence portion capable of complexing with a CRISPR nuclease, wherein the complex of the second RNA molecule and CRISPR nuclease affects a second double strand break in the ELANE gene.

The invention discloses a method for constructing a gene regulatory network by utilizing eQTL and application, and the method specifically comprises the following steps: utilizing eQTL data, calculating linkage unbalance degree between eSNP in each eQTL and all other SNPs in a 1 million base pair range of a chromosome where the eSNP is located through an eQTL2GRN program, classifying according to the linkage unbalance degree, and utilizing physical coordinate positions of the eSNP and eGene to construct a gene regulatory network. Judging that the regulation and control type is cis or trans; and finally, constructing a gene regulation and control network, and carrying out visualization. By means of the method, the regulation and control relation between the genes can be recognized and analyzed, and then how genetic variation affects gene expression and phenotypic characteristics can be deeply understood.

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and / or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for influencing 49-day-old chicken body weight by influencing GCNT7 gene expression and application thereof, and belongs to the fields of molecular genetics and agricultural animal breeding, the SNP molecular marker is located at the position of 12292590bp of a chicken 20 # chromosome, a reference genome version is GRCg7b, a basic group at the position has A / G polymorphism, and the SNP molecular marker can be used for identifying the chicken 49-day-old body weight. The individual with the genotype of GG or GA at the position has a higher 49-day-old body weight character than the individual with the genotype of AA. A molecular marker system which is remarkably related to the GCNT7 expression level and can be used for early prediction and selection of the 49-day-old chicken weight character is constructed, and a feasible technical path is provided for broiler chicken breeding practice.

This invention provides a method for rapidly identifying exogenous inserted sequences based on whole-genome sequencing data, comprising the following steps: sequencing data quality control, preliminary data alignment, screening of preliminary alignment results, evaluation of secondary alignment results, homology detection, preliminary insertion region detection, precise insertion site identification, assembly of inserted and flanking sequences, acquisition of genes affected by the insertion site, and primer design for the insertion site. Compared with traditional experimental detection techniques, this invention is less time-consuming, reproducible, and presents more comprehensive transgenic insertion characteristics; compared with other existing whole-genome resequencing methods, it is more accurate and intuitive, the results are easier to understand, and it is more conducive to the safety risk assessment of transgenic plants and animals, thus contributing to the development and application of transgenic or gene editing technologies.

The invention discloses a method for influencing the activity of gene telomerase through serum protein bases. The method comprises the following steps: step 1, splitting a to-be-detected cell, carrying out centrifuging and separating to obtain a telomerase-containing supernatant; 2, mixing the telomerase-containing supernatant with a telomerase substrate to generate a telomerase extension product;3, after the telomerase substrate extension reaction is completed, carrying out amplification reaction, and finally, forming a fluorescence detection signal; and 4, detecting the intensity of the fluorescence signal by using a luminoscope. According to the method for influencing the activity of the gene telomerase through the serum protein bases provided by the embodiment of the invention, the oligonucleotide single strand can be rapidly amplified under an isothermal condition by virtue of the extension reaction of the DNA polymerase with strand displacement activity and the property of recognizing and cutting the single strand in the specific double strand by virtue of the nucleases. The reaction not only has high amplification efficiency and can realize real-time and rapid detection, but also does not need accurate thermal cycle equipment.