siRNA and application thereof

A gene expression and nucleic acid sequence technology, applied in the fields of molecular biology and genetic engineering, can solve problems that need to be further studied

Pending Publication Date: 2021-10-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prostrate trait is controlled by the dominant homozygous lethal gene Cp on the autosome (Landauer W, et al., 1930), but international research on its traits and genes still needs to be in-depth

Method used

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  • siRNA and application thereof
  • siRNA and application thereof
  • siRNA and application thereof

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Embodiment Construction

[0019] The application will be described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific embodiments are only used to further illustrate the application, and cannot be interpreted as limiting the protection scope of the application. The above application content makes some non-essential improvements and adjustments to this application.

[0020] 1. Materials

[0021] The methods used in the present embodiment are conventional methods known to those skilled in the art unless otherwise specified, and materials such as reagents used, if not otherwise specified, are commercially available products.

[0022] 2. Method

[0023] 2.1 siRNA sequence design

[0024] Obtain the IHH gene sequence (NM_204957.2) from GeneBank according to bioinformatics methods, as shown in SEQ ID NO.1, and use the relevant biological website (http: / / sidirect2.rnai.jp / ; http: / / sidirect2.rnai.jp / ; http: / / biodev.extra.cea.fr / DS...

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Abstract

The invention relates to the technical field of molecular biology and gene engineering, and particularly discloses siRNA and an application thereof. The siRNA inhibits IHH gene expression, the siRNA is a double strand, and the nucleotide sequence of the siRNA is as follows: a positive-sense strand: 5 'CCGACAUCAUCUUCAAGGATT 3'; and an antisense strand: 5 'UCCUUGAAGAUGAUGUCGGTT 3'. According to the invention, the siRNA influences the IHH gene function and the expression level of the signal channel gene so as to influence the creeping character of the chicken, so that people can deeply understand the molecular mechanism and the regulation mechanism formed by the creeping character of the chicken, and breed conservation and improvement work of the creeping chicken can be further carried out; and the application plays an important role in molecular breeding and production of important economic characters of livestock and poultry.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and specifically relates to siRNA and its application. Background technique [0002] The prostrate character of chicken is a unique dwarf phenotype in some chicken breeds, and it is a unique limb abnormal phenotype in chickens. The creeping trait is a classic trait in genetics. Individuals show short shins, short wings, well-proportioned body, abnormal bone development, especially tibial cartilage dysplasia. The prostrate character is controlled by the dominant homozygous lethal gene Cp on the autosome (Landauer W, et al., 1930), but the research on its character and gene in the world still needs to be deepened. [0003] The Indian hedgehog gene (IHH) encodes a member of the hedgehog family of signaling factors and is initially expressed in chondrocytes of early cartilaginous skeletal elements (Bitgood MJ, et al., 1995). IHH is the earliest discovered importan...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/6888
CPCC12N15/113C12Q1/6888C12N2310/14C12Q2600/124C12Q2600/158C12Q2600/178
Inventor金四华何培莉税斐耿照玉贾羽晴夏晶晶高伟凤郑书丽江洪峰郭立平
OwnerANHUI AGRICULTURAL UNIVERSITY