Pef-ts expression units

A nucleic acid and nucleotide technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, peptide peptides, etc., and can solve the problems of enhanced expression of undisplayed genes

Inactive Publication Date: 2007-06-27
BASF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, transformed cells grown on glucose did not show enhanced expression of this reporter gene

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach b1

[0157] According to embodiment b1), one or more nucleic acids of the present invention having promoter activity and altered specific promoter activity under appropriate conditions are introduced into the microbial genome, so as to have the introduced promoter activity under appropriate conditions. The transcription of one or more endogenous genes is carried out under the control of a nucleic acid with an altered specific promoter activity, or

Embodiment approach b2

[0158]According to embodiment b2), one or more endogenous genes are introduced into the microbial genome, so that one or more endogenous genes are introduced under the control of the endogenous nucleic acid of the present invention with promoter activity and altered specific promoter activity under appropriate conditions. Transcription of multiple imported endogenous genes, or

Embodiment approach b3

[0159] According to embodiment b3), one or more nucleic acid constructs are introduced into microorganisms, the nucleic acid constructs comprising the nucleic acid of the present invention having promoter activity, altered specific promoter activity under appropriate conditions, and functionally linked One or more endogenous nucleic acids to be transcribed.

[0160] Therefore, in addition, it is possible to influence the transcription rate of foreign genes compared with wild-type in the following ways:

[0161] According to embodiment b2), one or more exogenous genes are introduced into the microbial genome, so that one or more exogenous genes are introduced under the control of the endogenous nucleic acid of the present invention with promoter activity and altered specific promoter activity under appropriate conditions. Transcription of multiple imported foreign genes, or

[0162] According to embodiment b3), one or more nucleic acid constructs are introduced into microorganisms,...

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Abstract

The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the transcription rate and / or expression rate of genes, expression cassettes containing said expression units, genetically modified microorganisms with a modified or induced transcription rate and / or expression rate, and methods for producing biosynthetic products by cultivating the genetically modified microorganisms.

Description

Technical field [0001] The present invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, new promoters and expression units thereof, methods for changing or influencing gene transcription and / or expression rates, expression cassettes containing the expression units, and modified Or a genetically modified microorganism whose transcription rate and / or expression rate are affected and a method for preparing biosynthetic products by cultivating the genetically modified microorganism. Background technique [0002] A variety of biosynthetic products, such as fine chemicals (especially amino acids, vitamins) and proteins are produced in cells through natural metabolic processes, and are used in many industries including food, feed, cosmetics, feed, food, and medicine. In the industrial field. These substances are collectively referred to as fine chemicals / proteins, which especially include organic acids, proteinogenic and non-proteinoge...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/68C07K14/34
CPCC07K14/34C12P13/04C12N15/63C12N15/77C12Q1/6876
InventorO·策尔德尔C·克洛普罗格B·克勒格尔H·施罗德S·哈夫纳
OwnerBASF AG