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Pef-ts expression units comprising corynebacterium glutamicum

A nucleic acid and nucleotide technology, applied in the direction of bacteria, microbe-based methods, microbe determination/testing, etc., can solve the problem of enhanced expression of undisplayed genes

Inactive Publication Date: 2008-07-30
BASF SE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, transformed cells grown on glucose did not show enhanced expression of this reporter gene

Method used

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  • Pef-ts expression units comprising corynebacterium glutamicum
  • Pef-ts expression units comprising corynebacterium glutamicum
  • Pef-ts expression units comprising corynebacterium glutamicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach b1

[0158]According to embodiment b1), one or more nucleic acids of the present invention having promoter activity and altered specific promoter activity under appropriate conditions are introduced into the genome of the microorganism so that the introduced promoter activity under appropriate conditions has transcription of one or more endogenous genes under the control of a nucleic acid with altered specific promoter activity, or

Embodiment approach b2

[0159] According to embodiment b2), one or more endogenous genes are introduced into the microorganism genome so that one or more endogenous genes can be carried out under the control of the endogenous nucleic acid of the present invention having promoter activity, under appropriate conditions, with altered specific promoter activity. Transcription of multiple introduced endogenous genes, or

Embodiment approach b3

[0160] According to embodiment b3), one or more nucleic acid constructs comprising a nucleic acid according to the invention with promoter activity, under appropriate conditions with altered specific promoter activity, and a functionally linked One or more endogenous nucleic acids to be transcribed.

[0161] Therefore, it is additionally possible to affect the transcription rate of the exogenous gene compared to the wild type by:

[0162] According to embodiment b2), one or more exogenous genes are introduced into the microorganism genome, so that one or more genes can be carried out under the control of the endogenous nucleic acid of the present invention having promoter activity, under appropriate conditions, with altered specific promoter activity. Transcription of multiple introduced exogenous genes, or

[0163] According to embodiment b3), one or more nucleic acid constructs comprising a nucleic acid according to the invention with promoter activity, under appropriate co...

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Abstract

A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).

Description

[0001] This application is filed by the inventor on July 16, 2005 and is entitled "Containing P EF-TS A divisional application of the Chinese patent application 200580024140.1 for the expression unit of Corynebacterium glutamicum. technical field [0002] The present invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, new promoters and expression units thereof, methods for changing or influencing gene transcription rate and / or expression rate, expression cassettes comprising the expression unit, Genetically modified microorganisms with affected transcription rate and / or expression rate and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms. Background technique [0003] A variety of biosynthetic products such as fine chemicals (such as amino acids, vitamins among others) and proteins are produced in cells through natural metabolic processes and are used in many industries inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/77C12N1/21C12P13/08C12P13/12C12R1/15C12R1/13
CPCC07K14/34C12P13/04C12N15/63C12N15/77C12Q1/6876
Inventor O·策尔德尔C·克洛普罗格B·克勒格尔H·施罗德S·哈夫纳
Owner BASF SE