64 results about "Genetically modified microorganisms" patented technology

The invention provides a method for producing borneol, the method comprising culturing the transgenic microorganism in a culture medium, and the microorganism comprises or expresses borneol diphosphate synthetase and phosphatase.

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.

Disclosed herein are genetically engineered Kluyveromyces sp. cells capable of producing D-allulose. The genetically engineered Kluyveromyces sp. cells comprise an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The invention includes novel routes of synthesis that involve the use of an acyloin condensation reaction between a ketone and formyl-CoA to form a corresponding branched 2-hydroxyacyl-CoAs and forming branched chain products therefrom. Described are genetically modified microorganisms that produce a branched product from a ketone and formyl-CoA and methods of producing a branched product, including in vivo and in vitro methods.

Disclosed herein are genetically engineered Kluyveromyces marxianus cells capable of producing D-allulose with increased talitol formation. The engineered cell may comprise a genetic modification resulting in overexpression of a native talitol dehydrogenase enzyme; an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

The present description relates to a transgenic microorganism capable of converting vegetable oils into olefins. The present description also discloses a process for obtaining such hydrocarbons from triglycerides or free fatty acids of plant origin, which is carried out by the microorganism disclosed in the present description.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Methods for producing organosulfur compounds from a prokaryotic cell comprising enzymes involved in the production of cysteamine, hypotaurine, and taurine. Said prokaryote may comprise a vanin selected from vanin-1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and / or a flavin-containing monooxygenase 1 (fmo1). Methods include the expression of organosulfur compounds in Corynebacterium glutamicum with incubation conditions to promote compound production.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

The purpose of the present disclosure is to provide: a method for producing β-carotene and a β-carotene derivative; and a genetically modified microorganism. The present disclosure provides: a method for producing β-carotene and / or a β-carotene derivative using a genetically modified microorganism that belongs to the genus Lipomyces; and a genetically modified microorganism that belongs to the genus Lipomyces, and that has a β-carotene biosynthesis pathway and produces β-carotene and / or a β-carotene derivative through fermentation.

The present invention relates to an enzyme cocktail, a method for the deconstruction of lignocellulosic biomass using said cocktail, and a genetically modified microorganism for producing heterologous enzyme aimed at making biomass fermentable into biorenewable products such as biofuels, organic acids, and hydrocarbon.

A genetically modified microorganism that can produce 3-hydroxyadipic acid and / or α-hydromuconic acid with a high yield; and a method of producing 3-hydroxyadipic acid and / or α-hydromuconic acid using the genetically modified microorganism, are disclosed. The genetically modified microorganism has an ability to produce 3-hydroxyadipic acid and / or α-hydromuconic acid, and has an enhanced enzymatic activity to catalyze a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, wherein, in the genetically modified microorganism, a dicarboxylic acid excretion carrier function is deleted or decreased.

Among the various aspects of the present disclosure is the provision of a biological and biochemical production of piperazic acid derived from the newly discovered production pathway for L-piperazic acid. One aspect of the present disclosure includes a transgenic microorganism (e.g., bacteria) engineered to accumulate piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes biochemical and biological methods for producing piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes compositions and methods of using isotopically labeled piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product.

The invention belongs to the food industry, relates to a novel method for producing casein compositions by culturing transgenic microorganisms in the presence of whey, and limits the environmental impact.

The present invention provides a method for producing liquid methionine or methionine, and particularly provides a method for producing liquid methionine or methionine by condensation reaction of 3-hydroxypropionaldehyde and formyl-CoA derived from a C1 substrate. The present invention also provides a genetically engineered microorganism for the production of liquid methionine or methionine.

Disclosed herein are genetically engineered Kluyveromyces marxianus cells capable of producing D-allulose. The genetically engineered Kluyveromyces marxianus cells comprise a deletion or disruption in a native PFK1 gene; an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:192, 194, and 196, and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

A process (1) for producing an article (2) from a plant-based material (3) includes providing (4) genetically modified microorganisms (5) in which the genetic modification (12) results in overexpression of at least one adhesive protein (6); mixing (7), preferably blending, of the plant-based material (3) with the genetically modified microorganisms (5), which are preferably held in a culture medium (11), to obtain a mixture (8); expressing of the adhesive protein (6); and hardening (10) of the mixture (8).

A genetically modified microorganism that contains a gene capable of expressing a variant YeeX protein or a homolog thereof, said variant protein having an amino acid sequence derived from the amino acid sequence of a wild type YeeX protein or a homolog thereof by substitution, insertion and / or deletion of one to several amino acids. This genetically modified microorganism has an improved organic acid productivity compared to the microorganism before the genetic modification.

The present disclosure relates to genetically modified microorganisms for improving the phenotype of a plant, such as the nitrogen effectiveness of a leguminous plant. The present disclosure includes novel strains of microbial bodies, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important species, such as fixation, utilization, regulation, absorption, acquisition, tolerance and / or processing of nitrogen in plants.

Disclosed herein are genetically engineered yeast cells and genetically engineered bacterial cells capable of producing D-allulose. The genetically engineered cells comprise an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:192, 194, and 196, and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

A method for producing isoprene glycol, including: enzymatically generating 3-hydroxy-3-methylbutyryl-CoA (HMB-CoA) from acetyl-CoA (Ac-CoA); enzymatically generating 3-methyl-3-hydroxybutyrylaldehyde (3-HMBA) from HMB-CoA; and enzymatically converting 3-HMBA to isoprene glycol (ISPG). Wherein the enzymatically generating 3-HMBA from HMB-CoA includes: enzymatically reducing HMB-CoA to produce 3-HMB; or enzymatically hydrolyzing HMB-CoA to 3-hydroxy-3-methylbutyric acid (HMB) and then enzymatically reducing HMB to 3-HMBA. Also provided herein are an enzyme composition, a nucleic acid composition, and a transgenic microorganism for producing isoprene glycol.

The present invention encompasses a novel synthetic route involving the use of an acyloin condensation reaction between a ketone and formyl-CoA to produce the corresponding branched 2-hydroxyacetyl-CoA, and thereby a branched product. Also described are genetically modified microorganisms for producing branched chain products from ketones and formyl-CoA and methods of producing branched chain products, including in vivo and in vitro methods.

This invention aims to develop microorganisms that can produce 3 HP more efficiently. [Solution] A genetically modified microorganism having the ability to produce 3-hydroxypropionic acid, comprising the following gene: (i) the gene encoding acryloyl-CoA reductase, and (ii) gene encoding crotonase The mutation includes, wherein the mutation results in the suppression of the expression of the genes (i) and (ii) above, or inactivation of the enzymes shown in (i) and (ii) above, or a reduction in enzyme activity compared to a control without the mutation. Furthermore, a microorganism into which a gene encoding malonyl-CoA reductase has been introduced.

This disclosure relates to genetically modified microorganisms for improving plant phenotypes, such as nitrogen use efficiency in non-leguminous plants. It includes novel strains of microorganisms, microbial aggregates, and agricultural compositions comprising them. Furthermore, this disclosure teaches methods for utilizing the described microorganisms, the microbial aggregates, and the agricultural compositions comprising them in methods for conferring beneficial properties to target plant species. In a particular aspect, this disclosure provides methods for enhancing desired plant traits of agronomically important species, such as nitrogen fixation, utilization, regulation, absorption, acquisition, tolerance, and / or treatment in plants.

Provided is a genetically modified microorganism that has enhanced GlcNAc-β1,6 transferase activity and can be used to produce a branched HMO. The genetically modified microorganism has enhanced activity with respect to a protein represented by at least one of [1]–[3] and produces a sugar that has a GlcNAc-β1,6-Gal structure. [1] A protein that includes an amino acid sequence represented by one of SEQ ID NO:47–53 and 55–60. [2] A protein that: includes an amino acid sequence that results from the deletion, substitution, insertion, or addition of 1–40 amino acids to an amino acid sequence represented by one of SEQ ID NO:47–53 and 55–60; and has GlcNAc-β1,6 transferase activity with respect to a receptor substrate that includes galactose. [3] A protein that: includes an amino acid sequence that has at least 50% identity with an amino acid sequence represented by one of SEQ ID NO:47–53 and 55–60; and has GlcNAc-β1,6 transferase activity with respect to a receptor substrate that includes galactose.

The disclosure relates to novel methods, compositions, and genetically modified microorganisms for extracting and / or isolating bioproducts from microorganisms having recalcitrant cell walls. In some aspects, the disclosure relates to solvent-free methods of extracting and / or isolating bioproducts. The disclosure further relates to bioproducts having less than 10 ppm of a solvent.

Disclosed are a genetically modified microorganism and the use thereof. The genetically modified microorganism expresses at least one, two or three exogenous genes (AMUC_1100 polypeptide, IL-10 and / or IL-22). The genetically modified microorganism can still stably survive after integrating exogenous target genes therein; and efficiently expresses and secretes proteins or polypeptides encoded by the exogenous genes in the intestinal tract, and the expressed and secreted proteins or polypeptide encoded by the exogenous genes can still have good activity. Therefore, the genetically modified microorganism is used for treating inflammatory diseases or autoimmune diseases (such as enteritis and arthritis).

The present invention refers to the heterologous production in microorganisms of modified versions of a predicted isoform of the surfactant protein Lv-ranaspumin-1 (Lv-Rsn-1), whose sequence was inferred from analyzes of the protein extract of the nest foam from the Northeastern Pepper Frog (Leptodactylus vastus). More specifically, it refers to two surfactant proteins that consist of modified versions of the predicted isoform of Lv-Rsn-1; to two synthetic genes each encoding one of these modified versions of the predicted isoform of Lv-Rsn-1; to two expression cassettes each containing one of the synthetic genes encoding one of the modified versions of the predicted isoform of Lv-Rsn-1; to two expression vectors each containing one of the synthetic genes encoding modified versions of the predicted isoform of Lv-Rsn-1; and to two transgenic microorganisms, a bacterium and a yeast, each transformed with one of these synthetic genes and heterologously producing one of the modified versions of the predicted isoform of Lv-Rsn-1. Lv-Rsn-1 has surfactancy, emulsification and dispersancy properties, among others, and its heterologous production allows it to be used in various applications and industrial products, without the need to extract it from the frog nest foam.