115 results about "Genetically modified microorganisms" patented technology

The invention provides a method for producing borneol, the method comprising culturing the transgenic microorganism in a culture medium, and the microorganism comprises or expresses borneol diphosphate synthetase and phosphatase.

Disclosed is a genetically modified microorganism into which a nucleic acid encoding an enzyme that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is introduced. In the genetically modified microorganism, a nucleic acid encoding any one of the polypeptides described in (a) to (c) below is introduced or expression of the polypeptide is enhanced: (a) a polypeptide composed of an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 and 213, (b) a polypeptide composed of the same amino acid sequence as any one of the amino acid sequences, except that one or several amino acids are substituted, deleted, inserted, and / or added, and having enzymatic activity that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, and (c) a polypeptide composed of an amino acid sequence with a sequence identity of not less than 70% to any one of the amino acid sequences and having activity to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

A method for producing methionine, in particular, a method for producing methionine via condensation of 3-hydroxypropionaldehyde and C1-derived formyl-CoA was provided. Genetically modified microorganism used for producing methionine was also provided.

Provided herein are genetically modified microorganisms comprising a nonsense mutation in a gene encoding an efflux pump or import protein, and a vector encoding a suppressor tRNA that enables translation of the efflux pump or import protein, mitigating the effect of the nonsense mutation. Also provided are methods of producing a vector or markerless DNA using the genetically modified microorganisms provided herein.

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

The present disclosure relates to a genetically modified microorganism satisfying some of predetermined conditions. The predetermined conditions include: (I) succinate dehydrogenase activity or fumarate reductase activity being reduced or inactivated relative to a wild-type microorganism; (II) lactate dehydrogenase activity being reduced or inactivated relative to the wild-type microorganism; (III) the genetically modified microorganism having modified phosphoenolpyruvate carboxylase activity showing resistance to feedback inhibition by aspartic acid in wild-type phosphoenolpyruvate carboxylase activity, or exogenous phosphoenolpyruvate carboxylase activity having higher resistance to feedback inhibition by aspartic acid than that of the wild-type phosphoenolpyruvate carboxylase activity shown by the wild-type microorganism; and (IV) pyruvate:quinone oxidoreductase being reduced or inactivated relative to the wild-type microorganism.

Disclosed herein are genetically engineered Kluyveromyces sp. cells capable of producing D-allulose. The genetically engineered Kluyveromyces sp. cells comprise an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The invention includes novel routes of synthesis that involve the use of an acyloin condensation reaction between a ketone and formyl-CoA to form a corresponding branched 2-hydroxyacyl-CoAs and forming branched chain products therefrom. Described are genetically modified microorganisms that produce a branched product from a ketone and formyl-CoA and methods of producing a branched product, including in vivo and in vitro methods.

Disclosed herein are genetically engineered Kluyveromyces marxianus cells capable of producing D-allulose with increased talitol formation. The engineered cell may comprise a genetic modification resulting in overexpression of a native talitol dehydrogenase enzyme; an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

The present description relates to a transgenic microorganism capable of converting vegetable oils into olefins. The present description also discloses a process for obtaining such hydrocarbons from triglycerides or free fatty acids of plant origin, which is carried out by the microorganism disclosed in the present description.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Methods for producing organosulfur compounds from a prokaryotic cell comprising enzymes involved in the production of cysteamine, hypotaurine, and taurine. Said prokaryote may comprise a vanin selected from vanin-1 (vnn-1), vanin-2 (vnn-2), or vanin- 3 (vnn-3), a cysteamine dioxygenase (ado), and / or a flavin-containing monooxygenase 1 (fmo1). Methods include the expression of organosulfur compounds in Corynebacterium glutamicum with incubation conditions to promote compound production.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

The purpose of the present disclosure is to provide: a method for producing β-carotene and a β-carotene derivative; and a genetically modified microorganism. The present disclosure provides: a method for producing β-carotene and / or a β-carotene derivative using a genetically modified microorganism that belongs to the genus Lipomyces; and a genetically modified microorganism that belongs to the genus Lipomyces, and that has a β-carotene biosynthesis pathway and produces β-carotene and / or a β-carotene derivative through fermentation.

The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

The present invention relates to an enzyme cocktail, a method for the deconstruction of lignocellulosic biomass using said cocktail, and a genetically modified microorganism for producing heterologous enzyme aimed at making biomass fermentable into biorenewable products such as biofuels, organic acids, and hydrocarbon.

Disclosed is a genetically modified microorganism that can produce 3-hydroxyadipic acid and / or 3-oxoadipic acid in high productivity, and a method of producing 3-hydroxyadipic acid and / or 3-oxoadipic acid using the genetically modified microorganism. The genetically modified microorganism is a microorganism having an ability to produce 3-hydroxyadipic acid and / or 3-oxoadipic acid, in which the activity of an enzyme that catalyze the reaction to generate 3-oxoadipyl-CoA and CoA front succinyl-CoA and acetyl-CoA is enhanced, and the expression level of an organophosphate efflux transporter or a homolog thereof is increased.

A genetically modified microorganism that can produce 3-hydroxyadipic acid and / or α-hydromuconic acid with a high yield; and a method of producing 3-hydroxyadipic acid and / or α-hydromuconic acid using the genetically modified microorganism, are disclosed. The genetically modified microorganism has an ability to produce 3-hydroxyadipic acid and / or α-hydromuconic acid, and has an enhanced enzymatic activity to catalyze a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, wherein, in the genetically modified microorganism, a dicarboxylic acid excretion carrier function is deleted or decreased.

Among the various aspects of the present disclosure is the provision of a biological and biochemical production of piperazic acid derived from the newly discovered production pathway for L-piperazic acid. One aspect of the present disclosure includes a transgenic microorganism (e.g., bacteria) engineered to accumulate piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes biochemical and biological methods for producing piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes compositions and methods of using isotopically labeled piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product.

The invention pertains to the food industry and relates to new methods for producing casein compositions by culturing transgenic microorganisms in presence of lactoserum, and limiting the environmental impact.

The invention belongs to the food industry, relates to a novel method for producing casein compositions by culturing transgenic microorganisms in the presence of whey, and limits the environmental impact.

The present invention provides a method for producing liquid methionine or methionine, and particularly provides a method for producing liquid methionine or methionine by condensation reaction of 3-hydroxypropionaldehyde and formyl-CoA derived from a C1 substrate. The present invention also provides a genetically engineered microorganism for the production of liquid methionine or methionine.

The present invention relates to a method for the production of polyunsaturated fatty acids in an organism by introducing into the organism nucleic acids encoding polypeptides with Δ5-elongase activity. Advantageously, these nucleic acid sequences can be expressed in the organism if they are compatible with further nucleic acid sequences encoding polypeptides of fatty acid biosynthesis or lipid metabolism. Particularly advantageous are nucleic acid sequences encoding a Δ6-desaturase, Δ5-desaturase, Δ4-desaturase and / or Δ6-elongase activity. These desaturases and elongases are advantageously derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and / or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.In a preferred embodiment, the present invention further relates to a method for producing unsaturated ω3 fatty acids and a method for producing triglycerides with an elevated content of unsaturated fatty acids, especially ω3 fatty acids with more than three double bonds.The invention relates to the creation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an elevated content of unsaturated ω3 fatty acids, oils or lipids with ω3 double bonds as a result of expression of elongases and desaturases used in the method according to the invention, advantageously together with ω3 desaturases, for example an ω3 desaturase from fungi of the family Pythiaceae such as the genus Phytophthora, for example the genus and species Phytophthora infestans or an ω3 desaturase from algae such as the family Prasinophyceae, for example the genus Ostreococcus, specifically the genus and species Ostreococcus tauri or diatoms such as the genus Thalassiosira, specifically the genus and species Thalassiosira pseudonana.The invention further relates to nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences of the invention, vectors comprising the nucleic acid sequences and / or nucleic acid constructs and transgenic organisms comprising the above-mentioned nucleic acid sequences, nucleic acid constructs and / or vectors. A further part of the invention relates to oils, lipids and / or fatty acids produced by the method of the invention and their use. The invention further relates to unsaturated fatty acids and triglycerides with an elevated content of unsaturated fatty acids and their use.

Disclosed herein are genetically engineered Kluyveromyces marxianus cells capable of producing D-allulose. The genetically engineered Kluyveromyces marxianus cells comprise a deletion or disruption in a native PFK1 gene; an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:192, 194, and 196, and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

A process (1) for producing an article (2) from a plant-based material (3) includes providing (4) genetically modified microorganisms (5) in which the genetic modification (12) results in overexpression of at least one adhesive protein (6); mixing (7), preferably blending, of the plant-based material (3) with the genetically modified microorganisms (5), which are preferably held in a culture medium (11), to obtain a mixture (8); expressing of the adhesive protein (6); and hardening (10) of the mixture (8).

Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester are disclosed. For example, genetically modified methanotrophs that are capable of generating 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed. These microorganisms and methods make use of molecular switches to regulate gene expression.

A genetically modified microorganism that contains a gene capable of expressing a variant YeeX protein or a homolog thereof, said variant protein having an amino acid sequence derived from the amino acid sequence of a wild type YeeX protein or a homolog thereof by substitution, insertion and / or deletion of one to several amino acids. This genetically modified microorganism has an improved organic acid productivity compared to the microorganism before the genetic modification.

The present disclosure relates to genetically modified microorganisms for improving the phenotype of a plant, such as the nitrogen effectiveness of a leguminous plant. The present disclosure includes novel strains of microbial bodies, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important species, such as fixation, utilization, regulation, absorption, acquisition, tolerance and / or processing of nitrogen in plants.

Disclosed herein are genetically engineered yeast cells and genetically engineered bacterial cells capable of producing D-allulose. The genetically engineered cells comprise an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:192, 194, and 196, and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.