The invention discloses a NK (Natural Killer) cell with a chimeric CEA (Carcino-Embryonic Antigen) antigen receptor and a preparation method and applications thereof. The NK cell with the chimeric CEAantigen receptor, which is disclosed by the invention, is obtained by expressing the chimeric CEA antigen receptor in a NK cell. The NK cell with the chimeric CEA antigen receptor has the following characteristics that: 1, tumor cells can be efficiently and specifically killed; 2, while the CEA-CAR (Chimeric Antigen Receptor) is expressed, the regulatory mechanism of the NK cell per se is not affected, that is, the ability to inhibit the effect on homologous normal cells is not affected; and 3, after in vitro expansion of lymphocytes, the NK cell accounts for more than 90% of the harvested total cells and the CEA-CAR-NK accounts for more than 34% of the harvested total cells. The NK cell with the chimeric CEA antigen receptor, which is disclosed by the invention, plays an important role in clinical treatment of various solid tumors when serving as a biological agent.