The invention discloses a method for developing an SSR (Simple
Sequence Repeat) molecular mark of
nibea albiflora for
population identification. The method comprises the following steps: extracting
RNA (Ribonucleic Acid) by using a
nibea albiflora mixed tissue of a plurality of
nibea albiflora, performing
RNA sequencing, performing splicing to obtain a nibea albiflora
expression gene sequence, and obtaining a potential SSR mark with a
bioinformatics method; designing primers by utilizing an SSR flanking sequence, extracting
genomic DNA (
Deoxyribose Nucleic Acid) by using fins from a random sample, and screening the SSR primers; and performing PCR (
Polymerase Chain Reaction) amplification on different nibea albiflora
DNA templates by using each pair of the SSR primers, performing
capillary electrophoresis detection on an amplification product by using a Qsep100 full-automatic
nucleic acid analysis
system, screening out primers with different amplification results, calculating allelic composition and allelic frequency by utilizing PopGene, and analyzing a nibea albiflora
population relationship and a
genetic structure. The SSR molecular mark method for identifying and verifying nibea albiflora groups, provided by the invention, is stable and reliable, is not influenced by ages, gender and growth seasons, and provides scientific bases for group analysis as well as
species identification, breeding and evaluation of the nibea albiflora.