58 results about "Genetic architecture" patented technology

The invention belongs to molecular biology DNA labeling technique and application field, concretely relates to a porphyra yezoensis microsatellite marker screening method, and a method for analyzing germplasm genetic diversity by the makers, thereby lay foundations for genetic structure analysis of porphyra yezoensis, germplasm resource protection and molecular marker auxiliary breeding.

The invention relates to the field of genetic thremmatology of aquatic products, in particular to a multielement high flux genetic marking system and a genetic analyzing method of Chinese mitten crabs. The genetic marking system is a mitochondria sequence information system and a microsatellite molecule marking system, wherein the microsatellite molecule marking system comprises 7 EST-SSR systems marked 1and 6 EST-SSR marked systems 2. The method comprises the following steps: later generations produced by the same parent and different parents in a polyculture family are separated according to hereditary constitution and genetic diversity through information of sequences of three pairs of primer amplifications COI, Cytb and CR of mitochondria, and male parent discrimination is performed on later generations of the discriminated parents according to genetic distance and genetic diversity through the EST-SSR microsatellite molecule marking system, thereby completing the genealogy authentication during the genetic breeding of the Chinese mitten crabs and determining the inbreeding coefficients. The invention discriminates the genetic relationship among filial generations by using the multielement high flux genetic marking system on the basis of effectively utilizing species genetic information, and effectively avoids the inbreeding recession.

The invention discloses a molecular marker method for breeding meat performance of Altay sheep and application thereof. The molecular marker method comprises the following steps: using a MyoD1 (Myogenic Determination) gene as a candidate gene which influences meat yield of the Altay sheep; detecting a genetic structure of the MyoD1 gene in the Altay sheep variety by utilizing a PCR-SSCP (Polymerase Chain Reaction-Single-strand Conformation Polymorphism) technology; analyzing and judging a correlation between the genetic structure of the MyoD1 gene and the meat yield of the sheet; by a DNA (Deoxyribose Nucleic Acid) sequence of the MyoD1 gene of the sheet, self-designing two pairs of specific primers; carrying out polymorphism detection on the MyoD1 gene of the Altay sheep in Sinkiang province by utilizing the PCR-SSCP technology; and carrying out correlation analysis with meat performance so as to find a molecular marker which is obviously related to the meat yield of the Altay sheep. The molecular marker method is used for improving meat performance of the Altay sheep to greatly improve the speed of improving and recovering excellent meat performance of the Altay sheep and has important significance and practical application value for improving economic income of farmers and herdsmen and quickening development of the mutton sheep industry in Sinkiang province.

The invention discloses a method for developing an SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification. The method comprises the following steps: extracting RNA (Ribonucleic Acid) by using a nibea albiflora mixed tissue of a plurality of nibea albiflora, performing RNA sequencing, performing splicing to obtain a nibea albiflora expression gene sequence, and obtaining a potential SSR mark with a bioinformatics method; designing primers by utilizing an SSR flanking sequence, extracting genomic DNA (Deoxyribose Nucleic Acid) by using fins from a random sample, and screening the SSR primers; and performing PCR (Polymerase Chain Reaction) amplification on different nibea albiflora DNA templates by using each pair of the SSR primers, performing capillary electrophoresis detection on an amplification product by using a Qsep100 full-automatic nucleic acid analysis system, screening out primers with different amplification results, calculating allelic composition and allelic frequency by utilizing PopGene, and analyzing a nibea albiflora population relationship and a genetic structure. The SSR molecular mark method for identifying and verifying nibea albiflora groups, provided by the invention, is stable and reliable, is not influenced by ages, gender and growth seasons, and provides scientific bases for group analysis as well as species identification, breeding and evaluation of the nibea albiflora.

The invention discloses a method for classifying ctenopharyngodon idella based on an expressed sequence tag-simple sequence repeats (EST-SSR) marker, which comprises the following steps of: extracting the deoxyribonucleic acid (DNA) of the ctenopharyngodon idella, amplifying by using the EST-SSR marker, and analyzing the genetic structure of the ctenopharyngodon idella according to an amplification result. Compared with the conventional method, the method has the advantages of high purposiveness and direct action effect, and is easy to operate, high in detection speed, low in detection cost, and convenient to widely popularize and use.

The invention relates to the field of molecular biology of characters of fiber yield, in particular to a gene in significant correlation with the characters of the fiber yield, a SNP marker and application of theSNP marker. The gene in significant correlation with the characters of the fiber yield is a Gh_D05G1124 gene and / or a Gh_D05G0313 gene. 276 upland cotton materials are taken as raw materials and planted in multiple environments, a CottonSNP63k gene chip is adopted for genetic typing, and 10660 high-quality SNPs are obtained and used for analyzing of the genetic structureand GWAS. According to the result, 23 SNPs in significant correlation with the characters of the fiber yield are discovered to correspond to 15 QTLs. In addition, through Qrt-PCR analyzing, the Gh_D05G1124 gene andthe Gh_D05G0313 gene are taken as candidate genes for adjusting the characters of the fiber yield, the SNP marker in significant correlation with the fiber yield is obtained, and theoretical support is provided for research and application of the characters of the fiber yield.

The invention provides a tilapia mossambica and a microsatellite identification primer and method for genetic diversity of the tilapia mossambica; the microsatellite identification primer and method are included, and the method comprises the following steps: material selection, genome DNA extraction, microsatellite primer screening, microsatellite primer PCR reaction, electrophoresis detection anddyeing, and data analysis. According to the method, a later generation (F13, F14 and F15) group for breeding the new GIFT tilapia is used as a research object; tracking and monitoring the genetic variation of the later generation population by using a microsatellite marker technology; on one hand, genetic diversity levels of different breeding generations are compared, and the influence of the breeding process on the genetic structure of the breeding population is analyzed; on the other hand, microsatellite identification markers capable of distinguishing different breeding generation populations are searched, and through comprehensive analysis of the two aspects, a scientific basis is provided for continuous breeding and breed conservation work of the later generation of new GIFT tilapiamossambica breeding.

The invention discloses a polymorphic primer of a cinnamomum camphora nuclear genome SSR molecular marker and application thereof, and belongs to the technical field of forestry molecular biology. Thenucleotide sequence of the primer is shown as SEQ ID NO.1 to SEQ ID NO.14. The polymorphic primer is applied to the application of variety identification of cinnamomum camphora, and genetic structureand resource genetic diversity analysis of ancient cinnamomum camphora groups. A method includes the steps of genome DNA extraction, PCR amplification, polyacrylamide gel electrophoresis and data analysis. 186 individuals of 18 ancient cinnamomum camphora groups are subjected to genetic structure and resource genetic diversity study; the possibly forming reasons are analyzed according to existinggenetic structure and resource genetic diversity patterns of the cinnamomum camphora; and theoretical basis is provided for the wild cinnamomum camphora resource protection and utilization. The repeatability of the SSR molecular marker is high, and the real level of the genetic diversity of the cinnamomum camphora can be comprehensively disclosed.

The invention discloses polymorphic primers of a pheobe chekiangensis nuclear genome SSR molecular marker and an application of the polymorphic primers, and belongs to the technical field of forestry molecular biology. 15 pairs of polymorphic primers of the pheobe chekiangensis nuclear genome SSR molecular marker are developed through a PCR technology, a polyacrylamide gel electrophoresis technology and a capillary electrophoresis technology, and the nucleotide sequences of the primers are as shown in SEQ ID NO.1 to SEQ ID NO.30. The polymorphic primers of the pheobe chekiangensis nuclear genome SSR molecular marker are good in specificity and high in polymorphism; and when the polymorphic primers are applied to analysis of the genetic structure and resource genetic diversity of the pheobe chekiangensis, the diversity and the genetic structure of the genetic level of the pheobe chekiangensis in China can be disclosed more comprehensively, and a foundation is laid for formulating a genetic protection strategy.

The invention discloses a method for constructing tobacco core germplasm based on genomics and application of the method, and belongs to the technical field of molecular biology, genomics and germplasm resource science. The method comprises the steps: performing simplified genome sequencing on tobacco germplasm resource populations, adopting sequencing data quality control, SNP detection screeningand population stratification analysis, further dividing the whole germplasm resource large population into 200 subpopulations through IBS and K-means analysis, combining random selection in the subpopulations with background screening, and performing constructing to obtain the core germplasm capable of representing the whole tobacco germplasm resource large group. According to the method, the core germplasm construction efficiency can be improved, the genetic structure of an original population is well reserved, and the method has important significance in improving the germplasm resource utilization efficiency.

The invention provides a method for analyzing the corn borer population genetic diversity by an ISSR system, and relates to a method for analyzing the corn borer population genetic diversity. The invention aims at solving the problems that in the prior art, no effective molecular marking method, particularly an ISSR molecular marking method is used for identifying the corn borer, particularly thegenetic structure and the affinity relationship of different geographical populations of asiatic corn borer, so that the migration change condition of the corn borer is specified. The invention provides the method for analyzing the corn borer population genetic diversity by the ISSR system. The method is a simple and easy-to-operate molecular marking technology; for 14 ISSR primer sequences selected by the method, the PCR amplification reaction in the corn borer is stable; the amplified fragments are clear; the polymorphism is high; the technical support is provided for scientific research such as corn borer resource identification and genetic diversity analysis. The method is applied to the field of the corn borer.

The invention provides a method for detecting the genetic diversity and population genetic structure of wild Siniperca scherzeri. The method includes the following steps that firstly, DNA of different populations of wild Siniperca scherzeri is extracted respectively; secondly, primary PCR amplification and secondary PCR amplification are carried out on DNA, obtained in the first step, of wild Siniperca scherzeri respectively, and first amplification products and second amplification products of DNA of wild Siniperca scherzeri are obtained; thirdly, bidirectional sequencing is carried out on the first amplification products and the second amplification products of DNA of wild Siniperca scherzeri respectively, Cyt b gene sequences of the first amplification products and D-loop gene sequences of the second amplification products are spliced and combined, and sequences of wild Siniperca scherzeri Perciformes are obtained; fourthly, genetic diversity and population genetic structure analysis is carried out on the sequences of different populations of wild Siniperca scherzeri Perciformes. According to the method, combinatory analysis with double gene sequence splicing is adopted, the defect of insufficient information amount in single gene sequences is overcome, and the condition of the genetic diversity of species is more objectively and truly reflected.

The invention relates to the technology of the DNA molecular marker and provides a Scylla paramamosain microsatellite DNA marker and a screening method thereof. The Scylla paramamosain microsatellite DNA marker can be numbered to be A508. The screening method comprises the following steps: constructing a Scylla paramamosain microsatellite enriched library, selecting monoclone for sequencing; using a microsatellite DNA locus searching software to obtain the positive clone containing the Scylla paramamosain microsatellite sequence, and determining the Scylla paramamosain microsatellite DNA marker. The microsatellite marker A508 can be used for the genetic structure analysis of Scylla paramamosain, idioplasm resource protection and molecular marker-assisted selection, have good repeatability and be a reliable and effective molecular marker. The obtained Scylla paramamosain microsatellite DNA marker lays the foundation for the genetic structure analysis of Scylla paramamosain, idioplasm resource protection, molecular marker-assisted selection and the like.

The invention provides a method for researching genetic and propagation paths of a corn southern rust group based on an SNP (Single Nucleotide Polymorphism) marker. According to the method, DNA of different strains is subjected to enzyme digestion by using screened restriction enzymes EcoRI and MspI, so that a genome sequencing technology is simplified, SNP markers are mined by using Stacks software, and then the genetic structure and the propagation path of a puccinia multiflora population are researched; the restriction endonucleases EcoRI and MspI provided by the invention have a better cutting effect on the whole genome of puccinia multiflora, and compared with other combinations, more tags can be obtained under the condition of the same sequencing depth. At present, whole genome sequencing of puccinia multiflora is not performed yet, and the method for researching the population genetic structure and the propagation path of puccinia multiflora on the basis of the SNP sites obtained by simplifying genome sequencing and utilizing stacks software has important significance on comprehensive treatment and accurate prevention and control of diseases.

The invention provides EST-SSR (simple sequence repeat) fluorescent marker primers for scapharca broughtonii population genetic analysis and application and belongs to the field of molecular biologics. Sequences of the primers are shown in SEQ ID NO.1-10. The primers are adopted for genetic structure analysis on scapharca broughtonii populations, and effective tools can be provided for evaluatingand monitoring genetic structure variation of scapharca broughtonii populations in China.

The invention discloses a tea tree glutamine synthetase gene SSR molecular marker (SSR1-GS, SSR2-GS) primer and application of the tea tree glutamine synthetase gene SSR molecular marker primer. Researches show that the method for developing the SSR primer on the whole genome of the tea tree according to specific genes is feasible, and the obtained primer is high in polymorphism and good in repeatability and has good feasibility and pertinence. Therefore, the inventor develops research on genetic polymorphism of tea tree germplasm resources. In conclusion, the research method, the SSR molecular marker and the primer of the SSR molecular marker can be widely applied to tea tree variety identification, genetic structure and resource diversity analysis, genetic map construction, functional gene positioning and QTL positioning, seed purity identification and molecular marker-assisted seed selection research, and tea tree theanine synthesis and tea tree genetic polymorphism can be further researched conveniently; deep development of tea tree resources is promoted.

The invention discloses a grass carp classification method based on EST-SSR markers. The method includes extracting the DNA of the grass carp, using EST-SSR markers to amplify, and analyzing the genetic structure of the grass carp according to the amplification result. Compared with the traditional method, the method of the invention has the advantages of strong purpose and direct effect. And the operation is simple, the detection is fast, the detection cost is low, and it is convenient to be popularized and used widely.

The invention discloses a polymorphic primer of a cinnamomum camphora nuclear genome SSR molecular marker and application thereof, and belongs to the technical field of forestry molecular biology. Thenucleotide sequence of the primer is shown as SEQ ID NO.1 to SEQ ID NO.14. The polymorphic primer is applied to the application of variety identification of cinnamomum camphora, and genetic structureand resource genetic diversity analysis of ancient cinnamomum camphora groups. A method includes the steps of genome DNA extraction, PCR amplification, polyacrylamide gel electrophoresis and data analysis. 186 individuals of 18 ancient cinnamomum camphora groups are subjected to genetic structure and resource genetic diversity study; the possibly forming reasons are analyzed according to existinggenetic structure and resource genetic diversity patterns of the cinnamomum camphora; and theoretical basis is provided for the wild cinnamomum camphora resource protection and utilization. The repeatability of the SSR molecular marker is high, and the real level of the genetic diversity of the cinnamomum camphora can be comprehensively disclosed.

The invention relates to a narcissus BES-SSR marker primer GA19 and a method for identifying narcissus species. The method comprises the following steps: design and synthesis of the narcissus BES-SSR marker primer GA19, extraction of total DNA (deoxyribonucleic acid) from a narcissus genome, establishment of a PCR (polymerase chain reaction) total reaction system, establishment of PCR amplification programs, establishment of polyacrylamide gel electrophoresis conditions, and detection and identification of PCR amplification products. A narcissus BES-SSR marker developed by a BAC-terminal sequence of the narcissus genome is adopted, has the characteristics of high accuracy, easiness in judgment, good repeatability, effect of not being affected by environmental factors, clear spectral band and the like, is an effective and reliable molecular marker, and further has the advantages of small sampling quantity, convenience in operation, reliable results and the like when used for identifying the narcissus species. The narcissus BES-SSR marker primer GA19 can also be used for drawing of a narcissus species fingerprint spectrum, analysis of genetic diversity, research of a population genetic structure, analysis of a genetic linkage map and other technical fields.

The invention discloses an SSR marker based primer set for identifying germplasm resource genetic relationship of halogeton glomeratus. A total of 33 pairs of SSR polymorphic molecular markers are identified, an accurate and efficient method for identifying the genetic structure of the halophyte germplasm from different sources is established, and reference can be provided for identifying the halophyte germplasm from different sources. The method for identifying the halophyte germplasm resources comprises the steps of halogeton glomeratus DNA extraction, SSR primer amplification, polymorphismscreening, classification of halogeton glomeratus materials according to amplification results and the like. The germplasm resource genetic relationship of the halogeton glomeratus can be identified accurately and efficiently.

The invention discloses an application of 32 soybean InDel markers in detection of soybean genetic diversity. The 32 soybean InDel markers are 32 DNA molecules obtained by performing PCR amplification on soybeans by using 32 pairs of primers as shown in sequences 1-64 in a sequence table. The 32 soybean InDel markers can be used for detecting the soybean genetic diversity, identifying soybean varieties or strains or developing molecular markers for identifying the soybean varieties or strains, preparing soybean genetic maps, analyzing soybean genetic structures and further breeding soybeans. The 32 soybean InDel markers and the complete set of primers for detecting the 32 soybean InDel markers have wide research prospects.

The invention discloses primers and a method for constructing cynodon dactylon core germplasm by using a SCoT molecular marker. The sequences of the primers are shown as SEQ ID NO: 1-19, and the primers are applied to construction of the cynodon dactylon core germplasm. The 19 primer strips disclosed by the invention are clear, high in polymorphism and good in repeatability, and a cynodon dactylonSCoT amplification result shows that the 19 primer strips have rich genetic diversity and are suitable for constructing core germplasm. The cynodon dactylon SCoTCore disclosed by the invention generally maintains the genetic structure of an original population, the redundant materials are effectively removed, and the cynodon dactylon SCoTCore is a qualified core germplasm and is representative ofthe original germplasm.

The invention provides a group of plateau zokor polymorphic microsatellite molecular markers, and belongs to the technical field of DNA molecular markers. The microsatellite molecular marker(s) is / are a nucleotide sequence(s) as shown in any one or more of SEQ ID NO: 1 to SEQ ID NO: 10. Through high-throughput sequencing technology and by using a bioinformatics method, the invention screens microsatellite-containing DNA sequences, designs specific primers, and performs polymorphism detection on these microsatellite loci, and develop a group of (10) plateau zokor microsatellite molecular markers with abundant polymorphisms, and provides a set of plateau zokor microsatellite primers. The plateau zokor microsatellite primer pair provided by the invention can perform specific amplification on microsatellite loci, has high polymorphism, can be applied to the research fields of genetic diversity, population genetic structure, pedigree geography, evolution and genetic relationship and the like of the plateau zokor and related species thereof, and has excellent application prospects.