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Method for detecting genetic diversity and population genetic structure of wild Siniperca scherzeri

A technology of genetic diversity and detection method, applied in the field of detection of wild spotted mandarin fish

Pending Publication Date: 2016-08-17
SOUTH CHINA AGRI UNIV
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  • Abstract
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Problems solved by technology

At present, there are few literature reports on the population genetics and diversity of spotted mandarin fish

Method used

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  • Method for detecting genetic diversity and population genetic structure of wild Siniperca scherzeri
  • Method for detecting genetic diversity and population genetic structure of wild Siniperca scherzeri
  • Method for detecting genetic diversity and population genetic structure of wild Siniperca scherzeri

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with specific embodiments. However, it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention.

[0028] 1 Materials and methods

[0029] 1.1 Test material

[0030] From June 2013 to August 2013, they were collected in the Hongshuihe section of the Guangxi Basin (coded HSH group), the Duyun section of the Guizhou basin (coded DY) and the Dongjiang Fengshuba Reservoir (coded BL) in the Pearl River system. There are 114 samples of mandarin fish in total, all of which are wild populations. The back muscles of the fish were taken from the field, dehydrated with 95% ethanol, placed in absolute ethanol, brought back to the laboratory, and stored at low temperature.

[0031] 1.2 Test method

[0032] Genomic ...

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Abstract

The invention provides a method for detecting the genetic diversity and population genetic structure of wild Siniperca scherzeri. The method includes the following steps that firstly, DNA of different populations of wild Siniperca scherzeri is extracted respectively; secondly, primary PCR amplification and secondary PCR amplification are carried out on DNA, obtained in the first step, of wild Siniperca scherzeri respectively, and first amplification products and second amplification products of DNA of wild Siniperca scherzeri are obtained; thirdly, bidirectional sequencing is carried out on the first amplification products and the second amplification products of DNA of wild Siniperca scherzeri respectively, Cyt b gene sequences of the first amplification products and D-loop gene sequences of the second amplification products are spliced and combined, and sequences of wild Siniperca scherzeri Perciformes are obtained; fourthly, genetic diversity and population genetic structure analysis is carried out on the sequences of different populations of wild Siniperca scherzeri Perciformes. According to the method, combinatory analysis with double gene sequence splicing is adopted, the defect of insufficient information amount in single gene sequences is overcome, and the condition of the genetic diversity of species is more objectively and truly reflected.

Description

technical field [0001] The invention relates to the technical field of detection of wild mandarin fish, in particular to a method for detecting genetic diversity and population genetic structure of wild mandarin mandarin fish. Background technique [0002] Siniperca scherzeri (Siniperca scherzeri) belongs to the genus Siniperca of the family Serranidae in the order Perciformes. It is a rare and rare freshwater fish endemic to East Asia and is widely distributed in various inland watersheds of China. Its meat is tender, delicious, and has no intermuscular thorns. It is known as "freshwater grouper" and is popular with consumers in China, South Korea, Singapore and other places. It is one of my country's important freshwater export fishes. In recent years, due to the destruction of the water environment and the unhealthy use of fishery resources, the population of wild spotted mandarin fish has tended to decline: our research group did not find a large number of wild spotted m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/172C12Q2531/113
Inventor 刘丽
Owner SOUTH CHINA AGRI UNIV
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