Tilapia mossambica and microsatellite identification primer and method for genetic diversity of tilapia mossambica

Abstract

The invention provides a tilapia mossambica and a microsatellite identification primer and method for genetic diversity of the tilapia mossambica; the microsatellite identification primer and method are included, and the method comprises the following steps: material selection, genome DNA extraction, microsatellite primer screening, microsatellite primer PCR reaction, electrophoresis detection anddyeing, and data analysis. According to the method, a later generation (F13, F14 and F15) group for breeding the new GIFT tilapia is used as a research object; tracking and monitoring the genetic variation of the later generation population by using a microsatellite marker technology; on one hand, genetic diversity levels of different breeding generations are compared, and the influence of the breeding process on the genetic structure of the breeding population is analyzed; on the other hand, microsatellite identification markers capable of distinguishing different breeding generation populations are searched, and through comprehensive analysis of the two aspects, a scientific basis is provided for continuous breeding and breed conservation work of the later generation of new GIFT tilapiamossambica breeding.

Description

technical field [0001] The invention relates to the technical field of fish breeding, in particular to a microsatellite identification primer and method for tilapia and its genetic diversity. Background technique [0002] "Xin Jifu" tilapia is the third generation of Nile tilapia "GIFT" strain introduced by Shanghai Fisheries University in 1994 as the basic group (F0) (Li Sifa, 2001), and has been systematically selected for 10 years since 1996 The new variety formed, its F8 was approved and named by the National Aquatic Original Species and Improved Variety Approval Committee in January 2006 (registration number GS01-001-2005), and the Ministry of Agriculture announced (No. 631) that it was an excellent variety promoted nationwide (Li Sifa et al., 2008). In view of the concept that there is no permanent good breed and the requirement of the era of innovation, on the basis of the breeding line F8, the research team insisted on the continuous breeding of "Xinjifu" tilapia. B...

AI Technical Summary

Problems solved by technology

However, with the artificial breeding process, the closed breeding population is vulnerable to non-random mating, genetic drift, artificial directional selection pressure and other factors, resulting in a decrease in the number of effective populations and a

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