240 results about "Tilapia sp." patented technology

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention relates to the technical field of gene engineering, and particularly discloses streptococcus agalactiae WC1535 delta cps delta hylB as well as a construction method and application thereof. The streptococcus agalactiae WC1535 [delta] cps [delta] hylB is preserved in Guangdong Microbial Culture Collection Center on February 28, 2025, the preservation number is GDMCC NO.65955, and the address of the preservation unit is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Compared with a traditional single-fragment deletion method, the toxicity of the deletion strain is not prone to being enhanced, and compared with a traditional injection immune vaccine, the gene deletion strain is used for preparing the tilapia oral live vaccine, the immune operation is simpler, and more labor cost is saved.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention belongs to the field of computer vision, and provides a tilapia behavior image detection method, system and equipment, and the method comprises the steps: enabling the fish mouth feature vectors of a plurality of samples to form a feature matrix, generating a fish mouth feature alignment index matrix, and extracting a target alignment vector and a reference alignment vector; and respectively calculating the inner product of the alignment index vector of each sample, the target alignment vector and the reference alignment vector to obtain a target alignment score and a reference alignment score, taking the distance between the target alignment score and the reference alignment score as a behavior offset index, and mapping the behavior offset index as the probability of the parent hatch behavior. The method has natural robustness, can restrain the influence of accidental noise, can effectively recognize the female incubation behavior in the underwater complex environment, and has higher real-time response capability in actual aquaculture monitoring.

The invention relates to the technical field of biology, in particular to a detection method for tilapia streptococcus agalactiae resistance, a KASP primer group and application. According to the invention, the key SNP site of the ABCB4 gene, which is obviously associated with the streptococcus agalactiae disease resistance, of the tilapia is identified by a population genetics method, a typing method of the SNP site is developed by a KASP technology, efficient evaluation on disease resistance characters is realized, experimental data shows that the survival time of an AA genotype individual in a challenge test is prolonged compared with that of a GA / GG genotype individual, and the survival time of the AA genotype individual in the challenge test is obviously prolonged compared with that of a GA / GG genotype individual. The proportion of resistant fishes is remarkably increased, and genotype frequency distribution is strongly related to group disease resistance. According to the breeding strategy based on the SNP marker, the disease resistance of bred groups can be integrally improved, the morbidity and death rate are reduced, an efficient genetic improvement tool is provided for breeding of tilapia mossambica for resisting streptococcus agalactiae diseases, the loss of breeding diseases is reduced, and antibiotic dependence is reduced.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) marker combination for identifying tilapia streptococcus agalactiae disease resistance and application thereof. The invention discloses 11 SNP loci significantly related to streptococcus agalactiae resistance in a tilapia UTRN gene, and constructs 18 SNP marker combinations for streptococcus agalactiae resistance phenotype identification, so that the resistance phenotype identification accuracy and hereditary stability can be effectively improved. Besides, according to the SNP typing primer and method based on the # imgabs0 # MALDI-TOF mass spectrum platform, high-throughput and high-precision genetic typing can be achieved, and the SNP typing primer and method have important application value in resistance phenotype identification and assistant selective breeding.

The invention relates to the technical field of biology, in particular to a tilapia mossambica streptococcus agalactiae resistance related KASP molecular marker and application thereof. According to the invention, two SNP (Single Nucleotide Polymorphism) loci, namely SNP-1 and SNP-2, which are remarkably associated with resistance are screened based on an intron region of an EGFRX2 gene of tilapia mossambica, a typing method of the loci is developed through a KASP technology, and relevance between the genotype of the SNP-1 and a resistance phenotype is verified. Furthermore, streptococcus agalactiae resistance dominant diplotype individuals are screened in combination with double SNP loci, and the survival rate of a challenge experiment is remarkably improved. The detection kit provided by the invention can realize high-throughput and high-precision typing, is suitable for tilapia mossambica resistance genotype identification and molecular marker-assisted breeding, provides an efficient genetic improvement tool for tilapia mossambica disease-resistant breeding, and is beneficial to reduction of breeding disease loss and reduction of antibiotic dependence.

The invention discloses a tilapia mossambica processing device which comprises a workbench, a packaging box is attached to the top of the workbench, a baffle is fixedly connected to the position, close to the left side, of the top of the workbench, two conical blocks are fixedly connected to the bottom of the workbench, the two conical blocks are arranged in a bilateral symmetry mode, and two connecting plates are arranged at the bottom of the workbench; through mutual cooperation of the component workbench, the L-shaped fixing plate, the baffles, the connecting plates, a transverse rod, a transverse plate, a swing plate, an attaching plate, a stabilizing plate, a movable plate, a bottom plate, a balance plate, a balancing weight, a linkage rod, a hinge block and other components, a user can firstly adjust the position of the balancing weight; and when the tilapia mossambica is added into the packaging box, the balance plate can be horizontally stable, so that the weight of the tilapia mossambica in the packaging box is consistent.

The invention relates to the technical field of collagen peptide preparation, and discloses a collagen peptide as well as a preparation method and application thereof. The preparation method of the collagen peptide comprises the following steps: adding water into Hefang crucian carp skin, regulating the Hefang crucian carp skin into feed liquid with the optimal pH value of protease, then adding the protease into the feed liquid for enzymolysis, inactivating after enzymolysis, and centrifuging to take supernate, so as to obtain the collagen peptide. The protease comprises one or more than two of alkaline protease, animal protease and neutral protease. According to the present invention, the He-prescription crucian carp skin is adopted as the preparation raw material, the protease most suitable for the raw material is adopted to perform enzymolysis to prepare the collagen peptide, the optimal enzymolysis process parameters are determined, and compared with the conventional tilapia skin collagen peptide, Hunan crucian carp skin collagen peptide and collagen tripeptide in the antioxidant function, the antioxidant activity is significantly improved, and the antioxidant activity is significantly improved; therefore, the method for preparing the crucian carp skin collagen peptide with remarkable antioxidant activity is established, and the application range of crucian carp processing byproducts is further expanded.

The invention belongs to the field of tilapia mossambica breeding, and discloses a rapid and efficient tilapia mossambica breeding method and system.The method comprises the steps that tilapia mossambica populations with excellent character potential are collected and screened, and a basic breeding population is established; molecular markers related to target characters are developed, and individuals carrying excellent genes are rapidly screened; a target gene is precisely regulated and controlled through a CRISPR / Cas9 technology, so that stable inheritance of excellent characters is accelerated; phenotypes such as growth performance, disease resistance and environment resistance are efficiently evaluated and screened; the excellent characters are fixed; and the performance of the new strain under the actual breeding condition is evaluated. The efficient and low-cost tilapia mossambica breeding method is constructed by integrating molecular marker, gene editing and phenotype screening technologies, the breeding efficiency and the commercialization value of a new strain are remarkably improved, and powerful technical support is provided for the aquaculture industry.

The invention relates to the technical field of genomics and metabonomics, in particular to a method for analyzing intestinal health of crisp tilapia mossambica. The tilapia mossambica intestinal health judgment model is established by dynamically monitoring tilapia mossambica muscle texture and intestinal morphology through high time resolution, analyzing intestinal health three-dimensional indexes (tissue morphology-flora structure-host gene expression) and serum metabolism response after embrittlement succeeds, and combining cross-omics data association analysis. The change condition of the muscle texture can be known more timely, a more accurate basis is provided for adjustment of a feeding strategy, and the stability of the fish meat quality can be improved. The intestinal health is evaluated from three dimensions of histomorphology, flora structure and host gene expression, so that the health condition of the intestinal tract can be known more comprehensively, and more comprehensive reference is provided for optimization of a feeding method. The correlation between different omics data can be deeply analyzed, the physiological and metabolic mechanisms of the fish body are disclosed, and deeper theoretical support is provided for optimization of a feeding method.

The application discloses a hybridoma cell strain secreting an anti-tilapia interleukin-2 monoclonal antibody and an application thereof, and the hybridoma cell strain 1C11F5 is preserved in the China Center for Type Culture Collection (CCTCC) on March 9, 2024, and the preservation number is CCTCC NO: C202470. The application can specifically identify the tilapia IL-2 protein and the lymphocyte group secreting IL-2, is helpful for further exploration of T cell activation and proliferation, and provides an important immunological tool for adaptive immune mechanism research of hard fish (such as tilapia and the like).

The invention provides a crispness grading method for crisp tilapia, and relates to the technical field of tilapia quality detection. The method for grading the crispness of the crisp tilapia mossambica comprises the following steps: determining the crispness of the raw crisp tilapia mossambica by adopting a texture analyzer, setting a sensory evaluation crispness grade for the cooked crisp tilapia mossambica, simultaneously performing sensory evaluation, and performing correlation analysis on the crispness value of the raw crisp tilapia mossambica and the sensory score of the cooked crisp tilapia mossambica. Therefore, the crispness value of the raw crisp-meat tilapia and the grading contrast condition of the cooked crisp-meat tilapia are set. According to the method, the defects in the prior art are overcome, and the sensory evaluation crispness grade of the cooked crisp tilapia can be predicted according to the meat crispness detection result of the raw crisp tilapia, so that reliable reference is provided for transaction pricing, breeding process crispness control, commodity grading and processing process quality control of the crisp tilapia.

The utility model discloses a thawing device of tilapia processing, including support frame still including the deflector that makes fish body slide and the deflector of surface of installation in support frame, the baffle of fixed connection on deflector, the intercepting assembly of installation on baffle and intercept fish body, thawing assembly of installation on baffle, the liquid collecting assembly of installation on support frame and the guide assembly of installation on liquid collecting assembly, fish body moves on deflector, and thawing assembly is used for spraying water to fish body, and the wastewater of thawing is collected through liquid collecting assembly, and the fish body of thawing is finished and passes through the guide assembly empty part moisture while from the liquid collecting assembly top. The deflector of ladder form makes fish body slide under the action of gravity and turns over naturally, ensures that each surface of fish body can evenly receive thawing water, effectively eliminates "thawing dead angle", avoids the forced turning of rigid parts such as mechanical arm, prong in whole process, maximum degree reduces muscle tissue damage, and can guarantee the continuity of thawing process.

1. Name of the product in this design: Fish bait (imitating tilapia). 2. Purpose of this design: Fishing tackle for fishing. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The utility model relates to the field of tilapia mossambica cleaning devices, and discloses a tilapia mossambica processing and cleaning device which comprises a fixing support, a cleaning shell is fixedly mounted in the fixing support, two partition plates are fixedly mounted in the cleaning shell, and a plurality of water permeable holes are formed in the top surfaces of the two partition plates; an exhaust shell is fixedly installed at the position, below the partition plate, in the cleaning shell, a plurality of exhaust nozzles corresponding to the water permeable holes are fixedly installed on the top face of the exhaust shell, and compressed air generated by an external air pump is guided into the exhaust shell through an air guide pipe and sprayed out through the exhaust nozzles; the jetted air forms upward airflow in the cleaning shell, so that the fillets soaked in the clear water roll over, impurities and blood attached to the surfaces of the fillets can be effectively removed through rolling over, the cleaning effect is remarkably improved, and sanitation and quality of the fillets in the cleaning process are guaranteed.

The present application relates to the field of biotechnology, and particularly relates to a KASP molecular marker related to the resistance of tilapia to streptococcus agalactiae disease and an application thereof.Two SNP sites (SNP-1 and SNP-2) significantly associated with the resistance are screened from the intron region of EGFRX2 gene of tilapia, a typing method of the above sites is developed through KASP technology, and the correlation between the SNP-1 genotype and the resistance phenotype is verified.Further, an advantage haplotype individual with the resistance to streptococcus agalactiae disease is screened by combining the two SNP sites, and the survival rate of the individual in the challenge experiment is significantly improved.The detection kit provided by the present application can realize high-throughput and high-precision typing, is suitable for the identification of the resistance genotype of tilapia and the marker-assisted selection, provides an efficient genetic improvement tool for the disease-resistant breeding of tilapia, and is helpful to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The present application belongs to the technical field of food processing, and relates to a method for removing earthy smell of tilapia fillets. The method comprises the following steps: covering the surface of the tilapia fillets with pleurotus eryngii powder, and placing the tilapia fillets in a deodorizing and fresh-keeping liquid for 30-60 min; the deodorizing and fresh-keeping liquid is composed of ginger powder 15-20 parts, cannabis seed protein 5-10 parts, lysozyme 0.5-1 part, tea polyphenol 4-8 parts and water 60-100 parts in terms of mass fraction. The present application solves the technical problem of long-term maintenance of fresh fish flavor when removing the earthy smell of tilapia fillets.

The invention relates to the technical field of food processing, in particular to an alkali-induced glycosylated tilapia protein hydrolysate loaded with fat-soluble polyphenol as well as a preparation method and application of the alkali-induced glycosylated tilapia protein hydrolysate. The glycosylated tilapia mossambica protein hydrolysate is prepared by adopting flavourzyme with mild action conditions for moderate hydrolysis and combining with a green, efficient and reaction degree-adjustable alkali-induced propylene glycol alginate (PGA) glycosylation strategy, so that the water solubility and emulsibility of tilapia mossambica protein are effectively improved, and the tilapia mossambica protein hydrolysate can be used for preparing the tilapia mossambica protein hydrolysate. The glycosylated protein hydrolysate (modified tilapia protein) is used as a carrier to develop an oral delivery system of fat-soluble polyphenols such as quercetin and the like, so that the bioaccessibility of the fat-soluble polyphenols is improved, and the fat-soluble polyphenols can be developed into functional food ingredients to improve the health condition of special groups; the high-value utilization of the tilapia protein in the protein-based polyphenol functional food ingredients is promoted.

The invention provides a functional feed additive for improving the quality of tilapia mossambica. The functional feed additive comprises the following raw materials: hydrolyzed fish meal, fermented soybean meal, insect protein powder, fish soluble, callicarpa nudiflora powder, fish oil, polysaccharide, an intestine regulating agent and vitamins. The preparation method of the hydrolyzed fish meal comprises the following steps: crushing fish, adding a Na2CO3 solution for rinsing, performing high-temperature and high-pressure squeezing for degreasing, adding a mixed enzyme solution for enzymolysis, performing enzyme deactivation for 2-3 times, performing centrifugal separation, concentrating, drying, crushing and sieving. Wherein the polysaccharide is fucoidin and trametes robiniophila polysaccharide, and the intestine regulating agent is lactobacillus rhamnosus powder and lactobacillus kefir powder. The feed additive is balanced in formula nutrition, improves the quality of tilapia mossambica, reduces intestinal diseases of tilapia mossambica, and has application value.

The present invention relates to the effect of promoting muscle development and growth through inhibition of the expression of MRF4. According to the present invention, MRF4 isolated and identified from skeletal muscles of Nile tilapia showed a strong association with MyoG and MEF2 proteins, and the expression of MyoG and MEF2 was found to significantly increase when the MRF4 gene was disrupted through CRISPR / Cas9 gene editing, and thus the present invention has the effect of being able to be used for the promotion of muscle development or the treatment of muscle diseases through the inhibition of the MRF4 gene.

The invention discloses a tilapia scaling quality classification method and system based on hyperspectrum and machine learning, and the method comprises the steps: obtaining the hyperspectral information of tilapia with different scaling qualities, carrying out the spectrum preprocessing, and obtaining the hyperspectral data without noise and background; obtaining a hyperspectral data set of the tilapia mossambica with different scaling qualities; constructing a tilapia scaling quality identification model based on a convolutional neural network and an SVM algorithm, inputting the hyperspectral data sets of various tilapia with different scaling qualities into the model for training, and obtaining an optimal model through training; hyperspectral information of to-be-detected scaled tilapia mossambica is obtained and input into the optimal model for detection, and according to an identification result, the classification device classifies the tilapia mossambica of which the scaling quality does not reach the standard. According to the method, the classification of the tilapia mossambica with unqualified scaling quality can be effectively realized, and the efficiency and the accuracy of the scaling quality detection of the tilapia mossambica are improved to a great extent.

、To provide a method for determining the smell of fish, especially the degree of smell deterioration and lipid oxidation of tilapia during the refrigeration period. 【Solution means】Steps of obtaining raw materials by killing fresh tilapia, taking slices, washing, and mixing and grinding the fish meat; steps of extracting lipids from the raw materials; steps of dividing the obtained lipids, storing them under low-temperature conditions, and measuring the degree of lipid oxidation and lipid metabolism data of tilapia at different refrigeration times by lipid metabolomics technology after the storage ends; steps of constructing a lipid metabolism network related to smell and analyzing the degree of lipid oxidation and metabolism data at different refrigeration times based on the lipid metabolism network to obtain metabolic markers corresponding to a plurality of lipids; steps of screening the obtained metabolic markers to obtain evaluation metabolic markers; steps of analyzing the degree of lipid oxidation and metabolic status of a plurality of lipids based on the evaluation metabolic markers corresponding to the plurality of lipids.

The application discloses a recombinant strain and a preparation method thereof and application of the recombinant strain in preparation of a vaccine for Streptococcus agalactiae of tilapia, and the recombinant strain is successfully constructed by the following steps: designing an ORF4 primer, amplifying an ORF4 gene fragment from a DNA template of Streptococcus agalactiae, editing the ORF4 gene fragment into Escherichia coli by means of a CRISPR / Cas9 gene editing technology, and connecting a green fluorescent protein (GFP). The recombinant strain is subjected to immunoprotection research on the Streptococcus agalactiae of tilapia, and it is shown that the recombinant strain has a good protection effect, and can provide a solid theoretical basis and technical support for application and development of the vaccine for Streptococcus agalactiae of tilapia in the field of aquaculture.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

To provide a method for determining odor of fish, especially odor deterioration and lipid oxidation degree of tilapia during cold storage.SOLUTION: The method includes the steps of: killing fresh tilapia, slicing, washing, mixing and grinding the fish meat to obtain raw materials; extracting lipids from the raw materials; dividing the obtained lipids and storing them under low-temperature conditions, and after storage, measuring the lipid oxidation degree and lipid metabolic data of tilapia at different refrigeration times using lipid metabolomics technology; constructing an odor-related lipid metabolic network and analyzing the lipid oxidation degree and metabolic data at different refrigeration times based on the lipid metabolic network to obtain metabolic markers corresponding to multiple lipids; screening the obtained metabolic markers to obtain evaluation metabolic markers; and analyzing the oxidation degree and metabolic status of multiple lipids based on the evaluation metabolic markers corresponding to multiple lipids.SELECTED DRAWING: Figure 1

The application discloses a strain of Sideropenaeus paramamosain bio-floc high-pond Shajia external sulfur red spirillum HDES1 and application of the strain in purifying inorganic nitrogen and phosphorus in aquaculture water, and belongs to the technical field of microorganisms. The Shajia external sulfur red spirillum HDES1 in the application is screened from a Sideropenaeus paramamosain bio-floc aquaculture high-pond, and has a preservation number of CCTCC NO: M 20191007. It is found through experiments that the strain has a remarkable purifying effect on high-concentration inorganic nitrogen and phosphorus in seawater aquaculture water or tail water, has no adverse effect on cultured Sideropenaeus paramamosain and seawater tilapia, and is helpful to improving the yield of fish and shrimp culture and realizing zero-water-change green ecological culture.

The invention relates to a recombinant kunitz protein of third-generation botryosphaeria formosa as well as a preparation method and application of the recombinant kunitz protein, and belongs to the technical field of fish parasite protein expression and function application research. The serine protease inhibitor of the third-generation botryosphaeria formosa is a GcKSI protein, and the amino acid sequence of the GcKSI protein is as shown in SEQ ID NO: 2. According to the invention, the amino acid sequence of the GcKSI protein is optimized, and the GcKSI recombinant protein rGcKSI is obtained through prokaryotic expression. The rGcKSI inhibits complement activity of host fish in vitro and has an obvious inhibition effect on serine protease, after injection immunization of the rGcKSI and third-generation insect infection experiments, the infection rate and the average infection intensity of the rGcKSI are obviously lower than those of a control group, and it is proved that the rGcKSI has good immunoprotectiveness on third-generation insect infection, and the rGcKSI can be used as an immunopotentiator for the third-generation insect infection of the tilapia. The GcKSI gene of the serine protease inhibitor and the GcKSI protein coded by the GcKSI gene can be used as new targets for drug research and development.

The invention discloses a composition for improving the immunity of aquatic organisms, and particularly belongs to the field of functional feeds, and the composition for improving the immunity of aquatic organisms comprises the following components in parts by mass: 20-30 parts of sedum sarmentosum extract, 30-40 parts of chenopodium ambrosioides extract and 10-15 parts of perilla frutescens extract. The composition further comprises 4-6 parts by mass of astaxanthin and 7-9 parts by mass of vitamin C phosphate. In the composition provided by the invention, the sedum sarmentosum extract, the chenopodium ambrosioides extract and the perilla frutescens extract have a certain synergistic effect in the aspect of improving the immunity of tilapia mossambica, and after astaxanthin and vitamin C phosphate are added into the composition, the effect is better. The feed containing the composition for improving the immunity of aquatic organisms can significantly improve the non-specific immunity of tilapia mossambica.