461 results about "Tilapia sp." patented technology

A method for preparing ACE inhibition peptides from fish skin is provided. The method is prepared with tilapia skin, extracts fish skin albumen from tilapia skin, adapts prolease to hydrolysis through enzyme extinguishing, debitterizing, decolouring, de-flavoring, filtering, ion exchanging and concentrating to obtain fish skin proteolysis liquor, after untruly filter (cutoff molecular mass is 9000Da) the TSP-I, collect high activity component by SephadexG-15 gel chromatography, freeze and dry to obtain high activity ACE inhibition peptides. The invention has important meaning for satisfying integrated utilization of tilapia skin wastes and improving its attachment.

The invention provides a collagen and a collagen polypeptide, wherein, the weight percentage of hydroxylysine is more than 1.3 percent. The invention also provides the preparation technology and the application of the collagen and the collagen polypeptide. The invention can make full use of inexpensive raw materials including the fish skin and fish scale of tilapias to produce products with high added value. The collagen polypeptide is used for treating arthritis with a definite drug effect, and the clinical dose is considerably less than other collagen polypeptides. The collagen polypeptide can solve the pollution problem of fish skin wastes, and at the same time avoid the risk of infectious diseases which can be caused by the collagen of land animals. The collagen and the collagen polypeptide have the advantages of considerable economic benefits, energy conservation and environmental protection, simple process equipment, low cost and easy industrialization.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU/g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention discloses a compound feed for preventing fatty liver of tilapia and a preparation method of the feed. The compound feed is prepared from the following raw materials according to a certain proportion: fish meal, fermented soybean meal, meat and bone meal, rapeseed meal, cottonseed meal, wheat bran, wheat flour, calcium dihydrogen phosphate, multi-vitamin premix, multi-mineral premix, soybean oil, soybean lecithin, chitosan oligosaccharide, astragalus polysaccharide and choline chloride. The preparation comprises the following steps of: respectively crushing and screening all the raw materials (except the soybean oil) by a crushing machine, weighing the flour, the calcium dihydrogen phosphate, the multi-vitamin premix, the multi-mineral premix, the soybean lecithin, the chitosan oligosaccharide, the astragalus polysaccharide, the choline chloride and other raw materials according to the mass fraction in the formula and mixing uniformly, further weighing the remaining raw materials, uniformly stirring and mixing, fully uniformly mixing with water, performing extrusion and pelletization by a feed machine, and drying at the temperature of 55-60 DEG C to prepare the feed for the tilapia. The compound feed has balanced nutrition, is suitable for requirements of healthy growth of the tilapia and can further reduce the accumulation of fat in livers of the tilapia and improve disease resistance of the tilapia.

The invention discloses a high-sugar and low-protein tilapia feed. Based on the total mass fraction of 100%, it consists of the following components: fish meal: 0-3%, soybean meal: 16-20%, rapeseed meal: 18-21%, cotton meal: 12-16%, secondary meal: 39- 42%, soybean oil: 2-3%, choline chloride: 0.2-0.5%, sodium chloride: 0.1-0.5%, calcium dihydrogen phosphate: 1-2%, vitamin B1: 0.004-0.006%, vitamin B6: 0.002-0.004%, zinc sulfate: 0.02-0.04%, chromium chloride: 0.0001-0.0003%, vitamin premix: 0.1-0.2% and mineral premix: 0.5-1%. The invention maximizes the amount of secondary powder in the feed, adds several plant protein sources to overcome the problem of amino acid imbalance of a single protein source, and at the same time significantly reduces the amount of fish meal. By optimizing and adjusting the feed formula, supplementing suitable vitamin and mineral premixes to adjust the sugar utilization ability of tilapia, so that the tilapia can make good use of the sugar compound of the present invention, avoiding the damage of fish liver caused by high sugar, Fatty liver is formed, and even the fish body becomes obese, and the symptoms of "big belly" appear.

Disclosed is a puffed feed for Tilapia and process for preparation, wherein the constituents include (by weight percent) fish flour 8-12%, soybean 20-30%, meat and bones meal 0-10%, rapeseed waste 20-30%, malt 5-10%, liquid phosphatide 0.5-1%, short flour 3-5%, maize 15-30%, calcium hydrogen phosphate 0.5-1%, composite premixed material 1-2%, soybean oil 0-0.5%, fish oil 0.5-1%.

Certain farmed and/or breeding fish such as tilapia are filleted after the fish have been beheaded, slaughtered and had their ventral cavity opened. Cuts are made in the dorsal region and on the ventral side in the caudal region for exposing the dorsal spokes and ventral spokes, respectively. Then, separating cuts are made over the vertebral column connecting the dorsal and ventral cuts. Before peeling separation in the region of the ventral cavity, the ventral lobes are trimmed by separation of the ends of their ventral lobe portions projecting beyond the ribs. Peeling separation is completed by peeling cuts adapted to the rib contour and made with at least two degrees of freedom.

The invention relates to a feed additive specially used for tilapia. The feed additive is prepared by grinding and mixing the following raw materials in parts by weight: 5-8 parts of rhizoma anemarrhenae, 5-8 parts of catalpa leaves, 5-8 parts of the fruit of Chinese wolfberry, 4-7 parts of rheum officinale, 3-7 parts of cortex magnoliae officinalis, 3-6 parts of agastache rugosus, 3-5 parts of the root of red-rooted salvia, 2-4 parts of radix astragali, 1-2 parts of platycladi seeds, 1-2 parts of acalypha australis, 1-2 parts of bighead atractylodes rhizome and 10-25 parts of a carrier. The feed additive specially used for the tilapia obviously improves the economic benefit of tilapia aquaculture, remarkably improves the anti-stress capability of the tilapia, improves the immunocompetence of the tilapia and reduces the occurrence of nutritional fatty liver disease and streptococcicosis in an aquaculture process, so that the survival rate of the tilapia is increased by 15-22%; and furthermore, the feed additive can promote the growth of the tilapia, and improves the quality of the tilapia meat.

The invention discloses a culture method of Tilapia. The culture method comprises the following steps of: selecting a pond which is 2 meters deep, and disinfecting the pond with unslaked lime after emptying the pond in late October every year; before late November, putting grass carp fries which are all heavier than 250g and feeding with a floating grass carp granulated feed when the water temperature is higher than 15 DEG C; in the next year, putting overwintering Tilapia fries which are all heavier than 200g when the water temperature of the pond is higher than 18 DEG C steadily, and feeding the Tilapia fries twice every day; in late July, fishing 90% of Tilapias for sale, then putting current Tilapia fries which are all heavier than 200g, feeding with a Tilapia floating feed containing 28% of protein in a feed ratio of 1-3%; and fishing all the fishes for sale in late October. By utilizing the culture method, the survival rates of Tilapia and grass carp are higher than 99% and 98% respectively, the yearly output of the pond is improved by 15%, and the commercial fishes are large and uniform in specification and are good in quality. The culture method is a healthy ecological culture mode mainly for culturing Tilapia.

The invention provides a production method for purified recombinant proteins of commercial value prepared in enclosed aquatic systems. The production system provides biosecure containment of genetically engineered aquatic organisms for an alternative production method for complex proteins requiring post-translational modification that is best done in eukaryotic expression systems. Possible aquatic organisms that can be utilized by this invention are crustaceans (e.g. penaeid shrimp or Artemia) and teleost fish (e.g. trout or tilapia).

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.

The invention relates to a method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide and belongs to the technical field of living fish waterless transportation. According to the method, the carbon dioxide serves as the anesthetic of the tilapia mossambica, the influences of the concentration of the carbon dioxide, the temperature of narcotizing liquid, the narcotizing time, the keep-alive temperature, oxygenation and other parameters on the waterless keep-alive time of the tilapia mossambica are studied, and the optimal tilapia mossambica waterless keep-alive transportation parameters are determined. After the method is applied, the tilapia mossambica can be kept alive for four to five hours, and the survival rate is 100 percent. According to the method, the transportation efficiency is improved, the transportation cost is reduced, and meanwhile the transported tilapia mossambica does not have the potential safety hazard of medicine residues.

The invention relates to special compound feed for tilapia, which is prepared by the following raw materials in parts by weight: 3-8 parts of fish meal, 15-30 parts of soybean meal, 15-30 parts of rapeseed meal, 10-15 parts of cottonseed meal, 10-15 parts of DDSG (Distillers Dried Grains with Solubles), 10-20 parts of flour, 10-12 parts of corn, 4-8 parts of zeolite powder, 1-3 parts of soybean oil, 1-2 parts of fish oil, and 0.6-1.2 parts of special feed additive for the tilapia, and the sum of the parts by weight of the raw materials is 100 parts. The special compound feed for the tilapia can improve the economic benefit of tilapia culture distinctly, enhance the anti-stress capacity of the tilapia significantly, improve the immunity of the tilapia, and reduce the incidences of nutritive fatty liver diseases and streptococcicosis during the culture; a survival rate of the tilapia is increased by 16-20%; the growth of the tilapia is promoted; and the quality of fish is improved.

The invention discloses recombinant oral protein TAT-GH of tilapia, a preparation method for the recombinant oral protein TAT-GH and application of the recombinant oral protein TAT-GH. The method comprises the following steps of: extracting total messenger ribonucleic acid (mRNA) from a pituitary gland of male tilapia, performing reverse transcription-polymerase chain reaction (RT-PCR) to obtain complementary deoxyribonucleic acid (cDNA), performing PCR amplification to obtain a growth hormone (GH) gene of the tilapia, and amplifying a TAT-GH gene by a PCR overlap extension method; constructing a recombinant plasmid pET-32a(+)-TAT-GH; and transforming Escherichia coli BL21 (DE3) by using the recombinant plasmid pET-32a(+)-TAT-GH, efficiently expressing fusion protein TAT-GH in the form of an inclusion body under the induction of isopropyl thiogalactoside (IPTG), and purifying and renaturing to obtain active protein. The protein can effectively promote the growth and development of fries of the tilapia after being fed to the fries of the tilapia.

The invention relates to a method for extracting fishskin collagen polypeptide by taking the wastes of such fishes as codfish, salmon or tilapia and the like as the raw materials, which comprises the processing steps of extracting single-spiral collagen, removing fishy smell and deodorizing, enzyme cutting, decolourization, desalting and spray drying; the fishskin containing natural collagen of triplehelix tight structure is washed, drained and then added into softened water for being extracted for three hours by controlling the pH value and the temperature condition, then the pH value is adjusted again and diatomite is added for coarse filtration, and the coarse filtrate is the single-spiral fishskin collagen; and the coarse filtrate is carried out decompress concentration and added with organic solvent for being mixed and stirred for 1 hour, and then rest, extracted and carried out fishy smell removing and deodorizing; and then an enzyme mixture is added to enzyme-cut the solution and a decoloring agent is also added for decoloring for 0.5 hour when the temperature control and stirring are carried out, the solution is then filtered; and finally, desalting and spray drying are carried out to obtain white fishskin collagen polypeptide powder products. The method has simple technical process, convenient operation and control, high utility ratio and good quality.

The invention belongs to the field of fish larvae cultivation, and particularly relates to an application of fishery electrochemical water in transporting fish larvae of tilapia mossambica. The safe and environment-friendly fishery electrochemical water, which is without any pollution, bacteria, algae and insects, is used for transporting the fish larvae, and is used for transporting the famous fish larvae, such as the tilapia mossambica, so that the transportation survival rate and quality of the fish larvae can be effectively improved, the transportation time is beneficially prolonged, and the transportation capability is beneficially improved. The application and a method provided by the invention are simple and convenient in operation, become effective quickly, and have a wide application prospect.

The invention discloses a feed applicable to industrial aquaculture of tilapia, and a preparation method and a feeding method of the feed. The feed is prepared from corn protein powder, chicken powder, bean pulp, fermented rapeseed meal, fermented cottonseed meal, flour, soybean oil, fish oil, rice bran, mineral substances, vitamins and the like according to certain weight percent. The materials are prepared into granules by the steps of mixing, crushing, pelletizing, drying, screening and the like, and the granules are fed to the industrially cultivated tilapia according to the corresponding feeding method. The feed disclosed by the invention is made from the common materials, is low in cost, and is made by the simple and reliable preparation method; the raw materials are reasonable in proportion, and thus the feed can better meet the physiological needs of the industrially cultivated tilapia; the excrement of the tilapia is good in molding and unlikely to scatter, thus being discharged out of a culture system more easily. By virtue of the feeding method disclosed by the invention, ammonia and nitrogen emission can be reduced, the content of ammonia nitrogen and nitrite in a water body can be effectively reduced, and the resistance of the tilapia to streptococcus is enhanced.