305 results about "Tilapia sp." patented technology

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for identifying streptococcicosis resistance of tilapia and application of the SNP site combination. DNAs of tilapia tail fin tissues attacked by streptococcus agalactiae are re-sequenced, resistant and sensitive individuals of a tilapia population are screened, GWAS analysis is carried out, SNPs sites related to tilapia streptococcus agalactiae disease resistance are identified, functional gene annotation and enrichment analysis are carried out, two SNP sites LG1320881657 and LG1320548718 are screened, and the SNP sites related to tilapia streptococcus agalactiae disease resistance are obtained. The GG genotype of the LG1320881657 and the TT genotype of the LG1320548718 are the dominant genotypes related to the streptococcus agalactiae disease resistance of the tilapia mossambica. The tilapia mossambica resistant to streptococcosis is successfully screened by utilizing the two SNPs sites, effective SNPs are provided for developing tilapia mossambica anti-streptococcosis molecular marker-assisted breeding, excellent characters of parents are ensured, and the breeding efficiency is improved.

The invention discloses a method for targeted creation of a fast-growing new tilapia strain, and belongs to the technical field of fish cultivation. The method specifically comprises the following steps: (1) taking a fragment, as shown in SEQ ID NO.1, on a third exon of a pomc gene as a gRNA target sequence; (2) designing and synthesizing a gRNA primer sequence; (3) amplifying by adopting the gRNA primer sequence and the gRNA plasmid, and then transcribing and purifying to obtain a gRNA fragment; (4) transferring the gRNA fragments into fertilized eggs of tilapia mossambica through gene editing to obtain F0-generation positive fish; and (5) hybridizing the F0-generation positive male fish with the wild-type female fish, screening out F1-generation parent fish, and culturing the F1-generation parent fish to obtain F2-generation tilapia with homozygous deletion mutation. According to the invention, the pomc homozygous knockout line is established in the Nile tilapia mossambica for the first time, the new fast-growing line is a complete gene function deletion model, the cost is lower, the efficiency is higher, and the phenotype can be stably inherited.

The invention particularly relates to a method for extracting ACE (Angiotensin Converting Enzyme) inhibitory peptide from tilapia skin. The tilapia skin is a by-product rich in collagen, and has important development significance as a screening raw material of the ACE inhibitor. The invention provides a method for extracting an ACE (Angiotensin Converting Enzyme) inhibitor by taking tilapia skin as a raw material, which comprises the following steps of: degreasing the tilapia skin, crushing, adding acid liquor, extracting to obtain fish skin collagen crude protein, adding enzyme into the fish skin collagen crude protein for enzymolysis, and reserving the part with the molecular weight of less than 3kDa in an enzymolysis product, namely the ACE inhibitory peptide. Particularly, the enzyme is a product of bacillus subtilis or bacillus coagulans under gelatin-induced fermentation.

The invention provides a primer for identifying tilapia mossambica as well as a detection kit and application thereof, and belongs to the technical field of molecular biology. The invention provides a primer for identifying the variety of tilapia mossambica, which can be used for quickly, efficiently and accurately identifying Oreochromis niloticus, Oreochromis aurea and Oreochromis spp., so that a scientific basis is provided for germplasm resource protection and variety identification of the tilapia mossambica.

The invention relates to the field of food technology, and discloses a phosphorus-free frozen water-retaining agent for tilapia fillets, and a preparation method and application thereof. A phosphorus-free frozen water-retaining agent for tilapia fillets is composed of the following substances in mass fractions: 0.3-0.7% sorbitol, 0.1-0.5% sodium citrate, 0.5%-1.3% soy protein isolate, and the rest is distilled water. The preparation method of the phosphorus-free frozen water-retaining agent is to take sorbitol, sodium citrate, and soy protein isolate in a container respectively, add distilled water, stir and dissolve to obtain. The phosphorus-free frozen storage method of tilapia fillets is to take the tilapia fillets and soak them in the water-retaining agent solution for 0.5-1.5 hours, take them out and vacuum pack them, and then quickly put them into a -20°C environment for frozen storage. The phosphorus-free frozen water-retaining agent of the present invention can not only inhibit the denaturation of protein and fat of tilapia during frozen storage, maintain the stability of fish fillet protein, but also improve the texture characteristics of the fish fillets, and improve the antifreeze and water retention.

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention relates to the technical field of gene engineering, and particularly discloses streptococcus agalactiae WC1535 delta cps delta hylB as well as a construction method and application thereof. The streptococcus agalactiae WC1535 [delta] cps [delta] hylB is preserved in Guangdong Microbial Culture Collection Center on February 28, 2025, the preservation number is GDMCC NO.65955, and the address of the preservation unit is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Compared with a traditional single-fragment deletion method, the toxicity of the deletion strain is not prone to being enhanced, and compared with a traditional injection immune vaccine, the gene deletion strain is used for preparing the tilapia oral live vaccine, the immune operation is simpler, and more labor cost is saved.

The invention belongs to the technical field of bacterial infection reagents, and particularly relates to application of a reagent for detecting markers in tilapia in preparation of a detection product for detecting tilapia streptococcus agalactiae infection. According to the application disclosed by the invention, the expression levels of Hap and IL-6 in blood and Hep and IL-6 in spleen of the streptococcus agalactiae infected tilapia mossambica are more obviously changed compared with that of the tilapia mossambica which is not infected by the streptococcus agalactiae; whether tilapia is infected with streptococcus agalactiae or not can be accurately judged according to expression levels of Hap in tilapia blood, IL-6 in tilapia blood, Hep in tilapia spleen and IL-6 in tilapia spleen.

The invention provides collagen tripeptide as well as a preparation method and application thereof, and belongs to the technical field of active peptide. The invention provides a preparation method of collagen tripeptide, which comprises the following steps: carrying out enzymolysis treatment on tilapia scales and high-temperature-resistant protease, then carrying out enzymolysis treatment by using alkaline protease and neutral protease in sequence, filtering enzymatic hydrolysate by using a ceramic membrane and an organic filter membrane in sequence, and intercepting by using a 400Da nanofiltration membrane to obtain collagen tripeptide. The permeate contains active peptide mainly composed of collagen tripeptide. After the permeate is concentrated and dried, the collagen tripeptide powder is detected, and the protein content reaches 96.2%, the GPH content is 3.8%, and the CTP content is 35.09%. The collagen tripeptide powder has better DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity; the traditional Chinese medicine composition has an obvious ROS removing effect, a beta-galactosidase activity inhibiting effect and a telomerase activity enhancing promoting effect on a hydrogen peroxide induced zebrafish aging model, and shows an obvious anti-aging effect.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention belongs to the field of computer vision, and provides a tilapia behavior image detection method, system and equipment, and the method comprises the steps: enabling the fish mouth feature vectors of a plurality of samples to form a feature matrix, generating a fish mouth feature alignment index matrix, and extracting a target alignment vector and a reference alignment vector; and respectively calculating the inner product of the alignment index vector of each sample, the target alignment vector and the reference alignment vector to obtain a target alignment score and a reference alignment score, taking the distance between the target alignment score and the reference alignment score as a behavior offset index, and mapping the behavior offset index as the probability of the parent hatch behavior. The method has natural robustness, can restrain the influence of accidental noise, can effectively recognize the female incubation behavior in the underwater complex environment, and has higher real-time response capability in actual aquaculture monitoring.

The invention relates to a tilapia fillet trimming track generation method based on image recognition, and relates to the technical field of aquatic product processing, and the tilapia fillet trimming track generation method comprises the following steps: image data pre-processing, fish back burr trimming track, fish tail trimming track, fish belly trimming track and fish head side part trimming and spine removal V-opening track. According to the method, each independent fish fillet has different shapes and contours, and an exclusive trimming track is planned and customized through visual sampling.

The invention relates to the technical field of biology, in particular to a detection method for tilapia streptococcus agalactiae resistance, a KASP primer group and application. According to the invention, the key SNP site of the ABCB4 gene, which is obviously associated with the streptococcus agalactiae disease resistance, of the tilapia is identified by a population genetics method, a typing method of the SNP site is developed by a KASP technology, efficient evaluation on disease resistance characters is realized, experimental data shows that the survival time of an AA genotype individual in a challenge test is prolonged compared with that of a GA / GG genotype individual, and the survival time of the AA genotype individual in the challenge test is obviously prolonged compared with that of a GA / GG genotype individual. The proportion of resistant fishes is remarkably increased, and genotype frequency distribution is strongly related to group disease resistance. According to the breeding strategy based on the SNP marker, the disease resistance of bred groups can be integrally improved, the morbidity and death rate are reduced, an efficient genetic improvement tool is provided for breeding of tilapia mossambica for resisting streptococcus agalactiae diseases, the loss of breeding diseases is reduced, and antibiotic dependence is reduced.

The invention provides a fresh tilapia mossambica block preservative and a preparation method thereof, the fresh tilapia mossambica block preservative comprises the following raw materials: natural plant extract, modified biological polysaccharide, organic acid, natural antibacterial peptide, a humectant, deionized water and the like, and propolis extract and astaxanthin can be selected to enhance the effect; wherein the natural plant extract is corncob, zingiber mioga and tartary buckwheat bran extract; the modified biological polysaccharide is obtained by modifying chitosan quaternary ammonium salt, laminarin, cell wall polysaccharide and carboxylic acid. The fresh-keeping agent is prepared through ultrasonic mixing, multilayer electrostatic deposition and other technologies, through the synergistic effect of all the raw materials, a multilayer coating structure is formed through electrostatic deposition, microbial growth can be inhibited, gas exchange can be controlled, water loss can be reduced, the fresh-keeping period of tilapia mossambica blocks can be effectively prolonged, and the quality of the tilapia mossambica blocks can be kept.

The invention discloses a tilapia skin DPP-IV inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of food-borne active peptide development. The preparation method comprises the following steps: taking tilapia skin as a raw material, extracting gelatin from the tilapia skin through an acid-alkali treatment method, and then performing simulated gastrointestinal digestion on the gelatin by utilizing simulated digestive juice prepared from pepsin, trypsin and chymotrypsin to obtain a digestion product with DPP-IV inhibitory activity; the tilapia skin active peptide with relatively high DPP-IV inhibitory activity is obtained through further ultrafiltration and identification, amino acid sequences of the tilapia skin active peptide are AKVELLK, VPGPMGPMGPR and DGIR respectively, and the three inhibitory peptides have good DPP-IV inhibitory activity and can be used for developing food, health care products or medicines for assisting in regulating blood sugar.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) marker combination for identifying tilapia streptococcus agalactiae disease resistance and application thereof. The invention discloses 11 SNP loci significantly related to streptococcus agalactiae resistance in a tilapia UTRN gene, and constructs 18 SNP marker combinations for streptococcus agalactiae resistance phenotype identification, so that the resistance phenotype identification accuracy and hereditary stability can be effectively improved. Besides, according to the SNP typing primer and method based on the # imgabs0 # MALDI-TOF mass spectrum platform, high-throughput and high-precision genetic typing can be achieved, and the SNP typing primer and method have important application value in resistance phenotype identification and assistant selective breeding.

The invention relates to the technical field of biology, in particular to a tilapia mossambica streptococcus agalactiae resistance related KASP molecular marker and application thereof. According to the invention, two SNP (Single Nucleotide Polymorphism) loci, namely SNP-1 and SNP-2, which are remarkably associated with resistance are screened based on an intron region of an EGFRX2 gene of tilapia mossambica, a typing method of the loci is developed through a KASP technology, and relevance between the genotype of the SNP-1 and a resistance phenotype is verified. Furthermore, streptococcus agalactiae resistance dominant diplotype individuals are screened in combination with double SNP loci, and the survival rate of a challenge experiment is remarkably improved. The detection kit provided by the invention can realize high-throughput and high-precision typing, is suitable for tilapia mossambica resistance genotype identification and molecular marker-assisted breeding, provides an efficient genetic improvement tool for tilapia mossambica disease-resistant breeding, and is beneficial to reduction of breeding disease loss and reduction of antibiotic dependence.

The invention belongs to the technical field of biology, and particularly relates to tilapia skin peptide, a preparation method thereof and application of the tilapia skin peptide in preparation of anti-aging related products. The tilapia skin peptide disclosed by the invention is IL, FGP or LPGL. In the invention, the tilapia skin is subjected to enzymolysis to prepare protein peptide, a peptide fragment with high content and high reliability is selected through mass spectrum identification, and a pure product is prepared by using a solid-phase chemical synthesis method. And by taking GPH as a positive control group, IL, FGP and LPGL are found to have a protection effect on UV-induced cell injury, have good antioxidant activity and an effect of repairing injured cells, and are beneficial to protecting a skin barrier and delaying skin aging.

The invention discloses bacillus subtilis B-1124 and application thereof in aquaculture, and belongs to the technical field of microorganisms. The bacillus subtilis B-1124 is screened from the bottom mud of the aquaculture water body, after domestication culture, the bacillus subtilis B-1124 can better adapt to the environment and can stably exist in the relatively harsh environment, and the bacillus subtilis B-1124 with the high spore yield is suitable for production, improves the survival rate of final probiotics and meanwhile has a more excellent antioxidant effect. When the bacillus subtilis B-1124 or the culture of the bacillus subtilis B-1124 is applied to the aquaculture industry, the bacillus subtilis B-1124 or the culture of the bacillus subtilis B-1124 is free of obvious toxic and side effects, the feed conversion rate of aquatic animals is increased, the intestinal oxidative stress level can be obviously improved, the generation of inflammatory factors is reduced, the ecological disorder of intestinal flora can be relieved, and the immunity of the organism can be improved; and the disease resistance of the body to streptococcus agalactiae is improved, so that the death rate of the tilapia is reduced.

The invention discloses a tilapia mossambica processing device which comprises a workbench, a packaging box is attached to the top of the workbench, a baffle is fixedly connected to the position, close to the left side, of the top of the workbench, two conical blocks are fixedly connected to the bottom of the workbench, the two conical blocks are arranged in a bilateral symmetry mode, and two connecting plates are arranged at the bottom of the workbench; through mutual cooperation of the component workbench, the L-shaped fixing plate, the baffles, the connecting plates, a transverse rod, a transverse plate, a swing plate, an attaching plate, a stabilizing plate, a movable plate, a bottom plate, a balance plate, a balancing weight, a linkage rod, a hinge block and other components, a user can firstly adjust the position of the balancing weight; and when the tilapia mossambica is added into the packaging box, the balance plate can be horizontally stable, so that the weight of the tilapia mossambica in the packaging box is consistent.

The invention relates to the technical field of collagen peptide preparation, and discloses a collagen peptide as well as a preparation method and application thereof. The preparation method of the collagen peptide comprises the following steps: adding water into Hefang crucian carp skin, regulating the Hefang crucian carp skin into feed liquid with the optimal pH value of protease, then adding the protease into the feed liquid for enzymolysis, inactivating after enzymolysis, and centrifuging to take supernate, so as to obtain the collagen peptide. The protease comprises one or more than two of alkaline protease, animal protease and neutral protease. According to the present invention, the He-prescription crucian carp skin is adopted as the preparation raw material, the protease most suitable for the raw material is adopted to perform enzymolysis to prepare the collagen peptide, the optimal enzymolysis process parameters are determined, and compared with the conventional tilapia skin collagen peptide, Hunan crucian carp skin collagen peptide and collagen tripeptide in the antioxidant function, the antioxidant activity is significantly improved, and the antioxidant activity is significantly improved; therefore, the method for preparing the crucian carp skin collagen peptide with remarkable antioxidant activity is established, and the application range of crucian carp processing byproducts is further expanded.

The invention belongs to the field of tilapia mossambica breeding, and discloses a rapid and efficient tilapia mossambica breeding method and system.The method comprises the steps that tilapia mossambica populations with excellent character potential are collected and screened, and a basic breeding population is established; molecular markers related to target characters are developed, and individuals carrying excellent genes are rapidly screened; a target gene is precisely regulated and controlled through a CRISPR / Cas9 technology, so that stable inheritance of excellent characters is accelerated; phenotypes such as growth performance, disease resistance and environment resistance are efficiently evaluated and screened; the excellent characters are fixed; and the performance of the new strain under the actual breeding condition is evaluated. The efficient and low-cost tilapia mossambica breeding method is constructed by integrating molecular marker, gene editing and phenotype screening technologies, the breeding efficiency and the commercialization value of a new strain are remarkably improved, and powerful technical support is provided for the aquaculture industry.

The invention relates to the technical field of genomics and metabonomics, in particular to a method for analyzing intestinal health of crisp tilapia mossambica. The tilapia mossambica intestinal health judgment model is established by dynamically monitoring tilapia mossambica muscle texture and intestinal morphology through high time resolution, analyzing intestinal health three-dimensional indexes (tissue morphology-flora structure-host gene expression) and serum metabolism response after embrittlement succeeds, and combining cross-omics data association analysis. The change condition of the muscle texture can be known more timely, a more accurate basis is provided for adjustment of a feeding strategy, and the stability of the fish meat quality can be improved. The intestinal health is evaluated from three dimensions of histomorphology, flora structure and host gene expression, so that the health condition of the intestinal tract can be known more comprehensively, and more comprehensive reference is provided for optimization of a feeding method. The correlation between different omics data can be deeply analyzed, the physiological and metabolic mechanisms of the fish body are disclosed, and deeper theoretical support is provided for optimization of a feeding method.

The application discloses a hybridoma cell strain secreting an anti-tilapia interleukin-2 monoclonal antibody and an application thereof, and the hybridoma cell strain 1C11F5 is preserved in the China Center for Type Culture Collection (CCTCC) on March 9, 2024, and the preservation number is CCTCC NO: C202470. The application can specifically identify the tilapia IL-2 protein and the lymphocyte group secreting IL-2, is helpful for further exploration of T cell activation and proliferation, and provides an important immunological tool for adaptive immune mechanism research of hard fish (such as tilapia and the like).

The invention provides a crispness grading method for crisp tilapia, and relates to the technical field of tilapia quality detection. The method for grading the crispness of the crisp tilapia mossambica comprises the following steps: determining the crispness of the raw crisp tilapia mossambica by adopting a texture analyzer, setting a sensory evaluation crispness grade for the cooked crisp tilapia mossambica, simultaneously performing sensory evaluation, and performing correlation analysis on the crispness value of the raw crisp tilapia mossambica and the sensory score of the cooked crisp tilapia mossambica. Therefore, the crispness value of the raw crisp-meat tilapia and the grading contrast condition of the cooked crisp-meat tilapia are set. According to the method, the defects in the prior art are overcome, and the sensory evaluation crispness grade of the cooked crisp tilapia can be predicted according to the meat crispness detection result of the raw crisp tilapia, so that reliable reference is provided for transaction pricing, breeding process crispness control, commodity grading and processing process quality control of the crisp tilapia.

The utility model discloses a thawing device of tilapia processing, including support frame still including the deflector that makes fish body slide and the deflector of surface of installation in support frame, the baffle of fixed connection on deflector, the intercepting assembly of installation on baffle and intercept fish body, thawing assembly of installation on baffle, the liquid collecting assembly of installation on support frame and the guide assembly of installation on liquid collecting assembly, fish body moves on deflector, and thawing assembly is used for spraying water to fish body, and the wastewater of thawing is collected through liquid collecting assembly, and the fish body of thawing is finished and passes through the guide assembly empty part moisture while from the liquid collecting assembly top. The deflector of ladder form makes fish body slide under the action of gravity and turns over naturally, ensures that each surface of fish body can evenly receive thawing water, effectively eliminates "thawing dead angle", avoids the forced turning of rigid parts such as mechanical arm, prong in whole process, maximum degree reduces muscle tissue damage, and can guarantee the continuity of thawing process.

1. Name of the product in this design: Fish bait (imitating tilapia). 2. Purpose of this design: Fishing tackle for fishing. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The utility model relates to the field of tilapia mossambica cleaning devices, and discloses a tilapia mossambica processing and cleaning device which comprises a fixing support, a cleaning shell is fixedly mounted in the fixing support, two partition plates are fixedly mounted in the cleaning shell, and a plurality of water permeable holes are formed in the top surfaces of the two partition plates; an exhaust shell is fixedly installed at the position, below the partition plate, in the cleaning shell, a plurality of exhaust nozzles corresponding to the water permeable holes are fixedly installed on the top face of the exhaust shell, and compressed air generated by an external air pump is guided into the exhaust shell through an air guide pipe and sprayed out through the exhaust nozzles; the jetted air forms upward airflow in the cleaning shell, so that the fillets soaked in the clear water roll over, impurities and blood attached to the surfaces of the fillets can be effectively removed through rolling over, the cleaning effect is remarkably improved, and sanitation and quality of the fillets in the cleaning process are guaranteed.

The present application relates to the field of biotechnology, and particularly relates to a KASP molecular marker related to the resistance of tilapia to streptococcus agalactiae disease and an application thereof.Two SNP sites (SNP-1 and SNP-2) significantly associated with the resistance are screened from the intron region of EGFRX2 gene of tilapia, a typing method of the above sites is developed through KASP technology, and the correlation between the SNP-1 genotype and the resistance phenotype is verified.Further, an advantage haplotype individual with the resistance to streptococcus agalactiae disease is screened by combining the two SNP sites, and the survival rate of the individual in the challenge experiment is significantly improved.The detection kit provided by the present application can realize high-throughput and high-precision typing, is suitable for the identification of the resistance genotype of tilapia and the marker-assisted selection, provides an efficient genetic improvement tool for the disease-resistant breeding of tilapia, and is helpful to reduce the loss of breeding diseases and reduce the dependence on antibiotics.