117 results about "Tilapia sp." patented technology

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention belongs to the field of computer vision, and provides a tilapia behavior image detection method, system and equipment, and the method comprises the steps: enabling the fish mouth feature vectors of a plurality of samples to form a feature matrix, generating a fish mouth feature alignment index matrix, and extracting a target alignment vector and a reference alignment vector; and respectively calculating the inner product of the alignment index vector of each sample, the target alignment vector and the reference alignment vector to obtain a target alignment score and a reference alignment score, taking the distance between the target alignment score and the reference alignment score as a behavior offset index, and mapping the behavior offset index as the probability of the parent hatch behavior. The method has natural robustness, can restrain the influence of accidental noise, can effectively recognize the female incubation behavior in the underwater complex environment, and has higher real-time response capability in actual aquaculture monitoring.

The application discloses a hybridoma cell strain secreting an anti-tilapia interleukin-2 monoclonal antibody and an application thereof, and the hybridoma cell strain 1C11F5 is preserved in the China Center for Type Culture Collection (CCTCC) on March 9, 2024, and the preservation number is CCTCC NO: C202470. The application can specifically identify the tilapia IL-2 protein and the lymphocyte group secreting IL-2, is helpful for further exploration of T cell activation and proliferation, and provides an important immunological tool for adaptive immune mechanism research of hard fish (such as tilapia and the like).

The utility model discloses a thawing device of tilapia processing, including support frame still including the deflector that makes fish body slide and the deflector of surface of installation in support frame, the baffle of fixed connection on deflector, the intercepting assembly of installation on baffle and intercept fish body, thawing assembly of installation on baffle, the liquid collecting assembly of installation on support frame and the guide assembly of installation on liquid collecting assembly, fish body moves on deflector, and thawing assembly is used for spraying water to fish body, and the wastewater of thawing is collected through liquid collecting assembly, and the fish body of thawing is finished and passes through the guide assembly empty part moisture while from the liquid collecting assembly top. The deflector of ladder form makes fish body slide under the action of gravity and turns over naturally, ensures that each surface of fish body can evenly receive thawing water, effectively eliminates "thawing dead angle", avoids the forced turning of rigid parts such as mechanical arm, prong in whole process, maximum degree reduces muscle tissue damage, and can guarantee the continuity of thawing process.

The present application relates to the field of biotechnology, and particularly relates to a KASP molecular marker related to the resistance of tilapia to streptococcus agalactiae disease and an application thereof.Two SNP sites (SNP-1 and SNP-2) significantly associated with the resistance are screened from the intron region of EGFRX2 gene of tilapia, a typing method of the above sites is developed through KASP technology, and the correlation between the SNP-1 genotype and the resistance phenotype is verified.Further, an advantage haplotype individual with the resistance to streptococcus agalactiae disease is screened by combining the two SNP sites, and the survival rate of the individual in the challenge experiment is significantly improved.The detection kit provided by the present application can realize high-throughput and high-precision typing, is suitable for the identification of the resistance genotype of tilapia and the marker-assisted selection, provides an efficient genetic improvement tool for the disease-resistant breeding of tilapia, and is helpful to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The present application belongs to the technical field of food processing, and relates to a method for removing earthy smell of tilapia fillets. The method comprises the following steps: covering the surface of the tilapia fillets with pleurotus eryngii powder, and placing the tilapia fillets in a deodorizing and fresh-keeping liquid for 30-60 min; the deodorizing and fresh-keeping liquid is composed of ginger powder 15-20 parts, cannabis seed protein 5-10 parts, lysozyme 0.5-1 part, tea polyphenol 4-8 parts and water 60-100 parts in terms of mass fraction. The present application solves the technical problem of long-term maintenance of fresh fish flavor when removing the earthy smell of tilapia fillets.

The present invention relates to the effect of promoting muscle development and growth through inhibition of the expression of MRF4. According to the present invention, MRF4 isolated and identified from skeletal muscles of Nile tilapia showed a strong association with MyoG and MEF2 proteins, and the expression of MyoG and MEF2 was found to significantly increase when the MRF4 gene was disrupted through CRISPR / Cas9 gene editing, and thus the present invention has the effect of being able to be used for the promotion of muscle development or the treatment of muscle diseases through the inhibition of the MRF4 gene.

The invention discloses a tilapia scaling quality classification method and system based on hyperspectrum and machine learning, and the method comprises the steps: obtaining the hyperspectral information of tilapia with different scaling qualities, carrying out the spectrum preprocessing, and obtaining the hyperspectral data without noise and background; obtaining a hyperspectral data set of the tilapia mossambica with different scaling qualities; constructing a tilapia scaling quality identification model based on a convolutional neural network and an SVM algorithm, inputting the hyperspectral data sets of various tilapia with different scaling qualities into the model for training, and obtaining an optimal model through training; hyperspectral information of to-be-detected scaled tilapia mossambica is obtained and input into the optimal model for detection, and according to an identification result, the classification device classifies the tilapia mossambica of which the scaling quality does not reach the standard. According to the method, the classification of the tilapia mossambica with unqualified scaling quality can be effectively realized, and the efficiency and the accuracy of the scaling quality detection of the tilapia mossambica are improved to a great extent.

The application discloses a recombinant strain and a preparation method thereof and application of the recombinant strain in preparation of a vaccine for Streptococcus agalactiae of tilapia, and the recombinant strain is successfully constructed by the following steps: designing an ORF4 primer, amplifying an ORF4 gene fragment from a DNA template of Streptococcus agalactiae, editing the ORF4 gene fragment into Escherichia coli by means of a CRISPR / Cas9 gene editing technology, and connecting a green fluorescent protein (GFP). The recombinant strain is subjected to immunoprotection research on the Streptococcus agalactiae of tilapia, and it is shown that the recombinant strain has a good protection effect, and can provide a solid theoretical basis and technical support for application and development of the vaccine for Streptococcus agalactiae of tilapia in the field of aquaculture.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

The application discloses a strain of Sideropenaeus paramamosain bio-floc high-pond Shajia external sulfur red spirillum HDES1 and application of the strain in purifying inorganic nitrogen and phosphorus in aquaculture water, and belongs to the technical field of microorganisms. The Shajia external sulfur red spirillum HDES1 in the application is screened from a Sideropenaeus paramamosain bio-floc aquaculture high-pond, and has a preservation number of CCTCC NO: M 20191007. It is found through experiments that the strain has a remarkable purifying effect on high-concentration inorganic nitrogen and phosphorus in seawater aquaculture water or tail water, has no adverse effect on cultured Sideropenaeus paramamosain and seawater tilapia, and is helpful to improving the yield of fish and shrimp culture and realizing zero-water-change green ecological culture.

The application provides a method for improving the flavor and quality of tilapia surimi products, and belongs to the technical field of aquatic product processing, and comprises the following steps: tilapia muscles are cut into small pieces, an improving agent is added, and the muscles are ground; the well-stirred surimi is made into fish cakes and shaped; the fish cakes are taken out after floating, and are placed in cold water for cooling; the shaped fish cakes are respectively fried at 150 DEG C, 180 DEG C and 210 DEG C for 4 min; the fish cakes are vacuum packaged and stored in a refrigerator after cooling; the flavor of the surimi is analyzed, the quality of the surimi is detected, and the deterioration of the surimi is determined and analyzed; threonine, creatinine and glucose are dissolved in a phosphate buffer to obtain A liquid; diethylene glycol is taken, mixed with soybean oil, and mixed with an improving agent by vortexing to obtain B liquid; the A liquid and the B liquid are fully mixed in a 48 mL sealed high-temperature pressure-resistant reaction bottle, and then are placed in an oil bath for reaction and then are placed in an ice water bath for reaction termination; the solution after reaction is removed and is reserved; the MeIQx content, precursor substances and intermediate substances of the MeIQx generation simulation system solution after reaction simulation are determined.

The present application relates to the field of biotechnology, and more particularly to a detection method for resistance of tilapia to streptococcosis, a KASP primer set and application. The present application identifies a key SNP site of the ABCB4 gene significantly associated with resistance of tilapia to streptococcosis by the method of population genetics, and develops a genotyping method for the SNP site by KASP technology, thereby realizing efficient evaluation of the disease-resistant trait. Experimental data show that the survival time of AA genotype individuals in the challenge test is longer than that of GA / GG genotype individuals, the proportion of resistant fish is significantly improved, and the genotype frequency distribution is strongly correlated with the disease resistance of the population. The breeding strategy based on the SNP marker can improve the overall disease resistance of the breeding population and reduce the mortality rate, thereby providing an efficient genetic improvement tool for breeding of tilapia resistant to streptococcosis, and helping to reduce the loss of breeding diseases and reduce the dependence on antibiotics.

The utility model relates to the technical field of tilapia mossambica quick-freezing, and discloses a tilapia mossambica quick-freezing and sequencing all-in-one machine which comprises a conveying belt, a quick-freezing box is fixedly connected to the side wall of the conveying belt, and a drying assembly is arranged at the top of the conveying belt. According to the tilapia mossambica quick-freezing and sorting all-in-one machine, most surface moisture is rapidly taken away through high-speed airflow, the initial dehumidification efficiency is improved, meanwhile, secondary wetting caused by water vapor condensation is avoided, after the surface moisture is blown away, the amount of residual moisture on the surface of a tilapia mossambica body is greatly reduced, slow drying is conducted at the moment, needed heat energy is less, energy is saved, and the production cost is reduced; the tilapia mossambica is dried through the first cavity and the second cavity on the upper side and the lower side respectively, hot air can act on the upper surface and the lower surface of the tilapia mossambica at the same time, compared with a single-side drying design, water on the surfaces of the tilapia mossambica can be removed more rapidly and more evenly through double-face drying, and therefore the drying time is shortened, and the working efficiency is improved.

This invention discloses an integrated farming method for reducing the incidence of diseases in tilapia, comprising: configuring farming facilities; pre-culturing water and colonizing microorganisms before stocking; phased lighting control; dynamic water quality control and microbial enhancement; acoustic conditioning and feeding optimization; coordinated management of recirculating water and wastewater discharge; and adding a feed additive composed of activated carbon and Bacillus licheniformis to the basic feed, wherein the activated carbon is 100-300 mesh powder and the concentration of Bacillus licheniformis is 10%. 5 -10 10 CFU / g, activated carbon is pre-mixed with sodium alginate aqueous solution and added to the feed, and bacterial solution is sprayed on top; it is prepared and used immediately. This invention systematically integrates multiple environmental stress factors and activated carbon-Bacillus licheniformis composite additives to significantly reduce the incidence of diseases and improve growth performance in tilapia farming.

This application discloses a method for periodically feeding vine tea ultrafine powder in aquaculture and its application. The method includes the following steps: mixing vine tea ultrafine powder into aquatic animal feed at an addition rate of 1% to 8% of the total feed weight, and feeding it using a periodic feeding pattern; the periodic feeding pattern consists of continuous feeding for 7 days, followed by a 7-21 day break, which constitutes one cycle. This application, by pulverizing vine tea to an optimal particle size of 300 mesh, and by optimizing the optimal addition rate of 4% and the periodic feeding strategy of "7 days of continuous use, 14 days of break," fully utilizes the antioxidant and immune-enhancing effects of vine tea while effectively avoiding the potential liver and kidney burden and immunosuppression risks associated with long-term continuous use. Large-scale application trials show that this method achieves a protection rate of 52.91% to 65.14% in various aquatic animals, including crucian carp, largemouth bass, whiteleg shrimp, carp, and tilapia, demonstrating significant economic benefits and promising prospects for industrial application.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for identifying different geographical populations of Nile tilapia and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The invention provides three SNP (Single Nucleotide Polymorphism) molecular markers. In a reference genome (GCF001858045.2 version), a C / T site (marked as SNP chr4-17660871) exists at the 17660871 site of a fourth chromosome, an A / C site (marked as SNP chr7-30426048) exists at the 30426048 site of a seventh chromosome, and an A / G site (marked as SNP chr16-3192004) exists at the 3192004 site of a sixteenth chromosome. As a detection target, the SNP molecular marker can effectively distinguish geographical populations of an Alexander line, an Ismelia line and a Seneigarg line of Nile tilapia. According to the method, stable, specific and accurate germplasm resource identification can be realized.

This invention provides primers, a detection kit, and applications for identifying tilapia, belonging to the field of molecular biology. The invention provides primers for identifying tilapia species, enabling rapid, efficient, and accurate identification of Nile tilapia (Oreochromis niloticus), Oreochromis aureus, and Oreochromis spp., providing a scientific basis for tilapia germplasm resource conservation and species identification.

The invention discloses a trace element premix for improving eggshell quality. The feed additive is prepared from the following raw materials: mulberry leaf powder, schizochytrium limacinum powder, eggplant stems, negundo chastetree fruits, negundo chastetree roots, spartina anglica, moringa seeds, chequer-shaped indocalamus leaf powder, tilapia bone meal, crab shell powder, a complex microbial inoculant, compound vitamins, compound amino acids, iron glycinate, ferrous lactate, zinc methionine, zinc propionate, manganese glycinate, copper glycinate, strontium citrate, cobalt methionine, calcium glutamate, sodium selenite and the like. The premix can be used for processing feed for improving the quality of eggshells, is suitable for later laying period of laying hens, and can improve the laying rate and the quality of the eggshells at the same time.

The invention discloses an FM550 in-vivo enrichment detection method and a risk monitoring method for tilapia, and relates to the technical field of ecological monitoring. The method not only can reflect the actual risk of a flame retardant in the environment, but also can be conveniently applied to aquaculture and ecological risk assessment. The detection method comprises the following steps: obtaining a liver sample and a muscle sample of a target tilapia mossambica, freeze-drying the liver sample and the muscle sample for 72 hours, and crushing and homogenizing 1 g of the liver sample and the muscle sample for later use; the target tilapia mossambica refers to tilapia mossambica which is exposed in an FM550 environment within 56 days; and carrying out solvent extraction, QuEChERS purification treatment and solvent conversion treatment on the crushed and homogenized sample, and then carrying out gas chromatography-mass spectrometry detection to obtain a detection result of FM550 in-vivo enrichment.

The utility model relates to related technical field of cleaning device discloses a kind of tilapia processing fish meat cleaning device, including waste treatment platform and the cleaning device being set on waste treatment platform, the inside of waste treatment platform is provided with mounting groove, and the inside of mounting groove is rotatably installed with multiple conveying rollers, meshed conveyor belt that is adapted for use with conveying roller is provided on the conveying roller, the left side of the front wall of waste treatment platform is provided with servo motor for driving conveying roller and meshed conveyor belt, the inside lower side of mounting groove is provided with conveyor belt cleaning brush that can be cleaned and used to meshed conveyor belt, and conveyor belt cleaning brush is attached to meshed conveyor belt, the left side lower side of the bottom of waste treatment platform is located in the left side of mounting groove and is provided with waste guide bin for guiding filth, to better automatically clean filth, save manpower, increase work efficiency.

The tilapia gill removing and cleaning device comprises a base, a machine frame and a controller, a conveying belt is arranged on the top face of the base, a fixing base is arranged on the conveying belt, a V-shaped groove is formed in the fixing base, fixing clamping plates are oppositely arranged on the inner wall of the fixing base, supports are oppositely arranged on the side faces of the fixing base, and second electric push rods are oppositely arranged on the side faces of the two supports. The second electric push rod is connected with an air box, the two opposite side faces of the air box are provided with suction cups and exhaust valves, the air box communicates with a vacuum pump through a vacuum pipe, the top of the rack is provided with a moving mechanism, the bottom of the moving mechanism is provided with a third electric push rod, the third electric push rod is connected with a lifting plate, and the lifting plate is provided with a water spraying mechanism and a gill smashing mechanism. According to the tilapia mossambica cleaning device, tilapia mossambica can be fixed in the V-shaped groove, gills are opened through the suction cup, the gills are smashed through the gills smashing mechanism through a rotating circular saw and steel bristles, the gills can be washed away through high-speed water flow generated by the water spraying mechanism, the integrity of the tilapia mossambica is kept, and the cleaning effect is improved.

The invention relates to the technical field of internal circulation low-carbon systems, in particular to a mangrove plant acanthus ilicifolius and bostrichthys sinensis pond planting and breeding coupled internal circulation low-carbon system which comprises three biological components, namely mangrove plant acanthus ilicifolius, bostrichthys sinensis and seawater tilapia, and the three biological components coexist in a low-salt seawater environment with the salinity of 5-8 to form a substance circulation chain; according to the method, the tilapia mossambica culture pond serves as a hub, residual feed and excrement of the tilapia mossambica culture pond are conveyed to the acanthus ilicifolius planting area along with circulating water to provide fertility, chippings of mangrove plant acanthus ilicifolius overflow back to the tilapia mossambica culture pond, and therefore the medicinal and edible value of tilapia mossambica is increased, 70-80% of tilapia mossambica meat mixed feed is fed to Nitrogen and phosphorus-rich bottom mud in a tilapia pond cleaned in winter is recycled as acanthus ilicifolius organic fertilizer, so that resource circulation of'feeding trees with fishes and fattening the fishes with the trees' is realized, and input of exogenous pollution is reduced.

This invention discloses a DPP-IV inhibitory peptide from tilapia skin fermented with Aspergillus oryzae, its preparation method, and its applications, belonging to the field of food-derived bioactive peptide development technology. Using tilapia skin as raw material, this invention employs a combined process of acid-base pretreatment, warm water extraction, Aspergillus oryzae fermentation, gel column chromatography, and semi-preparative liquid chromatography to obtain tilapia skin bioactive peptides with high DPP-IV inhibitory activity. The amino acid sequences are AAGVGEF, GIADRMQ, and KPLRLPL, respectively. These three inhibitory peptides exhibit good DPP-IV inhibitory activity and can be used to develop health products or pharmaceuticals for regulating blood sugar.

This invention relates to a plant-based protein feedstuff for tilapia grow-out, comprising a specific composition of Trichanthera gigantea leafmeal, fishmeal, rice bran, cassava starch, fish oil, vitamins and minerals premix, and distilled water. The feedstuff may further comprise preferred ranges for each component. The invention also discloses a process for making the feedstuff, including harvesting and processing Trichanthera gigantea leaves, drying fishmeal, and combining the ingredients to form pellets.

The invention discloses a feed additive for improving pathogen resistance and intestinal injury of high-fat fed fishes and application of the feed additive, and belongs to the technical field of fish feed. The feed additive is indole-3-propionic acid, and the application of the feed additive is that IPA is added into a high-fat feed according to the addition amount of 20-160 mg / kg. Experiments find that IPA with different concentrations can significantly improve the aeromonas hydrophila infection resistance of tilapia fed with high fat, enhance the intestinal immune enzyme activity, improve the intestinal permeability, enhance the intestinal antioxidant ability and relieve intestinal inflammation. The IPA disclosed by the invention can be used as a novel feed additive, can effectively relieve the problems of pathogen resistance reduction and intestinal injury of high-fat fed fishes, and has a good application prospect.

The invention provides a fish feed compound additive based on L-dopamine as well as a preparation method and application thereof, and relates to the technical field of aquaculture, the fish feed compound additive comprises the following components: 0.01-0.05 part of L-dopamine, 45-70 parts of broad bean extract, 10-25 parts of amino acid compound, 2-5 parts of bile acid, 10-20 parts of L-carnitine, 5-10 parts of Chinese herbal medicine composition and 3-8 parts of compound emulsifier. Through the neuroendocrine regulation and control effect of L-dopamine, the L-dopamine, the broad bean extract, the amino acid compound, the bile acid, the Chinese herbal medicine composition, the compound emulsifier and other components generate multiple synergistic effects, and multi-channel precise regulation and control of the tilapia mossambica muscle texture are achieved.

The utility model relates to the technical field of tilapia mossambica cultivation, and provides a tilapia mossambica classification cultivation device which comprises a cultivation box, a water discharging plug is installed at the lower right end of the cultivation box in a clamped mode, the upper left end of the cultivation box is communicated with a water inlet pipe, sliding grooves are formed in the front inner wall and the rear inner wall of the cultivation box, clamping grooves are formed in the sliding grooves at equal intervals, and the clamping grooves are communicated with the water discharging plug. Separation nets are installed on the left and right side walls of the separation plate, a sealing plate is connected to the upper end of the interior of the separation plate in a penetrating mode, water inlet pipes are connected to the upper and lower ends of the adjusting structure in a penetrating mode, the left side of the adjusting structure communicates with a disinfection structure, and the upper end of the sealing plate is of a T-shaped structure. And the height size of the sealing plate is greater than the internal depth size of the partition plate. According to the tilapia grading culture device, during grading culture screening treatment, tilapia with different sizes easily swim and mix with one another, rapid classification screening treatment is inconvenient, and meanwhile, sufficient disinfection treatment on fish fries is inconvenient.

The invention discloses an anti-tilapia mossambica IgM monoclonal antibody, a hybridoma cell strain and application thereof. The CDR sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1-3, and the CDR sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.4-6; the monoclonal antibody can be secreted by a hybridoma cell strain with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2023153. The monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of tilapia mossambica secretion form IgM, can be used for flow cytometry identification of tilapia mossambica IgM + B cells, has the characteristics of good specificity, high sensitivity, strong affinity and the like, can be used for structural analysis, immune response level detection and the like of tilapia mossambica IgM, and can be used for preparing a tilapia mossambica IgM immunoassay kit. And a foundation is laid for deep research of a tilapia immune system and establishment of a tilapia vaccine immune effect evaluation method taking the antibody level as an index.