Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans

A technology of Bacillus coagulans and compound microecology, applied in the direction of microorganisms, microorganisms, animal feed, etc., can solve the problems of heavy losses, nitrite poisoning, and unreported application of farmers, so as to improve growth performance and reduce the number of bait. , the effect of improving the survival rate

Active Publication Date: 2018-05-29
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nitrite poisoning has always been another difficult problem encountered in the breeding process, which often brings heavy losses to farmers
Most of the biodegradation of nitrite requires the participation of oxygen, and the current high-density farming results in insufficient

Method used

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  • Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans
  • Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans
  • Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of Bacillus coagulans CGMCC No.12196

[0035]1. Isolation of bacterial strains: Weigh 1 g of soil sample, place it in 100 ml of sterilized modified MRS (MRS medium with 10 g / L of α-methyl glucoside and 1 g / L of potassium sorbate) liquid medium, and shake for 10 min. Place in a 42°C incubator for enrichment culture for 4 days, shaking once a day. After the enrichment, take 2mL of the liquid and put it in a water bath at 90°C for 10 minutes, and then perform a 10-fold serial dilution. Select three dilutions of 10 times, 100 times and 1000 times, pipette 100 μl into the MRS plate, each dilution is repeated three times, spread evenly with a sterile applicator, and incubate at 42°C for 24 hours. Pick a single colony for Gram staining and microscopic examination, and isolate a Gram-positive, rod-shaped, spore-forming bacterium, streak and purify it three times on the plate, and take a single colony for preservation. The bacterium appea...

Embodiment 2

[0039] Example 2 Hemolytic test of Bacillus coagulans CGMCC No.12196

[0040] The isolated Bacillus coagulans CGMCC No. 12196 was streak-inoculated onto fresh blood culture medium and cultured at 37°C for 24 hours. After observation, no hemolytic ring was found. It shows that the strain has no hemolytic activity and is a relatively safe strain.

Embodiment 3

[0041] Example 3 Bacteriostatic ability test of Bacillus coagulans CGMCC No.12196

[0042] Melt 100 mL of nutrient broth agar (containing 1% glucose) in the Erlenmeyer flask and cool to 45°C. Add 100ul of Streptococcus iniae and Streptococcus agalactiae cultivated overnight, mix well, pour into sterile plate, pour 15-20ml into each plate. Two Oxford cups are pre-placed on each plate, and the Oxford cups are taken out after the agar solidifies, and agar holes are formed on the plate. Add 150 μL of the bacteria solution to be tested to the agar well, set up 3 repetitions, cover the dish cover, carefully move it to a 37°C incubator, put the plate upright, and culture it statically. At the same time, it was compared with two commercially available Bacillus coagulans powders. After culturing for 20 hours, open the lid of the dish and measure the diameter of the inhibition zone with a caliper. Through experiments, it was found that Bacillus coagulans CGMCC No.12196 had a signific...

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Abstract

The invention discloses a bacillus coagulans strain capable of resisting streptococcus and applications of the bacillus coagulans strain. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.12196. The strain is prepared into fungus powder which is added to an animal feed, the fungus powder also can beapplied as a microbial preparation for resisting streptococcus, and after the feed containing the strain is used for feeding tilapia mossambica, streptococcicosis can be effectively prevented and treated. The strain is mixed with bacillus subtilis and lactobacillus johnsonii, then a compound microbial preparation is prepared, and the compound microbial preparation can improve the growth performance of tilapia mossambica, and improve the activity of superoxide dismutase and catalase of the liver and lysozyme in serum. In addition, the fermentation broth of the strain can be adopted as a breeding environment modifier, after the fermentation liquor of the strain is sprayed to an aquaculture pond of tilapia mossambica, the quantity of streptococcus in the aquaculture water can be reduced, andnitrite in the water can be eliminated under the low oxygen condition.

Description

technical field [0001] The invention belongs to the field of feed microecology, and in particular relates to a new strain of bacillus coagulans, a microecological preparation and feed. Background technique [0002] China is the largest tilapia producer in the world, ranking first in the world in output, export volume and export value. Tilapia is the most internationally competitive freshwater aquaculture species in China, and it is also the species with the most industrialized development conditions. It has become a pillar industry in southern China and has strong development advantages in the country. However, the diseases of tilapia caused by Streptococcus agalactiae and Streptococcus iniae are easy to break out in summer and autumn. 25% to 80%, or even higher, has caused great economic losses to the tilapia aquaculture industry, and there is a growing trend. [0003] At present, in tilapia farming and production, people mainly use antibiotic chemical drugs to prevent an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C02F3/34C12R1/07C12R1/125C12R1/225
CPCC02F3/34C12N1/20C12N1/205C12R2001/07
Inventor 汪攀易敢峰孙自博朱传忠付维来刘婷韩科厅陈丽仙
Owner BEIJING DABEINONG TECH GRP CO LTD
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