Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans
A technology of Bacillus coagulans and compound microecology, applied in the direction of microorganisms, microorganisms, animal feed, etc., can solve the problems of heavy losses, nitrite poisoning, and unreported application of farmers, so as to improve growth performance and reduce the number of bait. , the effect of improving the survival rate
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Embodiment 1
[0034] Example 1 Isolation and identification of Bacillus coagulans CGMCC No.12196
[0035]1. Isolation of bacterial strains: Weigh 1 g of soil sample, place it in 100 ml of sterilized modified MRS (MRS medium with 10 g / L of α-methyl glucoside and 1 g / L of potassium sorbate) liquid medium, and shake for 10 min. Place in a 42°C incubator for enrichment culture for 4 days, shaking once a day. After the enrichment, take 2mL of the liquid and put it in a water bath at 90°C for 10 minutes, and then perform a 10-fold serial dilution. Select three dilutions of 10 times, 100 times and 1000 times, pipette 100 μl into the MRS plate, each dilution is repeated three times, spread evenly with a sterile applicator, and incubate at 42°C for 24 hours. Pick a single colony for Gram staining and microscopic examination, and isolate a Gram-positive, rod-shaped, spore-forming bacterium, streak and purify it three times on the plate, and take a single colony for preservation. The bacterium appea...
Embodiment 2
[0039] Example 2 Hemolytic test of Bacillus coagulans CGMCC No.12196
[0040] The isolated Bacillus coagulans CGMCC No. 12196 was streak-inoculated onto fresh blood culture medium and cultured at 37°C for 24 hours. After observation, no hemolytic ring was found. It shows that the strain has no hemolytic activity and is a relatively safe strain.
Embodiment 3
[0041] Example 3 Bacteriostatic ability test of Bacillus coagulans CGMCC No.12196
[0042] Melt 100 mL of nutrient broth agar (containing 1% glucose) in the Erlenmeyer flask and cool to 45°C. Add 100ul of Streptococcus iniae and Streptococcus agalactiae cultivated overnight, mix well, pour into sterile plate, pour 15-20ml into each plate. Two Oxford cups are pre-placed on each plate, and the Oxford cups are taken out after the agar solidifies, and agar holes are formed on the plate. Add 150 μL of the bacteria solution to be tested to the agar well, set up 3 repetitions, cover the dish cover, carefully move it to a 37°C incubator, put the plate upright, and culture it statically. At the same time, it was compared with two commercially available Bacillus coagulans powders. After culturing for 20 hours, open the lid of the dish and measure the diameter of the inhibition zone with a caliper. Through experiments, it was found that Bacillus coagulans CGMCC No.12196 had a signific...
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