43results about How to "No hemolytic activity" patented technology

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

The invention provides a wood frog antibiotic peptide and a preparation process thereof. The invention further discloses application of the antibiotic peptide in the preparation of antiviral medicines. The peptide is a basic polypeptide with the molecular weight range from 2 to 16 KDa and the isoelectric point from 8.9 to 9.5. The peptide consists of asparagic acid, glutamic acid, major serine, histidine, arginine, glycine, threonine, proline, alanine, Val, methionine, and the like, wherein the basic amino acid accounts for 23.99 percent of the gross amount, and the acidic amino acid accounts for 19.08 percent of the gross amount. The wood frog antiviral antibiotic peptide is a product obtained from natural resources. The wood frog antiviral antibiotic peptide has the good antiviral capability and simultaneously has the advantages of no hemolytic activity, no plasma coagulation activity, and the like. The wood frog antibiotic peptide can be used for preparing medicines for treating antiviral type flu, hepatitis B and AIDS.

The invention provides novel synthesis antibacterial peptides and application thereof. The novel synthesis antibacterial peptides are designed and synthesized on the basis of analyzing sequences and structures of natural antibacterial peptides, and the sequences of the novel synthesis antibacterial peptides comprise WYQ-a: ArgArgTrpTrpArg and WYQ-b: PheArgTrpTrpArg. The synthesis antibacterial peptides have the broad spectrum killing activity on gram-positive bacteria and gram-negative bacteria, have higher bactericidal activity than that of the natural antibacterial peptides, and do not have a toxic effect on animal and plant cells. The synthesis antibacterial peptides have broad antibacterial spectra and simple structures, are not needed to be modified and connected and are convenient to synthesize artificially. Experiments prove that: for G+bacteria (the gram-positive bacteria) and G-bacteria (the gram-negative bacteria), the novel synthesis antibacterial peptides have the obvious bacteriostatic and bactericidal activity and do not have hemolysis activity, so the synthesis antibacterial peptides have significant value in the development and application of antibacterial medicines.

The invention relates to a vespa antimicrobial peptide and its preparation method and application. The antimicrobial peptide is a single-chain polypeptide with a molecular weight of 1387.7 Daltons and an isoelectric point of 10. The sequence of the single-chain polypeptide is: isoleucine-tryptophan-tryptophan-lysine-glycine - Isoleucine - Alanine - Alanine - Methionine - Alanine - Lysine - Isoleucine. The preparation method is that the bee venom collected by electrically stimulating wasps is separated and purified through gel filtration column chromatography, ion exchange column chromatography and reversed-phase high-pressure liquid chromatography in sequence. The vespa antimicrobial peptide can be used to prepare medicines for treating diseases infected by pathogenic microorganisms and medicines for treating tumors.

The invention relates to an anti-oxidization small peptide capable of inhibiting melanogenesis as well as a preparation method and application thereof. The anti-oxidization small peptide comprises a short peptide Ansin-2 and a short peptide Magin-2, and belongs to the technical field of biomedicines. The two short peptides have strong oxidization resistance and can inhibit the activity of tyrosinase and obviously reduce melanogenesis; meanwhile, the small peptide further has the characteristics of small molecular weight, simple structure, no cytotoxicity, no hemolytic activity and high tissue penetration, and the preparation method is simple. The anti-oxidization small peptide can be used for preparing a novel beauty-maintaining and skin-care raw material capable of resisting oxidization, resisting melanogenesis and removing spots, also can be used for preparing an anti-oxidization medicine, a food additive and the like, and has an extremely good application prospect.

The present invention relates to rana grahami antibiotic peptide and its application, and belongs to the field of biomedicine. Rana grahami antibiotic peptide is one kind of cyclic polypeptide separated from the secretion of amphibian rana grahami, and has molecular weight of 1705.3 dalton, isoelectric point of 9.39, and primary structure of polypeptide sequence Leu Lys Gly Cys Trp The Lys Ser Ile Pro Pro Lys Pro Cys Phe(LKGCWTKSIPPKPCF) with cysteines in the 4-th and 14-th forming intramolecular disulfide bond. Its preparation process includes collecting secretion of ether stimulated rana grahami, centrifuging to eliminate precipitate, freeze drying, chromatographic separation in filtering gel column and separating purification in reverse high pressure liquid chromatogram. The rana grahami antibiotic peptide has powerful effect of inhibiting bacteria, fungi and viruses and no hemolytic activity, and may be used in preparing medicine for treating pathogenic microbe infection diseases.

The invention discloses a citrus fruit fly antibacterial peptide, which is a polypeptide obtained from separation of a citrus fruit fly pupa, with a molecular weight of 2.3 daltons and an isoelectric point of 11.43, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 1. The invention simultaneously discloses a method for preparing the citrus fruit fly antibacterial peptide, comprising the following steps that: the citrus fruit fly pupa is induced by adoption of a mixed bacteria solution; a crude extract is obtained through tissue homogenate; and the citrus fruit fly antibacterial peptide is obtained after purification through ion exchange chromatography, reversed fast protein liquid phase chromatography and reversed high pressure liquid phase chromatography of the crude extract. The citrus fruit fly antibacterial peptide provided by the invention has inhibition on gram positive bacteria, gram negative bacteria and fungi, has no hemolytic activity, can be taken as a raw material for developing human and poultry biotechnical products with pathogenic microorganism infection resistance, and can be taken as a disease-resistant gene to be used for breeding transgenic plants and transgenic animals by means of gene transformation and other biotechnologies.

The invention relates to a group of antioxidative small peptides for inhibiting melanin production and a preparation method and application thereof. The small peptides comprise a short peptide OGOT, a short peptide Magin-FL and a short peptide Ansin-FL and belong to the technical field of biomedicine. The three short peptides have strong anti-oxidative activity, can inhibit the activity of tyrosinase, can significantly reduce the production of melanin and have the characteristics of low molecular weight, simple structure, no cytotoxicity, no hemolysis activity, high tissue permeability and simple preparation method. The small peptides can be used to prepare novel skin care raw materials with effects of resisting oxidization and melanin production and fading spots, can be used for preparation of anti-oxidant drugs and food additives and have a good application prospect.

The present invention relates to rana grahami antibiotic peptide and its application, and belongs to the field of biomedicine. Rana grahami antibiotic peptide is one kind of cyclic polypeptide separated from the secretion of amphibian rana grahami, and has molecular weight of 1705.3 dalton, isoelectric point of 9.39, and primary structure of polypeptide sequence Leu Lys Gly Cys Trp The Lys Ser Ile Pro Pro Lys Pro Cys Phe(LKGCWTKSIPPKPCF) with cysteines in the 4-th and 14-th forming intramolecular disulfide bond. Its preparation process includes collecting secretion of ether stimulated rana grahami, centrifuging to eliminate precipitate, freeze drying, chromatographic separation in filtering gel column and separating purification in reverse high pressure liquid chromatogram. The rana grahami antibiotic peptide has powerful effect of inhibiting bacteria, fungi and viruses and no hemolytic activity, and may be used in preparing medicine for treating pathogenic microbe infection diseases.

The invention provides collagen secretion promotion ultrashort peptides and application thereof, and belongs to the technical field of biomedicine. The two kinds of collagen secretion promotion ultrashort peptides Magin-FL and Purin-WH3 have the capability of promoting the collagen synthesis; in addition, the characteristics of ultra-high oxidization resistance, aging-resistant activity, no cell toxicity, no hemolysis activity, simple preparation method and the like are realized, so that the collagen secretion promotion ultrashort peptides are used for preparing medicine for treating injured skin restoration and regeneration such as medicine for treating burnt injury, scald injury and skin ulceration, and can replace EGF to be used in the fields of anti-aging and anti-oxidization beatification skin care product new raw materials for skin restoration and skin collagen proliferation simulation and the like. In addition, the effects are possibly not limited to the field. The ultrashort peptides Magin-FL and Purin-WH3 have small molecular weight and simple structure; the used chemical synthesis method has very low cost; the ultrashort peptides Magin-FL and Purin-WH3 have the obvious beneficial characteristics through being compared with EFG with the sale price being nearly 2 million U.S dollars per gram.

The invention relates to bullfrog antibiotic peptide temporin-Lb, genes thereof and the application in the pharmacy, belonging to the biomedicine field. The bullfrog antibiotic peptide genes are obtained by filtering in a constructed bullfrog skin c DNA library; wherein, the molecular weight is 1691.11 dalton; the isoelectric point is 9.70; the primary structure of the complete sequence of the polypeptide is Leu Phe Arg His Val Val Lys Ile PheGIu Lys Tyr Leu-AMIDATION. The genes of the coding bullfrog antibiotic peptide are composed of 322 position nucleotide acid; wherein, the coding mature peptide is 142nd-180th position nucleotide acid. The synthetic bullfrog antibiotic peptide has obvious function of inhibiting the growth of the bacterial and fungus and can be taken as therapeutic drug for preparing pathogenic microorganism infection diseases to be applied.

The invention relates to a preparation method of an application of an artificial antimicrobial peptide MA-D4. According to the invention, an amino acid sequence of the artificial antimicrobial peptide MA-D4 is designed artificially, and a nucleotide sequence encoding the antimicrobial peptide is designed by using an escherichia coli preference codon; a target gene is synthesized by an overlap region amplification gene splicing method, and a prokaryotic expression vector is constructed and is transformed into the escherichia coli BL21 or Rosetta for inducing expression; an expressed product is subjected to digestion treatment by enterokinase and is subjected to nickel ion affinity chromatography purification, so as to obtain the artificial antimicrobial peptide MA-D4. The antimicrobial peptide has higher broad-spectrum antibacterial activity, can inhibit gram-positive bacteria and gram-negative bacteria, and meanwhile, excludes hemolytic activity. Therefore, as a novel antimicrobial drug, the antimicrobial peptide has excellent application prospect on the aspects of food preservation, disease treatment and the like, and has very high developing potential. The preparation method is high in expression efficiency, easy in separation and purification, low in production cost and good in stability, therefore, the preparation method can be used for mass production.

New use of a cryptoid crab antimicrobial peptide Scyreprocin. The antibacterial protein is derived from a cryptoid crab, and is expressed and purified by using a genetic engineered technology. It is first disclosed that the antimicrobial peptide Scyreprocin not only has good antimicrobial activity, but also has a membrane-penetrating function, has an inhibitory and killing activity against various cancer cells, and at the same time has no cytotoxicity against human non-cancer cells. It has demonstrated that the antibacterial polypeptide has a high efficiency and specific anti-cancer activity, shows great application value, and has a good application prospect in research, development and application as an anti-tumor pharmaceutical ingredient. Provided is new use of the cryptoid crab antibacterial peptide Scyreprocin, which broadens the application range of Scyreprocin, and provides more possibilities for the application of Scyreprocin.

The invention provides a collagen secretion promotion ultrashort peptide and application thereof, and belongs to the technical field of biomedicine. The ultrashort peptide comprises at least one kind of materials from ultrashort peptides Magin-WH, Tinin-WH and Orin-G-OT, has the capability of promoting the collagen synthesis, and has the characteristics of very strong oxidization resistance, anti-aging performance, small molecular weight, simple structure, very low cell toxicity, no hemolytic activity, simple preparation method and the like. The ultrashort peptideis used for preparing medicine for treating injured skin restoration and regeneration, such as medicine for treating burnt injury, scald injury and skin ulceration, and can replace EGF to be used in the fields of anti-aging and anti-oxidization beatification skin care product new raw materials for skin restoration and skin collagen proliferation simulation and the like. The ultrashort peptide has small molecular weight and simple structure; the used chemical synthesis method has very low cost; the ultrashort peptide has the obvious beneficial characteristics through being compared with EFG with the sale price being nearly 2 million U.S dollars per gram.

The invention provides a cordycep militaris ethyl acetate extract and preparing process thereof, further discloses a purpose of the extract for preparing antiviral remedies. The preparing process comprises: smashing the dry cordyceps militaris, adding ethyl acetate, extracting and filtering in back flow manner by a Soxhlet extractor, and getting a filtrate; eluting the residue by ion liquid, filtering and get another filtrate; combining the tow filtrates, decompressing and concentrating to a constant weight to obtain the final extract. Extracting the effective constituents of the cordycep militaris by using ethyl acetate can increase the extraction rates of multiple effective constituents to higher limit, thereby improve medicine effect. The cordycep militaris ethyl acetate extract is a product obtained from natural resources, achieves excellent antivirus capacity, and advantages of no hemolytic activity and no plasma clkotting activity verified by experiment, can be used for preparing medicine for preventing AIDS, hepatitis B and virus form flu. The invention provides a new selection to the medicine for preventing AIDS, hepatitis B and virus form flu utilizing the abundant natural resources in our country.

The invention discloses a novel broad-spectrum antibacterial peptide SAMP1-A3 and a preparation method thereof. The amino acid sequence of the antibacterial peptide is NH2-RKKKVLLI-COOH. The preparation method of the invention adopts a solid-phase polypeptide synthesis means protected by amino acid nitrogen Fmoc. The method overcomes the defects of poor biological safety, easy hemolysis or toxicity to mammalian cells of common antibacterial peptides, and the novel antibacterial peptide has broad-spectrum inhibition effect on non-filamentous fungi and bacteria, does not have hemolysis and has no toxicity to normal mammalian cells. The polypeptide provides a novel leader for clinical antifungal drug research.

The invention discloses a bactrocerin, and a preparation method and an application thereof. The bactrocerin is a polypeptide separated from pupas of Bactrocera dorsalis, the molecular weight is 2.3 Dalton, the isoelectric point is 11.43, and the amino acid sequence of the bactrocerin is represented by SEQ ID NO:1. The invention also discloses the preparation method of the antimicrobial peptides of the bactrocerin. The method comprises the following steps: inducing the pupas of Bactrocera dorsalis with a mixed bacterial liquid, carrying out tissue homogenization to obtain a crude extract, and carrying out ion exchange chromatography, reversed phase fast protein liquid chromatography and reversed phase high pressure liquid chromatography purification on the crude extract to prepare the bactrocerin. The bactrocerin has an inhibition effect on Gram-positive bacteria, Gram-negative bacteria and fungi, has no hemolytic activity, can be used as a raw material for developing biotechnological products for preventing pathogenic organism infection of humans and animals, and also can be used in transgenic plants and transgenic animal breeding as an anti-disease gene through transgene and other biotechnologies.

The invention relates to a group of antioxidative small peptides for inhibiting melanin production and a preparation method and application thereof. The small peptides comprise a short peptide OGOT, a short peptide Magin-FL and a short peptide Ansin-FL and belong to the technical field of biomedicine. The three short peptides have strong anti-oxidative activity, can inhibit the activity of tyrosinase, can significantly reduce the production of melanin and have the characteristics of low molecular weight, simple structure, no cytotoxicity, no hemolysis activity, high tissue permeability and simple preparation method. The small peptides can be used to prepare novel skin care raw materials with effects of resisting oxidization and melanin production and fading spots, can be used for preparation of anti-oxidant drugs and food additives and have a good application prospect.

The invention relates to an anti-oxidization small peptide capable of inhibiting melanogenesis as well as a preparation method and application thereof. The anti-oxidization small peptide comprises a short peptide Ansin-2 and a short peptide Magin-2, and belongs to the technical field of biomedicines. The two short peptides have strong oxidization resistance and can inhibit the activity of tyrosinase and obviously reduce melanogenesis; meanwhile, the small peptide further has the characteristics of small molecular weight, simple structure, no cytotoxicity, no hemolytic activity and high tissue penetration, and the preparation method is simple. The anti-oxidization small peptide can be used for preparing a novel beauty-maintaining and skin-care raw material capable of resisting oxidization, resisting melanogenesis and removing spots, also can be used for preparing an anti-oxidization medicine, a food additive and the like, and has an extremely good application prospect.

The invention discloses a strain of Bacillus coagulans antagonizing streptococcus and its application. The bacterial strain has been preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC No.12196. The bacterial strain is made into bacteria powder, which can be added to animal feed, and can also be used as a microbial preparation for antagonizing streptococci. Feeding tilapia with the feed containing the bacterial strain can effectively prevent and treat streptococcal disease. Mix the bacteria with Bacillus subtilis and Lactobacillus johnsonii to make a compound microecological preparation, which can improve the growth performance of tilapia, increase the activity of liver superoxide dismutase, catalase and lysozyme in serum . In addition, the fermented liquid of the bacteria can also be used as a culture environment improver. Sprinkling the fermented liquid of the above bacteria into the tilapia culture pond can reduce the number of streptococci in the culture water, and can also remove the streptococci in the water under low oxygen conditions. nitrite.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

Disclosed is an alkaline antibacterial peptide consisting of leucine and lysine. The alkaline antibacterial peptide comprises 12-24 amino acids, wherein the proportion of lysine is more than 33.3%. Also disclosed is a targeted antibacterial peptide formed by the connection of the alkaline antibacterial peptide and the complementarity determining region of an antibody through leucine. The alkaline antibacterial peptide and the targeted antibacterial peptide can be used as an antibacterial and for cancer resistance.

The invention discloses antibacterial peptide of bactrocera dorsalis hendel and derivatives and application of the antibacterial peptide. An amino acid sequence of antibacterial peptide is as shown in SEQ ID NO:1; the derivatives of antibacterial peptide are antibacterial active derivatives obtained through modification or antibacterial derivatives formed through substitution, deletion or addition of one or more amino acid residues; obtained antibacterial peptide BH-AMP003 has bioactivity and a wide antibacterial effect, so that antibacterial peptide BH-AMP003 can be applied to preparation of antibacterial products including feed additives, animal drugs, preservatives and medical products such as antibacterial drugs.

The invention provides an antifungal antibacterial peptide GL4W and its preparation method and application. The sequence of the antibacterial peptide GL4W is shown in SEQ ID No.1. Taking the antimicrobial peptide L4 (RLRLLRLR) as the active center, a cap structure was formed with glycine at its N-terminus, and tryptophan was added at its C-terminus to form a tail anchor structure to design an antimicrobial peptide, which was named GL4W. Application of the antimicrobial peptide GL4W in the preparation of drugs for treating infectious diseases caused by fungi. The invention changes the antibacterial spectrum of antibacterial peptide L4, becomes a kind of antifungal antibacterial peptide, and improves the selectivity of antibacterial peptide to bacterial cells, which has the development potential of antifungal infection drug, and the therapeutic index of antibacterial peptide GL4W is as high as 39.61.

The invention discloses antibacterial peptide of bactrocera dorsalis hendel and derivatives and application of the antibacterial peptide. An amino acid sequence of antibacterial peptide is as shown in SEQ ID NO:1; the derivatives of antibacterial peptide are antibacterial active derivatives obtained through modification or antibacterial derivatives formed through substitution, deletion or addition of one or more amino acid residues; obtained antibacterial peptide BH-AMP003 has bioactivity and a wide antibacterial effect, so that antibacterial peptide BH-AMP003 can be applied to preparation of antibacterial products including feed additives, animal drugs, preservatives and medical products such as antibacterial drugs.

The invention discloses a degradable antibacterial peptide based on cellulose and a preparation method thereof. Comprising the following steps: step 1, adding cellulose into a sulfuric acid solution, carrying out acid treatment, and terminating the reaction with ethanol; centrifuging, washing and freeze-drying to obtain cellulose A; 2, dispersing cellulose A in deionized water to obtain a cellulose solution; the preparation method comprises the following steps: adjusting the pH value to 9, adding 1-(3-dimethylaminopropyl)-3-ethyl carbene imide and N-hydroxyl thiosuccinimide sodium salt, and stirring for 2 hours; adding excessive diamino compound, stirring for 10-12 hours, adding methoxy polyethylene glycol amine, and reacting for a period of time; carrying out ethanol precipitation, centrifugation, washing and freeze drying; the preparation method comprises the following steps: ultrasonically dispersing in water, adjusting the pH value to 3.5 by using 0.1 M hydrochloric acid, stirring for 2-4 hours, washing, dialyzing and freeze-drying to obtain the degradable antibacterial peptide.

The present invention belongs to the field of biomedicine technology, and is especially Amolops loloensis insulin release promoting peptide and its application in preparing medicine. The present invention obtains insulin release promoting peptide from the skin secretion of Amolops loloensis through a conventional biochemical process, measures its sequence and synthesizes the polypeptide based on the obtained sequence. Amolops loloensis insulin release promoting peptide is one kind of single strand polypeptide of molecular weight of 1627.04 dalton, isoelectri point of 8.75 and total sequence of Phe-Leu-Pro-Ile-Val-Gly-Lys-Leu-Leu-Ser-Gly-Leu-Ser-Gly-Leu- Leu(FLPIVGKLLSGLSGLL-NH2). It has excellent insulin release promoting effect, no hemolytic activity, no plasma clotting activity and other advantages, and may be used in preparing medicine for treating diabetes.

A broad-spectrum high-efficiency antimicrobial peptide Pb-CATH-OH1 and its gene, preparation method and application, which are straight-chain polypeptides, sequence: lysine-arginine-phenylalanine-lysine-lysine- Phenylalanine‑Phenylalanine‑Arginine‑Lysine‑Isoleucine‑Lysine‑Lysine‑Glycine‑Phenylalanine‑Arginine‑Lysine‑Isoleucine ‑Phenylalanine‑Lysine‑Lysine‑Threonine‑Lysine‑Isoleucine‑Phenylalanine‑Isoleucine‑Glycine‑Glycine‑Threonine‑Isoleucine‑ Proline‑Isoleucine. The precursor gene consists of 528 nucleotides, nucleotides 433‑525. It is rich in basic amino acids, small in molecular weight, simple in structure, has extremely strong broad-spectrum antimicrobial activity, is active against Gram-positive bacteria, Gram-negative bacteria and fungi, has low hemolysis, and is not easy to produce drug resistance. It is prepared as an additive Drugs, cosmetics, food and feed for anti-pathogenic microbial infection.