108 results about "Grass Carp (fish)" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention provides a grass carp sourced U6 promoter. The sequence of the grass carp sourced U6 promoter is shown as one of SEQ ID NO: 1-3. Three grass carp-derived U6 promoters are cloned, the transcriptional activity and cross-species adaptability of the three promoters are analyzed, results show that the three promoters can show transcriptional activity in fish cells and human cells, the transcriptional activity of ciU6-1 is remarkably higher than that of the other two promoters, heterologous DNA can be transcribed in various cells, and the expression of the ciU6-1 in the fish cells and the human cells is improved. And the method can be used for gene editing means based on Cas9.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The invention relates to the technical field of fish genetic breeding, in particular to a method for improving grass carp reovirus resistance of grass carp. Four specific knockout targets of the herc4 gene are disclosed, the grass carp herc4 gene is specifically knocked out by utilizing a CRISPR / Cas9 gene editing technology, and a herc4 gene knockout individual, namely the high-resistance grass carp reovirus fish, is obtained. According to the invention, the function of the herc4 in the grass carp is verified for the first time, a new grass carp germplasm with improved grass carp reovirus resistance is rapidly obtained by knocking out the single gene of the herc4, and the herc4 has important industrial value.

The invention relates to the technical field of aquaculture fish vaccines, in particular to a fusion gene of grass carp NPM1a and II type reovirus VP38, recombinant protein, a composition and application. The nucleotide sequence of the fusion gene is as shown in SEQ ID NO: 1. The fusion gene is obtained by connecting grass carp NPM1a and II type reovirus VP38 through a linker sequence, a BamH restriction enzyme cutting site is added to the 5'end of the fusion sequence, and an XhoI restriction enzyme cutting site is added to the 3 'end of the fusion sequence; the sequence of the linker is as shown in SEQ ID NO: 2. The subunit vaccine of the fusion protein of the grass carp NPM1a and the II type reovirus VP38 is prepared, and indole-3-lactic acid is used as an immunopotentiator in a combined manner, so that the technical bottlenecks of low protection rate, poor intestinal mucosal barrier repairability and the like of the existing vaccine are overcome.

The invention discloses sgRNAs of a specific targeting grass carp RIOK3 gene and a construction method and application of an RIOK3 gene knockout cell line, and belongs to the technical field of gene engineering. The invention provides sgRNAs of a specific targeting grass carp RIOK3 gene and a sequence of a primer group, and also provides a method for constructing a large-fragment knockout cell line of the RIOK3 gene in combination with the sgRNAs, and application of the gene knockout cell line in research on an anti-virus infection mechanism of grass carp. The invention discloses the construction of the grass carp gene in-vitro research model by knocking out the antiviral negative regulatory factor RIOK3 of the fish cells by using the CRISPR-Cas9 technology for the first time, provides an in-vitro research material and a new way for the analysis of an antiviral immune regulation molecular mechanism of the grass carp, and particularly has important guiding significance for the research of resisting grass carp reovirus infection.

The invention relates to the technical field of sewage purification, and particularly discloses a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The invention relates to a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The method comprises the following steps: S1, collecting, adjusting and precipitating sewage through a pretreatment unit, and intercepting solid garbage at the same time; s2, introducing the pretreated sewage into a fish-fungus-algae symbiotic unit, putting fungi and algae into the unit, and stocking herbivorous fishes; s3, effluent of the fish-fungus-algae symbiosis unit is guided into a shellfish purification unit, and shellfish is put into the shellfish purification unit; s4, leading effluent of the shellfish purification unit into an aquatic plant purification unit, and planting aquatic plants in the aquatic plant purification unit. The pollution reduction type water quality purification method combining the shellfish and grass carp bacteria and algae can be used for scenes such as village and town domestic sewage, agricultural non-point source pollution and sewage treatment plant tail water, and has the advantages of pollutant reduction and water body self-purification capability reconstruction.

The invention provides an application of an NFKBIA gene in identification of salt tolerance of fish. Through experiments, the inventor finds that the expression quantity of the NFKBIA gene in the grass carp group subjected to salinity stress is obviously higher than that of the grass carp group without salinity stress, and the relative expression quantity of the NFKBIA gene in the freshwater grass carp group and the relative expression quantity of the NFKBIA gene in the salinity training grass carp group are obviously different; the invention reveals that the NFKBIA gene can obviously influence the tolerance of the grass carp to the salt stress, and the NFKBIA gene has a negative regulation effect on the salt stress coping process of the grass carp, and the expression quantity of the NFKBIA gene is closely related to the salt tolerance of the grass carp. The invention discovers that the expression quantity of the NFKBIA gene has significant correlation with the salt tolerance of grass carp for the first time, provides a new target for the research of fish salt tolerance molecular mechanism, enriches the understanding of fish salt stress adaptation mechanism, and provides molecular biology basis for the breeding of fish salt tolerance varieties and the optimization of culture environment.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

A kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample, the present application relates to the field of microplastic toxicity exposure risk evaluation, specifically relates to a kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample.The present application is to solve the problem that the existing evaluation method is single, cannot comprehensively and accurately evaluate the toxicity exposure risk of microplastic to fish.Method: I, set experimental group;II, input wild grass carp to experimental group, record body length and weight;III, feed cultivation, respectively add corresponding microplastic particles, the control group does not add microplastic particles;IV, combine the weight change of wild grass carp, body length change, feeding rate, mortality and intestinal microbial diversity index for calculation;The higher the value of comprehensive index R, the higher the toxicity risk of microplastic to fish is evaluated.The present application is used to evaluate the toxicity exposure risk of microplastic to fish with wild grass carp as sample.

The application belongs to the technical field of fish variety improvement, and discloses a method for creating gynogenetic grass carp with rapid growth. The method comprises the following steps: screening sexually mature male mirror carp and sexually mature female grass carp with obvious growth advantages and stress resistance, collecting sperm of the mirror carp, diluting the sperm with Hank's solution, inactivating the sperm by ultraviolet irradiation, mixing the obtained inactivated sperm with middle-stage eggs of the grass carp, and then performing cold shock doubling treatment, and adding normal temperature water in batches for hatching to obtain gynogenetic grass carp. The gynogenetic grass carp induced by the mirror carp sperm performs excellently in growth speed and disease resistance, which provides a new method and idea for inheritance improvement of the grass carp, provides more possibilities for screening of excellent varieties, and brings greater economic benefits to the grass carp breeding industry.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

The invention belongs to the technical field of fish genetic breeding in aquaculture industry, and discloses a grass carp intensive high-yield character genome selective breeding method, which comprises the following steps: establishing a grass carp intensive high-yield character prediction model; the establishment of the grass carp intensive high-yield character prediction model comprises the following steps: (1) obtaining a grass carp candidate parent fish single nucleotide polymorphism site; (2) constructing an analysis model for predicting breeding values of candidate parents; and (3) constructing a molecular genetic relationship matrix by using the obtained single nucleotide polymorphic sites, and predicting a genome estimation breeding value of the candidate group in combination with the analysis model in the step (2). The improved variety breeding method based on grass carp genome information can be used for screening excellent parents suitable for intensive breeding, the screened parents are directly used for breeding offspring seeds, and the high-density breeding tolerance of grass carp can be directly improved; and a technical support is provided for improving cultivation of new grass carp varieties suitable for intensive and facility cultivation.

The present application relates to the technical field of aquaculture, and particularly discloses a method and system for culturing Opsariichthys pachycephalus fry in a ring ditch of a rice field, which comprises the following steps: selecting a rice field and digging a ring ditch around the rice field; digging a fish ditch in the rice field and digging to the fish ditch being connected with the ring ditch; selecting a first-stage culture area in the ring ditch as a culture area for Opsariichthys pachycephalus fry and arranging double-layer blocking nets at two inner walls of the ring ditch; draining and disinfecting the ring ditch; injecting water into the ring ditch; applying amino acids and special organic fertilizer for water quality culture; selecting Opsariichthys pachycephalus fry in a horizontal swimming stage and putting the fry into the water body; feeding the first-stage culture area with the first feed; starting to feed the second feed; injecting water into the first-stage culture area again; adding microbial agents; removing the inner blocking net; pulling the net to count the number of fry and removing the outer blocking net. The present application has the characteristics of reducing stress damage of Opsariichthys pachycephalus, lowering mortality, having less loss and lower breeding cost.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.

The invention provides application of Hnf4alpha or a biological material containing Hnf4alpha in preparation of a product for inhibiting bacteria and / or viruses, and belongs to the technical field of biological medicines. In-vivo and in-vitro experiments show that overexpression of the Hnf4alpha not only can effectively prevent infection of aeromonas salmonicida and grass carp hemorrhagic disease viruses, but also can remarkably inhibit co-infection of the aeromonas salmonicida and the grass carp hemorrhagic disease viruses. The bacterial or virus load after infection is obviously reduced, and the activity of cells and the survival rate of juvenile fish are improved. The invention provides a molecular target for prevention and control of bacterial infectious diseases, virus infectious diseases and mixed infectious diseases of cyprinid fishes.

The invention discloses application of rhizoma osmundae in prevention and treatment of ichthyophthiriasis of aquaculture animals, toxicity experiments of rhizoma osmundae on grass carp kidney cells (CIK) show that the maximum non-toxic concentration of an aqueous extract of rhizoma osmundae on the CIK cells is 1200 [mu] g / mL, and the inhibition of the aqueous extract of rhizoma osmundae on the survival rate of a grazing body of ichthyophthiriasis has dose correlation. When the concentration of the medicine reaches 100 mg / L (mu g / mL), the death rate of the grazing body reaches 100%, and in addition, calculation shows that the median lethal concentration (LD50) of the rhizoma osmundae aqueous extract to the ichthyophthirius multifiliis grazing body is 13.256 mg / L. The application of the rhizoma osmundae in inhibiting the survival rate of the ichthyophthirius multifiliis of the fish under the cell non-toxic concentration is provided for the first time, a novel medicine is provided for preventing and treating ichthyophthirius multifiliis infection of aquaculture animals, and the rhizoma osmundae is a natural medicinal plant, is environmentally friendly and has the advantages of reducing the risk of medicine residues and the like.

The present application belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carp, and specifically discloses a method for improving salt tolerance of fish, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, and placing the fish fertilized eggs in salt water for hatching and domestication; thereafter, the salinity of the water body is increased by 1‰-2‰ every 25-35 days until the salinity is the same as that of the target aquaculture water body. The present application provides a footprint method based on salinity gradual domestication to improve the salt tolerance of fish. The method can effectively activate the expression of tissue osmotic pressure regulation related genes such as gills and kidneys, and ultimately can enable grass carp to adapt to 7‰ water body salinity, breaking the conventional salt tolerance threshold of grass carp. The main performance is that the grass carp still has the characteristics of rapid growth, healthy body, and strong feeding under 7‰ water body salinity.

The application provides a SNP molecular marker for identifying salt tolerance of grass carp and application thereof. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO:1, wherein the base at the 503th position of the sequence is T or C. The SNP molecular marker provided by the application can be applied to rapid identification of salt tolerance of grass carp and assisted breeding of salt-tolerant grass carp. By detecting the SNP molecular marker, salt-tolerant grass carp parents with excellent salt tolerance can be effectively selected under the same breeding conditions, the SNP molecular marker can be effectively used for molecular marker assisted breeding of grass carp, and then the genotypes of the grass carp parents can be identified according to actual breeding requirements, suitable genotype grass carp parents are selected for breeding, and grass carp offspring (fish fry) with high salt tolerance can be obtained, breeding time is saved, cost is low, accuracy is high, the breeding process of the grass carp is accelerated, and the economic benefits of the grass carp in a saline-alkali environment are increased.

The invention discloses water circulation multistage filtering equipment for grass carp fry breeding, and relates to the technical field of fish culture, a fixed aeration tank is mounted on one side of the top end of a fixed supporting frame, an interception limiting frame is mounted on one side of the top end of the fixed aeration tank, and an interception limiting barrel is clamped to the top of the side end of the interception limiting frame; the bottom of the side end of the interception limiting barrel is connected with a drainage linkage pipe in a penetrating mode at equal intervals, one end of the interception limiting barrel is connected with an in-out electric push rod in a clamped mode, and one end of the in-out electric push rod is connected with an in-out sealing frame in a clamped mode. The water purification effect and efficiency are improved, the filtering speed is improved through circulating cooperation, the electric push rod is used for direct taking and placing treatment, the taking and placing speed of the equipment is improved, and therefore internal slag removal can be rapidly completed, the maintenance labor intensity of workers is relieved, and the time needed by water filtering is shortened.

The invention provides a self-adaptive automatic fish killing device for small and medium-sized grass carps, and relates to the technical field of fresh food processing machinery. Comprising a rack, and a feeding device, an abdominal scale removing device, a roller device, a back scale removing device, a conveying device and a belly opening and viscera removing device which are sequentially arranged on the rack along the technological process. According to the multi-stage self-adaptive flexible scaling system, a floating knife roll is matched with a high-rigidity torsion spring, so that enveloping and scaling of fish bodies with different curvatures are realized; the special-shaped lawn pattern conveying belt module is adopted, so that the problems of slipping and flexible centering clamping of wet and slippery fish bodies are solved; the servo motor is matched with the lifting module to drive the laparotomy tool, and micron-level cutting depth control is achieved. The device is small in size, full-process automation is achieved, and the problems that existing equipment cannot adapt to thick and hard scales of grass carps, gallbladders are prone to being broken when laparotomy is conducted, and manual processing efficiency is low are effectively solved.

The invention discloses a grass carp reovirus type II mRNA (messenger Ribonucleic Acid) vaccine as well as a preparation method and application thereof, and belongs to the field of vaccines. According to the technical scheme, the grass carp reovirus type II mRNA vaccine comprises a modified mRNA chain, the modified mRNA chain sequence comprises a 5 'UTR sequence, a Kozak sequence, a grass carp immunoglobulin mu heavy chain signal peptide coding sequence, a GCRV II outer capsid protein VP35 coding sequence, three termination codons, a 3' UTR sequence and a polyA nucleotide sequence. The GCRV II mRNA vaccine is applied to enhancing the GCRV infection resistance of a fish body, the death rate is reduced, and the blank of the current grass carp reovirus II mRNA vaccine is filled.

The invention belongs to the technical field of aquaculture, and relates to a new application of capsaicin, in particular to an application of capsaicin in preparation of drugs and / or feed additives for preventing and treating fish virus infection. The core of the invention lies in that the capsaicin has an obvious protection effect on infection of spring viraemia of carp virus (SVCV), grass carp reovirus (GCRV) and herpesvirus type 2 (CyHV-2), and can obviously inhibit transcription of SVCV, GCRV and CyHV-2 virus genes and expression of virus proteins. The capsaicin-containing feed additive and / or medicine can be used for preventing and treating fish virus diseases, and has the advantages of high protection efficiency and environmental friendliness.

This invention relates to an early selection and evaluation method for salt tolerance in grass carp based on serum metabolic markers. The method includes: (1) calculating the salinity sensitivity coefficient based on the serum metabolic markers obtained through screening: SSC = (normal control group index value - salinity treatment group index value) / normal control group index value, the larger the value, the more sensitive the fish; (2) calculating the fuzzy membership index based on the SSC value; (3) calculating the weight coefficient of each serum metabolic marker based on principal component analysis. w =Contribution rate of a single indicator / Cumulative contribution rate of principal components; (4) Calculate the comprehensive evaluation value of grass carp's salinity sensitivity based on fuzzy membership index and serum metabolic marker weight coefficient; (5) Salt tolerance assessment: In the early salt tolerance assessment, the higher the comprehensive evaluation value of salinity sensitivity, the worse the salt tolerance. This method can achieve accurate assessment of grass carp's salinity sensitivity by detecting and analyzing the dynamic changes of specific serum metabolic markers.

The application discloses a feed composition and a preparation method and application thereof, and relates to the technical field of feed additives.The feed composition comprises the following components in parts by weight: 4-6 parts of protease, 1-3 parts of phytase, 5-8 parts of bacillus subtilis, 5-8 parts of bacillus coagulans, 40-60 parts of oil, and 2-4 parts of emulsifier.The specific bacteria and enzymes are used for synergistic effect in the feed composition, so that the prepared feed can not only eliminate the muddy smell of farmed fish, but also reduce the abdominal fat rate of grass carp and improve the tightness of fish meat.

The invention discloses a method for constructing a novel low-fat-deposition-rate fish germplasm based on Ppargamma gene knockout, which comprises the following steps: specifically knocking out a fish Ppargamma gene by using a CRISPR / Cas9 gene editing technology, constructing a fish Ppargamma deletion mutant, screening a novel low-fat grass carp germplasm, reducing the fat deposition rate of a fish body, and culturing a novel low-fat-content fish variety. The problems of accumulation of mesenteric fat of the grass carp, excessive accumulation of body fat of nutritional fatty liver and the like are effectively solved. The grass carp Ppargamma gene is directionally edited through a gene editing technology, support is provided for molecular mechanism research of fish nutrition, the important scientific research value is achieved, the function of the Ppargamma gene in fish fat metabolism can be deeply analyzed, the remarkable industrial application value is achieved, sustainable development of the aquaculture industry can be promoted, and the application prospect is wide. The requirements of the market on low-fat and high-nutritional-value fish products are met. The low-fat grass carp new germplasm cultivated by the method can provide a new technical approach and germplasm resources for fish nutrition improvement and healthy cultivation.

The utility model discloses a flexible cord flap hook, which relates to the field of fishing gear, and comprises a flap component arranged at the bottom of square bait, and a main cord penetrates through the flap component and extends to the bottom of the flap component; the turning plate assembly comprises a turning plate; the plurality of turning plate holes are distributed in a circumferential array shape and are formed in the outer side of the turning plate in a penetrating manner; according to the flexible line flap hook, the sub-lines are arranged to be the long sub-lines and the short sub-lines, the length of the short sub-lines is smaller than the vertical distance from the flap holes connected with the short sub-lines to the long sub-lines, the situation that the adjacent sub-lines are mutually wound when moving in water is reduced, the long sub-lines provide larger-range coverage, meanwhile, a larger fishhook is bound, and the flexible line flap hook is convenient to use. The small fishhook of the short sub-line can not reach the mouth of the big fish after being separated from the square bait aiming at the fish with the big mouth feeding mode of long-distance bait suction, such as silver carp, bighead carp and grass carp, so that the big fish is prevented from escaping when eating the small hook.

The present application relates to long-nosed grass carp breeding technical field, especially green pollution-free long-nossed grass carp breeding method, aiming at the single purification effect of the breeding water in the green breeding process of long-nosed grass carp in the prior art, the present application provides the following scheme, which comprises the following steps: S1, establishing a breeding farm, building suitable breeding ponds according to the breeding scale, and laying bricks and stones or cement hardening treatment on the bottom and side of the breeding pond; S2, establishing a water purification device, installing a certain number of water purification devices in the breeding pond, and regularly starting to purify the breeding water in the breeding pond; S3, prevention and treatment of diseases and insect pests, before putting fish fry, the bottom is comprehensively sprayed with quicklime, the present application not only can conveniently purify the breeding water in the breeding pond, but also can effectively improve the oxygen content in the breeding water in the filtering process, and can also avoid the winding of the water tank on the suction pipe.