136 results about "Grass Carp (fish)" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention provides a grass carp sourced U6 promoter. The sequence of the grass carp sourced U6 promoter is shown as one of SEQ ID NO: 1-3. Three grass carp-derived U6 promoters are cloned, the transcriptional activity and cross-species adaptability of the three promoters are analyzed, results show that the three promoters can show transcriptional activity in fish cells and human cells, the transcriptional activity of ciU6-1 is remarkably higher than that of the other two promoters, heterologous DNA can be transcribed in various cells, and the expression of the ciU6-1 in the fish cells and the human cells is improved. And the method can be used for gene editing means based on Cas9.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The invention relates to the technical field of fish genetic breeding, in particular to a method for improving grass carp reovirus resistance of grass carp. Four specific knockout targets of the herc4 gene are disclosed, the grass carp herc4 gene is specifically knocked out by utilizing a CRISPR / Cas9 gene editing technology, and a herc4 gene knockout individual, namely the high-resistance grass carp reovirus fish, is obtained. According to the invention, the function of the herc4 in the grass carp is verified for the first time, a new grass carp germplasm with improved grass carp reovirus resistance is rapidly obtained by knocking out the single gene of the herc4, and the herc4 has important industrial value.

The invention relates to the technical field of aquaculture fish vaccines, in particular to a fusion gene of grass carp NPM1a and II type reovirus VP38, recombinant protein, a composition and application. The nucleotide sequence of the fusion gene is as shown in SEQ ID NO: 1. The fusion gene is obtained by connecting grass carp NPM1a and II type reovirus VP38 through a linker sequence, a BamH restriction enzyme cutting site is added to the 5'end of the fusion sequence, and an XhoI restriction enzyme cutting site is added to the 3 'end of the fusion sequence; the sequence of the linker is as shown in SEQ ID NO: 2. The subunit vaccine of the fusion protein of the grass carp NPM1a and the II type reovirus VP38 is prepared, and indole-3-lactic acid is used as an immunopotentiator in a combined manner, so that the technical bottlenecks of low protection rate, poor intestinal mucosal barrier repairability and the like of the existing vaccine are overcome.

The invention discloses sgRNAs of a specific targeting grass carp RIOK3 gene and a construction method and application of an RIOK3 gene knockout cell line, and belongs to the technical field of gene engineering. The invention provides sgRNAs of a specific targeting grass carp RIOK3 gene and a sequence of a primer group, and also provides a method for constructing a large-fragment knockout cell line of the RIOK3 gene in combination with the sgRNAs, and application of the gene knockout cell line in research on an anti-virus infection mechanism of grass carp. The invention discloses the construction of the grass carp gene in-vitro research model by knocking out the antiviral negative regulatory factor RIOK3 of the fish cells by using the CRISPR-Cas9 technology for the first time, provides an in-vitro research material and a new way for the analysis of an antiviral immune regulation molecular mechanism of the grass carp, and particularly has important guiding significance for the research of resisting grass carp reovirus infection.

The invention relates to the technical field of sewage purification, and particularly discloses a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The invention relates to a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The method comprises the following steps: S1, collecting, adjusting and precipitating sewage through a pretreatment unit, and intercepting solid garbage at the same time; s2, introducing the pretreated sewage into a fish-fungus-algae symbiotic unit, putting fungi and algae into the unit, and stocking herbivorous fishes; s3, effluent of the fish-fungus-algae symbiosis unit is guided into a shellfish purification unit, and shellfish is put into the shellfish purification unit; s4, leading effluent of the shellfish purification unit into an aquatic plant purification unit, and planting aquatic plants in the aquatic plant purification unit. The pollution reduction type water quality purification method combining the shellfish and grass carp bacteria and algae can be used for scenes such as village and town domestic sewage, agricultural non-point source pollution and sewage treatment plant tail water, and has the advantages of pollutant reduction and water body self-purification capability reconstruction.

The invention discloses a multi-species mutualism-based mussel and fish polyculture ecological regulation and control method, and relates to the technical field of ecological culture, and the method comprises the following steps: S1, facility preparation; s2, putting species; s3, breeding regulation and control; s4, feeding control; and S5, performing final-period management. Through strict screening of the culture pond, thorough disinfection and scientific cultivation of water quality, an excellent starting end is created for culture, the disease risk is reduced, beneficial microbial communities enhance the self-cleaning capacity of a water body, the feeding quantity and proportion are accurately calculated, the ecological capacity is fully utilized, the fingerlings are reasonably matched, stable operation of an ecological system is guaranteed, and the ecological system is more intelligent and reliable through real-time monitoring and intelligent prediction. The feeding amount and the water changing frequency are adjusted in time, the water quality is effectively maintained, the breeding loss caused by water quality deterioration is reduced, grass carp feeding is adjusted according to the aquatic plant coverage rate, reasonable growth of aquatic plants is maintained, ecological balance is promoted, the win-win situation of ecological benefits and economic benefits is achieved, and an effective mode is provided for sustainable aquaculture.

The invention provides an application of an NFKBIA gene in identification of salt tolerance of fish. Through experiments, the inventor finds that the expression quantity of the NFKBIA gene in the grass carp group subjected to salinity stress is obviously higher than that of the grass carp group without salinity stress, and the relative expression quantity of the NFKBIA gene in the freshwater grass carp group and the relative expression quantity of the NFKBIA gene in the salinity training grass carp group are obviously different; the invention reveals that the NFKBIA gene can obviously influence the tolerance of the grass carp to the salt stress, and the NFKBIA gene has a negative regulation effect on the salt stress coping process of the grass carp, and the expression quantity of the NFKBIA gene is closely related to the salt tolerance of the grass carp. The invention discovers that the expression quantity of the NFKBIA gene has significant correlation with the salt tolerance of grass carp for the first time, provides a new target for the research of fish salt tolerance molecular mechanism, enriches the understanding of fish salt stress adaptation mechanism, and provides molecular biology basis for the breeding of fish salt tolerance varieties and the optimization of culture environment.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention belongs to the field of fish immunology, and discloses a rat anti-grass carp CD4-1 monoclonal antibody capable of recognizing CD4-1 + T cells of various cyprinid fishes, a hybridoma cell strain and application. The preservation number of the hybridoma cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024235. Compared with the existing grass carp CD4-1 monoclonal antibody, the monoclonal antibody has the advantages that the cell grouping of flow cytometry staining by using the monoclonal antibody is more obvious, the specificity is higher, and the monoclonal antibody can be used for specifically identifying CD4-1 + T cells of six cyprinid fishes such as grass carp, black carp, silver carp, bighead carp, crucian carp and / or megalobrama amblycephala by using the flow cytometry; an important tool is provided for the subsequent research on the effect and mechanism of the CD4-1 + T cell in the immune response of the cyprinid fish.

The invention discloses an application of a caffeic acid phenethyl ester high-fat feed in improving the overwintering health condition of grass carp and reducing the fat loss rate, and belongs to the technical field of aquaculture. According to the method, the caffeic acid phenethyl ester is added into the high-fat feed, and the grass carp is fed within 40 days before overwintering. Experiments prove that according to the strategy, fat cells of the grass carps can be proliferated before overwintering, so that the energy utilization mode of the grass carps in the overwintering period is changed, the health condition of the grass carps in the overwintering period is improved, and finally the weight loss of the grass carps is reduced. New opinions and theoretical bases are provided for improving the health condition of the cultured fishes in the overwintering period and reducing fat loss of the cultured fishes in the overwintering period.

The invention discloses application of caffeic acid phenethyl ester to improvement of negative effects of high-carbohydrate feed on fish growth and metabolism, and belongs to the technical field of aquaculture. The caffeic acid phenethyl ester is added into a high-carbohydrate feed formula, so that the metabolic capability of adipose tissues, livers and muscles of the grass carp and the weever on glucose can be remarkably enhanced, ectopic deposition of lipid in the livers and the muscles is relieved, fatty liver diseases are improved, muscle protein deposition is promoted, and the immunity of the grass carp and the weever is improved. The inhibiting effect of the high-carbohydrate feed on the growth performance of the grass carp and the weever is reversed. The invention further provides formulas of the high-carbohydrate feed containing the caffeic acid phenethyl ester for the grass carp and the weever respectively, and data support is provided for research on improvement of the utilization capacity of the grass carp and the weever on the high-carbohydrate feed.

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

The present invention discloses a skin mucus protein molecular marker for accurately quantifying the alkalinity stress level of fish and a detection method thereof, belonging to the technical field of fish alkali tolerance. The present invention aims to solve the problem that there is currently a lack of protein molecular markers for the alkalinity stress level of fish, which in turn affects the efficiency of fish alkali tolerance breeding and selection. The present invention provides a marker for identifying carp fish with alkali tolerance, and the amino acid sequence of the marker is shown in SEQ ID NO.1. It will provide important support for the genetic breeding and selection of alkali-tolerant fish such as grass carp, crucian carp, and Leuciscus waleckii, and further promote the fishery development and utilization of medium and high-saline-alkali waters.

A kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample, the present application relates to the field of microplastic toxicity exposure risk evaluation, specifically relates to a kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample.The present application is to solve the problem that the existing evaluation method is single, cannot comprehensively and accurately evaluate the toxicity exposure risk of microplastic to fish.Method: I, set experimental group;II, input wild grass carp to experimental group, record body length and weight;III, feed cultivation, respectively add corresponding microplastic particles, the control group does not add microplastic particles;IV, combine the weight change of wild grass carp, body length change, feeding rate, mortality and intestinal microbial diversity index for calculation;The higher the value of comprehensive index R, the higher the toxicity risk of microplastic to fish is evaluated.The present application is used to evaluate the toxicity exposure risk of microplastic to fish with wild grass carp as sample.

The application belongs to the technical field of fish variety improvement, and discloses a method for creating gynogenetic grass carp with rapid growth. The method comprises the following steps: screening sexually mature male mirror carp and sexually mature female grass carp with obvious growth advantages and stress resistance, collecting sperm of the mirror carp, diluting the sperm with Hank's solution, inactivating the sperm by ultraviolet irradiation, mixing the obtained inactivated sperm with middle-stage eggs of the grass carp, and then performing cold shock doubling treatment, and adding normal temperature water in batches for hatching to obtain gynogenetic grass carp. The gynogenetic grass carp induced by the mirror carp sperm performs excellently in growth speed and disease resistance, which provides a new method and idea for inheritance improvement of the grass carp, provides more possibilities for screening of excellent varieties, and brings greater economic benefits to the grass carp breeding industry.

The present invention belongs to the field of biotechnology, discloses a keratin filament binding protein of Clonorchis sinensis and its application in preparing a universal oral vaccine for animals and fish, and specifically discloses a fusion protein. The present invention obtains a fusion protein through extensive and in-depth research, uses Bacillus subtilis as a vaccine carrier, induces it to form spores, and then prepares it into a universal oral vaccine for preventing Clonorchis sinensis infection in animals and fish. The oral vaccine can produce a good immune effect in the grass carp, the second intermediate host model of Clonorchis sinensis, and the rat, the worm-retaining host model. After immunization with the oral vaccine, the grass carp, the second intermediate host of Clonorchis sinensis, and the rat, the worm-retaining host, can resist the infection of cercariae or metacercariae of Clonorchis sinensis.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

The invention discloses a method for indicating fish freshness change by adopting cell apoptosis signal molecules and application, and belongs to the technical field of grass carp freshness detection. The invention discloses a method for indicating fish freshness change by adopting cell apoptosis signal molecules, which comprises the following steps: according to the detected contents of cell apoptosis signal molecules Caspase-3, Bax, Bcl-2 and Cyt-c, establishing a corresponding relation model between the content of the cell apoptosis signal molecules and the freshness of grass carp. According to the method, the freshness of the grass carp can be quickly and accurately judged, and a scientific basis is provided for quality control and market supervision of the grass carp. The method can be popularized and applied in actual production and market supervision.

The invention belongs to the technical field of fish genetic breeding in aquaculture industry, and discloses a grass carp intensive high-yield character genome selective breeding method, which comprises the following steps: establishing a grass carp intensive high-yield character prediction model; the establishment of the grass carp intensive high-yield character prediction model comprises the following steps: (1) obtaining a grass carp candidate parent fish single nucleotide polymorphism site; (2) constructing an analysis model for predicting breeding values of candidate parents; and (3) constructing a molecular genetic relationship matrix by using the obtained single nucleotide polymorphic sites, and predicting a genome estimation breeding value of the candidate group in combination with the analysis model in the step (2). The improved variety breeding method based on grass carp genome information can be used for screening excellent parents suitable for intensive breeding, the screened parents are directly used for breeding offspring seeds, and the high-density breeding tolerance of grass carp can be directly improved; and a technical support is provided for improving cultivation of new grass carp varieties suitable for intensive and facility cultivation.

The invention belongs to the technical field of aquaculture, and discloses application of chitosan oligosaccharide in preparation of fish feed for promoting fish growth and improving disease resistance of grass carp bodies. Chitosan oligosaccharide is found to be capable of promoting fish growth and improving the disease resistance of grass carp bodies. In the embodiment, chitosan oligosaccharide is added as an immunopotentiator, so that non-specific antioxidant enzyme expression of the liver of the grass carp can be effectively induced, and the autoimmunity of the grass carp is improved. The chitosan oligosaccharide can improve the intestinal flora structure of the grass carp. In the embodiment, the relative abundance of the clostridium of the 3% chitosan oligosaccharide addition group is remarkably higher than that of the control group, and the 3% chitosan oligosaccharide addition group is the most important bacterial genus of the clostridium, so that the chitosan oligosaccharide has a remarkable effect on improving the intestinal flora structure of the grass carp.

The invention relates to the field of food processing, in particular to a formula and a processing method of first-grade river-crossing fish. The processing method comprises the following steps: selecting main materials, preparing auxiliary materials, preparing seasonings and cooking, namely selecting an aquatic grass carp with the weight of about 800g, scaling the grass carp, removing gills, teeth and viscera, thoroughly cleaning a black film in the belly, cutting the cleaned grass carp, obliquely cutting the grass carp from the head to the tail with the width of about 1.5 cm, and cooking the grass carp with the width of about 1.5 cm; the method comprises the following steps: cutting grass carp into fish meat and fish maw, stopping at the main bone, uniformly smearing cornstarch in each piece of fish meat and fish maw of the cut grass carp, then lifting the fish tail, slightly shaking, removing excessive cornstarch, boiling water in a pot, putting 10g of ginger slices, then lifting the fish tail, putting the fish head into the water, cooking for 8-10 seconds, putting the whole grass carp into the pot, and cooking for 2-3 minutes with soft fire. According to the method, the aquatic grass carps with complete scales, full eyeballs, no whitening, no belly and high activity are selected, so that the freshness and quality of the raw materials are ensured, and a solid foundation is laid for making high-quality dishes.

The present application relates to the technical field of aquaculture, and particularly discloses a method and system for culturing Opsariichthys pachycephalus fry in a ring ditch of a rice field, which comprises the following steps: selecting a rice field and digging a ring ditch around the rice field; digging a fish ditch in the rice field and digging to the fish ditch being connected with the ring ditch; selecting a first-stage culture area in the ring ditch as a culture area for Opsariichthys pachycephalus fry and arranging double-layer blocking nets at two inner walls of the ring ditch; draining and disinfecting the ring ditch; injecting water into the ring ditch; applying amino acids and special organic fertilizer for water quality culture; selecting Opsariichthys pachycephalus fry in a horizontal swimming stage and putting the fry into the water body; feeding the first-stage culture area with the first feed; starting to feed the second feed; injecting water into the first-stage culture area again; adding microbial agents; removing the inner blocking net; pulling the net to count the number of fry and removing the outer blocking net. The present application has the characteristics of reducing stress damage of Opsariichthys pachycephalus, lowering mortality, having less loss and lower breeding cost.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.

The invention provides application of Hnf4alpha or a biological material containing Hnf4alpha in preparation of a product for inhibiting bacteria and / or viruses, and belongs to the technical field of biological medicines. In-vivo and in-vitro experiments show that overexpression of the Hnf4alpha not only can effectively prevent infection of aeromonas salmonicida and grass carp hemorrhagic disease viruses, but also can remarkably inhibit co-infection of the aeromonas salmonicida and the grass carp hemorrhagic disease viruses. The bacterial or virus load after infection is obviously reduced, and the activity of cells and the survival rate of juvenile fish are improved. The invention provides a molecular target for prevention and control of bacterial infectious diseases, virus infectious diseases and mixed infectious diseases of cyprinid fishes.

The invention discloses application of rhizoma osmundae in prevention and treatment of ichthyophthiriasis of aquaculture animals, toxicity experiments of rhizoma osmundae on grass carp kidney cells (CIK) show that the maximum non-toxic concentration of an aqueous extract of rhizoma osmundae on the CIK cells is 1200 [mu] g / mL, and the inhibition of the aqueous extract of rhizoma osmundae on the survival rate of a grazing body of ichthyophthiriasis has dose correlation. When the concentration of the medicine reaches 100 mg / L (mu g / mL), the death rate of the grazing body reaches 100%, and in addition, calculation shows that the median lethal concentration (LD50) of the rhizoma osmundae aqueous extract to the ichthyophthirius multifiliis grazing body is 13.256 mg / L. The application of the rhizoma osmundae in inhibiting the survival rate of the ichthyophthirius multifiliis of the fish under the cell non-toxic concentration is provided for the first time, a novel medicine is provided for preventing and treating ichthyophthirius multifiliis infection of aquaculture animals, and the rhizoma osmundae is a natural medicinal plant, is environmentally friendly and has the advantages of reducing the risk of medicine residues and the like.

The present application belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carp, and specifically discloses a method for improving salt tolerance of fish, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, and placing the fish fertilized eggs in salt water for hatching and domestication; thereafter, the salinity of the water body is increased by 1‰-2‰ every 25-35 days until the salinity is the same as that of the target aquaculture water body. The present application provides a footprint method based on salinity gradual domestication to improve the salt tolerance of fish. The method can effectively activate the expression of tissue osmotic pressure regulation related genes such as gills and kidneys, and ultimately can enable grass carp to adapt to 7‰ water body salinity, breaking the conventional salt tolerance threshold of grass carp. The main performance is that the grass carp still has the characteristics of rapid growth, healthy body, and strong feeding under 7‰ water body salinity.

The application provides a SNP molecular marker for identifying salt tolerance of grass carp and application thereof. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO:1, wherein the base at the 503th position of the sequence is T or C. The SNP molecular marker provided by the application can be applied to rapid identification of salt tolerance of grass carp and assisted breeding of salt-tolerant grass carp. By detecting the SNP molecular marker, salt-tolerant grass carp parents with excellent salt tolerance can be effectively selected under the same breeding conditions, the SNP molecular marker can be effectively used for molecular marker assisted breeding of grass carp, and then the genotypes of the grass carp parents can be identified according to actual breeding requirements, suitable genotype grass carp parents are selected for breeding, and grass carp offspring (fish fry) with high salt tolerance can be obtained, breeding time is saved, cost is low, accuracy is high, the breeding process of the grass carp is accelerated, and the economic benefits of the grass carp in a saline-alkali environment are increased.