81 results about "Grass Carp (fish)" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention discloses sgRNAs of a specific targeting grass carp RIOK3 gene and a construction method and application of an RIOK3 gene knockout cell line, and belongs to the technical field of gene engineering. The invention provides sgRNAs of a specific targeting grass carp RIOK3 gene and a sequence of a primer group, and also provides a method for constructing a large-fragment knockout cell line of the RIOK3 gene in combination with the sgRNAs, and application of the gene knockout cell line in research on an anti-virus infection mechanism of grass carp. The invention discloses the construction of the grass carp gene in-vitro research model by knocking out the antiviral negative regulatory factor RIOK3 of the fish cells by using the CRISPR-Cas9 technology for the first time, provides an in-vitro research material and a new way for the analysis of an antiviral immune regulation molecular mechanism of the grass carp, and particularly has important guiding significance for the research of resisting grass carp reovirus infection.

The invention relates to the technical field of sewage purification, and particularly discloses a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The invention relates to a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The method comprises the following steps: S1, collecting, adjusting and precipitating sewage through a pretreatment unit, and intercepting solid garbage at the same time; s2, introducing the pretreated sewage into a fish-fungus-algae symbiotic unit, putting fungi and algae into the unit, and stocking herbivorous fishes; s3, effluent of the fish-fungus-algae symbiosis unit is guided into a shellfish purification unit, and shellfish is put into the shellfish purification unit; s4, leading effluent of the shellfish purification unit into an aquatic plant purification unit, and planting aquatic plants in the aquatic plant purification unit. The pollution reduction type water quality purification method combining the shellfish and grass carp bacteria and algae can be used for scenes such as village and town domestic sewage, agricultural non-point source pollution and sewage treatment plant tail water, and has the advantages of pollutant reduction and water body self-purification capability reconstruction.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention belongs to the technical field of fish genetic breeding, and relates to a method for screening hemorrhagic disease resistant grass carp and application of the method in hemorrhagic disease resistant breeding of grass carp. Based on a genomics rapid breeding technology, a batch of grass carps capable of resisting the hemorrhagic disease are accurately screened out through the steps of constructing a grass carp experimental group, identifying phenotypes through artificial infection of grass carp hemorrhagic disease viruses, analyzing and identifying genotypes through high-throughput sequencing, carrying out associated screening on SNP sites related to the hemorrhagic disease and the like. The method realizes efficient breeding in a breeding cycle of the grass carp, has the advantages of high efficiency, low cost, genetic stability and the like, can effectively solve the breeding loss caused by hemorrhagic disease of the grass carp, and assists high-quality and green development of aquaculture industry in China.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

A kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample, the present application relates to the field of microplastic toxicity exposure risk evaluation, specifically relates to a kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample.The present application is to solve the problem that the existing evaluation method is single, cannot comprehensively and accurately evaluate the toxicity exposure risk of microplastic to fish.Method: I, set experimental group;II, input wild grass carp to experimental group, record body length and weight;III, feed cultivation, respectively add corresponding microplastic particles, the control group does not add microplastic particles;IV, combine the weight change of wild grass carp, body length change, feeding rate, mortality and intestinal microbial diversity index for calculation;The higher the value of comprehensive index R, the higher the toxicity risk of microplastic to fish is evaluated.The present application is used to evaluate the toxicity exposure risk of microplastic to fish with wild grass carp as sample.

The application belongs to the technical field of fish variety improvement, and discloses a method for creating gynogenetic grass carp with rapid growth. The method comprises the following steps: screening sexually mature male mirror carp and sexually mature female grass carp with obvious growth advantages and stress resistance, collecting sperm of the mirror carp, diluting the sperm with Hank's solution, inactivating the sperm by ultraviolet irradiation, mixing the obtained inactivated sperm with middle-stage eggs of the grass carp, and then performing cold shock doubling treatment, and adding normal temperature water in batches for hatching to obtain gynogenetic grass carp. The gynogenetic grass carp induced by the mirror carp sperm performs excellently in growth speed and disease resistance, which provides a new method and idea for inheritance improvement of the grass carp, provides more possibilities for screening of excellent varieties, and brings greater economic benefits to the grass carp breeding industry.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

The present application relates to the technical field of aquaculture, and particularly discloses a method and system for culturing Opsariichthys pachycephalus fry in a ring ditch of a rice field, which comprises the following steps: selecting a rice field and digging a ring ditch around the rice field; digging a fish ditch in the rice field and digging to the fish ditch being connected with the ring ditch; selecting a first-stage culture area in the ring ditch as a culture area for Opsariichthys pachycephalus fry and arranging double-layer blocking nets at two inner walls of the ring ditch; draining and disinfecting the ring ditch; injecting water into the ring ditch; applying amino acids and special organic fertilizer for water quality culture; selecting Opsariichthys pachycephalus fry in a horizontal swimming stage and putting the fry into the water body; feeding the first-stage culture area with the first feed; starting to feed the second feed; injecting water into the first-stage culture area again; adding microbial agents; removing the inner blocking net; pulling the net to count the number of fry and removing the outer blocking net. The present application has the characteristics of reducing stress damage of Opsariichthys pachycephalus, lowering mortality, having less loss and lower breeding cost.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.

The invention discloses application of rhizoma osmundae in prevention and treatment of ichthyophthiriasis of aquaculture animals, toxicity experiments of rhizoma osmundae on grass carp kidney cells (CIK) show that the maximum non-toxic concentration of an aqueous extract of rhizoma osmundae on the CIK cells is 1200 [mu] g / mL, and the inhibition of the aqueous extract of rhizoma osmundae on the survival rate of a grazing body of ichthyophthiriasis has dose correlation. When the concentration of the medicine reaches 100 mg / L (mu g / mL), the death rate of the grazing body reaches 100%, and in addition, calculation shows that the median lethal concentration (LD50) of the rhizoma osmundae aqueous extract to the ichthyophthirius multifiliis grazing body is 13.256 mg / L. The application of the rhizoma osmundae in inhibiting the survival rate of the ichthyophthirius multifiliis of the fish under the cell non-toxic concentration is provided for the first time, a novel medicine is provided for preventing and treating ichthyophthirius multifiliis infection of aquaculture animals, and the rhizoma osmundae is a natural medicinal plant, is environmentally friendly and has the advantages of reducing the risk of medicine residues and the like.

The present application belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carp, and specifically discloses a method for improving salt tolerance of fish, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, and placing the fish fertilized eggs in salt water for hatching and domestication; thereafter, the salinity of the water body is increased by 1‰-2‰ every 25-35 days until the salinity is the same as that of the target aquaculture water body. The present application provides a footprint method based on salinity gradual domestication to improve the salt tolerance of fish. The method can effectively activate the expression of tissue osmotic pressure regulation related genes such as gills and kidneys, and ultimately can enable grass carp to adapt to 7‰ water body salinity, breaking the conventional salt tolerance threshold of grass carp. The main performance is that the grass carp still has the characteristics of rapid growth, healthy body, and strong feeding under 7‰ water body salinity.

The application provides a SNP molecular marker for identifying salt tolerance of grass carp and application thereof. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO:1, wherein the base at the 503th position of the sequence is T or C. The SNP molecular marker provided by the application can be applied to rapid identification of salt tolerance of grass carp and assisted breeding of salt-tolerant grass carp. By detecting the SNP molecular marker, salt-tolerant grass carp parents with excellent salt tolerance can be effectively selected under the same breeding conditions, the SNP molecular marker can be effectively used for molecular marker assisted breeding of grass carp, and then the genotypes of the grass carp parents can be identified according to actual breeding requirements, suitable genotype grass carp parents are selected for breeding, and grass carp offspring (fish fry) with high salt tolerance can be obtained, breeding time is saved, cost is low, accuracy is high, the breeding process of the grass carp is accelerated, and the economic benefits of the grass carp in a saline-alkali environment are increased.

The invention provides a self-adaptive automatic fish killing device for small and medium-sized grass carps, and relates to the technical field of fresh food processing machinery. Comprising a rack, and a feeding device, an abdominal scale removing device, a roller device, a back scale removing device, a conveying device and a belly opening and viscera removing device which are sequentially arranged on the rack along the technological process. According to the multi-stage self-adaptive flexible scaling system, a floating knife roll is matched with a high-rigidity torsion spring, so that enveloping and scaling of fish bodies with different curvatures are realized; the special-shaped lawn pattern conveying belt module is adopted, so that the problems of slipping and flexible centering clamping of wet and slippery fish bodies are solved; the servo motor is matched with the lifting module to drive the laparotomy tool, and micron-level cutting depth control is achieved. The device is small in size, full-process automation is achieved, and the problems that existing equipment cannot adapt to thick and hard scales of grass carps, gallbladders are prone to being broken when laparotomy is conducted, and manual processing efficiency is low are effectively solved.

This invention relates to an early selection and evaluation method for salt tolerance in grass carp based on serum metabolic markers. The method includes: (1) calculating the salinity sensitivity coefficient based on the serum metabolic markers obtained through screening: SSC = (normal control group index value - salinity treatment group index value) / normal control group index value, the larger the value, the more sensitive the fish; (2) calculating the fuzzy membership index based on the SSC value; (3) calculating the weight coefficient of each serum metabolic marker based on principal component analysis. w =Contribution rate of a single indicator / Cumulative contribution rate of principal components; (4) Calculate the comprehensive evaluation value of grass carp's salinity sensitivity based on fuzzy membership index and serum metabolic marker weight coefficient; (5) Salt tolerance assessment: In the early salt tolerance assessment, the higher the comprehensive evaluation value of salinity sensitivity, the worse the salt tolerance. This method can achieve accurate assessment of grass carp's salinity sensitivity by detecting and analyzing the dynamic changes of specific serum metabolic markers.

The application discloses a feed composition and a preparation method and application thereof, and relates to the technical field of feed additives.The feed composition comprises the following components in parts by weight: 4-6 parts of protease, 1-3 parts of phytase, 5-8 parts of bacillus subtilis, 5-8 parts of bacillus coagulans, 40-60 parts of oil, and 2-4 parts of emulsifier.The specific bacteria and enzymes are used for synergistic effect in the feed composition, so that the prepared feed can not only eliminate the muddy smell of farmed fish, but also reduce the abdominal fat rate of grass carp and improve the tightness of fish meat.

The utility model discloses a flexible cord flap hook, which relates to the field of fishing gear, and comprises a flap component arranged at the bottom of square bait, and a main cord penetrates through the flap component and extends to the bottom of the flap component; the turning plate assembly comprises a turning plate; the plurality of turning plate holes are distributed in a circumferential array shape and are formed in the outer side of the turning plate in a penetrating manner; according to the flexible line flap hook, the sub-lines are arranged to be the long sub-lines and the short sub-lines, the length of the short sub-lines is smaller than the vertical distance from the flap holes connected with the short sub-lines to the long sub-lines, the situation that the adjacent sub-lines are mutually wound when moving in water is reduced, the long sub-lines provide larger-range coverage, meanwhile, a larger fishhook is bound, and the flexible line flap hook is convenient to use. The small fishhook of the short sub-line can not reach the mouth of the big fish after being separated from the square bait aiming at the fish with the big mouth feeding mode of long-distance bait suction, such as silver carp, bighead carp and grass carp, so that the big fish is prevented from escaping when eating the small hook.

The present application relates to long-nosed grass carp breeding technical field, especially green pollution-free long-nossed grass carp breeding method, aiming at the single purification effect of the breeding water in the green breeding process of long-nosed grass carp in the prior art, the present application provides the following scheme, which comprises the following steps: S1, establishing a breeding farm, building suitable breeding ponds according to the breeding scale, and laying bricks and stones or cement hardening treatment on the bottom and side of the breeding pond; S2, establishing a water purification device, installing a certain number of water purification devices in the breeding pond, and regularly starting to purify the breeding water in the breeding pond; S3, prevention and treatment of diseases and insect pests, before putting fish fry, the bottom is comprehensively sprayed with quicklime, the present application not only can conveniently purify the breeding water in the breeding pond, but also can effectively improve the oxygen content in the breeding water in the filtering process, and can also avoid the winding of the water tank on the suction pipe.

This invention discloses an adaptive grass carp descaling and eviscerating integrated machine, realizing the integrated operation of automatic descaling, evisceration, and internal organ removal of grass carp. The integrated machine mainly includes a support module, a descaling module, a conveying module, and an eviscerating module. The support module is equipped with braked casters, ensuring both stable support and flexible movement; the descaling module uses a motor-driven roller brush rotation, combined with adjustable sliding rails to accommodate fish of different sizes; the conveying module uses a motor-driven chain drive to ensure stable transport of the fish; the eviscerating module uses evisceration and eviscerating knives, along with a central guide brace for precise evisceration and efficient removal of internal organs. This invention offers high processing efficiency, thorough descaling, clean evisceration, and a compact structure, suitable for the initial processing of fish in various situations.

The application discloses a formula of compound grass carp feed and a manufacturing process thereof; the feed is made of dry beer lees, fish meal, soybean meal, corn, wheat bran, soybean oil, calcium dihydrogen phosphate, salt, compound multivitamin and compound multimineral as raw materials; the dry beer lees, which is a by-product of daily beer production, has low acquisition cost and high nutritional value, is rich in protein, fat and various nutrients, and is used in the compound grass carp feed, so that the feed production cost can be reduced, the growth and development of the grass carp can be effectively promoted, and more considerable economic benefits can be brought.

The invention belongs to the technical field of aquatic organism breeding, and discloses a method for creating anti-hemorrhagic disease grass carp based on an MVP gene and application. According to the invention, the MVP gene is used as a target gene to carry out gene editing on wild grass carp, an MVP gene knockout F0-generation chimera is selected to carry out a GCRV-II challenge experiment, and the experiment result shows that the GCRV-II virus infection resistance of the grass carp MVP knockout chimera is obviously enhanced, and the grass carp MVP knockout chimera shows less bleeding symptoms and higher survival rate. The correlation between the grass carp MVP gene and the GCRV-II virus is verified for the first time, a brand-new key functional gene target is disclosed for grass carp bleeding disease-resistant breeding, the creation period of disease-resistant grass carp new germplasm is shortened, an ideal new material is provided for grass carp disease-resistant breeding, and the method has a wide application prospect. Technical support is also provided for genetic improvement of virus infection resistance of economic fishes such as grass carp, and the method has important industrial application value.

The invention discloses a DNA molecular module for screening alkali-resistant fast-growing grass carp, a primer pair and application of the DNA molecular module and the primer pair, and belongs to the field of biomolecular markers. In order to solve the problems that the breeding period of grass carp is long and large parent fish is not suitable for alkali resistance test, the invention provides a DNA molecular module for screening alkali-resistant fast-growing grass carp, the DNA molecular module is composed of eight SNP molecular markers, namely SNP01-SNP08 molecular markers (SEQ ID NO.1-8) and primer pairs (SEQ ID NO.9-18) for amplifying the molecular modules. The DNA molecular module and the primer pair provided by the invention are used for identifying the type of the alkali-resistant fast-growing grass carp from the gene level, avoid the occasionality and inaccuracy problems of judging whether the grass carp has the alkali-resistant fast-growing characteristic or not according to the survival state, can be used for early seed selection and shortening the breeding time, and are simple and convenient to operate, relatively high in breeding accuracy and efficiency, and high in practicability. The breeding process of the alkali-resistant fast-growing grass carp germplasm is promoted.

The invention discloses an application of a herba cepbalanoplosis segeti extract in preparation of an inhibitor for fish-derived aeromonas veronii. The herba cepbalanoplosis segeti extract is obtained by extracting a herba cepbalanoplosis segeti sample with water or an organic solvent. The herba cepbalanoplosis segeti extract prepared by the invention has a relatively good inhibition effect on largemouth bass source aeromonas veronii, grass carp source aeromonas veronii and tilapia source aeromonas veronii, and has the characteristics of high efficiency, safety and no toxic or side effect. The herba cepbalanoplosis segeti extract disclosed by the invention is prepared into aquaculture feed, so that bacterial drug resistance caused by excessive use of antibiotics for preventing and treating fish source aeromonas veronii in aquaculture can be avoided, and healthy, stable and sustainable development of aquaculture industry is facilitated.

The invention relates to the technical field of fish disease-resistant breeding, in particular to a method for cultivating a GCRV (Growth CRV Virus)-resistant grass carp strain based on artificial gynogenesis. Comprising the following steps: (1) selection and cultivation of parents; (2) artificial spawning induction; (3) inducing gynogenesis; (4) resistance verification: carrying out a grass carp reovirus attack test on the gynogenetic grass carp fry to verify the GCRV resistance of the gynogenetic grass carp fry; and (5) breeding and expanding propagation: selecting the screened gynogenetic grass carp resistant individuals, cultivating the gynogenetic grass carp resistant individuals as core parents, and applying the core parents to subsequent breeding and expanding propagation of GCRV resistant grass carp groups. The GCRV-resistant grass carp strain cultivated by the method has the advantages of stable resistance, excellent growth and the like, the corresponding cultivation method has the characteristics of short period, high efficiency, strong operability and the like, the problem of grass carp hemorrhagic disease prevention and control can be effectively solved, the healthy and sustainable development of the grass carp breeding industry is guaranteed, and the method has great economic value and social significance.

The utility model discloses screening equipment for crushing grass carp feed, and relates to the technical field of fishery feed production. The device comprises an adjusting mechanism, wherein the adjusting mechanism is used for adjusting the angle of the device; and the repeating mechanism is mounted above the adjusting mechanism, and the repeating mechanism is used for repeatedly turning over the feed after the feed is processed by the repeating mechanism. The stirring plate is arranged, specifically, the connecting plate rotates to drive the stirring plate to conduct circumferential rotation, when the stirring plate conducts circumferential rotation, unqualified materials can be pushed to conduct circumferential rotation, when the stirring plate moves to the upper portion, the stirring plate is in an inclined state, and the unqualified materials can be conveyed to the stirring plate. In this way, the materials pushed by the stirring plates can flow downwards along the inclined faces of the stirring plates and fall to the positions above the crushing rollers, then the materials are crushed through the crushing rollers till unqualified materials are qualified, and the materials do not need to be taken out to be crushed again.

The utility model discloses a lure hard bait capable of being added with food bait, which comprises a bait body, a longitudinal partition plate and a transverse partition plate, and an accommodating space is arranged in the bait body. The containing space in the bait body is divided by the longitudinal partition plate to form a first containing cavity and a second containing cavity. The first accommodating cavity is partitioned by the transverse partition plate to form a bait bin and a water flow bin; a water inlet is formed in the front end of the water flow bin and communicated with the outside, water flow through holes are formed in the outer wall of the water flow bin, and water enters from the water flow bin, is blocked by the longitudinal partition plate, circulates between the bait bin and the water flow bin and then flows out from the water flow through holes. A common lure bait in the market is a fake bait instead of a real traditional bait, the variety of the lure bait is single, and generally, the lure bait is a fierce fish. According to the utility model, the bait is added so as to adapt to various fishes, such as grass carp, silver carp, bighead carp and the like.

The invention relates to the technical field of food processing, in particular to a formula and a cooking process of boiled fish with termitomyces albuminosus and sweet bamboo shoots, and the raw material formula comprises the following components: fresh and alive grass carp, fresh termitomyces albuminosus, fresh sweet bamboo shoots, champignon root, carrot, white bean curd, green bamboo shoot tips, secret soup-stock, ginger, green Chinese onion, lard oil, edible salt, chicken essence and black pepper. Comprising the following steps: 1, pretreating raw materials; step 2, boiling soup-stock and stimulating a base material; step 3, cooking the fish blocks and staple food materials; step 4, adding termitomyces albuminosus and cleaning floating foam; and step five, finally seasoning and taking out of the pot. The invention aims to provide a formula and a cooking process for boiled fish with termitomyces albuminosus and sweet bamboo shoots, and solves the problems that in the processing process of the boiled fish with termitomyces albuminosus and sweet bamboo shoots, the raw material selection of the formula has no clear standard, fish, termitomyces albuminosus and the like have no freshness specifications, and soup-stock lacks the raw material ratio and freshness requirements, so that the finished product has great flavor and quality fluctuation, often has fishy smell or insufficient delicate flavor, and the taste is poor. Cooking depends on manual work, and high-quality taste is difficult to reproduce stably.

The application provides an application of hepatocyte nuclear factor HNF4beta in preparation of a fish disease preventing and / or treating fish biological product, and belongs to the technical field of biotechnology. Through in-vivo experiments, it is found that overexpression of hepatocyte nuclear factor HNF4beta in a fish body can effectively prevent and / or inhibit pathogenic bacteria, especially Edwardsiella tarda, spring viremia of carp virus (SVCV) and grass carp reovirus (GCRV). Meanwhile, the hepatocyte nuclear factor HNF4beta can effectively regulate the immune system, control the occurrence of hepatobiliary syndrome and significantly improve the survival rate of juvenile fish. In addition, the application first proves that HNF4beta can effectively control the hepatobiliary syndrome induced by GCRV infection. The application provides a molecular target for prevention and control of infectious diseases, metabolic diseases and metabolic disease-induced infectious diseases of the Cyprinidae family.

This invention relates to the field of food technology and discloses a grass carp myofibrillar protein / rutin complex, wherein the mass ratio of grass carp myofibrillar protein to rutin is 1:0.01-0.3. This complex is mixed with an aqueous phase and an oil phase under high-speed shear, and after standing, an emulsion gel is obtained. This invention improves the utilization rate and application value of freshwater fish protein. Furthermore, the emulsion gel prepared using the grass carp myofibrillar protein / rutin complex has high stability. Using food-grade materials, it offers advantages such as high safety, wide application range, simple preparation method, and low cost.