146results about How to "Avoid devouring" patented technology

The invention relates to a method for manufacturing a digital PCR (polymerase chain reaction) chip based on a mineral-oil saturated PDMS (polydimethylsiloxane) material. The method is characterized in that the digital PCR chip based on PDMS is prepared from a PDMS monomer of a certain amount of mineral oil (liquid paraffin) and comprises an emulsion droplet generation structure and an emulsion droplet collection structure. After the emulsion droplets are made and collected on the same chip, the emulsion droplets are subjected to PCR amplification on the same chip. The phagocytosis to the oil phase in the digital PCR system by the PDMS of the chip can be avoided, the emulsion droplets can be kept stable during PCR, and the stability of the PCR can be guaranteed. In addition, compared with the existing technology of the digital PCR chip, the method provided by the invention is low in cost, is convenient to operate and has a very wide application prospect.

The invention discloses a preparation method of a polyene-containing taxol nanoparticle mixed micelle preparation and a freeze-drying agent, which prepares docetaxel PLA-PEG nanoparticles or micelle or nanoparticle mixed micelle through a modified solvent evaporation method, takes PLA-PEG copolymer as a carrier, and wraps docetaxel in a PLA hydrophobic core. When in use, the docetaxel PLA-PEG containing long cycle freeze-dried preparation only needs to be added with water and is dissolved, and uniform nanoparticle suspension, micellar solution or mixed micellar nanoparticle suspension can be prepared. The preparation method does not need tween-80 and ethanol solubilization, only takes the biodegradable PLA-PEG as the carrier, and does not contain any surfactant; and compared with the docetaxel injection on sale, the preparation can reduce the toxicity and the adverse reactions of the medicine, and improve the clinical application safety of the medicine.

The invention relates to a mesoporous nano silicon ball compound targeting drug delivery system as well as a preparation method and application thereof. The preparation method of the mesoporous nano silicon ball compound targeting drug delivery system comprises the following steps: 1) preparing amino-functionalized drug loading mesoporous silicon dioxide microspheres; 2) preparing hyaluronic acid-hydrosulphonyl polypeptide-adriamycin (HA-RGD-DOX); 3) preparing mesoporous microsphere-hyaluronic acid-hydrosulphonyl polypeptide-adriamycin--paclitaxel (MSNs-HA-RGD-DOX_PTX); and 4) preparing fluorescent marker modified mesoporous microsphere-hyaluronic acid-hydrosulphonyl polypeptide-adriamycin--paclitaxel compound (MSNs-HA-RGD-DOX-PTX). The mesoporous nano silicon ball compound targeting drug delivery system has the beneficial effects that firstly multi-targeting synergistic drug delivery is realized, multiple tumour cells and tissues can be killed, and reversal drug resistance is good; secondly, blood stability is excellent; thirdly, invisibility, drug release degree and controlled release properties are good; fourthly, in vivo tracing function is good; and fifthly, the mesoporous nano silicon ball compound targeting drug delivery system has good general applicability.

The invention discloses long-circulating solid lipid docetaxel nanoparticles and a preparation method thereof. The long-circulating solid lipid docetaxel nanoparticles comprise the following materialsin therapeutic effective dose: docetaxel, lipid materials, long-circulating auxiliary materials and an emulsifier. The long-circulating solid lipid docetaxel nanoparticles have small particle size, high encapsulation rate and good stability, and not only improve the solubility and the stability of the docetaxel, reduce the toxicity of the docetaxel, but also prolong the circulating time of a medicament in blood, and improve the therapeutic index of the medicament, so that the preparation has the characteristics of low toxicity, low allergy, high efficiency and targeting in clinical application.

The invention discloses a preparation method of RGD-modified ultra-small magnetic iron oxide nanoparticles. The preparation method comprises the following steps: preparing ultra-small magnetic iron oxide nanoparticles by taking ferric acetylacetonate as a reaction raw material and a precursor, taking oleylamine as a surfactant and a reducing agent and taking dibenzyl ether as a solvent; replacing oleylamine molecules wrapped on the surfaces of the nanoparticles by utilizing dopamine-modified HOOC-PEG-COOH to realize PEG-modification of the surfaces of the nanoparticles; and finally, chemically coupling RGD cyclic peptide by virtue of free carboxyl at the tail end of the PEG to obtain the RGD-modified ultra-small magnetic iron oxide nanoparticles. The method of synthesizing the ultra-small magnetic iron oxide nanoparticles has the characteristics of a simple process, a high raw material conversion ratio, strong repeatability and the like. The synthesized magnetic iron oxide nanoparticles have the characteristics of a regular morphology, an ultra-small dimension, good stability, good monodispersity, high biocompatibility, and tumor specific targeting, and the like, and can be used as a T1-weighted imaging high-performance magnetic resonance imaging contrast agent with a tumor active targeting function.

The invention belongs to the field of high-polymer materials and discloses a high-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as a preparation method and an application of the high-polymer vesicle. The high-polymer vesicle is mainly formed through self-assembly of the AIE molecules and amphiphilic block copolymers; the outer layer and the inner layer of a high-polymer vesicle film are hydrophillic layers, each hydrophillic layer is formed by hydrophilic chain sections in the amphiphilic block copolymers, and a film intermediate layer between the outer layer and the inner layer is formed by hydrophobic chain sections in the amphiphilic block copolymers and the AIE molecules. The high-polymer vesicle has high fluorescence intensity and can meet different fluorescent staining demands. The preparation method is simple and feasible, the production efficiency is high, and the repeatability is good. The high-polymer vesicle can be used for related fields of optical bioimaging, biological detection and clinical imaging, high-polymer vesicle research and the like.

The invention belongs to the fields of polymer chemistry and medicinal preparations, and particularly relates to a method for synthesizing an active targeted hyaluronic acid-polylactic acid carrier, a method for preparing an anti-tumor medicinal micelle of the active targeted hyaluronic acid-polylactic acid carrier and an application thereof. By adopting a novel self-assembly technology, amphipathic PEG (polyethylene glycol) block polyester copolymer and tumor targeted ligand hyaluronic acid-polylactic acid copolymer are self-assembled by means of the electrostatic interaction to form a multifunctional composite micelle; the solubility of insoluble tumor medicaments and the drug loading capacity and encapsulation efficiency of water-soluble anti-tumor medicines can be remarkably improved by virtue of the anti-cancer drug-loaded micelle and composite micelle composition, the medicines can be biodegraded in a body, phagocytosis of a reticuloendothelial system (RES) and excretion of a kidney can be avoided. The active targeted hyaluronic acid-polylactic acid carrier has a long-circulating effect, the multifunctional composition has a prominent advantage of tumor active targeting effect, and parameters of pharmacodynamics in vitro and in vivo of the micelle are remarkably superior to those of common anti-tumor injections. Clinically acceptable administration means of the micelle includes injection administration or mucosal administration, and preparations of the micelle can be injection, transfusion, injection lyophilized powder injections or dry powder inhalation.

The invention provides a paclitaxel polymer bonding drug comprising a carboxyl end group contained polymer and paclitaxel esterified and bonded together with the carboxyl end group contained polymer. A preparation method of the carboxyl end group contained polymer comprises the steps of subjecting an aliphatic cyclic ester monomer to ring opening polymerization under the action of a catalyst by taking methoxy polyethylene glycol or polyethylene glycol as an initiator to obtain an amphiphilic polymer; subjecting the amphiphilic polymer and disulfide bond contained diacid to reaction in a first organic solvent under the action of organic alkaline and a condensing agent to obtain the carboxyl end group contained polymer. The solubility of paclitaxel in water can be increased by using the paclitaxel polymer bonding drug; the paclitaxel polymer bonding drug has reduction sensibility so as to be capable of being self-assembled to form a micelle; the paclitaxel polymer bonding drug has favorable hydrophilicity and biocompatibility, so that human reticuloendothelial system phagocytosis in blood circulation can be avoided, and the paclitaxel polymer bonding drug has sufficient time to reach a cancer position through an EPR (Enhanced Permeability and Retention) effect and further enters cells through endocytosis, thus an efficient targeting effect of the drug is achieved.

The invention provides a nanoparticle preparation method. According to the preparation method, a first microemulsion is mixed with a second microemulsion. The first microemulsion contains a surfactant, an organic solvent and a first reactant aqueous solution. The second microemulsion contains a surfactant, an organic solvent and a second reactant aqueous solution. Precipitates can be formed afterthe first reactant contacts with the second reactant. The surfactant in the first microemulsion is the same as that in the second microemulsion, and the surfactant is a mixture of an oil-soluble polyoxyethylene-polyoxypropylene segmented copolymer surfactant and a water-soluble polyoxyethylene-polyoxypropylene segmented copolymer surfactant or a cationic surfactant of cetyl trimethyl ammonium bromide and the like. The nanoparticles provided by the invention have good safety and can obviously raise gene transfection level and promote proteins or drugs to enter into cells.

The invention discloses a wear-resisting corrosion-resistant antibacterial aqueous coating which comprises the following raw materials in parts by weight: 50-55 parts of epoxy resin emulsion, 25-30 parts of acrylic emulsion, 20-28 parts of polystyrene emulsion, 10-15 parts of shell-lac tablet, 40-45 parts of natural latex, 3-6 parts of hydroxyethyl cellulose, 2-5 parts of carboxymethyl cellulose, 1-3 parts of fumed silica, 15-20 parts of styrene-butadiene thermoplastic elastomer, 10-15 parts of polyamide emulsion, 15-20 parts of sodium dodecylbenzene sulfonate, 10-15 parts of lauryl silver sulfate, 5-10 parts of nano-silver, 5-10 parts of titanium nitride, 5-10 parts of aluminum nitride, 10-15 parts of sepiolite powder, 10-15 parts of blast furnace slag powder, 2-4 parts of cerium oxide, 10-14 parts of a defoaming agent, 25-28 parts of a leveling agent, 2-4 parts of an aluminum-zirconium coupling agent TL-3, 3-4 parts of a filming cosolvent. 5-10 parts of a curing agent and 90-105 parts of water.

The invention belongs to the field of a drug preparation, and relates to a docetaxel nanometer lipid carrier and a preparation method. The docetaxel nanometer lipid carrier basically comprises docetaxel, solid state lipid materials, liquid oil, an emulsifier, PEG-modified ester and injection water which are of therapy effective dose. The docetaxel nanometer lipid carrier has stable docetaxel structure, is beneficial to avoiding phagocytosis of reticuloendothelial system in a body, and can be stably stored.

Disclosed are a receptor-mediated quantum dot tracing targeted drug delivery system, a preparation method thereof and application. The targeted drug delivery system comprises polyethylene glycol, hyaluronic acid, quantum dots and melphalan complex which are abbreviated as PEG-HA-QDs-MEL. The PEG is connected with HA amide in a dewatered and condensed manner, the water-soluble CdTe/CdS quantum dots are connected into the HA, and finally, the PEG, the HA and the QDs are prepared with the MEL complex to obtain the targeted drug delivery system. The component content of the PEG-HA-QDs-MEL includes, by weight, 30-100 parts of the MEL, 600-1500 parts of the PEG, 200-500 parts of the HA, 5-50 parts of the CdTe/CdS quantum dots, 20-50 parts of DCC (dicyclohexylcarbodiimide), 1-10 parts of DMAP (dimethylamino-pyridine), 100-500 parts of succinic anhydride, 20-50 parts of NHS, 20-50 parts of EDC (carbodiimide) and 10-100 parts of sodium bisulfite. The targeted drug delivery system is applied to preparing antitumor drugs.

The invention discloses a preparation method of nano micellar solution of polymer with simulated cellulosa membrane structure. The preparation method comprises the following steps: (1) preparing polymer with simulated cell membrane structure with mass ratios of MPC:LMA being 3:7-7:3 and MPC:SMA being 3:7-7:3(2) preparing micellar solution of the polymer; (3) agitating the prepared micellar solution of the polymer at 50-90 DEG C for heat treatment for 5-90min; and (4) cooling and filtering to obtain the nano micellar solution of polymer with simulated cellulosa membrane structure. The invention prepares micellar solution of polymer by simple and convenient direct dissolving method and solvent volatilizing method, and obtains nano micellar solution with compact structure and narrower granulometric distribution after treatment of agitating for 5-90min at 50-90 DEG C.

The invention relates to a multifunctional synergistic pharmaceutical composition based on adriamycin. According to the pharmaceutical composition, natural hydrophobic small molecules having a conjugated structure are covalently coupled with a polysaccharide skeleton to form an anti-angiogenesis drug, the anti-angiogenesis drug is physically mixed with the conjugated structure-modifying mitochondria damage peptide derivative and adriamycin, and the pharmaceutical composition of a nano size is assembled by virtue of various supramolecular driving forces. The pharmaceutical composition has the advantages of simultaneously regulating a tumor micro environment and tumor cells, reversing the anti-apoptosis characteristics of tumor cells, and maximizing the antitumor effect of the adriamycin. Inaddition, the multifunctional synergistic pharmaceutical composition has the advantages of the adriamycin such as high load, high stability and high targeting. The multifunctional synergistic pharmaceutical composition based on the adriamycin is compatible with corresponding medicinal auxiliary materials to prepare antitumoar drug preparations for injection, oral administration or external use. The multifunctional synergistic pharmaceutical composition is prepared by virtue of a multi-component supramolecular combination construction, so that the operation is simple, and the industrialized production is easy to realize.

The invention belongs to the technical field of biological medicines and relates to a paclitaxel-entrapped biodegradable nanocomposite and a preparation method thereof. A paclitaxel polyethylene glycol-poly trimethylene carbonate (PEG-PTMC) nanocomposite is prepared by adopting an emulsification/solvent evaporation method, and by taking a PEG-PTMC copolymer as a carrier, paclitaxel is entrapped in a hydrophobic core of PTMC. The solubility of the paclitaxel can be effectively increased by the prepared paclitaxel PEG-PTMC nanocomposite, and a PEG long chain of a carrier material can take effect of invisibility, and thereby, the phagocytosis of an in-vivo reticulo-endothelial system is avoided. The nanocomposite has a long circulation effect, and the half-life period of the nanocomposite in blood can be prolonged. Moreover, through controlling the grain size of the nanocomposite, a passive targeting effect on a tumor tissue is realized, and thereby, a treatment effect is improved. As the nanocomposite does not contain Cremophor El or ethanol, compared with commercially available paclitaxel injections, the toxic and side effects of the nanocomposite can be reduced, and the safety of the nanocomposite in clinical application is enhanced.

The invention relates to a sophoridine nanoliposome drug and the preparation method. The invention provides a sophoridine nanoliposome drug and the material and the content of the material are as follows: 350-500 compound of HSPC for injection and Vitamin E with a mass ration 9.8: 0.2; 20 to 30 compound of cholesterol and Beta-sitosterol with a mass ratio 1 to 3:1; sophoridine 10 to 25; 125 to 200 compound of dextran 40 and mannitol with a mass ratio 1: 7 to 9; 5 to 10reduced glutathione; and poloxamer F-6835-40. The invention also provides the preparation method of the sophoridine nanoliposome. The drug provided in the invention can be used to treat adenocarcinoma, inflammation, gout, progressive disease of the central nervous system, to relieve pain and treat viral myocarditis.

The invention relates to a sodium ferulate nanomicelle preparation that can be used for intravenous injection, and a preparation method thereof. The sodium ferulate nanomicelle preparation has a plurality of advantages, and the preparation method well controls the quality of products, has good stability and greatly improves the biological availability; the obtained nanomicelle freeze-dried preparation has small particle diameter and is characterized by targeting. The preparation method also has the advantages of being suitable for industrialized production and low production cost.