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High-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as preparation method and application of high-polymer vesicle

A technology of aggregation-induced luminescence and polymer vesicles, which is applied in the direction of medical preparations containing active ingredients, luminescent materials, organic active ingredients, etc., can solve the problems of fluorescence quenching, achieve the protection of genetic material, and the preparation process is simple and feasible , good repeatability

Inactive Publication Date: 2016-04-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages and shortcomings of traditional ACQ fluorescent molecules that will be quenched when the concentration is too high, the purpose of the present invention is to provide a polymer vesicle containing aggregation-induced luminescent molecules

Method used

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  • High-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as preparation method and application of high-polymer vesicle
  • High-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as preparation method and application of high-polymer vesicle
  • High-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as preparation method and application of high-polymer vesicle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) PEG-4000, caprolactone (the molar ratio of PEG to caprolactone is 1:8) and stannous octoate (the amount of stannous octoate is 0.1% of the mass of caprolactone) are blended into toluene, nitrogen Under protection, stir and react at a constant temperature of 160°C for 8 hours (rotational speed is 800r / min), precipitate and filter with excess glacial ether, and obtain the PCL-PEG-PCL triblock copolymer (i.e., PCL 2000 -mPEG 4000 -PCL 2000 );

[0047] (2) Weigh 0.05g triblock copolymer PCL 2000 -mPEG 4000 -PCL 2000 Join in THF of 2g together with 0.005g hexaphenylsilole, mechanically stir 15min (the rotating speed of stirring is 100r / min), obtain macromolecule solution (the consumption of block copolymer and hexaphenylsilole is organic solvent's 2.75wt%);

[0048] (3) Remove the organic solvent in the polymer solution (dry in vacuum at 35°C for 6 hours to completely volatilize the organic solvent) to prepare a composite film of block copolymer and AIE molecules; ...

Embodiment 2

[0050] (1) PEG-2000, caprolactone (the molar ratio of PEG to caprolactone is 1:4) and stannous octoate (the amount of stannous octoate is 0.1% of the mass of caprolactone) are blended into toluene, nitrogen Under protection, stir and react at a constant temperature of 110°C for 48h (rotation speed: 300r / min), precipitate and filter with excess glacial ether, and obtain a PEG-PCL diblock copolymer (i.e., PCL 2000 -PEG 2000 );

[0051] (2) Weigh 0.05g diblock copolymer PCL 2000 -PEG 2000 and 0.001g tetraphenylethylene were added to 1g of acetone / DMAC (volume ratio 2: 1) composite solvent, mechanically stirred for 50min (the rotating speed of stirring was 100r / min), and a polymer solution (block copolymer and tetraphenyl The consumption of phenylethylene is 5.1wt% of organic solvent);

[0052] (3) Under the condition of stirring (stirring speed is 100r / min), take 10mL of deionized water and slowly add it dropwise into the polymer solution to obtain a W / O composite emulsion; p...

Embodiment 3

[0054] (1) Take PEG-5000, lactide (the molar ratio of PEG to lactide is 1:0.2) and stannous octoate (the amount of stannous octoate is 0.05% of the mass of lactide) is added in toluene solvent, nitrogen atmosphere Stir at a constant temperature of 150°C for 18 hours (350r / min), then precipitate in excess cold ether, and filter with suction to obtain the PEG-PLA block copolymer (PLA 5000 -PEG 5000 );

[0055] (2) Weigh 0.05g diblock copolymer PLA 5000 -PEG 5000 and 0.001g tetraphenyl-1,4-butadiene were added into 1g of THF, mechanically stirred for 5min (the rotating speed of stirring was 700r / min), and a polymer solution (block copolymer and tetraphenyl-1 , 4-butadiene consumption is 5.1wt% of organic solvent);

[0056] (3) Under the condition of stirring (stirring speed is 100r / min), slowly add 10mL of deionized water into the polymer solution dropwise to prepare W / O emulsion, and then continue mechanical stirring for 50h (stirring speed (300r / min) to make the organic so...

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Abstract

The invention belongs to the field of high-polymer materials and discloses a high-polymer vesicle containing AIE (aggregation-induced emission) molecules as well as a preparation method and an application of the high-polymer vesicle. The high-polymer vesicle is mainly formed through self-assembly of the AIE molecules and amphiphilic block copolymers; the outer layer and the inner layer of a high-polymer vesicle film are hydrophillic layers, each hydrophillic layer is formed by hydrophilic chain sections in the amphiphilic block copolymers, and a film intermediate layer between the outer layer and the inner layer is formed by hydrophobic chain sections in the amphiphilic block copolymers and the AIE molecules. The high-polymer vesicle has high fluorescence intensity and can meet different fluorescent staining demands. The preparation method is simple and feasible, the production efficiency is high, and the repeatability is good. The high-polymer vesicle can be used for related fields of optical bioimaging, biological detection and clinical imaging, high-polymer vesicle research and the like.

Description

technical field [0001] The invention belongs to the field of polymer materials, and relates to a polymer vesicle containing aggregation-induced luminescence molecules and a preparation method and application thereof. Background technique [0002] Optical bioimaging technology has good spatial and temporal resolution, and is a research hotspot in the fields of biomedicine and molecular biology. The development of fluorescence-based optical bioimaging technology provides a direct visualization tool for the observation of biological processes and clinical imaging; it has great potential in tumor detection, gene expression monitoring, protein molecular detection, drug receptor localization, drug screening, and drug efficacy evaluation. Great application potential. Traditional fluorescent chromophores have the advantages of high sensitivity, high selectivity, a variety of measurement parameters can be set, and various detection methods; however, their fluorescence is weakened or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L67/04C08J3/00C09K11/02C09K11/06A61K9/50A61K9/51A61K31/704A61K49/00A61P35/00G01N21/64
CPCA61K9/5031A61K9/5153A61K31/704A61K49/0091A61K49/0093C08J3/00C08L67/04C09K11/025C09K11/06G01N21/6486C08J2367/04C09K2211/1011C09K2211/1007C09K2211/1037A61K49/00A61K9/50C09K11/02G01N21/64
Inventor 王林格李卫昌秦安军唐本忠潘凌翔
Owner SOUTH CHINA UNIV OF TECH
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