35 results about "Protein digestion" patented technology

Described are an apparatus for generating and collecting nanospray reactive droplets and a method for analyzing a protein digestion. The apparatus includes a capillary, counter electrode, collection vessel, electrostatic lenses, and voltage control module. The capillary receives a mixture that includes a protein and an enzyme and has an outlet disposed in the aperture to dispense the mixture. The collection vessel has a concave surface to receive a nanospray plume emitted from the capillary outlet. The counter electrode and electrostatic lenses are disposed along a nanospray path defined between the capillary outlet and the collection vessel. Each electrostatic lens is formed of an electrically conductive plate having an aperture therein to pass a nanospray reactive plume. The voltage control module is in electrical communication with and is configured to independently control the voltage of the capillary, the counter electrode, the collection vessel and each of the electrostatic lenses.

The invention discloses an SNP molecular marker located on a pig chromosome 6 and related to nitrogen apparent digestibility and application of the SNP molecular marker, the site of the SNP molecular marker is rs3475771848 and corresponds to the 35437413bp site on a chromosome 6 of an international pig reference genome version 11.1, and T gt exists at the site; a mutation. The SNP molecular marker provided by the invention is remarkably related to the nitrogen apparent digestibility character of the pig, the identification of the nitrogen apparent digestibility character of the pig can be realized by identifying the genotype of the SNP molecular marker, and the identification of the nitrogen apparent digestibility character of the pig can be realized by breeding the pig with the genotype of the SNP molecular marker TA. The nitrogen apparent digestibility of offspring can be improved, and the protein digestion and utilization capability and feed conversion efficiency of the offspring can be improved, so that the breeding process of pigs is accelerated, and genetic improvement of the pigs is realized.

The invention discloses a kit and a method for extracting nucleic acid from an ophthalmic sample and application of the kit and the method in metagenome sequencing. A method of extracting nucleic acid from an ophthalmic sample includes extracting nucleic acid from the ophthalmic sample using a sample processing system comprising the ophthalmic sample, a carrier RNA, protease K, and a lysis reagent. According to the method, a carrier RNA is added to prevent loss of trace nucleic acid, high-concentration protease K is adopted to enhance digestion of protein, and a chemical reagent is adopted to lyse cells, so that excessive damage and degradation of nucleic acid and low-volume elution caused by severe oscillation of mechanical wall breaking are avoided, and the final concentration of nucleic acid is improved.

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.

Conventional expanded microscopy (ExM) is improved upon to show reliable and reproducible 4-fold and 12-fold modified expansion microscopy (mExM). Kits and methods are disclosed to achieve these improvements. The kits can include a sealable mold and instructions for using it in the improved methods. The kits can include a protein digestion buffer including sodium dodecyl sulfate and instructions for using the buffer in the improved methods. The improved methods can include a simplified approach to executing oxygen-sensitive reactions within the methods. The improved methods can include improved protein digestion chemistries, which can facilitate the improvements in reliability and reproducibility.

This invention discloses a slow-digesting quinoa flour-concentrated milk protein composite gel food and its preparation method, belonging to the field of food processing technology. The invention first protects the composition as a gel food prepared from raw materials containing concentrated milk protein and quinoa flour; the preparation includes premixing quinoa flour and a portion of concentrated milk protein, hydrating them to obtain a premix with a starch-protein network, adding the remaining raw materials, stirring to form a slurry, and then shaping it. The gel food of this invention constructs a dense and continuous starch-protein network. This network, on the one hand, acts as a physical barrier to prevent effective contact between gastrointestinal digestive enzymes and internal proteins, maintaining the stability of the protein's secondary structure, thereby slowing down the rate of protein digestion and hydrolysis in the gastrointestinal tract to achieve slow digestion; on the other hand, it directly improves the product's hardness, chewiness, and other textural parameters, enhances water-holding capacity, reduces water separation rate, optimizes color and flavor, and achieves a synergistic improvement in multiple indicators of edible quality.

The utility model belongs to the field of detection equipment. An exhaust port of an exhaust collector on a digestion furnace body is communicated with an exhaust absorption unit through an exhaust pipeline, the bottom of the exhaust pipeline of the exhaust absorption unit is inserted below the liquid level of a water absorption device, and the liquid level of the water absorption device is communicated below the liquid level of a sodium hydroxide solution absorption device through a gas communicating pipe. The sodium hydroxide solution absorption device is communicated with an exhaust pipe, a suction pump is arranged on the exhaust pipe, a sulfur dioxide detector and a waste discharge valve are installed at the front end and the rear end of the suction pump respectively, a bypass re-absorption pipe is arranged between the waste discharge valve and the suction pump, a gas return control valve is arranged on the re-absorption pipe, and the re-absorption pipe is communicated with a tail gas pipeline. The two-stage absorption device is arranged, the absorption effect is good, the reagent cost is low, tail gas subjected to two-stage absorption is detected through the sulfur dioxide detector, zero-pollution emission is truly achieved, the tail gas is subjected to closed continuous treatment from the exhaust gas collector to emission, the physical health of experimenters is guaranteed, and environmental pollution is reduced.

The utility model relates to the technical field of protein detection, and provides a protein detection workstation which comprises a workbench, a digestion tube placing rack arranged on the workbench, a protein pretreatment device, a protein digestion device, a sample boat placing rack, an azotometer and a manipulator, the digestion tube placing rack, the protein pretreatment device, the protein digestion device, the sample boat placing rack and the azotometer are at least arranged on two sides of the manipulator; the manipulator can move the digestion tube from the digestion tube placing rack to the protein pretreatment device, transfer the digestion tube from the protein pretreatment device to the protein digestion device, transfer a digested protein detection solution in the digestion tube into the sample boat, and transfer the sample boat into the azotometer. According to the utility model, the digestion tube can be moved and the protein detection solution can be transferred by utilizing the manipulator according to the requirements, so that automatic operation is realized, manual operation is reduced, and the detection cost is reduced.

A method for characterizing proteins, including steps of (a) detecting a plurality of proteins, wherein individual proteins of the plurality are associated with unique identifiers, wherein the detecting distinguishes the identities of the individual proteins and the unique identifiers associated with the individual proteins; (b) digesting the proteins to form peptides, wherein the peptides from each protein are associated with the unique identifiers for the respective individual protein; (c) detecting the peptides and associated unique identifiers, wherein the detecting distinguishes characteristics of individual peptides, and wherein the detecting distinguishes unique identifiers associated with the individual peptides; and (d) correlating characteristics detected in step (c) with individual proteins detected in step (a) based on the unique identifiers associated with the individual proteins and the peptides.

The invention relates to the technical field of protein digestion, in particular to an activity induced protein digestion efficiency optimization method and system. According to the method, diet data and protein data of multiple target users are collected, all data information is preprocessed to obtain preprocessed information, then an optimization model is established, representative users are screened out from the multiple target users based on the diet data through the optimization model, and user experience is improved. And respectively establishing a representative protein standard for each representative user based on the protein data of the representative user, performing multi-classification learning on the optimization model in combination with the protein standards to obtain a trained optimization model, and finally collecting the real-time diet data and the real-time protein data of the real-time user to obtain the real-time diet data and the real-time protein data of the real-time user. According to the method, the real-time protein data of the real-time user is optimized based on the real-time protein standard, the representative protein standard is established, so that the optimization model can directly adjust the digestion efficiency of the induced protein of the user based on the representative protein standard, and the adjustment efficiency is improved.

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

The application provides a taste-improved linalool embedded microcapsule feed additive and a preparation method thereof. The additive is a microcapsule composed of core material linalool and wall material, and the wall material comprises raw material matrix, black soldier fly larvae powder, fly maggot powder, edible gelatin, edible lactose and emulsifier; the fermented raw material matrix is prepared from bean curd residue, peanut cake, wheat bran and cassava residue through co-fermentation of lactic acid bacteria and yeast. The preparation method comprises the following steps: mixing the raw material matrix and wall material components to prepare a wall material aqueous solution, adding linalool emulsion, and then performing composite coagulation, glutamine transaminase cross-linking solidification and spray drying to obtain the product. In the application, the anti-nutritional factors of raw materials are removed through fermentation, the protein digestion and utilization rate is improved, the pungent smell of linalool is shielded with the help of gelatin and lactose, the volatilization and oxidation of linalool are slowed down, the feed palatability is greatly improved, the product stability is good, and the product can be widely applied to livestock and poultry breeding feed.

The invention discloses anti-vomiting hydrolyzed whey protein and a preparation method thereof, and belongs to the technical field of food processing. The product provided by the invention reduces the digestion burden of protein and relieves the symptom of vomiting. According to the method disclosed by the invention, protein sequences are mutually crosslinked by virtue of a treatment mode of first fibration, second polymerization and final hydrolysis, and a hydrolyzed polypeptide sequence is changed, so that the abundance of the polypeptide SLAMAASDISLA in a hydrolysate is improved. The polypeptide has the characteristics of being easy to recognize by intestinal peptidase and relatively high in PepT1 affinity, the bioavailability of protein is improved, and the deamination proportion of unutilized amino acid is reduced, so that substances generating nitrogen-containing toxoid are reduced, the stimulation of toxin accumulation on gastrointestinal mucosa is relieved, and the symptoms of nausea, vomiting and the like of a user are avoided.

The invention discloses an RNA co-immunoprecipitation kit for specific binding of prion protein in muscle cells and an RNA extraction method, and relates to the technical field of bioengineering. According to the present invention, the specific co-precipitation kit preparation and the specific extraction steps comprise cell lysis, cell lysis solution-antibody-magnetic bead incubation, protein digestion and RNA extraction, such that the high purity and the sufficient amount of the enriched RNA are successfully ensured so as to completely meet the strict requirements of the subsequent RNA-seq sequencing. The method overcomes the inherent defects of the existing RIP technology in the research of non-classical RNA binding proteins, realizes the optimization of the whole process from high-specificity enrichment to high-quality sequencing sample preparation through the integrated innovation of methodology and the kit, has the outstanding advantages of strong specificity, high sensitivity, good repeatability, simple operation and the like, and has a wide application prospect in the research of non-classical RNA binding proteins. And remarkable technical progress is achieved, and a positive application effect is generated.

The invention relates to an RNA (Ribonucleic Acid) co-immunoprecipitation-based RNA binding protein aggregate molecular function detection method, which comprises the following steps: S1) RNA co-immunoprecipitation: firstly, splitting tissues or cells, then cleaning by using magnetic beads to remove a splitting solution, and then preparing a detection sample; s2) protein and RNA detection: firstly carrying out semi-denaturation gel electrophoresis on the sample, confirming the IP efficiency of the sample, and then recovering RNA on the membrane; s3) releasing RNA from the membrane: incubating by adopting a prepared PK buffer solution to perform protein digestion so as to extract RNA, measuring the concentration of RNA, and performing reverse transcription to obtain RNA formed by combining a DAZL polymer and a DAZL monomer; s4) performing qPCR (quantitative polymerase chain reaction) detection on a target target: performing function detection, and verifying whether RNA (ribonucleic acid) exists in the aggregate formed by combining the DAZL polymer and the monomer. The method is simple, efficient and accurate, and provides a more convenient platform for functional research of the polyamyloid protein.

The invention discloses high-protein coarse cereal nutritional meal replacement powder and a preparation method thereof, and relates to the field of food processing, the high-protein coarse cereal nutritional meal replacement powder comprises a continuous gel matrix phase formed through mineralization crosslinking and an adsorption slow-release phase dispersed in the continuous gel matrix phase; the continuous gel matrix phase is formed by reacting pea protein, enzymolysis oat beta-glucan and a compound mineralizing agent; the adsorption slow-release phase is composed of porous starch loaded with hydrolyzed whey protein peptide and flavor substances; the composite mineralizer comprises calcium salt and magnesium salt; according to the nutritional meal replacement powder, a three-dimensional gel network with a loose structure and strong hydrophilicity is formed through a coupling technology of constructing a continuous gel matrix phase through mineralization cross-linking and forming a dispersed slow-release phase through porous adsorption, disordered hydrophobic aggregation of protein and fibers is efficiently prevented, hydration reconstruction can be rapidly performed during brewing, and in the digestion process, the nutritional meal replacement powder has the advantages that the dissolution rate is high; the loose gel network provides a smooth permeation path for digestive enzymes, and the protein digestibility is high.

The invention discloses an SNP molecular marker located on a pig chromosome 9 and related to nitrogen apparent digestibility and application of the SNP molecular marker, the site of the SNP molecular marker is rs337541051 and corresponds to the 133972770bp site on a chromosome 9 of an international pig reference genome version 11.1, and Cgt exists at the site; t mutation. The SNP molecular marker provided by the invention is remarkably related to the nitrogen apparent digestibility character of the pig, the identification of the nitrogen apparent digestibility character of the pig can be realized by identifying the genotype of the SNP molecular marker, and the identification of the nitrogen apparent digestibility character of the pig can be realized by breeding the pig with the genotype of the SNP molecular marker CC. The nitrogen apparent digestibility of offspring can be improved, and the protein digestion and utilization capability and feed conversion efficiency of the offspring can be improved, so that the breeding process of pigs is accelerated, and genetic improvement of the pigs is realized.

The application discloses a preparation method of a lycopene and konjac glucomannan gel for improving protein digestion, and belongs to the technical field of food processing. In view of the technical problems that the dense network of polysaccharide inhibits protein digestion and high-concentration lycopene is prone to self-agglomeration, leading to unstable antioxidant effect in the prior art, the problems are solved through the following scheme: myofibrillar proteins and konjac glucomannan are mixed in a buffer solution to perform first hydration, and a protein-polysaccharide re-dissolution is obtained; then, a heat-labile protease is added to perform second hydration, and a protein-polysaccharide-enzyme re-dissolution is obtained; lycopene is mixed with edible oil to obtain a lycopene-oil phase; the oil phase is mixed with the enzyme re-dissolution to be homogenized to prepare an oil-in-water emulsion; finally, the temperature is increased from 25 DEG C to 80 DEG C at a rate of 1-3 DEG C / min and is kept for 20-40 min, and then the temperature is cooled to 2-6 DEG C and is kept for 12-24 h to perform heat-induced gelation. The application is mainly used for preparing a gel with high gel strength, good digestion performance and antioxidant stability.

Provided herein are methods of preparing a polypeptide sample and methods of protein analysis. Also provided herein are compounds of Formula (I), (II), and (III), and use thereof, including in methods of protein digestion.

The application discloses a kind of suitable for honey bag glider whole physiological stage intestinal homeostasis type precision nutrition formula and preparation method thereof, belong to special pet feed technical field.The formula includes basic nutrition composition 30~80 parts by weight, intestinal homeostasis composition 5~25 parts, functional additive 1~10 parts;Wherein the basic nutrition composition is with enzymolysis animal protein, insect protein, compound carbohydrate and functional oil as core, precisely match the nutritional requirement of honey bag glider whole physiological stage;Intestinal homeostasis composition adopts "microcapsule probiotic + compound prebiotic + intestinal mucosa repair factor" trinity system, and cooperatively constructs stable intestinal microecology.Preparation method adopts low-temperature conditioning granulation + probiotic post-spraying process, and guarantees the activity of functional components.The application realizes whole physiological stage free replacement grain feeding, significantly improves protein digestion rate, reduces intestinal disease incidence, with outstanding novelty, creativity and industrial practicality.

The application discloses a kind of rich in wheat oligopeptide compressed dry food digestion performance evaluation method and device, the method comprises: using in vitro dynamic human gastrointestinal digestion system, not containing wheat oligopeptide compressed dry food and rich in wheat oligopeptide compressed dry food are respectively simulated digestion processing, respectively measured to obtain first measurement index set and second measurement index set;Each type of measurement index set, including gastric emptying curve, protein digestion rate sequence, amino acid composition value sequence, fat bioavailability sequence;The gastric emptying curve includes several discrete points;The first measurement index set and second measurement index set are preprocessed, and obtain preprocessed index information set;The preprocessed index information set is evaluated and processed, and obtains the digestion performance evaluation value of rich in wheat oligopeptide compressed dry food.

A method for improving digestion performance for proteins in food, and a protein composition and food containing same. According to the method, the content of α-lactalbumin in food is controlled to be 10.5% to 51.0%, and the content of β-casein is controlled to be 10.0% to 40.0%. Said contents are calculated on the basis that the total protein content in the food is 100%.

According to the method, firstly, a composite gel system formed by mixing gellan gum and konjac glucomannan according to a specific proportion and stabilizing sunflower seed oil in cooperation with whey protein is constructed, a three-dimensional network structure with a synergistic enhancement effect is formed, and the water binding capacity and swallowing suitability of a product are remarkably improved; secondly, the surimi is subjected to enzymolysis softening by adopting alkaline protease at a proper temperature, so that the texture hardness is effectively reduced, and the protein digestion and absorption rate is improved; on the basis, potassium chloride partially replaces sodium chloride, curdlan is combined to construct a gel network, and multiple technologies such as microwave curing and high hydrostatic pressure sterilization are cooperated, so that the sodium ion release and distribution uniformity is systematically enhanced, and good salty taste perception is maintained while the salt is reduced by 30% or above; according to the method, the swallowing safety, the flavor fullness and the storage stability of the product are perfectly considered while the sodium content is remarkably reduced, and an ideal dietary choice is provided for people with dysphagia.

The application discloses a milk powder formula containing hydrolyzed whey protein and calcium-phosphorus matching and a preparation method, relates to the technical field of milk powder preparation, and contains core components such as whole milk powder, skim milk powder, hydrolyzed whey protein and compound adjuvants according to the percentage by weight, the molar ratio of calcium to phosphorus is 1.8-2.0:1, the milk solid content is greater than or equal to 70%, and the product meets the requirements of the national standard GB19644-2024, the preparation method comprises the following steps: raw material pretreatment, step-by-step dry powder mixing, optional dry granulation, drying and screening and the like, and the whole process is suitable for the dry production process of a factory. Through the cooperation of accurate formula design and fine dry process, the application improves the protein digestion and absorption rate and the calcium absorption and utilization rate, guarantees the stability of functional components, has strong product compliance and high practicability, can provide targeted nutritional support for the growth and development of children and adolescents, and has a wide application prospect.

This invention provides a method for identifying characteristic flavor compounds in pork based on multi-omics and machine learning. By integrating two-dimensional gas chromatography-time-of-flight mass spectrometry, liquid chromatography-mass spectrometry, and transcriptome sequencing technologies, volatile flavor compounds, metabolites, and gene expression data are acquired simultaneously. Based on relative odor activity value calculations and combined with machine learning algorithms, key flavor biomarkers such as γ-dodecanolactone, 2,3-butanedione, and 2-pentylfuran are screened. Furthermore, a multi-omics regulatory network is constructed to identify core regulatory genes. CARNS1 and SLC1A This invention reveals that key flavor compounds in pork influence flavor precursor synthesis by regulating histidine metabolism and protein digestion and absorption pathways. It systematically analyzes and precisely identifies these compounds, elucidating their formation mechanisms at the molecular level. This provides reliable technical solutions and decision-making basis for the breeding of high-quality pork breeds, optimization of farming processes, and targeted regulation of flavor quality.

The application discloses a kind of compressed dry food digestion performance prediction method and device, the method comprises: the component information set of compressed dry food is obtained by acquisition;The component information set includes protein content information, mineral content information, starch content information and fat content information;The preset digestion performance prediction model is trained and handled, and the digestion performance prediction model that training is completed is obtained;Using the digestion performance prediction model that training is completed, the component information set of compressed dry food obtained by acquisition is fused and predicted and handled, and the digestion performance prediction information set is obtained;The digestion performance prediction information set includes protein content prediction value, amino acid content prediction value, apparent viscosity prediction value, protein digestion rate prediction value.

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

The application discloses a yeast protein and citrus fiber functional assembly based on a high-pressure homogenization process and a preparation method thereof, and belongs to the field of novel functional feed.The yeast protein and the citrus fiber are subjected to high-pressure homogenization treatment in a liquid phase, so that the small particles of the yeast protein are uniformly adsorbed on the porous structure of the citrus fiber, and when the cumulative particle size distribution number of the self-assembly reaches 90%, the particle size (D90) is less than 500 nm, which is in the submicron level. Subsequently, the biological activity is maximally retained through freeze-drying, and finally, the final product is obtained through high-speed mixing and granulation. Compared with a traditional physical mixture, the assembly prepared by the method has higher protein digestion rate and anti-inflammatory activity, can effectively replace a traditional feed protein source in a specific proportion, and significantly improves the intestinal health of a colonitis model animal. The application realizes dual innovation of a preparation process and application function, and has extremely high industrial application value.

The invention belongs to the field of health food, and discloses a black tea and walnut peptide compound, a preparation method thereof and application of the black tea and walnut peptide compound in relieving immunosuppression induced by cyclophosphamide. Researches find that FT-WP treatment significantly reverses weight, thymus and spleen indexes of mice induced by cyclophosphamide, improves balance of jejunum CD4 + T cell subsets, and balances levels of cytokines and immune globulins. In addition, the research shows that FT-WP affects metabolic pathways such as metabolism of glycine, serine and threonine, metabolism of pyrimidine, digestion and absorption metabolism of protein and the like and regulation of metabolites such as choline, trimethylamine-N-oxide, prothioconazole, stearoyl carnitine, prostaglandin F2. Beta and the like. The black tea and walnut peptide composite food can relieve immunosuppression induced by cyclophosphamide and regulate abnormal serum metabolism.

The utility model relates to the technical field of protein detection, and provides a protein digestion device which comprises a digestion furnace, a digestion frame and a first driving piece, the digestion frame is arranged above the digestion furnace, and a plurality of digestion pipe clamping holes are formed in the digestion frame; the first driving part comprises a first driving end and a first fixing end, the first driving end is connected with the digestion frame, and the first fixing end is connected with the digestion furnace so as to drive the digestion pipe to be inserted into the digestion furnace or leave the digestion furnace. The digestion pipes are driven by the first driving piece to be simultaneously inserted into or separated from the digestion furnace, so that automatic operation is realized, and the digestion efficiency is improved.