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45 results about "Protein digestion" patented technology

Protein digestion occurs as a result of the hydrolysis of the peptide bonds that bind the individual amino acids in the polypeptide chain. These reactions are catalyzed by enzymes called proteases. ... Two stages are involved in the digestion of proteins: the first occurs in the stomach and the other in the in the duodenum, the first part of the small intestine.

Enzyme-containing postbiotic coating feed additive for pets and preparation method of enzyme-containing postbiotic coating feed additive

The invention relates to the technical field of pet feed additives, and discloses a pet coated enzyme-containing metagen feed additive and a preparation method thereof, the additive comprises a core particle formed by mixing a metagen and a slow-release coated compound enzyme, and an enteric layer coated outside the core particle. The preparation method comprises the following steps: respectively preparing the metagen and the slow-release coated compound enzyme, mixing the metagen and the slow-release coated compound enzyme to prepare core particles, and then carrying out enteric coating on the core particles. The activity of functional components is protected through a double coating structure, so that the functional components are released in an intestinal tract in a targeted manner and have a synergistic effect. The additive can effectively improve pet protein digestion, regulate intestinal microenvironment, remarkably proliferate beneficial bacteria such as lactobacillus and clostridium praeparatum and inhibit escherichia coli, so that soft stool is reduced, and intestinal health of pets is maintained.
Owner:ANHUI MAITEBAO BIOENGINEERING CO LTD

Nanospray reactive sprayer

Described are an apparatus for generating and collecting nanospray reactive droplets and a method for analyzing a protein digestion. The apparatus includes a capillary, counter electrode, collection vessel, electrostatic lenses, and voltage control module. The capillary receives a mixture that includes a protein and an enzyme and has an outlet disposed in the aperture to dispense the mixture. The collection vessel has a concave surface to receive a nanospray plume emitted from the capillary outlet. The counter electrode and electrostatic lenses are disposed along a nanospray path defined between the capillary outlet and the collection vessel. Each electrostatic lens is formed of an electrically conductive plate having an aperture therein to pass a nanospray reactive plume. The voltage control module is in electrical communication with and is configured to independently control the voltage of the capillary, the counter electrode, the collection vessel and each of the electrostatic lenses.
Owner:WATERS TECHNOLOGY CORP

Apparatus and method for mechanically mixing meat products

To provide an apparatus 1 having at least one tool 2 for producing protein decomposition products in a meat material G.SOLUTION: A tool 2 forms a mixing chamber 3 for a meat material G. The mixing chamber 3 includes opposing chamber walls 4a, 4b that are rotatable relative to each other and control a force applied to the meat material G positioned between them. The invention also relates to a method for producing protein decomposition products in the meat material G.SELECTED DRAWING: Figure 1
Owner:EVERHARD GAMBEHER

A method for extracting DNA from kappaphycus alvarezii

PendingCN122750672ATE bufferLysis buffer
A method for extracting DNA from Kappa longicornis, relating to the field of molecular biology, includes the following steps: grinding a Kappa longicornis sample into powder; adding lysis buffer preheated to 60–65°C to the powder and incubating at 60–65°C; adding Proteinase K for protein digestion; adding RNase A for RNA digestion; extracting with a mixture of chloroform and isoamyl alcohol, centrifuging, and collecting the supernatant; adding pre-cooled isopropanol to the supernatant for precipitation, centrifuging, and obtaining the DNA precipitate; washing and drying the DNA precipitate; and dissolving the DNA in TE buffer or nuclease-free water. The beneficial effects of this invention are as follows: by optimizing the lysis buffer and combining it with stepwise enzymatic hydrolysis and organic extraction processes, polysaccharides and pigments of *Kappa longicornis* can be efficiently removed, and the extracted DNA concentration is >300 ng / μL, with a purity of A260 / A280≈1.76 and A260 / A230≈2.21, meeting the requirements of downstream molecular experiments; at the same time, reduced glutathione is used instead of 2-mercaptoethanol, making the operation safe and odorless, with low reagent costs, and suitable for batch sample processing.
Owner:SHANTOU UNIV

Application of SNP molecular marker located on pig chromosome 6 and related to nitrogen apparent digestibility

The invention discloses an SNP molecular marker located on a pig chromosome 6 and related to nitrogen apparent digestibility and application of the SNP molecular marker, the site of the SNP molecular marker is rs3475771848 and corresponds to the 35437413bp site on a chromosome 6 of an international pig reference genome version 11.1, and T gt exists at the site; a mutation. The SNP molecular marker provided by the invention is remarkably related to the nitrogen apparent digestibility character of the pig, the identification of the nitrogen apparent digestibility character of the pig can be realized by identifying the genotype of the SNP molecular marker, and the identification of the nitrogen apparent digestibility character of the pig can be realized by breeding the pig with the genotype of the SNP molecular marker TA. The nitrogen apparent digestibility of offspring can be improved, and the protein digestion and utilization capability and feed conversion efficiency of the offspring can be improved, so that the breeding process of pigs is accelerated, and genetic improvement of the pigs is realized.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Kit and method for extracting nucleic acid from ophthalmic sample and application of kit and method in metagenome sequencing

The invention discloses a kit and a method for extracting nucleic acid from an ophthalmic sample and application of the kit and the method in metagenome sequencing. A method of extracting nucleic acid from an ophthalmic sample includes extracting nucleic acid from the ophthalmic sample using a sample processing system comprising the ophthalmic sample, a carrier RNA, protease K, and a lysis reagent. According to the method, a carrier RNA is added to prevent loss of trace nucleic acid, high-concentration protease K is adopted to enhance digestion of protein, and a chemical reagent is adopted to lyse cells, so that excessive damage and degradation of nucleic acid and low-volume elution caused by severe oscillation of mechanical wall breaking are avoided, and the final concentration of nucleic acid is improved.
Owner:SANSURE BIOTECH INC

Aquatic protein feed and preparation method thereof

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.
Owner:YUEYANG YUMEIKANG BIOTECHNOLOGY CO LTD

Functional gleditsia sinensis fermented royal noodles and preparation method thereof

The invention discloses functional gleditsia sinensis fermented royal noodles and a preparation method thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: mixing 1 / 3 of a microbial composition, gleditsia sinensis rice and water, and carrying out semi-solid fermentation to obtain a fermentation product I; and mixing the remaining 2 / 3 of the microbial composition, the fermentation product I, table salt and wheat flour, kneading into dough, and performing wire rod coiling, winding, noodle stretching and drying to obtain the functional Chinese honey locust rice fermented royal noodles. The microbial composition comprises lactobacillus reuteri, streptococcus thermophilus and saccharomycetes. The method for preparing the functional gleditsia sinensis fermented royal noodles is provided by optimizing process conditions, the cooking characteristic, the texture characteristic, the sensory characteristic and the protein in-vitro digestion characteristic of the prepared functional noodles are obviously improved, a better effect on reducing blood sugar is achieved, and good news is brought to people with high blood sugar through food therapy.
Owner:HEBEI AGRICULTURAL UNIV.

Kits and methods for super-resolution microscopy

Conventional expanded microscopy (ExM) is improved upon to show reliable and reproducible 4-fold and 12-fold modified expansion microscopy (mExM). Kits and methods are disclosed to achieve these improvements. The kits can include a sealable mold and instructions for using it in the improved methods. The kits can include a protein digestion buffer including sodium dodecyl sulfate and instructions for using the buffer in the improved methods. The improved methods can include a simplified approach to executing oxygen-sensitive reactions within the methods. The improved methods can include improved protein digestion chemistries, which can facilitate the improvements in reliability and reproducibility.
Owner:WISCONSIN ALUMNI RES FOUND

A slowly digestible quinoa flour-concentrated milk protein composite gel food and a preparation method thereof

This invention discloses a slow-digesting quinoa flour-concentrated milk protein composite gel food and its preparation method, belonging to the field of food processing technology. The invention first protects the composition as a gel food prepared from raw materials containing concentrated milk protein and quinoa flour; the preparation includes premixing quinoa flour and a portion of concentrated milk protein, hydrating them to obtain a premix with a starch-protein network, adding the remaining raw materials, stirring to form a slurry, and then shaping it. The gel food of this invention constructs a dense and continuous starch-protein network. This network, on the one hand, acts as a physical barrier to prevent effective contact between gastrointestinal digestive enzymes and internal proteins, maintaining the stability of the protein's secondary structure, thereby slowing down the rate of protein digestion and hydrolysis in the gastrointestinal tract to achieve slow digestion; on the other hand, it directly improves the product's hardness, chewiness, and other textural parameters, enhances water-holding capacity, reduces water separation rate, optimizes color and flavor, and achieves a synergistic improvement in multiple indicators of edible quality.
Owner:PEKING UNIV INST OF ADVANCED AGRI SCI +1

Low-feed coefficient grass carp biological feed and application thereof

The application belongs to the technical field of breeding feed, and specifically provides a low-feed-coefficient biological feed for grass carp, which comprises, in terms of weight fraction, 100-200 parts of pork peptides, 510-1750 parts of plant raw materials, 10-30 parts of aquatic premix, 10-30 parts of calcium dihydrogen phosphate and 1-3 parts of choline chloride. The biological feed with low feed coefficient provided by the application uses pork peptides as animal protein raw materials, which are used in combination with plant raw materials, so as to not only ensure reasonable collocation of animal and plant protein sources to balance various amino acids in the feed, but also effectively improve the digestibility of protein, thereby ensuring that the protein digestion and absorption rate is greatly improved on the premise that the formula cost does not increase greatly. The biological feed can also promote the proliferation of Cetobacterium in the intestinal tract of grass carp, thereby improving the utilization efficiency of the feed.
Owner:WUHAN SUNHY BIOLOGICAL

Tail gas absorption device of protein digestion furnace

The utility model belongs to the field of detection equipment. An exhaust port of an exhaust collector on a digestion furnace body is communicated with an exhaust absorption unit through an exhaust pipeline, the bottom of the exhaust pipeline of the exhaust absorption unit is inserted below the liquid level of a water absorption device, and the liquid level of the water absorption device is communicated below the liquid level of a sodium hydroxide solution absorption device through a gas communicating pipe. The sodium hydroxide solution absorption device is communicated with an exhaust pipe, a suction pump is arranged on the exhaust pipe, a sulfur dioxide detector and a waste discharge valve are installed at the front end and the rear end of the suction pump respectively, a bypass re-absorption pipe is arranged between the waste discharge valve and the suction pump, a gas return control valve is arranged on the re-absorption pipe, and the re-absorption pipe is communicated with a tail gas pipeline. The two-stage absorption device is arranged, the absorption effect is good, the reagent cost is low, tail gas subjected to two-stage absorption is detected through the sulfur dioxide detector, zero-pollution emission is truly achieved, the tail gas is subjected to closed continuous treatment from the exhaust gas collector to emission, the physical health of experimenters is guaranteed, and environmental pollution is reduced.
Owner:DALIAN HUAZHENG INSPECTION CO LTD

Protein detection workstation

The utility model relates to the technical field of protein detection, and provides a protein detection workstation which comprises a workbench, a digestion tube placing rack arranged on the workbench, a protein pretreatment device, a protein digestion device, a sample boat placing rack, an azotometer and a manipulator, the digestion tube placing rack, the protein pretreatment device, the protein digestion device, the sample boat placing rack and the azotometer are at least arranged on two sides of the manipulator; the manipulator can move the digestion tube from the digestion tube placing rack to the protein pretreatment device, transfer the digestion tube from the protein pretreatment device to the protein digestion device, transfer a digested protein detection solution in the digestion tube into the sample boat, and transfer the sample boat into the azotometer. According to the utility model, the digestion tube can be moved and the protein detection solution can be transferred by utilizing the manipulator according to the requirements, so that automatic operation is realized, manual operation is reduced, and the detection cost is reduced.
Owner:蒙牛乳业(宁夏)有限公司 +1

Characterization and localization of protein modifications

A method for characterizing proteins, including steps of (a) detecting a plurality of proteins, wherein individual proteins of the plurality are associated with unique identifiers, wherein the detecting distinguishes the identities of the individual proteins and the unique identifiers associated with the individual proteins; (b) digesting the proteins to form peptides, wherein the peptides from each protein are associated with the unique identifiers for the respective individual protein; (c) detecting the peptides and associated unique identifiers, wherein the detecting distinguishes characteristics of individual peptides, and wherein the detecting distinguishes unique identifiers associated with the individual peptides; and (d) correlating characteristics detected in step (c) with individual proteins detected in step (a) based on the unique identifiers associated with the individual proteins and the peptides.
Owner:NAUTILUS SUBSIDIARY INC

Active induced protein digestion efficiency optimization method and system

The invention relates to the technical field of protein digestion, in particular to an activity induced protein digestion efficiency optimization method and system. According to the method, diet data and protein data of multiple target users are collected, all data information is preprocessed to obtain preprocessed information, then an optimization model is established, representative users are screened out from the multiple target users based on the diet data through the optimization model, and user experience is improved. And respectively establishing a representative protein standard for each representative user based on the protein data of the representative user, performing multi-classification learning on the optimization model in combination with the protein standards to obtain a trained optimization model, and finally collecting the real-time diet data and the real-time protein data of the real-time user to obtain the real-time diet data and the real-time protein data of the real-time user. According to the method, the real-time protein data of the real-time user is optimized based on the real-time protein standard, the representative protein standard is established, so that the optimization model can directly adjust the digestion efficiency of the induced protein of the user based on the representative protein standard, and the adjustment efficiency is improved.
Owner:HUNAN XIAORUI AGRI ECOLOGICAL TECH CO LTD

Enzyme-immobilized particles for on-line protein digestion

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.
Owner:WATERS TECHNOLOGY CORP

A feed additive of linalool embedded microcapsule with improved taste and a preparation method thereof

The application provides a taste-improved linalool embedded microcapsule feed additive and a preparation method thereof. The additive is a microcapsule composed of core material linalool and wall material, and the wall material comprises raw material matrix, black soldier fly larvae powder, fly maggot powder, edible gelatin, edible lactose and emulsifier; the fermented raw material matrix is prepared from bean curd residue, peanut cake, wheat bran and cassava residue through co-fermentation of lactic acid bacteria and yeast. The preparation method comprises the following steps: mixing the raw material matrix and wall material components to prepare a wall material aqueous solution, adding linalool emulsion, and then performing composite coagulation, glutamine transaminase cross-linking solidification and spray drying to obtain the product. In the application, the anti-nutritional factors of raw materials are removed through fermentation, the protein digestion and utilization rate is improved, the pungent smell of linalool is shielded with the help of gelatin and lactose, the volatilization and oxidation of linalool are slowed down, the feed palatability is greatly improved, the product stability is good, and the product can be widely applied to livestock and poultry breeding feed.
Owner:NANTONG INST OF TECH

A method of preparing a marine composite prebiotic product

The application belongs to the technical field of preparation of composite prebiotic products, and particularly relates to a preparation method of a marine composite prebiotic product, which mainly comprises the following steps: (1) preparing a mixed solution of chitosan oligosaccharide and fucoidan; (2) continuously stirring the mixed solution in step (1); (3) after the mixed solution in step (2) is stirred, freeze-drying is performed to obtain a fucoidan-chitosan oligosaccharide compound; (4) preparing a fucoidan solution; (5) immersing the fucoidan-chitosan oligosaccharide compound in step (3) in the fucoidan solution in step (4); (6) continuously stirring the solution in step (5); (7) after uniform stirring, centrifugal collection is performed on the precipitate; (8) drying the precipitate; (9) repeating steps 3-8 in steps (4)-(8) for 3-8 times, and finally, the marine composite prebiotic product is obtained by drying the precipitate in the last time. The marine composite prebiotic product can effectively alleviate the inhibition of chitosan oligosaccharide on protein digestion, and has the advantages of low input cost, simple operation and short time consumption.
Owner:DALIAN POLYTECHNIC UNIVERSITY

Anti-vomiting hydrolyzed whey protein and preparation method thereof

The invention discloses anti-vomiting hydrolyzed whey protein and a preparation method thereof, and belongs to the technical field of food processing. The product provided by the invention reduces the digestion burden of protein and relieves the symptom of vomiting. According to the method disclosed by the invention, protein sequences are mutually crosslinked by virtue of a treatment mode of first fibration, second polymerization and final hydrolysis, and a hydrolyzed polypeptide sequence is changed, so that the abundance of the polypeptide SLAMAASDISLA in a hydrolysate is improved. The polypeptide has the characteristics of being easy to recognize by intestinal peptidase and relatively high in PepT1 affinity, the bioavailability of protein is improved, and the deamination proportion of unutilized amino acid is reduced, so that substances generating nitrogen-containing toxoid are reduced, the stimulation of toxin accumulation on gastrointestinal mucosa is relieved, and the symptoms of nausea, vomiting and the like of a user are avoided.
Owner:JIANGNAN UNIV

Traditional Chinese medicine composition for improving stool character

The invention relates to a traditional Chinese medicine composition for improving stool characters, and belongs to the technical field of traditional Chinese medicine preparations. The composition is prepared from potamogeton pectinatus, oriental wormwood, jerusalem artichoke, humifuse euphorbia herb, liquorice and astragalus membranaceus. In the composition, potamogeton pectinatus degrades intestinal mucoprotein MUC2 and bidirectionally adjusts the water content of excrement; artemisia capillaris inhibits putrefying bacteria proliferation, adjusts bilirubin metabolism and improves stool color. The jerusalem artichoke inhibits synthesis of hydrogen sulfide through prebiotics and short-chain fatty acid and eliminates stink; the humifuse euphorbia herb can repair intestinal mucosal barriers; licorice root anti-inflammatory film protection; astragalus membranaceus promotes protein digestion and absorption. The composition can be prepared into dosage forms such as tablets, particles and capsules, can remarkably improve viscous, thin and loose or dry and uneven states of excrement, promote discharge of formed soft excrement, relieve adhesion of excrement bodies, adjust the color of the excrement to be brownish yellow and reduce stink and exhaust, has no drug dependence risk, and is suitable for functional abnormal defecation caused by dietary imbalance of healthy people.
Owner:寇立祥

RNA co-immunoprecipitation kit for specific binding prion protein in muscle cells and RNA extraction method

The invention discloses an RNA co-immunoprecipitation kit for specific binding of prion protein in muscle cells and an RNA extraction method, and relates to the technical field of bioengineering. According to the present invention, the specific co-precipitation kit preparation and the specific extraction steps comprise cell lysis, cell lysis solution-antibody-magnetic bead incubation, protein digestion and RNA extraction, such that the high purity and the sufficient amount of the enriched RNA are successfully ensured so as to completely meet the strict requirements of the subsequent RNA-seq sequencing. The method overcomes the inherent defects of the existing RIP technology in the research of non-classical RNA binding proteins, realizes the optimization of the whole process from high-specificity enrichment to high-quality sequencing sample preparation through the integrated innovation of methodology and the kit, has the outstanding advantages of strong specificity, high sensitivity, good repeatability, simple operation and the like, and has a wide application prospect in the research of non-classical RNA binding proteins. And remarkable technical progress is achieved, and a positive application effect is generated.
Owner:SOUTHWEST JIAOTONG UNIV

Qualitative analysis of proteins

The present invention relates to a method for qualitative analysis of a sample protein, which method comprises the steps of providing a water swollen gel column bed comprising bound protease and a sample protein in liquid buffer, digesting the sample protein into polypeptides by contact with the gel bed and subjecting the polypeptides to mass spectrometry (MS). The method is advantageously performed using back and forth flow of the sample protein including two or more repeats, and may be performed at a temperature of less than about 37° C., such as a temperature of less than about 30° C.The invention also includes an automated method as well as a device and a kit for performing mass-based analysis of proteins with higher speed than the prior art while maintaining conditions that are tolerable to the protease.
Owner:PHYNEXUS INC

RNA co-immunoprecipitation-based RNA binding protein aggregate molecular function detection method

The invention relates to an RNA (Ribonucleic Acid) co-immunoprecipitation-based RNA binding protein aggregate molecular function detection method, which comprises the following steps: S1) RNA co-immunoprecipitation: firstly, splitting tissues or cells, then cleaning by using magnetic beads to remove a splitting solution, and then preparing a detection sample; s2) protein and RNA detection: firstly carrying out semi-denaturation gel electrophoresis on the sample, confirming the IP efficiency of the sample, and then recovering RNA on the membrane; s3) releasing RNA from the membrane: incubating by adopting a prepared PK buffer solution to perform protein digestion so as to extract RNA, measuring the concentration of RNA, and performing reverse transcription to obtain RNA formed by combining a DAZL polymer and a DAZL monomer; s4) performing qPCR (quantitative polymerase chain reaction) detection on a target target: performing function detection, and verifying whether RNA (ribonucleic acid) exists in the aggregate formed by combining the DAZL polymer and the monomer. The method is simple, efficient and accurate, and provides a more convenient platform for functional research of the polyamyloid protein.
Owner:NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A

High-protein coarse cereal nutritional meal replacement powder and preparation method thereof

The invention discloses high-protein coarse cereal nutritional meal replacement powder and a preparation method thereof, and relates to the field of food processing, the high-protein coarse cereal nutritional meal replacement powder comprises a continuous gel matrix phase formed through mineralization crosslinking and an adsorption slow-release phase dispersed in the continuous gel matrix phase; the continuous gel matrix phase is formed by reacting pea protein, enzymolysis oat beta-glucan and a compound mineralizing agent; the adsorption slow-release phase is composed of porous starch loaded with hydrolyzed whey protein peptide and flavor substances; the composite mineralizer comprises calcium salt and magnesium salt; according to the nutritional meal replacement powder, a three-dimensional gel network with a loose structure and strong hydrophilicity is formed through a coupling technology of constructing a continuous gel matrix phase through mineralization cross-linking and forming a dispersed slow-release phase through porous adsorption, disordered hydrophobic aggregation of protein and fibers is efficiently prevented, hydration reconstruction can be rapidly performed during brewing, and in the digestion process, the nutritional meal replacement powder has the advantages that the dissolution rate is high; the loose gel network provides a smooth permeation path for digestive enzymes, and the protein digestibility is high.
Owner:HUBEI XIANGSHANG FOOD

Application of SNP (Single Nucleotide Polymorphism) molecular marker located on pig chromosome 9 and related to nitrogen apparent digestibility

The invention discloses an SNP molecular marker located on a pig chromosome 9 and related to nitrogen apparent digestibility and application of the SNP molecular marker, the site of the SNP molecular marker is rs337541051 and corresponds to the 133972770bp site on a chromosome 9 of an international pig reference genome version 11.1, and Cgt exists at the site; t mutation. The SNP molecular marker provided by the invention is remarkably related to the nitrogen apparent digestibility character of the pig, the identification of the nitrogen apparent digestibility character of the pig can be realized by identifying the genotype of the SNP molecular marker, and the identification of the nitrogen apparent digestibility character of the pig can be realized by breeding the pig with the genotype of the SNP molecular marker CC. The nitrogen apparent digestibility of offspring can be improved, and the protein digestion and utilization capability and feed conversion efficiency of the offspring can be improved, so that the breeding process of pigs is accelerated, and genetic improvement of the pigs is realized.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Gel of lycopene and konjac glucomannan for improving protein digestion and preparation method thereof

PendingCN122460656ALycoperseneMyofibrilla
The application discloses a preparation method of a lycopene and konjac glucomannan gel for improving protein digestion, and belongs to the technical field of food processing. In view of the technical problems that the dense network of polysaccharide inhibits protein digestion and high-concentration lycopene is prone to self-agglomeration, leading to unstable antioxidant effect in the prior art, the problems are solved through the following scheme: myofibrillar proteins and konjac glucomannan are mixed in a buffer solution to perform first hydration, and a protein-polysaccharide re-dissolution is obtained; then, a heat-labile protease is added to perform second hydration, and a protein-polysaccharide-enzyme re-dissolution is obtained; lycopene is mixed with edible oil to obtain a lycopene-oil phase; the oil phase is mixed with the enzyme re-dissolution to be homogenized to prepare an oil-in-water emulsion; finally, the temperature is increased from 25 DEG C to 80 DEG C at a rate of 1-3 DEG C / min and is kept for 20-40 min, and then the temperature is cooled to 2-6 DEG C and is kept for 12-24 h to perform heat-induced gelation. The application is mainly used for preparing a gel with high gel strength, good digestion performance and antioxidant stability.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Improved library preparation for polypeptide sequencing

Provided herein are methods of preparing a polypeptide sample and methods of protein analysis. Also provided herein are compounds of Formula (I), (II), and (III), and use thereof, including in methods of protein digestion.
Owner:QUANTUM SI INC +1

A precise nutrition formula suitable for the entire physiological stage of honey possum and intestinal homeostasis and a preparation method thereof

The application discloses a kind of suitable for honey bag glider whole physiological stage intestinal homeostasis type precision nutrition formula and preparation method thereof, belong to special pet feed technical field.The formula includes basic nutrition composition 30~80 parts by weight, intestinal homeostasis composition 5~25 parts, functional additive 1~10 parts;Wherein the basic nutrition composition is with enzymolysis animal protein, insect protein, compound carbohydrate and functional oil as core, precisely match the nutritional requirement of honey bag glider whole physiological stage;Intestinal homeostasis composition adopts "microcapsule probiotic + compound prebiotic + intestinal mucosa repair factor" trinity system, and cooperatively constructs stable intestinal microecology.Preparation method adopts low-temperature conditioning granulation + probiotic post-spraying process, and guarantees the activity of functional components.The application realizes whole physiological stage free replacement grain feeding, significantly improves protein digestion rate, reduces intestinal disease incidence, with outstanding novelty, creativity and industrial practicality.
Owner:HENAN YAOKUN BIOTECHNOLOGY CO LTD

Method for improving hydrolysis degree of oxidized protein

The invention belongs to the technical field of food processing, and relates to a method for improving the hydrolysis degree of oxidized protein. According to the method, chlorogenic acid with different concentrations is used for carrying out digestion intervention on the gold pomfret myofibrillar protein oxidized by malonaldehyde, and when the concentration of the chlorogenic acid is 5-200 [mu] mol / L, the hydrolysis degree and digestibility of the gold pomfret myofibrillar protein are both improved. In addition, the chlorogenic acid can also improve the oxidation resistance of myofibrillar protein digestion products, such as ABTS < + > free radical scavenging capacity, DPPH free radical scavenging capacity and total reducing capacity. The method solves the problems of digestibility reduction and nutritional ingredient loss caused by protein oxidation in the storage and processing process of aquatic products, and provides a new way for improving the nutritional value of the aquatic products.
Owner:GUANGDONG OCEAN UNIVERSITY

Method and apparatus for evaluating the digestibility of a compressed dry ration enriched with wheat oligopeptides

The application discloses a kind of rich in wheat oligopeptide compressed dry food digestion performance evaluation method and device, the method comprises: using in vitro dynamic human gastrointestinal digestion system, not containing wheat oligopeptide compressed dry food and rich in wheat oligopeptide compressed dry food are respectively simulated digestion processing, respectively measured to obtain first measurement index set and second measurement index set;Each type of measurement index set, including gastric emptying curve, protein digestion rate sequence, amino acid composition value sequence, fat bioavailability sequence;The gastric emptying curve includes several discrete points;The first measurement index set and second measurement index set are preprocessed, and obtain preprocessed index information set;The preprocessed index information set is evaluated and processed, and obtains the digestion performance evaluation value of rich in wheat oligopeptide compressed dry food.
Owner:THE QUARTERMASTER RES INST OF THE GENERAL LOGISTICS DEPT OF THE CPLA