Feather protein peptide feed additive and application thereof

A technology of feed additive and feather protein, which is applied in application, animal feed, animal feed, etc., can solve the problems of feed protein resource shortage, restrict the sustainable development of aquaculture, and uneven quality, and achieve obvious economic and social benefits , good food-attracting effect, and the effect of improving pica

Inactive Publication Date: 2012-10-17
HANGZHOU QIMINGXING BIOLOGICAL NUTRITION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the rapid development of the global aquaculture industry characterized by intensification has made great contributions to the improvement of human living standards, but the serious shortage of feed protein resources has restricted the sustainable development of the aquaculture industry.
However, poultry feathers, which are rich in various essential amino acids of animals and have a protein content of 75% to 90%, have not been effectively utilized. In my country, 200,000 to 300,000 tons of feathers can be collected and utilized eve

Method used

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  • Feather protein peptide feed additive and application thereof
  • Feather protein peptide feed additive and application thereof
  • Feather protein peptide feed additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The kind of song preparation of embodiment 1 bacterial classification

[0026] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):

[0027] The final concentration of the slant medium is composed of: 1 g of peptone, 0.3 g of beef extract, 10.5 g of NaC, 2.0 g of agar, 100 ml of distilled water, and a pH value of 7.0.

[0028] The liquid seed medium is: glucose 0.2g, NaC 10.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH 7.0. Sterilize at 120°C for 30 minutes.

[0029] Inoculate Bacillus subtilis ACCC01185 on the slant medium, and culture it at 37°C for 24 hours to obtain the slant strain; pick an inoculation loop from the slant strain and put it in a 300ml Erlenmeyer flask containing 50ml sterile liquid seed medium, and place it at 37°C. Cultivate on a warm shaker at a speed of 220r / min, and stop when the spore rate reaches more than 90% (about 24 hours), and obtain the s...

Embodiment 2

[0038] Embodiment 2 Feather protein peptide feed additive

[0039] (1) Preparation of yeast culture:

[0040] The preparation method of the yeast culture: put the fermentation substrate composed of 600g of bran, 30g of corn flour, 150g of distiller's grains, 200g of soybean meal powder, and 20g of urea into a sterilized tank, then add 650g of water, and cook for 35min at a pressure of 0.12Mpa Sterilize, close the steam inlet valve after sterilization, and discharge the material for 35 minutes to obtain a solid-state fermentation medium. Ventilate and cool to 33-35°C, respectively inoculate 40 g of Saccharomyces cerevisiae ACCC20042 bacteria-containing cell suspension prepared by the method in Example 1 (the number of viable bacteria reaches 1.2×10 in terms of dry weight of solid-state fermentation medium) 7 cfu / g), 50g of cell suspension containing bacteria in ACCC20015 (the number of viable bacteria is 2×10 based on the dry weight of the solid-state fermentation medium) 7 c...

Embodiment 3

[0043] Embodiment 3 Feather protein peptide feed additive

[0044] (1) Yeast culture preparation

[0045] The preparation method of the yeast culture: put the fermentation substrate composed of 400g of bran, 100g of corn flour, 200g of distiller's grains, 250g of soybean meal powder, and 50g of urea into a sterilized tank, add 1000g of water, and cook for 35min at a pressure of 0.12Mpa. Sterilize, close the steam inlet valve after sterilization, and discharge the material for 35 minutes to obtain a solid-state fermentation medium. Ventilate and cool to 33~35°C, respectively inoculate 120 g of Saccharomyces cerevisiae ACCC20042 bacteria-containing cell suspension prepared by the method in Example 1 (the number of viable bacteria reaches 3.6×10 in terms of dry weight of solid-state fermentation medium) 7 cfu / g), 125g of bacterial cell suspension containing Saccharomyces fumelliformis ACCC20015 (the number of live bacteria is 5×10 based on the dry weight of the solid-state ferme...

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Abstract

The present invention discloses a feather protein peptide feed additive and an application thereof. The preparation method for the feather protein peptide feed additive comprises: 1, mixing a substrate and water to obtain a solid state fermentation culture medium A, inoculating saccharomyces cerevisiae and saccharomycopsis fibuligera in the solid state fermentation culture medium A, culturing for 28-30 hours at a temperature of 30 DEG C, and drying the resulting culture at a temperature of 35-45 DEG C after culturing to obtain a yeast culture; and 2, mixing avian feather powder and the yeast culture, adding water to the resulting mixed material, uniformly stirring to obtain a solid state fermentation culture medium B, respectively inoculating bnfillus licheniformis and bacillus subtilis in the solid state fermentation culture medium B, and carrying out fermentation culture for 30-45 hours at a temperature of 35-38 DEG C to obtain the feather protein peptide feed additive. The feather protein peptide feed additive of the present invention has the following advantages that: feather protein digestion absorption efficiency can be 90%; the additive contains a variety of bioactive peptides, and provides digestion and growth promotion effects; the additive has natural fermentation flavor and good palatability; and pica of livestock and poultry can be significantly improved.

Description

(1) Technical field [0001] The invention relates to a feather protein peptide feed additive and its preparation and application. (2) Background technology [0002] In recent years, the rapid development of the global aquaculture industry characterized by intensification has made a great contribution to the improvement of human living standards, but the serious shortage of feed protein resources has restricted the sustainable development of the aquaculture industry. However, poultry feathers, which are rich in various essential amino acids of animals and have a protein content of 75% to 90%, have not been effectively utilized. In my country, 200,000 to 300,000 tons of feathers can be collected and utilized every year, and less than 20,000 of them are used for feed after processing. Tons, most of the feathers and animal hairs have not been rationally utilized, which not only wastes resources, but also pollutes the environment. The main reason why feathers cannot be used is tha...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16
Inventor 徐张贤金妙仁陈安国洪奇华王萍
Owner HANGZHOU QIMINGXING BIOLOGICAL NUTRITION
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