Elastin digest compositions and methods utilizing same
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example 1
[0093]An elastin digest may be any elastin peptide mixture derived from mammalian tissue. For example, the following steps may be used to prepare an elastin digest. Specifically, ProK formulations manufactured by Human Matrix Sciences, is an elastin peptide mixture that may be prepared as follows. Bovine neck ligaments are obtained from local meat packer and obtain USDA certification on the geographic origin of donor cattle. All or most of fat and extraneous connective tissue is removed from dissected bovine neck ligaments. The neck ligaments are ground into small pieces by first running through coarse grind then fine grind modes on an industrial meat grinder. The ground neck ligaments are then suspended in aqueous 1% NaCl at a ratio of 100 gm tissue to 3 liter 1% NaCl. The mixture is stirred for 24 hours at 4° C. to extract soluble connective tissue proteins from ground neck ligaments. The extract from ground ligaments is removed by pouring mixture through a fine mesh kitchen strai...
example 2
[0100]The following example details a formulation of a composition of the present invention and a method for making such a composition using an already hydrolyzed form of elastin, such as Elastin E91 or ProK. In Phase A, where the ingredient (% w / w) is purified water the quantity is QSAD 100.00. Where the ingredient is Glycerin 99.50% , the range is 5.0 to 15.0 (% w / w), preferably 10.0 (% w / w). Where the ingredient is Methyparaben, the range is 0.004 to 0.40 (% w / w) from Ueno, preferably 0.20 (% w / w). Where the ingredient is Ethylparaben, the range is 0.002 to 0.20 (% w / w) from Ueno, preferably 0.10 (% w / w). In Phase C, where the ingredient is Butylparaben, the range is 0.002 to 0.20 (% w / w) from Ueno, preferably 0.10 (% w / w). Where the ingredient is Propylparaben, the range is 0.001 to 0.20 (% w / w) from Ueno, preferably 0.10 (% w / w). Where the ingredient is Squalane, the range is 0.05 to 10.00 (% w / w) from Robeco, preferably 5.00 (% w / w). Where the ingredient is Caprylic / Capric Tri...
example 3
[0102]The following is a prophetic example illustrating the preparation and administration of a therapeutic composition according to one embodiment of the present invention. Insoluble Elastin from bovine neck ligament is prepared and digested with proteinase K using the materials and methods described in Example 1. A sample of the filtrate from the digestion of the insoluble bovine Elastin with proteinase K was analyzed via LC / MS / MS and the resultant chromatograph peaks identifying the peptides. A possible cleavage of ELS_BOVIN (P04985) from SWISS-PROT and cleaved with Proteinase K using the PeptideCutter program from the ExPASy web site on the world wide web URL address us.expasy.org, gives the peptides and cleavages sites corresponding to SEQ ID NO: 23-44 in Table 1 and di-peptides listed in Table 2.
[0103]The remaining filtrate was purified by affinity chromatography with a receptor protein bound to the chromatographic support to isolate the peptide of interest (e.g. SEQ ID NO: 44...
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