The invention discloses a test tube bulblet reproduction method of lilium leucanthum, belonging to the field of biology. The invention aims to provide the test tube bulblet reproduction method of lilium leucanthum, so as to solve the problem that existing common methods for lily tissue cultivation cause slow growing of seedlings of lilium leucanthum. The method comprises steps of sterilizing of scale explants, inducing the test tube bulblet, expanding and root growing of the test tube bulblet, and germinating and growing of the bulblet. A bulblet induction culture medium comprises a base culture medium and hormones, wherein the base culture medium is prepared from a MS base culture solution, 60g / L of sucrose, 0.5g / L of arginine phosphokinase, 0.5g / L of glutamine, 2g / L of active carbon, 6g / L of agar, 500mg / L of dithiothreitol and 300-800mg / L of butylparaben. The test tube bulblet reproduction method of lilium leucanthum has the advantages of high reproduction coefficient, short cultivation cycle, high efficiency, high transplant survival rate, convenient transportation, easy operation and low cost.