The invention discloses a degradable slow-release invisible eye mask capable of eliminating eye fatigue and a preparation method of the degradable slow-release invisible eye mask, and relates to the technical field of invisible eye masks. Per each 10ml, the invisible eye mask comprises the following raw material components: 200mg-300mg of collagen peptide, 0.8-1.2mg of hyaluronic acid, 4-6mg of vitamin B6, 1.8-2.2mg of vitamin B12, 9-11 mg of dipotassium glycyrrhizinate, 0.18-0.22mg of napthoxylin hydrochloride, 0.28-0.32mg of neostigmine methylsulfate, 0.8-1.2mg of chlorpheniramine maleate, 80-150mg of glycerinum, 7-9mg of dexpanthenol, 4-6mg of menthol, 5-7mg of polysorbate 80, 5-7mg of borneol, 3-5mg of edetate disodium, 2-4mg of mint, 2-4mg of aminocaproic acid, 1-3mg of ethanol, 9-11mg of sodium chloride, 2-4 mg of benzalkonium chloride solution, and 3-5 mg of chlorobutanol solution; the mass fraction of the benzalkonium chloride solution is 0.01%; and the mass fraction of the chlorobutanol solution is 0.2%.