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Test tube bulblet reproduction method of lilium leucanthum

A technology for small bulbs and lilies is applied in the field of cultivating small bulbs of purple ridge lily in test tubes, which can solve problems such as slow growth of seedlings, and achieve the effects of reducing nutrient consumption, inhibiting the occurrence of polycephaly, and inhibiting browning.

Active Publication Date: 2018-02-27
甘肃绿能农业科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the propagation of small bulbs of lily purple ridge in test tubes, so as to solve the problem of slow growth of seedlings caused by the method commonly used in the existing lily tissue culture for the propagation of lily purple ridge

Method used

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  • Test tube bulblet reproduction method of lilium leucanthum
  • Test tube bulblet reproduction method of lilium leucanthum
  • Test tube bulblet reproduction method of lilium leucanthum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1- Embodiment 12

[0035] Example 1-Example 12: Induction of bulblets

[0036] 1. Disinfection of scale explants: remove the outermost scales with lesion spots on the outermost layer of lily lily, break off the outer and middle scales along the bulb plate, rinse them with running water, soak them in 0.2% mercuric chloride for 15-20 minutes, and then extinguish them. Rinse with sterilized distilled water, cut off 1 / 3 of the tip of the scales, and cut the remaining scales longitudinally into 1.5-2 cm strips for later use.

[0037] 2. Induction of test tube bulblets

[0038]The above-mentioned scales are used as explants, and inoculated in the bulblet induction medium at a temperature of 25-27°C under dark conditions. The bulblet induction medium includes a basal medium and different hormones are added for cultivation. The basal medium consists of : MS basic culture medium, sucrose 60g / L, arginine 0.5g / L, glutamine 0.5g / L, activated carbon 2g / L, agar 6g / L, dithiothreitol 500 mg / L, paraben Butyl e...

Embodiment 13- Embodiment 22

[0050] Example 13-Example 22: Swelling and rooting of tubelet bulblets

[0051] Transfer the scales with small bulbs into the small bulb expansion and rooting medium, and culture them in dark under the condition of changing temperature at 22°C for 10 hours / 16°C for 14 hours every day. After 20 days, separate the small bulbs from the scales and transfer them to the same culture medium basal, and continued to culture for 60 days under the same conditions. At this time, the root length of the bulblets in the test tube is about 1-3 cm, and the diameter is about 1-1.3 cm. Carefully harvest the bulblets for substrate transplantation.

[0052] The test tube bulblet expansion and rooting medium is composed of basic medium with different combinations and concentrations of hormones. The composition of the basic medium is: MS basic medium, sucrose 60g / L, acid hydrolyzed casein 1g / L, arginine 0.5 g / L, glutamine 0.5g / L, activated carbon 2g / L, agar 6g / L, pH 6.0. The ratio of different hor...

Embodiment 23- Embodiment 26

[0057] Embodiment 23-Embodiment 26: Germination growth of small bulbs

[0058] Take out the rooting bulblets from the culture medium when the roots are 2 cm long, rinse them with running water, soak them in 3-10 mg / L karrikinolide solution, see Table 4 for the soaking concentration, soak for 60 minutes, and sow them in the medium with a volume ratio of 1:1. In the mixed matrix of vermiculite and perlite, it grows under natural conditions. Water 1g / L water-soluble fertilizer once every 7 days, and the type of water-soluble fertilizer is (15-15-30+TE). Leaves grow about 20 days after sowing, and after the seedlings fall naturally, the bulbs are harvested for field cultivation in the next year.

[0059] "Small bulb germination growth test"

[0060] See Table 4 for the germination acceleration treatment and results of the small bulbs. In Comparative Example 7, no karrikinolide solution was added, and other conditions were the same as in Example 23.

[0061]

[0062] It can b...

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Abstract

The invention discloses a test tube bulblet reproduction method of lilium leucanthum, belonging to the field of biology. The invention aims to provide the test tube bulblet reproduction method of lilium leucanthum, so as to solve the problem that existing common methods for lily tissue cultivation cause slow growing of seedlings of lilium leucanthum. The method comprises steps of sterilizing of scale explants, inducing the test tube bulblet, expanding and root growing of the test tube bulblet, and germinating and growing of the bulblet. A bulblet induction culture medium comprises a base culture medium and hormones, wherein the base culture medium is prepared from a MS base culture solution, 60g / L of sucrose, 0.5g / L of arginine phosphokinase, 0.5g / L of glutamine, 2g / L of active carbon, 6g / L of agar, 500mg / L of dithiothreitol and 300-800mg / L of butylparaben. The test tube bulblet reproduction method of lilium leucanthum has the advantages of high reproduction coefficient, short cultivation cycle, high efficiency, high transplant survival rate, convenient transportation, easy operation and low cost.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for cultivating test tube bulblets of lily ridge. Background technique [0002] Yichang lily is a unique species in China, mainly distributed in Hubei and Sichuan. The flowers are trumpet-shaped and slightly fragrant. It can not only extract essential oils, but also has high ornamental value. It can be used in the landscaping industry. Its bulbs have analgesic, anti-inflammatory and detoxification , antibacterial, anti-oxidation and other effects, can be used in the development of functional food and medicine. The wild resources of Purple Ridge Lily are mainly distributed along the Qinba Mountains in Longnan and Gannan, Gansu Province; Purple Ridge Lilium is a variety of Yichang Lilium, which is similar to Yichang Lily in function and use, and the difference from Yichang Lilium in shape lies in the perianth There are purple or brown ridges on the outside of the sheet,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨涛朱瑞清李鑫郑群杨晖王治业
Owner 甘肃绿能农业科技股份有限公司
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