7results about How to "Promote rooting" patented technology

The invention provides a method for promoting aeroponic rooting of pinus khasys, and relates to the technical field of cutting propagation of pinus khasys. The method for aeroponically culturing and rooting the pinus khasys comprises the following steps: soaking scions of the pinus khasys in a citric acid solution in an ice-water bath, disinfecting, treating with a rooting agent, and aeroponically culturing by adopting a nutritional agent added with calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indolebutyric acid, sodium phytate and lauryl alcohol polyether-4. According to the method, the defects in the prior art are overcome, rapid rooting of the pinus kesiya branches is effectively promoted, growth of the root system is guaranteed, a rapid and convenient method is provided for stable seedling raising of the pinus kesiya, and artificial breeding of the pinus kesiya is guaranteed.

The application belongs to the technical field of plant cultivation and particularly relates to a Blumea riparia growth medium, a tissue culture medium, a tissue culture method and application. The Blumea riparia growth medium takes MS medium as a basic medium and further comprises 0.2-0.3 mg / L NAA, 0.08-0.16 mg / L DA-6, 10 mg / L vitamin C, 20 mg / L myo-inositol, 30 g / L sucrose and 6 g / L agar. DA-6 and NAA are jointly applied to improve the rooting efficiency, root length and plant height of Blumea riparia. The Blumea riparia germplasm with high content of levorotatory borneol is used as an explant to carry out tissue culture and seedling raising, the Blumea riparia tissue culture seedling has high content of levorotatory borneol, and Blumea riparia stable genetic good seedlings can be produced.

The application provides a sugarcane tissue culture method, and belongs to the technical field of sugarcane tissue culture, and the tissue culture method comprises the following steps: (1) taking sugarcane tender stems as explants, alcohol disinfection, and obtaining pretreated explants; (2) placing the pretreated explants in an induction culture medium, inducing culture, and obtaining callus; (3) placing the callus in a proliferation culture medium, proliferating culture, and obtaining adventitious buds; (4) placing the adventitious buds in a rooting culture medium, rooting culture, and obtaining tissue culture seedlings; (5) placing the tissue culture seedlings in a seedling raising substrate, seedling raising, and obtaining seedlings; the seedling raising substrate in step (5) comprises the following raw material components in mass parts: 10-20 parts of sugarcane residues, 40-60 parts of peat soil, 10-15 parts of sheep manure, and 8-10 parts of rice husk charcoal. The tissue culture method provided by the application can improve the rooting rate and transplanting survival rate of sugarcane tissue culture seedlings, and the growth traits of the tissue culture seedlings can also be significantly improved.

This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.

The utility model belongs to water conservancy slope protection technical field especially relates to a water conservancy slope protection. The utility model provides a water conservancy slope protection, including the slope protection, still include: protection device, protection device fixed setting on the slope protection, the slope protection device is used to fix the silt on the slope protection, the protection device includes the planting interval, the planting interval is used to provide the space of plant growth. In this technical scheme, the planting interval on the protection device can provide the space of plant growth, so that the plant can better survive on the slope protection, so that the soil can be better fixed on the slope protection, not only is favorable to the ecological environment friendly, also prevents the collapse of the slope protection caused by the water and soil loss.

This invention discloses a method for efficient shrub planting in photovoltaic bases in high-altitude Gobi desert areas, belonging to the field of shrub planting technology. The method includes: improving the soil in the planting area to construct a root zone environment suitable for the colonization of cold-resistant mycorrhizal fungi; establishing mycorrhizal induction conditions through bio-fermentation to form a mycelial network; adding a mineral water-retaining matrix to the root zone and applying low-frequency micro-vibration to promote mineral particle rearrangement and form a continuous capillary moisture-conducting network; setting up a deep moisture-conducting structure and an upper and lower heat-insulating structure in the root zone, and combining drip irrigation for water and fertilizer management to achieve the synergistic construction of the micro-ecological environment in the shrub root zone. This invention improves the rapid colonization capacity of mycorrhizae and the continuous transport capacity of deep water through the synergistic regulation of the mycorrhizal network, capillary moisture-conducting network, and root zone thermal environment, enhances the root system's absorption efficiency of water and nutrients, improves the temperature and humidity environment in the root zone, increases the survival rate of shrubs, shortens the seedling establishment period, and improves the vegetation restoration efficiency of photovoltaic bases in high-altitude Gobi desert areas.

This invention discloses a method for tissue culture of *Anoectochilus pingbianense*, comprising: selecting wild *Anoectochilus pingbianense*, removing leaves and roots, rinsing, cutting stem segments, soaking in alcohol and rinsing with sterile water, soaking in sodium hypochlorite and rinsing with sterile water again to obtain treated stem segments; inoculating the treated stem segments into an induction medium for culture; inoculating the obtained stem segments into a rooting medium for culture; and inoculating the obtained stem segments into a tissue culture seedling medium for culture to obtain tissue culture seedlings with complete roots, stems and leaves. This invention solves the problems of severe browning and long propagation cycle in existing *Anoectochilus pingbianense* tissue culture, which cannot meet production needs, greatly reduces possible contamination during tissue culture, reduces the browning rate of explants, and shortens the growth cycle of tissue culture seedlings.