Wetland ecological restoration technology

Abstract

The invention relates to the technical field of ecological restoration, and particularly discloses a wetland ecological restoration technology. The wetland ecological restoration technology comprises the following steps: step 1) mixing bradyrhizobium angularis, flavobacterium, pseudomonas saccharophila, a nutrient substrate and water, and culturing for 7-9 days to obtain a culture solution; the method comprises the following steps of 1, preparing a culture solution, 2, cutting reed rhizomes into segments, and soaking the segments in the culture solution for 1-2 days to obtain pretreated rhizomes, and 3, inserting the pretreated rhizomes into sewage-covered soil for planting until the reeds grow to purify the sewage. The method has the advantages that the survival rate of the reeds in the sewage is increased, and wetland ecological restoration is accelerated.

Description

Technical Field

[0001] The invention relates to the field of ecological restoration, and in particular to a wetland ecological restoration technology. Background Art

[0002] Wetland ecosystems belong to aquatic ecosystems. Their biological communities are composed of aquatic and terrestrial species, with active material circulation, energy flow, species migration and evolution, and high ecological diversity, species diversity and biological productivity.

[0003] With the intensification of human activities, some wetland ecosystems have been destroyed. Water pollution in wetlands is one of the main causes of wetland ecological destruction. Water pollution is mainly caused by high COD in the water, which makes it difficult for organisms to survive. Due to the large area of ​​wetlands, it is difficult to use sewage treatment equipment to purify wetland water. Therefore, it is necessary to improve the wetland ecology by planting plants with water purification functions.

[0004] Existing technology shows that reeds have good removal effects on ammonia nitrogen and COD in wastewater. Reed planting methods are divided into rhizome planting and sowing planting. Due to the low survival rate of sowing planting, rhizome planting is usually used. Although under normal circumstances, the survival rate of rhizome planting is high, it is found in practice that planting reeds directly in high-COD sewage by rhizome planting will lead to a significant decrease in the survival rate. Rhizome planting has the defect of high labor intensity. The significant decrease in survival rate will require multiple sowing, resulting in a more significant increase in labor intensity. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to improve the survival rate of reeds in sewage and accelerate wetland ecological restoration, the present application provides a wetland ecological restoration technology.

[0006] The wetland ecological restoration technology provided in this application adopts the following technical solution: A wetland ecological restoration technology comprises the following steps: Step 1), mixing Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophilus, a nutrient substrate and water, and culturing for 7-9 days to obtain a culture solution; Step 2), cutting the reed rhizomes into segments and soaking them in a culture solution for 1-2 days to obtain pretreated rhizomes; Step 3), insert the pre-treated rhizomes into the soil covered with sewage for planting, and wait for the reeds to grow to purify the sewage.

[0007] By adopting the above technical scheme, by planting reeds, the pollutants in sewage can be absorbed by the reeds, thereby purifying the water body and further realizing the effect of wetland ecological restoration. By adopting the culture solution composed of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophilus, nutrient substrate and water, the reed rhizomes can be attached with a bacterial film when immersed in the culture solution. The special bacterial film and its secretion products can significantly promote the rooting of the reeds, so that the reeds can have a higher survival rate in severely polluted sewage, reduce repeated planting, and thus reduce labor intensity.

[0008] Preferably, the mass ratio of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water is 0.01-0.02:0.03-0.04:0.02-0.03:150-160:1000.

[0009] By adopting the above technical scheme and specifically selecting the mass ratio of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water, the effect of Bradyrhizobium elsdenii, Flavobacterium and Pseudomonas saccharophila cooperating with each other is better, a special bacterial film is better formed on the rhizomes of reeds, the rooting of reeds is better promoted, and the survival rate of reeds is higher.

[0010] Preferably, the nutrient substrate is a compound of glucose, peptone, sodium chloride, green plum juice and roxburghii juice.

[0011] By adopting the above technical scheme, by specifically selecting the nutrient substrate as a combination of glucose, peptone, sodium chloride, green plum juice and sea buckthorn juice, the activity of Bradyrhizobium elsdenii, Flavobacterium and Pseudomonas saccharophila can be effectively improved, the efficiency of culturing Bradyrhizobium elsdenii, Flavobacterium and Pseudomonas saccharophila is higher, the preparation time of the nutrient solution is shortened, and the efficiency is higher.

[0012] Preferably, the mass ratio of the glucose, peptone, sodium chloride, green plum juice and rosa roxburghii juice is 40-42:35-37:15-17:33-35:27-29.

[0013] By adopting the above technical scheme and specifically selecting the mass ratio of glucose, peptone, sodium chloride, green plum juice and sea buckthorn juice, the activity of Bradyrhizobium elsdenii, Flavobacterium and Pseudomonas saccharophila can be further improved, so that the nutrient solution can better promote the rooting of reeds and increase the survival rate of reeds.

[0014] Preferably, in the step 1), Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water are mixed and cultured at a constant temperature of 36-38° C. for 7-9 days.

[0015] By adopting the above technical solution and maintaining a constant temperature of 36-38°C, the activities of Bradyrhizobium elsdenii, Flavobacterium saccharophilum, and Pseudomonas saccharophila are higher, and they can reproduce more quickly, and the efficiency of forming the culture solution is higher.

[0016] Preferably, in step 2), the reed rhizomes are cut into segments and then immersed in a culture solution at a constant temperature of 36-38° C. for 1-2 days.

[0017] By adopting the above technical solution and maintaining a constant temperature of 36-38°C, the reed rhizomes can be covered with the biofilm faster and more efficiently.

[0018] Preferably, in step 2), the length of the reed rhizomes after being cut into segments is 10-12 cm.

[0019] By adopting the above technical solution and specifically selecting the length of the reed rhizomes after cutting into segments of 10-12 cm, the survival rate of the reeds can be further improved.

[0020] Preferably, in step 3), the water depth of the soil covered with sewage for inserting the pretreated rhizomes is 0.3-0.6 m.

[0021] By adopting the above technical solution and specifically selecting a water depth of 0.3-0.6 m, the survival rate of reeds is high and the effect of purifying sewage is better.

[0022] Preferably, in step 3), the depth of the pretreated rhizomes inserted into the soil is 5-8 cm.

[0023] By adopting the above technical solution and specifically selecting to insert 5-8cm, it helps to improve the survival rate of reeds and reduce repeated planting.

[0024] In summary, this application has the following beneficial effects: 1. Since the present application can utilize reeds to absorb pollutants in sewage through planting reeds, the water body can be purified, thereby achieving the effect of wetland ecological restoration. The culture solution composed of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophilus, nutrient substrates, and water can make the rhizomes of reeds attached with bacterial films when they are immersed in the culture solution. The special bacterial films and their secretion products can significantly promote the rooting of reeds, so that reeds can have a higher survival rate in severely polluted sewage, reduce repeated planting, and thus reduce labor intensity.

[0025] 2. In the present application, it is preferred to specifically select the mass ratio of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water so that the cooperation between Bradyrhizobium elsdenii, Flavobacterium and Pseudomonas saccharophila is better, a special biofilm is better formed on the rhizomes of reeds, reed rooting is better promoted, and the survival rate of reeds is higher.

[0026] 3. In the present application, it is preferred that the nutrient substrates are specifically selected to be a combination of glucose, peptone, sodium chloride, green plum juice and sea buckthorn juice, which can effectively improve the activity of Bradyrhizobium elsdenii, Flavobacterium saccharophila and Pseudomonas saccharophila, and the efficiency of culturing Bradyrhizobium elsdenii, Flavobacterium saccharophila and Pseudomonas saccharophila is higher, thereby shortening the preparation time of the nutrient solution and increasing the efficiency. DETAILED DESCRIPTION

[0027] The present application is further described in detail below with reference to the embodiments.

[0028] Example 1 A wetland ecological restoration technology comprises the following steps: Step 1), 0.01 kg of Bradyrhizobium elsdenii, 0.03 kg of Flavobacterium, 0.02 kg of Pseudomonas saccharophila, 40 kg of glucose, 35 kg of peptone, 15 kg of sodium chloride, 33 kg of green plum juice, 27 kg of roxburghii juice, and 1000 kg of water are mixed, and cultured at a constant temperature of 36° C. for 7 days to obtain a culture solution.

[0029] Step 2), cutting the reed rhizomes into segments with a length of 10 cm, leaving one node in each rhizome segment, and then soaking the rhizome segments in a culture solution at a constant temperature of 36° C. for 1 day to obtain pretreated rhizomes.

[0030] Step 3), select a soil area covered with sewage with a water depth of 0.3m, vertically insert the pretreated rhizomes into the soil for planting, the depth of the pretreated rhizomes inserted into the soil is 5cm, and wait for the reeds to grow to purify the sewage.

[0031] Bradyrhizobium elsdenii was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D18261.

[0032] Flavobacterium was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D50040.

[0033] Pseudomonas saccharophila was purchased from Beijing Biobio Biotech Co., Ltd. with the number: Bio-15346.

[0034] Glucose was purchased from Zhitian Yuzhou Chemical Plant in Gongyi City, CAS No.: 89798-27-6.

[0035] Peptone was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 73049-73-7.

[0036] Sodium chloride was purchased from Shandong Poly Chemical Co., Ltd., CAS No.: 7647-14-5.

[0037] Green plum juice was purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0038] The sea buckthorn juice was purchased from Henan Baizhitang Pharmaceutical Group Co., Ltd.

[0039] Example 2 A wetland ecological restoration technology comprises the following steps: Step 1), 0.015kg of Bradyrhizobium elsdenii, 0.035kg of Flavobacterium, 0.02-0.03kg of Pseudomonas saccharophila, 41kg of glucose, 36kg of peptone, 16kg of sodium chloride, 34kg of green plum juice, 28kg of roxburghii juice, and 1000kg of water are mixed, and cultured at a constant temperature of 37° C. for 8 days to obtain a culture solution.

[0040] Step 2), cutting the reed rhizomes into segments with a length of 11 cm, leaving one node in each rhizome segment, and then soaking the rhizome segments in a culture solution at a constant temperature of 37° C. for 1 day to obtain pretreated rhizomes.

[0041] Step 3), select a soil area covered with sewage with a water depth of 0.5m, vertically insert the pretreated rhizomes into the soil for planting, the depth of the pretreated rhizomes inserted into the soil is 6cm, and wait for the reeds to grow to purify the sewage.

[0042] Bradyrhizobium elsdenii was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D18261.

[0043] Flavobacterium was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D50040.

[0044] Pseudomonas saccharophila was purchased from Beijing Biobio Biotech Co., Ltd. with the number: Bio-15346.

[0045] Glucose was purchased from Zhitian Yuzhou Chemical Plant in Gongyi City, CAS No.: 89798-27-6.

[0046] Peptone was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 73049-73-7.

[0047] Sodium chloride was purchased from Shandong Poly Chemical Co., Ltd., CAS No.: 7647-14-5.

[0048] Green plum juice was purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0049] The sea buckthorn juice was purchased from Henan Baizhitang Pharmaceutical Group Co., Ltd.

[0050] Example 3 A wetland ecological restoration technology comprises the following steps: Step 1), 0.02kg of Bradyrhizobium elsdenii, 0.04kg of Flavobacterium, 0.03kg of Pseudomonas saccharophila, 42kg of glucose, 37kg of peptone, 17kg of sodium chloride, 35kg of green plum juice, 27-29kg of roxburghii juice, and 1000kg of water are mixed, and cultured at a constant temperature of 38° C. for 9 days to obtain a culture solution.

[0051] Step 2), cutting the reed rhizomes into segments with a length of 12 cm, leaving one node in each rhizome segment, and then soaking the rhizome segments in a culture solution at a constant temperature of 38° C. for 2 days to obtain pretreated rhizomes.

[0052] Step 3), select a soil area covered with sewage with a water depth of 0.6m, vertically insert the pretreated rhizomes into the soil for planting, the depth of the pretreated rhizomes inserted into the soil is 8cm, and wait for the reeds to grow to purify the sewage.

[0053] Bradyrhizobium elsdenii was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D18261.

[0054] Flavobacterium was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D50040.

[0055] Pseudomonas saccharophila was purchased from Beijing Biobio Biotech Co., Ltd. with the number: Bio-15346.

[0056] Glucose was purchased from Zhitian Yuzhou Chemical Plant in Gongyi City, CAS No.: 89798-27-6.

[0057] Peptone was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 73049-73-7.

[0058] Sodium chloride was purchased from Shandong Poly Chemical Co., Ltd., CAS No.: 7647-14-5.

[0059] Green plum juice was purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0060] The sea buckthorn juice was purchased from Henan Baizhitang Pharmaceutical Group Co., Ltd.

[0061] Comparative Example 1 A wetland ecological restoration technology, compared with Example 2, the only difference is: Bacillus megaterium was used to replace Bradyrhizobium elsdenii in equal amounts.

[0062] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, with the strain number: SHBCC D50759.

[0063] Comparative Example 2 A wetland ecological restoration technology, compared with Example 2, the only difference is: An equal amount of Paenibacillus jelly was used to replace Flavobacterium.

[0064] The jelly-like Bacillus was purchased from Shanghai Collection Biotechnology Center, with the strain number: SHBCC D53180.

[0065] Comparative Example 3 A wetland ecological restoration technology, compared with Example 2, the only difference is: Paecilomyces lilacinus was used to replace Pseudomonas saccharophila in equal amounts.

[0066] Paecilomyces lilacinus was purchased from Shanghai Collection Biotechnology Center, with the strain number: SHBCC D22618.

[0067] Comparative Example 4 A wetland ecological restoration technology, compared with Example 2, the only difference is: Bacillus megaterium was used to replace Bradyrhizobium elsdenii in equal amounts, Paenibacillus gelatinosarum was used to replace Flavobacterium in equal amounts, and Paecilomyces lilacinus was used to replace Pseudomonas saccharophila in equal amounts.

[0068] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, with the strain number: SHBCC D50759.

[0069] The jelly-like Bacillus was purchased from Shanghai Collection Biotechnology Center, with the strain number: SHBCC D53180.

[0070] Paecilomyces lilacinus was purchased from Shanghai Collection Biotechnology Center, with the strain number: SHBCC D22618.

[0071] Comparative Example 5 A wetland ecological restoration technology, compared with Example 2, the only difference is: Replace the plum juice with an equal amount of apple juice.

[0072] The apple juice was purchased from Hebei Lvnuo Food Co., Ltd.

[0073] Comparative Example 6 A wetland ecological restoration technology, compared with Example 2, the only difference is: Replace the sea buckthorn juice with an equal amount of snow pear juice.

[0074] Sydney Juice purchased Ningxia Vanilla Biotechnology Co., Ltd.

[0075] Comparative Example 7 A wetland ecological restoration technology, compared with Example 2, the only difference is: Replace the green plum juice with an equal amount of apple juice, and replace the sea buckthorn juice with an equal amount of pear juice.

[0076] The apple juice was purchased from Hebei Lvnuo Food Co., Ltd.

[0077] Sydney Juice purchased Ningxia Vanilla Biotechnology Co., Ltd.

[0078] Experiment 1 The survival rate experiment was carried out using the pretreated rhizomes obtained in step 2 of each embodiment and comparative example.

[0079] A polluted wetland was selected, and the water depth was measured to find a range of 0.3-0.4 m. The pretreated rhizomes were planted in groups, with the pretreated rhizomes of each embodiment or comparative example forming a group, and a group containing 1000 pretreated rhizomes.

[0080] During planting, the pretreated rhizomes were vertically inserted into the soil with a depth of 5 cm. The interval between each plant was 30 cm. Each group of pretreated rhizomes was planted together with an interval of 5 m between groups to form multiple test areas.

[0081] After planting, no further care was done and the plants were allowed to grow freely. After 30 days, the number of root and stem sprouts in each test area was observed and the survival rate was calculated according to the following formula: Survival rate (%) = number of germinated rhizomes ÷ 1000 × 100 The above experiment was repeated ten times, and the final survival rate was taken as the average.

[0082] The water quality was tested ten times randomly in the experimental area, and the average value of chemical oxygen demand (COD) in the sewage was recorded to be 382.24 mg / L.

[0083] The specific experimental results of Experiment 1 are shown in Table 1.

[0084] Table 1 According to the data comparison of each embodiment and the comparative example in Table 1, it can be obtained that the survival rate of each embodiment is significantly higher than that of each comparative example. It can be seen that when the rhizomes of reeds are soaked in a nutrient solution composed of Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, glucose, peptone, sodium chloride, green plum juice and sea buckthorn juice, the rooting of reeds can be promoted and the survival rate of reeds can be significantly improved. Although comparative examples 1-4 use other microorganisms with rooting effects to replace Bradyrhizobium elsdenii, Flavobacterium, and Pseudomonas saccharophila in the embodiments, although they can also play a role in promoting rooting, the effect of promoting reed rooting is relatively general, especially in sewage with a COD of 382.24 mg / L, it is difficult to significantly improve the survival rate of reeds.

[0085] Comparative Examples 5-7 use other fruit juices to replace the green plum juice and sea buckthorn juice in the examples alone or in whole, resulting in changes in the color of the nutrients in the nutrient solution, and a decrease in the effect on the cultivation of Bradyrhizobium elsdenii, Flavobacterium, and Pseudomonas saccharophila, resulting in a decrease in the activity of Bradyrhizobium elsdenii, Flavobacterium, and Pseudomonas saccharophila. Ultimately, under the same soaking time, the biofilm formed in Comparative Examples 5-7 is not as complete as that in the examples, which in turn leads to a decrease in the effect of improving the survival rate of reeds.

[0086] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A wetland ecological restoration technology, characterized in that: The following steps are involved: Step 1), mixing Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophilus, nutrient substrate and water, and culturing for 7-9 days to obtain a culture solution; Step 2), cutting the reed rhizomes into segments and soaking them in a culture solution for 1-2 days to obtain pretreated rhizomes; Step 3), insert the pre-treated rhizomes into the soil covered with sewage for planting, and wait for the reeds to grow to purify the sewage.

2. A wetland ecological restoration technology according to claim 1, characterized in that: The mass ratio of the Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water is 0.01-0.02: 0.03-0.04: 0.02-0.03: 150-160: 1000.

3. A wetland ecological restoration technology according to claim 2, characterized in that: The nutrient substrate is a compound of glucose, peptone, sodium chloride, green plum juice and roxburghii juice.

4. A wetland ecological restoration technology according to claim 3, characterized in that: The mass ratio of the glucose, peptone, sodium chloride, green plum juice and rosa roxburghii juice is 40-42:35-37:15-17:33-35:27-29.

5. The wetland ecological restoration technology according to claim 1, characterized in that: In the step 1), Bradyrhizobium elsdenii, Flavobacterium, Pseudomonas saccharophila, nutrient substrate and water are mixed and cultured at a constant temperature of 36-38° C. for 7-9 days.

6. A wetland ecological restoration technology according to claim 1, characterized in that: In the step 2), the reed rhizomes are cut into segments and then immersed in a culture solution at a constant temperature of 36-38° C. for 1-2 days.

7. The wetland ecological restoration technology according to claim 1, characterized in that: In the step 2), the length of the reed rhizomes after cutting is 10-12 cm.

8. The wetland ecological restoration technology according to claim 1, characterized in that: In the step 3), the water depth of the soil covered with sewage for inserting the pre-treated rhizomes is 0.3-0.6 m.

9. The wetland ecological restoration technology according to claim 1, characterized in that: In step 3), the depth of the pretreated rhizomes inserted into the soil is 5-8 cm.

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