59 results about "Rhizobium sp." patented technology

The invention provides an artificial non-coding RNA (Ribonucleic Acid) molecule, a DNA (Deoxyribose Nucleic Acid) molecule, a biological material and application of the artificial non-coding RNA molecule, the DNA molecule and the biological material in improving the carbon and nitrogen metabolism capability of rhizobium, and belongs to the technical field of genetic engineering, the nucleotide sequence of the artificial non-coding RNA molecule is as shown in SEQ ID NO.1, and the nucleotide sequence of the DNA molecule transcribing the artificial non-coding RNA molecule is as shown in SEQ ID NO.2; the invention also provides a recombinant vector and recombinant rhizobium with efficient carbon and nitrogen metabolism capability. According to the artificial non-coding RNA molecule and the recombinant expression vector constructed by using the RNA molecule, the utilization capacity of malic acid of the rhizobium can be remarkably improved, then the symbiotic nitrogen fixation capacity of the rhizobium is enhanced, the rhizobium and leguminous crops are symbiotic, and the yield and quality of the leguminous crops can be remarkably improved.

The invention relates to a recombinant vector, genetic engineering rhizobium and application of the genetic engineering rhizobium to improvement of heat resistance of leguminous plants. The recombinant vector contains a thermal induction promoter PkatC and a cytokinin synthetic gene ipt, the nucleotide sequence of the thermal induction promoter PkatC is shown as SEQ ID NO.1, and the nucleotide sequence of the cytokinin synthetic gene ipt is shown as SEQ ID NO.2. The invention further discloses a preparation method of the recombinant vector. According to the method, a recombinant vector pSRK-PkatC-ipt containing a thermal induction promoter PkatC and a cytokinin synthetic gene ipt is transferred into sinorhizobium meliloti, genetic engineering rhizobium is constructed and obtained, and the high-temperature resistance of the sinorhizobium meliloti is enhanced by inoculating the genetic engineering rhizobium into a sinorhizobium meliloti plant. Compared with the prior art, the engineering bacterium constructed by the invention can effectively improve the survival and growth capability of alfalfa in a high-temperature environment, and the experimental result provides reliable technical support for breeding new stress-resistant alfalfa varieties and stable yield of ecological agriculture, and has important application value.

The present disclosure provides compositions for improved Rhizobium-mediated transformation in transformation of recalcitrant plants, and methods of use thereof. Also provided are compositions and methods for Rhizobium-dependent delivery of heterologous proteins directly to plant cells.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The present application relates to a kind of novel salt-tolerant rhizobium and its application, the rhizobium preservation number is GDMCC No:65918.The rhizobium of the application has host broad spectrum, can be symbiotic nodulation with leguminous plants including purple winged bean and peanut etc., improve the nitrogen fixation capacity of plant, and growth speed is faster.Especially, the rhizobium has high salt, acid, alkali tolerance, can grow in extreme salt, acid, alkali environment, improve soil, significantly promote the growth survival of plant in high salt, acid, alkali environment with symbiosis.The rhizobium of the application has very important application value to the development and utilization of leguminous plants, salt-alkali land (such as beach sand), ecological environment repair and improvement, and agricultural production.

The invention discloses a preparation method of a drought-resistant seedling-protecting rhizobium inoculant in the field of biological inoculant fertilizers. The method comprises the following steps: taking rhizobium, and performing fermentation culture to prepare a bacterial suspension; taking an organic carrier, doping the modified sea squirt cellulose nanocrystal dispersion liquid and a CaCl2 solution, and adsorbing the bacterial suspension on the organic carrier to obtain a bacterial material; the preparation method comprises the following steps: pretreating waste traditional Chinese medicine residues, performing oxygen-limited pyrolysis on part of the traditional Chinese medicine residues to obtain a carbon substance and pyrolysis condensate, mixing the carbon substance, the pyrolysis condensate and the remaining traditional Chinese medicine residues, and performing hydrothermal synthesis reaction to obtain a traditional Chinese medicine residue derivative carrier; adsorbing a liquid fertilizer on the traditional Chinese medicine residue derivative carrier to obtain a fertilizer; and uniformly mixing the fungus material and the fertilizer. Compared with a traditional liquid bacterial suspension, the rhizobium inoculant has the advantages that the problem that the rhizobium inoculant is permeated and lost after inoculation is avoided, the acting time of strains is prolonged, the contact symbiotic effect with root systems is improved, and the inoculation operation can be simplified; the liquid fertilizer can also assist in improving the drought resistance of the alfalfa seedlings and promoting the growth and development of the alfalfa seedlings.

The present application belongs to the field of microbial technology. More particularly, it relates to a salt-tolerant Bradyrhizobium sp. strain RY15 and its application. The Bradyrhizobium sp. strain RY15 provided by the present application has excellent salt tolerance and can significantly promote the growth and development and nodule fixation ability of Desmodium velutinum. The pot experiment results prove that the application of the RY15 strain can significantly increase the fresh weight of the aboveground part, the fresh weight of the underground part and the number of nodules of Desmodium velutinum; in the saline soil field, the yield of the pasture is increased by 68.89% after the inoculation of the RY15 inoculant. The RY15 strain of the present application can be made into a liquid or solid microbial inoculant, which is suitable for seed dressing, root soaking or soil application, and is simple to operate, low in cost and easy to popularize. The RY15 strain of the present application is a natural symbiotic bacterium, is safe and non-toxic, can improve the nitrogen cycle of soil, expand the planting of high-quality leguminous forage in saline-alkali soil, and has good application prospect and application value.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The invention discloses a method for efficiently screening rhizobium with functions of promoting growth and inhibiting aspergillus flavus and application of the method, the field ecological pre-enrichment effect of a microbial agent with an ARC function is introduced into a rhizobium screening process for the first time, and the abundance of a target flora is directionally increased from the source through the toxin control and bacterium enrichment effect of the rhizobium. The method comprises the following steps: synchronously collecting rhizosphere soil, root system and root nodule samples, and comprehensively separating rhizobium; a high-throughput primary screening system with parallel growth-promoting and antibacterial functions is constructed, and the nitrogen-fixing growth-promoting capability and the antibacterial effect of volatile substances of candidate strains are synchronously evaluated in an early stage through a pot experiment and a double-vessel buckling method. And further performing functional quantification, multi-gene phylogenetic identification and omics mechanism analysis on the positive strain. The method overcomes the defects that a traditional method is single in function and low in efficiency, and an effective way for rapidly, directionally and systematically screening multifunctional rhizobium resources from an optimized rhizosphere ecosystem is provided.

The application provides a preparation method and application of a complex microbial agent for promoting alfalfa growth, and relates to the technical field of agricultural microorganisms. The complex microbial agent is prepared by mixing a bacterial solution and a biological stimulant solution. The bacterial solution is a rhizobium solution, and the biological stimulant is a fucoidan solution. The volume ratio of the rhizobium solution to the fucoidan solution is 1:2-4. The complex microbial agent solves the bottleneck problem that a single rhizobium agent is unstable in actual application and is easily affected by the environment. The alfalfa treated by the complex microbial agent can improve the seedling emergence rate, plant height, biomass and stress resistance of the alfalfa.

The application provides an artificial non-coding RNA molecule, a DNA molecule and a biological material and application thereof in improving rhizobium carbon and nitrogen metabolic capacity, and belongs to the technical field of genetic engineering, wherein the nucleotide sequence of the artificial non-coding RNA molecule is shown as SEQ ID NO. 1, and the nucleotide sequence of the DNA molecule for transcribing the artificial non-coding RNA molecule is shown as SEQ ID NO. 2; the application also provides a recombinant vector and a recombinant rhizobium with high carbon and nitrogen metabolic capacity; the artificial non-coding RNA molecule and the recombinant expression vector constructed by using the RNA molecule provided by the application can significantly improve the utilization capacity of rhizobium malate, and further enhance the symbiotic nitrogen fixation capacity of rhizobium, and can significantly improve the yield and quality of legume crops in symbiosis with the legume crops.

The application discloses a kind of rhizobium preparation methods for drought resistance and seedling preservation in the field of biological bacterial agent fertilizer, and the method steps include: taking rhizobium fermentation culture to prepare bacterial suspension;Take organic carrier, incorporate modified sea moss cellulose nanocrystal dispersion and CaCl2 solution, then absorb bacterial suspension on organic carrier to obtain bacterial material;After pretreatment, part of the abandoned traditional Chinese medicine residue is oxygen-limited pyrolysis to obtain carbonaceous substance and pyrolysis condensate, then mix carbonaceous substance and pyrolysis condensate with the remaining traditional Chinese medicine residue, and carry out hydrothermal synthesis reaction to obtain traditional Chinese medicine residue derivative carrier;Take liquid fertilizer and adsorb it on traditional Chinese medicine residue derivative carrier to obtain fertilizer;Take bacterial material and fertilizer and mix evenly, and it is available.The rhizobium preparation compared with traditional liquid bacterial suspension can help to avoid the problem of penetration loss after inoculation, prolong the action time of bacterial strain, improve the symbiotic effect with root system, and also simplify the inoculation operation;Liquid fertilizer can also help to improve the drought resistance of alfalfa seedlings and promote their growth and development.

The invention relates to a soybean rhizobium sampling device, and belongs to the technical field of sampling devices.The soybean rhizobium sampling device comprises a sampling assembly and a cutting assembly, the sampling assembly comprises two sampling pieces, the adjacent sides of the opening ends of the two sampling pieces are hinged to each other, and first sliding grooves are formed in the top ends of the adjacent sides of the opening ends of the two sampling pieces; second sliding grooves are formed in the inner lower side walls of the adjacent sides of the opening end of the first sliding block. The cutting assembly comprises two sliding knives and two sliding rods. One ends of the two sliding knives slide in the two second sliding grooves correspondingly. The two sliding rods slide in the two first sliding grooves correspondingly and are fixedly connected with the corresponding sliding knives. The two sampling pieces hinged to each other are inserted into soil around the soybean root system, then the soil below the soybean root system is cut off through the sliding knife, the soil below the soybean root system is prevented from being adhered to the bottom end of the soybean root system, a traditional soybean root system pulling-out mode is replaced, the labor intensity of workers is reduced, and the soybean root system taking-out convenience is improved; and the soybean rhizobium can be conveniently picked by experimenters.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.

The application discloses a high-efficiency phosphorus-dissolving bio-organic fertilizer, which comprises phosphorus-dissolving bacteria and an organic matrix, and is characterized in that the mass ratio of Aspergillus niger and Rhizobium rhizagapanthus is 1:10 to 10:1; the Aspergillus niger is Aspergillus niger A44 with a preservation number of CGMCC No.11793; and the Rhizobium rhizagapanthus is Rhizobium rhizagapanthus 3T7 with a preservation number of CGMCC No.21507. The phosphorus-dissolving performance of the Aspergillus niger and the Rhizobium rhizagapanthus is synergistically improved, so that the availability of phosphorus in soil is improved, and the growth of plants is promoted.

The invention discloses a rhizobium mesonii strain APHNZY-24-4 which has the functions of promoting peanut growth and inhibiting aspergillus flavus, and the preservation number of the rhizobium mesonii strain APHNZY-24-4 is CCTCC (China Center for Type Culture Collection) M 2026228. According to the invention, integration of two functions of generating a specific bacteriostatic volatile matter, namely 3-methylbutyric acid, and efficient symbiotic nitrogen fixation on a single strain is realized in the rhizobium japonicum for the first time. 3-methylbutyric acid released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

A R gene is arranged for controlling soybean-rhizobia compatibility, wherein the GmNNL1 gene of the gene sequence in Hengfeng black bean of the soybean products is shown in SEQ ID NO. 2, and the encoded amino acid sequence is shown in SEQ ID NO. 3. The soybean R gene GmNNL1 is an effective gene that is able to regulate the nodule number of specific rhizobia on soybean, and to regulate the nodule number by directly recognizing the haplotype of the bradyrhizobia-specific effector protein NopP, which is able to limit the symbiotic nodulation of indigenous rhizobia, make soybean preferentially nodule with artificially applied high-efficiency rhizobia agents, and improve symbiotic nitrogen fixation capacity.

The invention provides rhizobium japonicum GY4 and application thereof, and relates to the technical field of microorganisms. Comprising soybean rhizobium GY4, the classification name of the soybean rhizobium GY4 is Rhizobium sp, the soybean rhizobium GY4 is preserved in Guangdong Microbial Culture Collection Center on July 15, 2025, the preservation number is GDMCC No. 66686, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The strain belongs to the rhizobium, can significantly improve the abundance of the rhizobium, promote the formation and development of root nodules, increase the number of the root nodules, improve the nitrogen fixation capability of the soybeans, and efficiently promote the growth of the soybeans under the strip relay intercropping condition of the soybeans and the corn, and provides a new strain resource for improving the nitrogen fixation efficiency in the soybean production.

The present invention relates to a composition comprising:an isolated endophyte, wherein the endophyte is a strain of Rhizobium metallidurans, andan adjuvant and wherein the Rhizobium metallidurans strain has Accession Number DSM34351.

The invention relates to the use of phlorotannins, in particular extracts from brown algae of the Fucaceae family, especially from the genera Fucus or Ascophyllum, to stimulate symbiosis between a plant and a mycorrhizal fungus or rhizobium. The invention also relates to a fertilizer product containing phlorotannins and its use in a plant treatment method.

The present disclosure relates to a modified plant SYMRK polypeptide that constitutively induces symbiotic organogenesis at a higher level than an unmodified plant SYMRK polypeptide under the same conditions, or induces symbiotic organogenesis in the absence of rhizobium and / or arbuscular mycorrhizal fungi recognized by a plant, and its use in a plant.

The invention discloses a protective agent of a microbial agent and application of the protective agent, and relates to the technical field of microbial preparations and agricultural application. The invention provides a protective agent of a microbial agent and application of the protective agent. And the protective agent is composed of polyvinylpyrrolidone and 1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid. The protective agent is applied to the rhizobium japonicum microbial inoculum. The protective agent provided by the invention has the best protective effect on microbial cells in the long-term storage process as long as 12 months at room temperature. The protective agent provided by the invention not only improves the survival rate of rhizobium under a storage condition, but also promotes the generation of extracellular polymeric substances, so that the viscosity of the preparation is increased, and the protective agent is more expected to be attached to the surface of a plant as a powerful adhesive and improve the colonization performance. In addition, the protective agent provided by the invention shows better nodulation ability when interacting with soybeans. The protective agent can significantly improve the long-term survival rate and storage stability of the rhizobium.

The application discloses a method for improving the yield of soybeans in saline-alkali soil by combined inoculation of rhizobium and arbuscular mycorrhizal fungi, which successfully constructs a "soybean-rhizobium-arbuscular mycorrhizal fungi" three-symbiotic system by combining Bradyrhizobium sp. with Endogone intraradices and Diversispora mosesii. The method can significantly promote the growth of soybeans, nodule fixation and nitrogen fixation, and mycorrhizal colonization. Under the condition that the application of nitrogen fertilizer is reduced by 26.00% and the application of phosphorus fertilizer is reduced by 23.08%, the yield is still increased by 29.96% and is 8.49% higher than that of the conventional fertilization group. Meanwhile, the method can enhance the oxidation resistance and nutrient absorption capacity of plants, improve the rhizosphere microenvironment, and effectively relieve the saline-alkali stress. The application provides a feasible technical path for the green and efficient production of soybeans in saline-alkali soil, helps to reduce the dependence on chemical fertilizers, and promotes the sustainable development of agriculture.

The application discloses a Neorhizobium rhizobium strain isolated and purified from soybean root nodules and application thereof. The strain is a new species of the Neorhizobium genus through physiological and biochemical determination and phylogenetic analysis, and is named as Neorhizobium glycine EC2-8. The strain re-inoculation experiment shows that the rhizobium strain can invade the soybean roots and form effective nodulation, and can promote the growth of soybeans.

The invention provides identification and application of symbiotic interaction receptor kinase of leguminous plants. The brand-new receptor-like kinase AMK2 (AM-induced kinases) which is formed by colonization of positive-tuned arbuscular mycorrhizal fungi and infection of negative-tuned rhizobium is disclosed for the first time, and the gene is induced by mycorrhiza and is necessary for symbiosis of mycorrhiza. And the AMK2 is inhibited by rhizobium, and the amk2 mutant has a phenotype with increased infection events. The invention discloses a new entry point for analyzing the specific regulation and control of the nodule symbiosis and mycorrhizal symbiosis signal transmission, and has important significance for deeply exploring the regulation and control mechanism of reciprocal symbiosis.

The invention provides a combined module for enhancing symbiotic nitrogen fixation capacity of rhizobium and application, and belongs to the technical field of synthetic biology. The combined module for enhancing the symbiotic nitrogen fixation capacity of the rhizobium comprises an inducible promoter element Pi and a transcriptional regulatory factor coding gene tetR, wherein the inducible promoter element Pi and the transcriptional regulatory factor coding gene tetR are sequentially connected; the inducible promoter element Pi specifically responds to a symbiotic nitrogen fixation signal; according to the invention, the combined module is inserted into an initial vector to construct a recombinant expression vector, and the recombinant expression vector is transferred into different soybean rhizobium chassis through a triparental conjugation method to obtain two nitrogen fixation recombinant engineering strains; the recombinant engineering strain can recover the influence of glyphosate stress on the symbiotic nitrogen fixation capability of the rhizobium.

The invention belongs to the technical field of microorganisms, and particularly relates to acid-resistant bradyrhizobium capable of degrading nitrous oxide and application of the acid-resistant bradyrhizobium. The invention relates to acid-resistant bradyrhizobium beet, which is bradyrhizobium beet purchased in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the acid-resistant bradyrhizobium beet is CGMCC 1.15594. The acid-resistant bradyrhizobium can still keep a good growth state and physiological activity under the conditions of low pH value and no assistance of an external carbon source. After the fertilizer is applied to farmland, stable colonization can be realized, the pH value of soil can be effectively buffered and increased through the metabolic activity of the fertilizer, and the rhizosphere microenvironment of crops is improved; meanwhile, the biological immobilization capacity of soil microbial communities on nitrogen is enhanced, inorganic nitrogen can be intercepted, and a biological basis is provided for agricultural non-point source pollution prevention and control, nitrogen fertilizer loss reduction and chemical fertilizer application reduction and synergism.

The invention provides application of cellooligosaccharide in inducing plant PTI to promote rhizobium recruitment and symbiotic nodulation, and belongs to the technical field of plant-microorganism interaction. The invention provides an application of cellooligosaccharide in inducing plant innate immunity, cellooligosaccharide with structure specificity is used for inducing PTI signals, and accurate targeting recruitment of rhizobium is carried out to improve the nodulation nitrogen fixation and growth performance of plants. The invention further provides a method for promoting plant growth, the compatibility state of plant growth and immunity is maintained under PTI activation only through PTI stimulation of the cello-oligosaccharide, and the nodulation efficiency and yield of crops are effectively improved by establishing a positive feedback model of PTI immune activation-microbial interaction-nutrition enhancement-growth promotion. According to the method, the in-situ symbiotic rhizobium can be accurately enriched without supplementing an exogenous microbial agent, and the method is an in-situ microorganism regulation and control method which is high in ecological adaptability and high in field generalizability.

A bacterial culture is disclosed which comprises Rhizobium bacteria having a nucleic acid sequence at least 99% identical to the nucleic acid sequence as set forth in SEQ ID NO: 1. Uses thereof are also disclosed.