147 results about "Rhizobium sp." patented technology

The invention discloses a method for accelerating aerobic sludge granulation by the aid of charcoal. The method includes that pyridine degradation bacteria Rhizobium sp. NJUST18 and ordinary activated sludge are used as compound inocula for the aerobic granular sludge, the charcoal is used as a crystal nucleus for the aerobic granular sludge in a sequencing batch reactor form, and quick forming of the pyridine degradation aerobic granular sludge can be promoted. The method has the advantages that the aerobic granular sludge with a pyridine degradation function can grow by the aid of pyridine which is a unique carbon source and a unique nitrogen source, granules have regular shapes and are good in sedimentation after the cultivated granular sludge is mature, reaction systems are high in sludge concentration, the high-concentration pyridine can be degraded and is subjected to synchronous nitrosation, and the cultivation time of the aerobic granular sludge for treating wastewater difficult to biodegrade can be effectively shortened.

The invention discloses a composite microbial inoculum, and an immobilization method and application thereof. The composite microbial inoculum is prepared from an Ochrobactrum sp.B3 mycetocyte microbial suspension and a Rhizobium sp.T3 mycetocyte microbial suspension, wherein the concentration of the Ochrobactrum sp.B3 mycetocyte microbial suspension is 0.15-0.2g/L, and the concentration of the Rhizobium sp.T3 is 0.15-0.2g/L. The immobilization method of the composite microbial inoculum has the advantages of simple technique, and cheap and accessible raw materials, and is beneficial to large-scale production. The composite microbial inoculum immobilized cell granules have the advantages of high mechanical strength, high elasticity and stable enzymic activity, have certain toxin immunity in a reaction system, have strong tolerance to temperature and pH value, and can still efficiently degrade H2S waste gas after being used repeatedly.

The invention discloses a bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and an application of the bacterium. The bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim is rhizobium sp. LD1616 and the preservation number of the bacterium is CGMCC (China General Microbiological Culture Collection Center) No.7775. The rhizobium sp. LD1616 with the preservation of CGMCC NO.7775, disclosed by the invention, has the advantages that the degradation rate of the bacterium to chlorimuron-ethyl (45mg/L) is up to 71.56% in 7 days in an inorganic salt culture medium and the degradation rate of the bacterium to carbendazim (100mg/L) is up to 24.92% in 5 days, which indicates that the bacterium is capable of efficiently degrading the chlorimuron-ethyl and the carbendazim and has a broad application prospect in the remediation of soil polluted by residual pesticides chlorimuron-ethyl and carbendazim.

The invention relates to rhizobium astragula capable of effectively fixing nitrogen and a culture method and application thereof. The rhizobium astragula CGMCC No.4347 is cultured by the steps of strain activation, seed culture, fermentation culture and the like. The invention has the following beneficial effects: when the fermentation culture medium improved by the invention is adopted for culturing, the viable count of the fermentation liquor is far higher than that in the prior art; compared with the bacteria which are not inoculated on astragalus sinicus, the rhizobium astragula agent can ensure the fresh weight of the astragalus sinicus plants to be increased by more than 241%, the nitrogen content of the plants to be increased by more than 86% and the yield to be increased by more than 47% after being inoculated on the astragalus sinicus; compared with the existing produced strains, the rhizobium astragula agent ensures the fresh weight of the astragalus sinicus plants to be increased by more than 58%, the nitrogen content of the plants to be increased by more than 18% and the yield to be increased by more than 12%, thus being an excellent strain for production of the rhizobium astragula and inoculation of the astragalus sinicus; and the culture method is simple and practical, and the raw materials have wide sources and low price and are suitable for large-scale industrialized production.

The invention relates to a rhizobium strain, namely Rhizobium sp. Ery-6). The Rhizobium sp. Ery-6 is preserved at China Center for Type Culture Collection (CCTCC) under the preservation number of CCTCC M 2019729. The invention further provides a microbial agent containing the Rhizobium sp. Ery-6 and a preparation method of the microbial agent; and moreover, the invention still further provides application of the microbial agent in erythromycin degradation. The Rhizobium sp. Ery-6 provided by the invention has very strong degradation ability on erythromycin, so that the Rhizobium sp. Ery-6 cangrow by taking the erythromycin as the sole carbon source. In 48 hours, the Rhizobium sp. Ery-6 is capable of reaching an erythromycin degradation rate up to 79.78% on erythromycin of which the concentration is 100 mg/L in an inorganic salt medium as well as an erythromycin degradation rate up to 37.3% on erythromycin of which the concentration is 1000 mg/L in an organic salt medium; and thus, theRhizobium sp. Ery-6 has a good development and application prospects.

The invention discloses a rhizobium strain and its application. The rhizobium strain is named as Rhizobium sp., the strain number is zb612, and preservation number is CGMCC No. 10721. The rhizobium strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) at the No.3, 1 West Beichen Road, Chaoyang District of Beijing on April 15th, 2015. The strain provided by the invention can be used in onsite real nitrogen removal from sewage so as to raise the effect of removing ammonia nitrogen from oil sludge. The rhizobium provided by the invention has the characteristic of removing ammonia nitrogen. According to the rhizobium strain, organic carbon can be used as the sole carbon source and ammonia nitrogen is used as the sole nitrogen source so as to carry out metabolism, and ammonia nitrogen is directly converted to N2 through heterotrophic nitrification-aerobic denitrification so as to finish nitrogen removal. Thus, problems of aerobiotic nitrification and anoxic denitrification sectional treatment in traditional biological removal of nitrogen are solved.

The invention relates to a nutrient film protecting agent of rhizobium japonicum and a production method thereof. The nutrient film protecting agent of rhizobium japonicum is prepared by selecting the raw materials of PVP (Polyvinylpyrrolidone) K30, PVP S630, sodium carboxymethyl cellulose, peptone, bovine serum albumin and sodium molybdate and reacting under a certain reaction condition; the nutrient film protecting agent of rhizobium japonicum, a liquid rhizobium japonicum agent and soybean seeds are mixed and stirred according to a certain proportion; and after the soybean seeds are coated and stored for two days, each soybean seed can ensure 100,000 live rhizobium japonicums. The invention has wide needed raw material sources, simple production process and low cost, can play roles of nutrition, film formation and protection in the rhizobium japonicum, and can ensure that the liquid rhizobium japonicum agent is preserved for one year at normal temperature; the live bacterium quantity is not smaller than 2,000,000,000/ml, and the nitrogen fixing activity is not reduced.

The invention discloses a rhizobium strain and an application thereof. The rhizobium strain is rhizobium indigoferae NM60 of which the CGMCC ((China General Microbiological Culture Collection Center) No is 4647. Rhizobium indigoferae NM60 can promote the growth of different kinds of hairy vetches, the strain has better symbiotic nodulation capability with each kind of hairy vetch, the strain is determined as the broad-spectrum nodulation strain of vetch and can be used to prepare green manure; and the strain has higher practicability and popularization and has a wide application prospect in the planting of vetch.

The invention relates to the field of agricultural microorganisms, and in particular relates to a rhizobium pusense capable of degrading apple autotoxin phlorizin and application thereof. A rhizobiumpusense YIC4105 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 2rd, 2017 with the preservation number of CGMCC No.14852. The bacterial strain is separatedfrom sesbania rhizosphere soil naturally distributed at the Yellow River delta and is obtained through artificial separation and purification; the bacterial strain has relatively strong capability ofdegrading phlorizin and can be stabilized and colonized at rhizosphere of apples; and successive cropping obstacle of an apple seedling caused by accumulation of phlorizin can be effectively preventedby utilizing the bacterial strain, so that the rhizobium provided by the invention has good prospect of being developed into a microbiological inoculants or bacterial fertilizer capable of promotingcrop growth.

The invention discloses rhizobium sp. T3 which is preserved in China typical culture collection center, the preservation date is April, 2nd 2011, the preservation number is CCTCC No.M2011105, and the preservation address is Wuhan University in Wuhan, China. The application comprises the following step: by taking hydrogen sulfide as the only sulfur source and energy source, culturing the bacterial liquid obtained from the amplification culture of rhizobium sp.T3 selectively for 2-3 days at the temperature of 25-40 DEG C under an enclosed condition with the pH of 4.0-9.0, thus hydrogen sulfide is resolved, wherein the concentration of bacterial liquid cells is 200-300mg/L, and the initial concentration of hydrogen sulfide is 150-600ppm. The bacterial strain provided by the invention has efficient and high-tolerance hydrogen sulfide degradation capability, also can adapt to complex actual conditions, and has wide application prospects.

The invention discloses a special bacterial fertilizer for sainfoin, and belongs to the technical field of microorganisms. The special bacterial fertilizer for sainfoin comprises a strain of Rhizobiumyanglingense GAU-00008, a strain of Bacillus sp. CHO87, a stain of Bacillus subtilis LSH11 and a vector. According to the invention, through a synergistic effect of the three strains, sufficient effective root nodules can be formed at root parts of sainfoin; and meanwhile, soil is activated, so that absorption of crops to other nutrient elements is promoted, a nutrition structure of plants is improved, growth of the plants is regulated and promoted, and disease resistance of the plants is enhanced.

The invention discloses a rhizobium sp. WP 1-3, and the preservation number is CGMCC No.14472. In addition, the invention further discloses the application of the rhizobium to decomposition of potassium in soil and decomposition of insoluble phosphorus in the soil. The rhizobium is a dominant strain screened by taking potash feldsparf as a potassium source, the strain is capable of transforming the insoluble potassium in the aluminosilicate in the soil into a soluble component in the processes of growth and propagation, and promoting the decomposition of the insoluble potassium to increase thesource of the phosphorus in the soil. In addition, the strain further has an effect of fixing nitrogen, and provides the necessary nitrogen for the growth and metabolism of a plant.

The invention discloses preparation and application of a novel alfalfa rhizobia immobilized embedded granule formation, and belongs to the field of agricultural microbe application. An immobilized cell technology is a method for immobilizing microbial cells in some special materials, preparing granules to form a limited space, enabling the thallus to keep biological activity in a certain time and realizing proliferation. At present, the alfalfa rhizobia is widely applied to the fields such as medicines, foods, fermentation industry, wastewater treatment and the like, preliminary study in a rhizobium japonicum embedding aspect is realized, while the preparation and application of the alfalfa rhizobia embedded immobilized granules is still a blank at present. According to the novel alfalfa rhizobia immobilized embedded granules developed in the invention, the alfalfa rhizobia is conveniently added by alfalfa cultivation enterprises, and the activity of fungicides is not lost in the compounding, processing, granulating and transportation and storage processes. Therefore, alfalfa rhizobia bacterial manure can be easily used by farmer households, and the main technical bottleneck difficulty of a high-tolerance high-activity alfalfa rhizobia seed embedding technique is broken.

The present invention belongs to the fields of agriculture and agricultural technology and Rhizobium applications, and discloses new applications of a Rhizobium sp. W33 strain. According to the present invention, the Rhizobium sp. W33 is adopted as a raw material, and bacterial activation is performed to prepare a bacterial suspension, wherein the bacterial suspension is inoculated in a potassium-containing liquid culture medium so as to absorb the potassium in the solution, and the bacterial suspension is inoculated in soil so as to reduce potassium fertilizer leaching loss, significantly promote plant growth and absorption on potassium and phosphorus, and improve potassium fertilizer absorption and utilization rate.

The invention provides a selenized microbial exopolysaccharide and a preparation method thereof. The preparation method comprises the steps of: adding micromolecular Rhizobium sp. exopolysaccharide to nitric acid solution, stirring, adding selenous acid and barium chloride, adding sulfuric acid dropwise, removing precipitates by centrifugation, regulating the pH of supernatant to 7 by sodium hydroxide, putting the supernatant in a dialysis bag for dialysis, concentrating the dialysate under reduced pressure and then adding edible alcohol, separating out selenized microbial exopolysaccharide, and collecting and freeze-drying the selenized microbial exopolysaccharide to obtain selenized micromolecular microbial exopolysaccharide. The content of selenium in the exopolysaccharide can reach 586 to 790 mu g/g according to measurement; mice tests show that the exopolysaccharide has excellent antitumor activity; and the exopolysaccharide can be used in preparing antitumor medicines and healthcare products.

The invention discloses a rhizobium and application thereof. The rhizobium provided by the invention is northern Mesorhizobium septentrionale CCBAU 03524, of which the preservation number in the China General Microbiological Culture Collection Center is CGMCC No.11015. Experiments show that the northern Mesorhizobium septentrionale CCBAU 03524 CGMCC No.11015 has important application value in promoting growth of astragalus plants and/or improving quality of astragalus and/or raising seedlings of astragalus plants; the promotion of the growth of astragalus plants is reflected as increase of the number of nodules and/or increase of dry weight of underground parts; the improvement of the quality of astragalus is reflected as increase of percentage of astragalus polysaccharide in astragalus root.

A method of purifying waste water biologically by using three particularly suitable bacteria: Bacillus sp. DT-1, Pseudomonas azelaica, DT-2, and/or Rhizobus sp. DT-5, or mixed populations thereof. The invention further relates to the bacteria and the mixed populations and use thereof in purifying waste water. The invention further relates to a bioreactor including the bacteria.

The invention belongs to microbial fermentation, and particularly relates to rhizobium pumilum and an application of the rhizobium pumilum in preparation of water-soluble beta-1,3 glucan. A method comprises the following process steps: inoculating rhizobium pumilum with the preservation number of CGMCC No.19764 into a sterilized culture solution containing a carbon source, a nitrogen source and other necessary nutrient substances, ventilating, stirring and fermenting at the temperature of 28-32 DEG C, and finishing the fermentation when the fermentation period does not exceed 70 hours to prepare fermentation liquor containing beta-1, 3 glucan. The method solves the problems of tedious process, low product yield and the like in the prior art, and the product has the advantages of high yield, high product viscosity, good water solubility, low fermentation cost and the like.

The invention discloses rhizobium named as Rhizobium sp. NPLJ-2. The Rhizobium sp. NPLJ-2 is preserved in China Center for Type Culture Collection on October 19th, 2020 with the preservation number ofCCTCC M 2020603. The invention also discloses application of the rhizobium in degrading nonyl phenol in a water environment. The rhizobium disclosed by the invention has the advantages that the biodegradation rate of the nonyl phenol with the initial concentration of 100 mg/L in a water body under an aerobic condition can reach 80% or above, the biodegradation time is effectively shortened, and meanwhile, COD contributed by the nonyl phenol in sewage is also effectively reduced; and the nonyl phenol in the sewage is quickly and efficiently degraded by utilizing a biological method, and the rhizobium has important significance for treatment of high-concentration environmental hormone-nonyl phenol in the sewage.

The invention discloses a method for improving conjugational transfer efficiency of fluorescent plasmid transfer of fluorescence labeling strains of rhizobia by taking sterilizing alfalfa leaves as triparental mating carriers instead of sterile filter membranes. The method can be used for efficiently constructing CFP (cyan fluorescent protein) gene labeled strains of rhizobia. The method specifically comprises the following steps of taking small and tender leaves of alfalfa, uniformly putting the leaves in a single layer in a culture dish after washing the leaves with distilled water, and sterilizing the leaves; taking out the leaves with a pair of sterile tweezers, sticking the leaves to the surface of TY culture media, and covering the culture dish; and using the leaves to replace microporous filter membranes in the triparental mating process to carry out mixed growing culture on donors, assistant bacteria and recipient bacteria. Compared with the microporous filter membranes as the mating carriers, the alfalfa leaves, as the mating carriers, can improve the conjugational transfer efficiency of the fluorescent plasmids by 8.4-11 times, and the difference is obvious. As the used raw materials are alfalfa plant leaves which are renewable and accessible, the method has the advantage that the raw materials are cheap and accessible, is simple and convenient to operate and is suitable for constructing the fluorescence labeling strains of rhizobia.

The invention provides a nitrogen fixing bacterium in mangrove rhizosphere (DZY-N33) and an application thereof. The settlings and rhizosphere soil of the marine mangrove ecological system are bred selectively and in enrichment; a new nitrogen fixing bacterium is obtained by adopting the conventional microorganism separating and purifying methods, that is rhizobium sp. DZY-N33 strain which is stored in CCTCC (China Center for Type Culture Collection) on April 13th, 2007; the CCTCC is located in Wuhan University, Wuhan, China; the collection number is M207045. The strain has high biological nitrogen fixing activity; the microbial inoculum with the bacterium is used to breed mangrove plants such as rhizophora stylosa and bruguiera, which can evidently promote the growth of plant, thereby being capable of preparing the biological nitrogen fixing bacterium fertilizer promoting the growth of mangrove plants.

The invention provides rhizobium tianshanense transcriptional regulation protein MsiR mutant protein and application thereof in a canavanine biosensor, the mutant protein is non-natural protein, and the mutant protein has the capacity of specifically responding to canavanine. The mutant protein is mutated in one core amino acid related to canavanine binding of wild type MsiR: the 133th site is mutated into alanine (A) from aspartic acid (D), the protein sequence of the mutant protein is shown in SEQ ID NO.1, and the nucleotide sequence of the mutant protein is shown in SEQ ID NO.2. Compared with the wild type, the fluorescence value of the mutant protein in response to canavanine is improved by two times, the affinity of the binding region of the C-terminal effector of the MsiR mutant protein and canavanine is improved by 1.5 times; and meanwhile, the regulation and control activity of the MsiR transcription regulation and control protein is improved by screening the mutant protein of the protein.

The invention discloses a Rhizobium sp. WYJ-E13 and its application in the preparation of a Curcuma wenyujin growth promoter. Curcuma wenyujin tissue culture seedlings grow fast and have many branchesand roots on a medium inoculated with a WYJ-13 bacterial solution, so the WYJ-13 has a Curcuma wenyujin tissue culture seedling growth promotion effect.