136 results about "Rhizobium sp." patented technology

The invention provides a microbial agent for promoting growth of medicago sativa and application of the microbial agent, and relates to the technical field of agriculture, the microbial agent is prepared from sinorhizobium meliloti MCSWL1, sinorhizobium meliloti MCSD1 and nitrogen-fixing rhizobium sp. MCDC3 according to the mass ratio of 1: 1: 2, and the mass ratio of the sinorhizobium meliloti MCSWL1, the sinorhizobium meliloti MCSD1 to the nitrogen-fixing rhizobium sp. MCDC3 to the nitrogen-fixing rhizobium sp. MCDC3 to the nitrogen-fixing rhizobium sp. MCDC3 is 1: 1: 2. The microbial agent for promoting the growth of the medicago sativa, provided by the invention, can form a close symbiotic relationship with the medicago sativa (particularly grassland No.3), and provides sufficient nitrogen nutrition for the growth of the medicago sativa, so that the soil fertility is effectively improved, and the healthy growth of plants is promoted.

The invention provides hyaluronic acid and a preparation method thereof, and belongs to the technical field of microorganisms. According to the invention, hyaluronic acid is generated by rhizobium pustatae QY124aN, so that the problems of high strain culture cost in the prior art and no technical method for producing medium-molecular-weight hyaluronic acid from rhizobium pustatae are solved. The content of hyaluronic acid in the hyaluronic acid dry powder prepared by the method is 57.68%, and the hyaluronic acid dry powder is safer than hyaluronic acid prepared by streptococcus zooepidemicus in the prior art.

The invention belongs to the technical field of microorganisms, and particularly relates to rhizobium jangularis LN-ZDJ-1, a complex microbial inoculant containing the rhizobium jangularis LN-ZDJ-2 and application of the rhizobium jangularis LN-ZDJ-1 and the complex microbial inoculant. The rhizobium jangularis LN-ZDJ-1 is preserved in the China Center for Type Culture Collection on July 22, 2024, and the preservation number of the rhizobium jangularis LN-ZDJ-1 is CCTCC (China Center for Type Culture Collection) NO: M20241652; the bacillus amyloliquefaciens LN-ZDJ-2 is preserved in the China Center for Type Culture Collection on July 22, 2024, and the preservation number of the bacillus amyloliquefaciens LN-ZDJ-2 is CCTCC (China Center for Type Culture Collection): M 20241651; the compound microbial agent provided by the invention not only can remarkably improve the growth and yield of the Achyranthes bidentata, but also can improve the nutrient absorption capacity of plants, further promotes the healthy growth and resistance of the plants by secreting active substances such as auxin and siderophores, and is beneficial to improving the quality of pasture.

The invention discloses a rhizobium bacterium and application of the rhizobium bacterium in degradation of 1, 4-dioxane, and belongs to the technical field of environmental microorganisms. The bacterial classification of the rhizobium is named as rhizobium sp., and the rhizobium is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.25779. The invention further discloses a preparation method of the rhizobium. The strain can rapidly degrade 1, 4-dioxane, and has the advantages of strong tolerance, wide applicable pH range, wide applicable concentration range for degradation, wide applicable environmental condition range and strong pollutant tolerance. And by using the strain as a strain resource, economic, green and efficient microbial remediation of 1, 4-dioxane pollution can be realized.

The invention relates to the technical field of ecological restoration, and particularly discloses a wetland ecological restoration technology. The wetland ecological restoration technology comprises the following steps: step 1) mixing bradyrhizobium angularis, flavobacterium, pseudomonas saccharophila, a nutrient substrate and water, and culturing for 7-9 days to obtain a culture solution; the method comprises the following steps of 1, preparing a culture solution, 2, cutting reed rhizomes into segments, and soaking the segments in the culture solution for 1-2 days to obtain pretreated rhizomes, and 3, inserting the pretreated rhizomes into sewage-covered soil for planting until the reeds grow to purify the sewage. The method has the advantages that the survival rate of the reeds in the sewage is increased, and wetland ecological restoration is accelerated.

The invention relates to the technical field of microorganisms, and provides a soybean rhizobium and application of the soybean rhizobium, the soybean rhizobium is Chinese rhizobium freudeneri HY47, and the preservation number of the soybean rhizobium is CCTCC (China Center for Type Culture Collection) NO: M 20242695. The Sinorhizobium fredii HY47 has a stable symbiotic nitrogen fixation effect and a high IAA secretion capability, can maintain the growth activity in a soil pH value range of 5-9, can stably grow at 42 DEG C, and is suitable for being used as an excellent strain for increasing the yield of soybeans in Jianghan plain areas.

The invention belongs to the technical field of agriculture and relates to microorganisms, in particular to a compound microbial agent and application thereof. The invention relates to a compound microbial agent, which is prepared from slow-growing rhizobium HS3, pseudomonas Y43, burkholderia Y30 and agrobacterium Y27. The compound microbial agent NBI prepared from efficient rhizobium and beneficial microbial strains adapting to local soil is preferably selected by combining soil types and microbial adaptability of different regions in Hainan province, and can be used for improving nodulation and nitrogen fixation capacity of fresh soybeans, promoting the yield of the soybeans, reducing application of chemical fertilizers and improving the per unit yield of the soybeans.

The invention relates to the technical field of agricultural microorganisms, in particular to hopane synthetic gene combination and application thereof in enhancing environmental stress resistance of broad-host rhizobium, and particularly relates to 1) regulation and control of sucrose osmotic pressure stress tolerance of rhizobium; (2) regulating and controlling the salt stress tolerance of the rhizobium; 3) regulating and controlling the adaptive capacity of the rhizobium to high temperature; and (4) regulating and controlling the alkaline environment adaptability of the rhizobium.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses bradyrhizobium japonicum and application thereof. The bradyrhizobium japonicum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 15, 2023, and the preservation number is CGMCC No.29321. The bradyrhizobium japonicum is named as Bradyrhizobium japonicum. The invention also provides a culture, a fermentation broth, a bacterial suspension, a bacterial agent and bacterial powder of the strain. The invention further discloses application of the bacterial strain and the culture, the fermentation liquor, the bacterial suspension, the bacterial agent and the bacterial powder of the bacterial strain in increasing the number of soybean nodules and promoting soybean plant growth. The bradyrhizobium japonicum provided by the invention has a relatively good nodulation effect and can effectively promote plant growth.

The invention discloses a microbial complex microbial inoculant, a preparation method thereof and application of the microbial complex microbial inoculant in red mud treatment, and relates to the technical field of mine ecological restoration. The invention provides a composite microbial inoculant which comprises a microbial inoculant microorganism A, a microbial inoculant microorganism B and a microbial inoculant microorganism C in a weight ratio of (1-3): (1-4): 1, the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial agent microorganism B is at least one of desulfurization vibrio, streptococcus, acetobacter and acidophilic thiobacillus; the microbial agent microorganism C is at least one of agrobacterium, enterobacter, streptomyces and klebsiella. The microbial agent disclosed by the invention can effectively solve the problems of high salt and alkali content of the red mud and heavy metal toxicity, and also can create conditions suitable for plant growth on the red mud, so that the ecological restoration effect is improved.

The invention belongs to microbial fermentation, and particularly relates to rhizobium massa and application of rhizobium massa in preparation of selenium-rich beta-1, 3 / alpha-1, 3-glucan fermentation liquor. The preparation method comprises the following steps: inoculating rhizobium pustatae of which the Latin name is Rhizobium push and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34553 into a sterile fermentation culture medium containing inorganic selenium, and carrying out aerated fermentation, thereby obtaining the selenium-enriched rhizobium pustatae. The method solves the problems that in the prior art, a fermentation strain is low in tolerance to selenium element, the capacity of converting inorganic selenium into organic selenium is weak, and the like, and has the advantages that the product yield and the fermentation liquor viscosity are high, and the selenium content in the selenium-rich beta-1, 3 / alpha-1, 3-glucan product is high.

The invention provides an artificial non-coding RNA (Ribonucleic Acid) molecule, a DNA (Deoxyribose Nucleic Acid) molecule, a biological material and application of the artificial non-coding RNA molecule, the DNA molecule and the biological material in improving the carbon and nitrogen metabolism capability of rhizobium, and belongs to the technical field of genetic engineering, the nucleotide sequence of the artificial non-coding RNA molecule is as shown in SEQ ID NO.1, and the nucleotide sequence of the DNA molecule transcribing the artificial non-coding RNA molecule is as shown in SEQ ID NO.2; the invention also provides a recombinant vector and recombinant rhizobium with efficient carbon and nitrogen metabolism capability. According to the artificial non-coding RNA molecule and the recombinant expression vector constructed by using the RNA molecule, the utilization capacity of malic acid of the rhizobium can be remarkably improved, then the symbiotic nitrogen fixation capacity of the rhizobium is enhanced, the rhizobium and leguminous crops are symbiotic, and the yield and quality of the leguminous crops can be remarkably improved.

The invention relates to a recombinant vector, genetic engineering rhizobium and application of the genetic engineering rhizobium to improvement of heat resistance of leguminous plants. The recombinant vector contains a thermal induction promoter PkatC and a cytokinin synthetic gene ipt, the nucleotide sequence of the thermal induction promoter PkatC is shown as SEQ ID NO.1, and the nucleotide sequence of the cytokinin synthetic gene ipt is shown as SEQ ID NO.2. The invention further discloses a preparation method of the recombinant vector. According to the method, a recombinant vector pSRK-PkatC-ipt containing a thermal induction promoter PkatC and a cytokinin synthetic gene ipt is transferred into sinorhizobium meliloti, genetic engineering rhizobium is constructed and obtained, and the high-temperature resistance of the sinorhizobium meliloti is enhanced by inoculating the genetic engineering rhizobium into a sinorhizobium meliloti plant. Compared with the prior art, the engineering bacterium constructed by the invention can effectively improve the survival and growth capability of alfalfa in a high-temperature environment, and the experimental result provides reliable technical support for breeding new stress-resistant alfalfa varieties and stable yield of ecological agriculture, and has important application value.

The present disclosure provides compositions for improved Rhizobium-mediated transformation in transformation of recalcitrant plants, and methods of use thereof. Also provided are compositions and methods for Rhizobium-dependent delivery of heterologous proteins directly to plant cells.

This invention belongs to the field of agricultural technology and relates to microorganisms, particularly Sinorhizobium rhizobium Y18, a bacterial agent, a coating agent, and their applications. It was deposited with the Guangdong Provincial Microbial Culture Collection on April 7, 2025, under the accession number GDMCC No. 66071. The address of the deposit is the Institute of Microbiology, Guangdong Academy of Sciences, Building 59, 100 Xianlie Middle Road, Guangzhou. This strain exhibits broad-spectrum nodulation properties and effectively promotes soybean nodulation and growth, thus possessing excellent practical application value.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The invention provides a strain of rhizobium pustatae QY124aN and a microbial inoculum, and belongs to the technical field of microorganisms. By providing the rhizobium pustatae QY124aN and the fungicide, the problems that a culture medium required by existing rhizobium pustatae is complex in nutrient substance, and the stability of the rhizobium pustatae fungicide is poor are solved. The rhizobium massa QY124aN provided by the invention is separated from an organic fertilizer, has a self-living nitrogen fixation capability, needs simple nutrient substances, can degrade corn straw lignin, increase the water solubility of the corn straw lignin, enhance the antioxidant activity and uvioresistant activity of metabolites, has a protection effect on plant roots, promotes plant growth, and has a good application prospect. The stress resistance of plants in agricultural production is improved. And hyaluronic acid can be produced, the viscosity of fermentation liquor can be increased, and the stability of the rhizobium pustatae QY124aN microbial inoculum is facilitated.

This invention discloses a genetically engineered rhizobium strain overexpressing purine nucleoside phosphorylase, its construction method, and its applications, belonging to the fields of agricultural biotechnology and genetic engineering. This invention uses *Rhizobium fischeri* (Fragariae spp.) as the basis for its development. Sinorhizobium fredii CCBAU 45436 was used as the starting strain. The strain was introduced by introducing a sample containing the strain itself. rpoD Strong promoter sequences of genes, encoding purine nucleoside phosphorylases deoD The recombinant strain CCBAU 45436 deoD OE was obtained by using a recombinant plasmid composed of the gene sequence and the gene encoding a 3×Flag tag sequence. This recombinant strain has the accession number CGMCC No. 38578 at the China General Microbiological Culture Collection Center (CGMCC). This invention experimentally demonstrates that inoculation... deoD deoD deoD deoD deoD deoD deoD deoD Soybeans overexpressing the recombinant strain (CGMCC No. 38578) showed significantly better plant height and root nodule fresh weight than soybeans inoculated with unmodified wild-type rhizobium strains.

The invention discloses a method for promoting non-leguminous plants infected by rhizobium through soybean RALF protein and application of the soybean RALF protein. The amino acid of the RALF protein is shown as SEQ ID No: 1, and the related GmRALF9 small peptide sequence of the RALF protein is shown as SEQ ID No: 9; the GmRALF9 small peptide treatment changes the root hair forms of arabidopsis thaliana and rice, specifically, the top end is deformed and expanded; the GmRALF9 small peptide is used for treatment, infection and colonization of arabidopsis thaliana root hair cells by the rhizobium are finally achieved, the method is the first step of symbiotic nodulation, and a new thought and method are provided for research on symbiotic nitrogen fixation of non-leguminous plants.

The present invention relates to the field of agricultural microbial technology, and specifically discloses a method for recruiting root endophytic fungi by a rhizobium GZHC2-2 strain and its application in alleviating sensitive soybean waterlogging stress. The rhizobium (Rhizobium multihospitium) GZHC2-2 strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on January 4, 2023, with a deposit number of GDMCC No.63032. This strain has the ability to help soybeans resist waterlogging. Inoculation of the rhizobium under waterlogging stress can increase the aboveground and underground biomass, the number of nodules and the fresh weight of nodules of soybeans. At the same time, the ability of waterlogging-sensitive soybean varieties to enrich beneficial root endophytic fungi (such as Sordariomyceta, Moesziomyces, Rhynchogastremataceae, Eurotiomycetes, Ustilaginaceae) is induced. After purifying and isolating the root endophytic fungi, the enriched beneficial fungi can also significantly increase the aboveground and underground biomass, the number of nodules and the fresh weight of nodules of soybeans under waterlogging stress. The invention can be used to improve the growth and increase the yield of soybeans under waterlogging stress, and has broad application prospects.

The utility model relates to the field of rhizobium culture, in particular to an efficient culture device for soybean rhizobium. According to the efficient culture device for the rhizobium japonicum, materials can be sprayed to soybean plants, nutrient solutions can be conveniently absorbed by the rhizobium japonicum, and the uniformity and the convenience of spraying the materials to the soybean plants are improved. A soybean rhizobium efficient culture device comprises a containing box, a connecting plate, a water outlet plate and the like, the connecting plate is connected into the containing box, and the water outlet plate is connected to the right side of the middle of the containing box. According to the soybean plant watering device, through rotation of the lead screw, the supporting block moves front and back under the action of threads, then the watering frame is driven to move front and back, a nutrient solution makes contact with soybean plants, the soybean plants can be watered, and rhizobium can absorb the nutrient solution conveniently; and the effects of uniformity and convenience of soybean plant sprinkling are improved.

The invention relates to the technical field of microorganisms, and provides a rhizobium mutant and application thereof, the rhizobium mutant is HH103 delta nuo, and is a mutant obtained by taking sinorhizobium fredii (sinorhizobium fredii) HH103 as a starting strain and performing gene knockout on a nuo control unit in an NADH oxidoreductase subunit I of the sinorhizobium fredii HH103. When the HH103 delta nuo is inoculated in the soybean seedling stage, the plant shows that the leaves are darker green, the plant biomass is up-regulated, the nitrogenase activity of a single plant is obviously higher than that of a wild type strain, and the plant height is integrally increased, so that the HH103 delta nuo improves the symbiotic effect of the rhizobium and the soybean.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The application provides a high-yield exocellular polysaccharide rhizobium LX and application thereof, and belongs to the technical field of biology, wherein the rhizobium LX is preserved in the China Center for Type Culture Collection, the preservation time is February 28, 2025, and the preservation number is CCTCC M 2025321; the rhizobium LX strain can efficiently synthesize exocellular polysaccharide in a conventional fermentation medium, the fermentation process has the characteristics of low cost, simple operation, short cycle and high yield (up to 30 g / L), and exhibits significant scale production advantages; the fermentation liquor or exocellular polysaccharide of the rhizobium LX can significantly enhance the ability of plants to resist saline-alkali and drought stress, and the required amount is less.

The present application relates to a kind of novel salt-tolerant rhizobium and its application, the rhizobium preservation number is GDMCC No:65918.The rhizobium of the application has host broad spectrum, can be symbiotic nodulation with leguminous plants including purple winged bean and peanut etc., improve the nitrogen fixation capacity of plant, and growth speed is faster.Especially, the rhizobium has high salt, acid, alkali tolerance, can grow in extreme salt, acid, alkali environment, improve soil, significantly promote the growth survival of plant in high salt, acid, alkali environment with symbiosis.The rhizobium of the application has very important application value to the development and utilization of leguminous plants, salt-alkali land (such as beach sand), ecological environment repair and improvement, and agricultural production.

The invention discloses a method for raising seedlings of cyperus rotundus, which comprises first evenly sowing cyperus rotundus seeds after germination in a culture matrix, then injecting a rhizobium strain GZMP‑08 bacterial solution into the culture matrix, keeping the culture matrix moist, and inoculating and culturing for 7d to 10d; transplanting the bud seedlings after inoculation and culturing into a container bag containing a seedling culture matrix; managing light and water during the seedling stage; fertilizing when the seedlings are transplanted for 3 months; performing root cutting treatment when the seedlings are transplanted for 4 months; and the cyperus rotundus seedlings can be planted out when the seedlings are transplanted for 5 months. The method for raising seedlings of the invention can obtain fast-growing high-quality seedlings with neat seedling emergence, thick seedling diameter, and developed taproots, and is resource-saving, environmentally friendly, and has simple and easy processes, and is a sustainable development approach that breaks through the traditional method for raising seedlings of cyperus rotundus.

The invention discloses a preparation method of a drought-resistant seedling-protecting rhizobium inoculant in the field of biological inoculant fertilizers. The method comprises the following steps: taking rhizobium, and performing fermentation culture to prepare a bacterial suspension; taking an organic carrier, doping the modified sea squirt cellulose nanocrystal dispersion liquid and a CaCl2 solution, and adsorbing the bacterial suspension on the organic carrier to obtain a bacterial material; the preparation method comprises the following steps: pretreating waste traditional Chinese medicine residues, performing oxygen-limited pyrolysis on part of the traditional Chinese medicine residues to obtain a carbon substance and pyrolysis condensate, mixing the carbon substance, the pyrolysis condensate and the remaining traditional Chinese medicine residues, and performing hydrothermal synthesis reaction to obtain a traditional Chinese medicine residue derivative carrier; adsorbing a liquid fertilizer on the traditional Chinese medicine residue derivative carrier to obtain a fertilizer; and uniformly mixing the fungus material and the fertilizer. Compared with a traditional liquid bacterial suspension, the rhizobium inoculant has the advantages that the problem that the rhizobium inoculant is permeated and lost after inoculation is avoided, the acting time of strains is prolonged, the contact symbiotic effect with root systems is improved, and the inoculation operation can be simplified; the liquid fertilizer can also assist in improving the drought resistance of the alfalfa seedlings and promoting the growth and development of the alfalfa seedlings.

The invention belongs to the technical field of microorganisms, and particularly relates to rhizobium exopolysaccharide and application thereof. By extracting and purifying the rhizobium extracellular polysaccharide and analyzing the yield and monosaccharide composition of the extracellular polysaccharide, the apoptosis action mechanism of the extracellular polysaccharide on the in-vitro liver cancer cell Hep-G2 is systematically researched. A mouse liver cancer model is constructed, a model group is used as a negative control, a specific drug group is used as a positive control, the liver cancer mouse model adopts concentration gradient exopolysaccharide intragastric administration as an experimental group, and the transcriptomics influence of the exopolysaccharide on liver cancer nude mouse blood and tumor samples is analyzed. The action effect of the exopolysaccharide in a Hep-G2 subcutaneous model is comprehensively characterized by various means such as liver and kidney toxicity test, Elisa experiment, HE staining and immunohistochemical experiment, and the regulation mechanism of the exopolysaccharide on nude mouse liver cancer tumor key protein is detected by adopting Western blot experiment.

The present application belongs to the field of microbial technology. More particularly, it relates to a salt-tolerant Bradyrhizobium sp. strain RY15 and its application. The Bradyrhizobium sp. strain RY15 provided by the present application has excellent salt tolerance and can significantly promote the growth and development and nodule fixation ability of Desmodium velutinum. The pot experiment results prove that the application of the RY15 strain can significantly increase the fresh weight of the aboveground part, the fresh weight of the underground part and the number of nodules of Desmodium velutinum; in the saline soil field, the yield of the pasture is increased by 68.89% after the inoculation of the RY15 inoculant. The RY15 strain of the present application can be made into a liquid or solid microbial inoculant, which is suitable for seed dressing, root soaking or soil application, and is simple to operate, low in cost and easy to popularize. The RY15 strain of the present application is a natural symbiotic bacterium, is safe and non-toxic, can improve the nitrogen cycle of soil, expand the planting of high-quality leguminous forage in saline-alkali soil, and has good application prospect and application value.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.