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Nutrient film protecting agent of rhizobium japonicum and production method thereof

A soybean rhizobia and production method technology are applied in the fields of agricultural microorganisms and biochemical industries, can solve the problems of inability to realize commercial production and application, inability to apply in large areas, attenuation of viable bacterial counts, etc., and achieve good field application effect and soybean root nodules The effect of high bacterial count and long shelf life

Inactive Publication Date: 2010-09-01
哈尔滨奥龙奇康科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The application of soybean rhizobia can be divided into three stages according to the dosage form of soybean rhizobia. The first stage is from the 1950s to the 1980s. The soybean rhizobia is a peat-adsorbed type with a shelf life of half a year. trouble
The second stage was after 1982, when liquid soybean rhizobia agents appeared, which were easy to apply, but the shelf life was too short, and they were easily spoiled, so they could not be applied on a large scale, and commercial production and application could not be realized.

Method used

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  • Nutrient film protecting agent of rhizobium japonicum and production method thereof
  • Nutrient film protecting agent of rhizobium japonicum and production method thereof
  • Nutrient film protecting agent of rhizobium japonicum and production method thereof

Examples

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Effect test

Embodiment 1

[0017] First, sodium methylcellulose, polyvinylpyrrolidone PVPK30 and polyvinylpyrrolidone PVP S630 were slowly stirred and dissolved in purified water respectively, and then the three were mixed together, then peptone, bovine serum albumin and sodium molybdate were added, at a certain Make it slowly dissolve and react under reaction conditions; the described parts by weight are contained in every 100 parts of soybean rhizobia nutrient film protective agent: polyvinylpyrrolidone PVPK30 is 1 part, polyvinylpyrrolidone PVPS630 is 1 part, 0.2 part of sodium methylcellulose, 5 parts of peptone, 0.01 part of bovine serum albumin, 0.1 part of sodium molybdate, and the rest are pure water; the reaction conditions are: the pH of the solution is 6.8, and the aeration ratio is 1 : 0.6, the temperature is 25° C. and the time is 50 hours, and the reaction vessel is a fermenter. The liquid is red in color, non-toxic and has a clear fragrance.

Embodiment 2

[0019] First, sodium methylcellulose, polyvinylpyrrolidone PVPK30 and polyvinylpyrrolidone PVP S630 were slowly stirred and dissolved in purified water respectively, and then the three were mixed together, then peptone, bovine serum albumin and sodium molybdate were added, at a certain Make it slowly dissolve and react under reaction conditions; the described parts by weight are contained in every 100 parts of soybean rhizobium nutrient film protective agent: 5 parts of polyvinylpyrrolidone PVPK30, 5 parts of polyvinylpyrrolidone PVPS630, 1 part of sodium methylcellulose, 10 parts of peptone, 0.1 part of bovine serum albumin, 0.3 part of sodium molybdate, and the rest are pure water; the reaction conditions are: the pH of the solution is 7.0, and the aeration ratio is 1 : 0.7, the temperature is 27° C. and the time is 55 hours, and the reaction vessel is a fermenter. The liquid is red in color, non-toxic and has a clear fragrance.

Embodiment 3

[0021] First, sodium methylcellulose, polyvinylpyrrolidone PVPK30 and polyvinylpyrrolidone PVP S630 were slowly stirred and dissolved in purified water respectively, and then the three were mixed together, then peptone, bovine serum albumin and sodium molybdate were added, at a certain Make it slowly dissolve and react under reaction conditions; the described parts by weight are contained in every 100 parts of soybean rhizobia nutrient film protective agent: 10 parts of polyvinylpyrrolidone PVPK30, 10 parts of polyvinylpyrrolidone PVPS630, 2 parts of sodium methylcellulose, 20 parts of peptone, 0.2 parts of bovine serum albumin, 0.5 parts of sodium molybdate, and the rest are pure water; the reaction conditions are: the pH of the solution is 7.2, and the aeration ratio is 1 : 0.8, the temperature is 30° C. and the time is 60 hours, and the reaction vessel is a fermenter. The liquid is red in color, non-toxic and has a clear fragrance.

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Abstract

The invention relates to a nutrient film protecting agent of rhizobium japonicum and a production method thereof. The nutrient film protecting agent of rhizobium japonicum is prepared by selecting the raw materials of PVP (Polyvinylpyrrolidone) K30, PVP S630, sodium carboxymethyl cellulose, peptone, bovine serum albumin and sodium molybdate and reacting under a certain reaction condition; the nutrient film protecting agent of rhizobium japonicum, a liquid rhizobium japonicum agent and soybean seeds are mixed and stirred according to a certain proportion; and after the soybean seeds are coated and stored for two days, each soybean seed can ensure 100,000 live rhizobium japonicums. The invention has wide needed raw material sources, simple production process and low cost, can play roles of nutrition, film formation and protection in the rhizobium japonicum, and can ensure that the liquid rhizobium japonicum agent is preserved for one year at normal temperature; the live bacterium quantity is not smaller than 2,000,000,000 / ml, and the nitrogen fixing activity is not reduced.

Description

Technical field: [0001] The invention relates to the technical fields of agricultural microorganisms and biochemical industry, in particular to a soybean rhizobium nutrient film protective agent and a production method thereof. Background technique: [0002] The research on soybean rhizobia in my country can be traced back to the 1930s. Professor Zhang Xianwu, a well-known expert in soil microbiology in my country, published the first article on the research and application of soybean rhizobia in 1937. It has a history of more than 80 years. After the founding of the People's Republic of China in 1950, Zhang Xianwu, Xu Guanghui, Ding Jian and others collected a total of 849 samples from Heilongjiang, Jilin, Liaoning and other places in Northeast China, isolated more than 500 soybean rhizobia species, and carried out artificial inoculation. In 1953, soybean rhizobia inoculant technology was promoted on 1.5 million hectares of soybean cultivation in Northeast China, increasing...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/41
Inventor 逄镜萍张菁
Owner 哈尔滨奥龙奇康科技发展有限公司
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