Special bacterial fertilizer for sainfoin and preparation method of special bacterial fertilizer
A technology for red bean grass and bacterial fertilizer, applied in the field of microorganisms, can solve the problems of reduced use efficiency of chemical fertilizers, high production costs, ecological pollution, etc., and achieve the effects of less consumption of non-renewable energy, low cost, and promotion of absorption
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Embodiment 1
[0025] In order to describe in detail the technical content, objectives and effects of the present invention, the following will be described in conjunction with the embodiments.
[0026] A special bacterial fertilizer for red bean grass, comprising: strain Rhizobium yanglingense GAU-00008, strain Bacillus sp. CHO87, Bacillus subtilis LSH11 and a carrier.
[0027] It should be noted that rhizobium is a kind of Gram-negative bacteria that exists in soil and can infect the roots of legumes and form root nodules. It almost only forms a symbiotic system with legumes. The strain Rhizobia yangling has a high The nodulation rate and the high nitrogenase activity of the root nodules; Bacillus has a high ability to dissolve inorganic phosphorus and organic phosphorus; Bacillus subtilis has strong and extensive pathogenic fungi for common red bean grass-soil-borne diseases biological protection characteristics.
[0028] Further, the carrier includes: charcoal powder, flower soil and hu...
Embodiment 2
[0034] A kind of preparation method of red bean grass special bacterial fertilizer, see figure 1 , the method includes the following steps:
[0035] Step (1), preparing the carrier: uniformly mixing charcoal powder, floral soil and humic acid to obtain the carrier, and then sterilizing the carrier.
[0036] Step (2), selecting strains: selecting the strain Rhizobium yanglingense GAU-00008, the strain Bacillus sp. CHO87 and the strain Bacillus subtilis LSH11.
[0037] Step (3), preparation of mixed bacterial liquid: bacterial strain Rhizobium yanglingense (Rhizobium yanglingense) GAU-00008, bacterial strain Bacillus sp. CHO87 and Bacillus subtilis (Bacillus subtilis) LSH11 were carried out liquid culture, and mixed, obtained Mixed bacteria.
[0038] It should be noted that after mixing, the measurement of the composite strain system showed that there was no antagonistic reaction among the three strains, and the strains maintained the original growth-promoting properties.
[...
Embodiment 3
[0041] A preparation method of special bacterial fertilizer for red bean grass, combining figure 2 , for the above figure 1 The preparation method of the shown red bean grass special bacterial fertilizer is discussed in detail.
[0042] Step (101): Mix the quality of charcoal powder, flower soil and humic acid according to the ratio of 2:1:1 to obtain a carrier, and the pH of the carrier after mixing is 7.0±0.2, and then carry out Autoclave sterilization (103.4KPa, 121°C, 30 minutes) or gamma-ray sterilization.
[0043] It should be noted that high-pressure steam sterilization, high temperature and high pressure sterilization, can kill general bacteria, fungi and other microorganisms, and is the most reliable and widely used physical sterilization method. Gamma-ray sterilization is a method that utilizes the characteristics of radiation to destroy the structure of bacteria to kill bacteria. In the embodiment of the present invention, the carrier is sterilized by high-press...
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