This invention belongs to the field of microbial technology and discloses a synthetic microbial
community for enhancing biological
nitrogen fixation in
karst grasslands, its construction method, and its application. The synthetic microbial
community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797;
Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous
forage grasses that require repeated
sowing, the seeds are treated with a synthetic microbial
community before being sown in the field; for perennial leguminous
forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous
forage grasses. This invention screens out suitable and efficient
root nodule endophytic bacteria in
karst areas and constructs a synthetic microbial community to enhance the biological
nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological
nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of
nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.