32 results about "Clonostachys rosea" patented technology

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

The invention provides clonostachys rosea-bacillus subtilis composite wettable powder containing clonostachys rosea fungus conidial powder for preventing and treating gray mold, sheath blight, root rot, fungal leaf spot and sclerotinia rot and bacillus subtilis bacterial spore powder for preventing and treating bacterial wilt, soft rot diseases, canker, bacterial spot diseases and bacterial angular leaf spot diseases. The wettable powder comprises, by weight, 50-100 parts of clonostachys rosea fungus conidial powder, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of sodium dodecyl benzene sulfonate, 10-50 parts of sodium lignosulfonate, 10-50 parts of carboxymethylcellulose sodium and 5-10 parts of ascorbic acid. The wettable powder composite microbes do not produce antagonistic action, have a wide sterilization spectrum, are safe for crops, users and an environment, improve crop quality and improve a yield and performance stability.

The invention relates to a clonostachys rosea (clonostachys rosea) strain YZC3 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the clonostachys rosea strain YZC3 is CGMCC No.14782. Based on the clonostachys rosea strain YZC3, the invention further provides a product capable of degrading lignin, a microbial agent for degrading the lignin,a product for composting park afforesting waste, a microbial agent for composting the park afforesting waste and a method for composting the park afforesting waste. The clonostachys rosea strain YZC3disclosed by the invention has excellent effects in the respect of degrading the lignin.

The invention discloses a continuous cropping-resistant compound microbial preparation and a preparing method thereof. The continuous cropping-resistant compound microbial preparation is a plant compound microbial agent. An active ingredient of the plant compound microbial agent is composed of streptomyces silaceus and clonostachys rosea. The streptomyces silaceus can be streptomyces silaceus SQ11, and the clonostachys rosea can be clonostachys rosea GR02. The continuous cropping-resistant compound microbial preparation can be prepared by a solid fermentation process, is simple and convenient,does not produce waste water and waste gas, has the advantages of pollution-free production, pure microbial microbial agent, and no addition of any hormones or chemical agents, guarantees the safetyof crop quality and zero pollution to the environment, reduces the production cost of farmers, guarantees the safe quality of agricultural products, and effectively reduces the amount of chemical fertilizers and pesticides.

The invention discloses a preparation method of biocontrol bacterium immobilized microspheres optimized by a response surface methodology, and belongs to the field of biocontrol bacterium agents. The invention aims to optimize the preparation process of the immobilized bacterium microspheres, and finds the optimal process conditions based on the response surface methodology optimization, so that the effectiveness of the immobilized bacterium microspheres in practical application is improved. The method comprises the following steps of transferring a Clonostachys rosea strain into PDA culture under a sterile condition, transferring the strain subjected to purification culture into a PDB liquid culture medium added with antibiotics, placing the PDB liquid culture medium in a constant-temperature shaking table for shaking culture to obtain Clonostachys rosea fermentation liquor, adding a sodium alginate solution, slowly dropwise adding into a sterilized CaCl2 solution by using an injector, and performing hardening to form the Clonostachys rosea immobilized microspheres. The influence of different conditions on the Clonostachys rosea embedding rate in the embedding process is explored by utilizing the response surface methodology, a continuous variable curved surface model is established, and the optimal embedding condition is fitted and determined through a regression equation, so that the maximum efficacy of microorganisms is exerted.

The invention discloses a method for producing podophyllotoxin by using callus adventitious roots of sinopodophyllum hexandrum royle. The method comprises the steps of obtaining sterile embryos, carrying out embryonic callus induction, adventitious roots growing, induction production of the podophyllotoxin, and the like. The set of method for producing the podophyllotoxin by coupling cell engineering with fermentation engineering is established. The method provided by the invention is simple to operate and high in yield, subculture does not occure to the calluses, browning degree is low; the adventitious roots are treated by using endophyte of the sinopodophyllum hexandrum royle, clonostachys rosea (fhnzm) fermentation liquor and some plant growth regulators, so that the content of the sinopodophyllum hexandrum royle is effectively increased.

The present invention discloses a clonostachys rosea engineering strain for transforming chitinase gene, wherein the strain is named Cr-chi32 and has the preservation number of CGMCC No.10027 in the China General Microbiological Culture Collection Center. The engineering strain Cr-chi32 has effects of enhancing of disease resistance of clonostachys rosea and improvement of prevention effect stability, can be effectively used for prevention and control of plant diseases, particularly for prevention and control of Sclerotinia and Botrytis cinerea of crops, and further provides good prevention and control effects on wilt, root rot, and other plant fungal diseases.

The invention provides a mutant of zearalenone-degrading enzyme with improved enzyme activity, its coding gene and application. The present invention uses the error-prone PCR method to Clonostachys rosea The source zearalenone degrading enzyme gene is modified, and then the zearalenone degrading enzyme mutants YMDS01, YMDS02 and YMDS03 are obtained through screening. The zearalenone-degrading enzyme mutant provided by the invention has significantly improved ability to degrade zearalenone, and has good market application prospects and industrial value.

The invention provides clonostachys rosea-bacillus subtilis composite wettable powder containing clonostachys rosea fungus conidial powder for preventing and treating gray mold, sheath blight, root rot, fungal leaf spot and sclerotinia rot and bacillus subtilis bacterial spore powder for preventing and treating bacterial wilt, soft rot diseases, canker, bacterial spot diseases and bacterial angular leaf spot diseases. The wettable powder comprises, by weight, 50-100 parts of clonostachys rosea fungus conidial powder, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of sodium dodecyl benzene sulfonate, 10-50 parts of sodium lignosulfonate, 10-50 parts of carboxymethylcellulose sodium and 5-10 parts of ascorbic acid. The wettable powder composite microbes do not produce antagonistic action, have a wide sterilization spectrum, are safe for crops, users and an environment, improve crop quality and improve a yield and performance stability.

The invention discloses a mildew Mycoplasma pink, its solid fermentation inoculant and its application, and aims to solve the technical problems of chemical pollution control, complicated biocontrol bacteria screening and complicated production process. Screening a strain of Slime sclerotiorum ( Clonostachys rosea ) NF‑06, whose deposit number is CGMCC No.16262. Culturing and preparing a solid fermentation inoculum obtained by solid fermentation of Mycoplasma pink strain NF-06. The Myxoplasma pink strain NF-06 or the solid fermentative inoculum is used in the control of plant root-knot nematode disease. The method of the invention is simple and feasible, low in cost, high in effective viable bacteria count of the spore powder, friendly to ecological safety, less chemical agent is used, simple in production process, low in cost, and beneficial for application and popularization.

The invention discloses a compound microbial preparation against heavy cropping and a preparation method thereof. The compound microorganism preparation against repeated cropping is a compound microorganism preparation for plants, and the active ingredients of the composite microorganism preparation for plants are composed of Streptomyces yellow ochreus and Clonospora pink. The Streptomyces silaceus may be Streptomyces silaceus SQ11, and the Clonostachys rosea may be Clonostachys rosea GR02. The anti-stubble compound microbial preparation of the present invention can be prepared by a solid fermentation process, which is simple and convenient, does not produce waste water, waste gas, and is produced without pollution. The pure microbial agent does not add any hormones and chemical preparations, ensuring crop quality safety and zero environmental impact. Pollution, reduce farmers' production costs, ensure the quality and safety of agricultural products, and effectively reduce the amount of chemical fertilizers and pesticides.

Clonostachys rosea strains have novel usefulness as inoculants of plants promoting plant vigor, health, growth, yield and a reduction of competitive stress caused by other fungi when used alone or sequentially with many fungicides in an integrated pest management system (IPM). Seed and foliar uses are shown to inoculate and subsequently achieve endophytic colonization of the portion of the plant treated. While the germinating conidia of this organism has been shown to tolerate several fungicide groups, the established mycelium of Clonostachys rosea is significantly more tolerant to systemic fungicides facilitating use in seed and foliar applications. Seed treatment and colonization may occur at any time after harvest resulting in endophyte enhanced seed that ignores or is marginally altered by other fungal organisms and may be suitable for Feed, Food and Seed uses.

The invention "a Clonostachys rosea strain YZC3 and its application" belongs to the technical field of microorganisms. The preservation number of the Glioma pink strain YZC3 is CGMCC No.14782. Based on Glioma pink strain YZC3, the present invention also provides a lignin-degradable product, an inoculant for degrading lignin, a product for composting landscaping waste, and an inoculant for composting landscaping waste , and a method for composting landscaping waste. The Glioma rosea strain YZC3 of the present invention has an excellent effect in degrading lignin.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

The invention discloses an engineering strain-clonostachys rosea for perilipin gene transfer and an application of the engineering strain. The engineering strain is named as Cr-per44, has a preservation registration number of CGMCC (China General Microbiological Culture Collection Center) No.10028 in the CGMCC, is obtained by transferring a parasitic related gene-perilipin gene Per3 into clonostachys rosea HLD-1 through protoplast transformation, is strong in disease resistance and stable in prevention effect, can effectively prevent and control sclerotinia and gray mold of crops, and has relatively good prevention and control effects on various plant fungous diseases such as blight and root rot.