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Continuous cropping-resistant compound microbial preparation and preparing method thereof

A micro-ecological preparation and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problem of soil sustainable production capacity and fertilizer utilization rate reduction, continuous cropping obstacles, soil-borne diseases, etc. problems, to achieve the effect of ensuring the quality and safety of agricultural products, ensuring the quality and safety of crops, and reducing farmers' production costs

Active Publication Date: 2019-08-16
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Long-term use of chemical fertilizers, chemical pesticides, and high-intensity multiple cropping and continuous cropping farming methods will lead to increased resistance to diseases and insect pests in the agricultural production process, reduced soil sustainable production capacity and fertilizer use efficiency, and damage to the quality of cultivated land. Insufficient organic matter, Soil compaction, decline in fertility, frequent occurrence of soil-borne diseases and continuous cropping obstacles, and increasingly serious problems in the quality and safety of agricultural products have restricted the development of my country's agriculture.

Method used

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  • Continuous cropping-resistant compound microbial preparation and preparing method thereof
  • Continuous cropping-resistant compound microbial preparation and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment 1, the isolation and identification of Clonosporium pinkhelix strain

[0092] 1. Strain isolation

[0093] Separation from the root soil of Dayekou trees in Qilianshan National Nature Reserve, Gansu Province, China, using the dilution plate method, the specific operation method is as follows: Weigh 10 g of soil samples, pour them into a triangular flask filled with small glass beads and 90 ml of sterile water, shake After 30 minutes, let it stand for 5 minutes, and then dilute 10 times successively to make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Take 0.1ml of suspensions of different concentrations and add them to Gao’s No. 1 medium plate, evenly spread them and place them at 28°C for observation. After 5-7 days, pick different single colonies to streak and purify. The purified strains were transferred to Gao's No. 1 slant medium for culture, and stored at 4°C for future use. The strain numbered GR02 (strain GR02) was taken for the following identification. ...

Embodiment 2

[0098] Embodiment 2, the preparation of probiotics for plants

[0099] 1. Preparation of Streptomyces yellow ocher SQ11 microecological preparation

[0100] Pick up Streptomyces chrysogenum SQ11 grown on the plate with an inoculation loop and inoculate it into a 500mL Erlenmeyer flask filled with 100mL of PDA medium, shake at 28°C, 26mm amplitude, 170r / min for 24 hours to obtain Streptomyces ochraceus SQ11 seed culture liquid; the Streptomyces ochreus SQ11 seed culture liquid is transferred to the 500ml Erlenmeyer flask that 50g bacterial fermentation medium is housed by the inoculum size of 10% (v / v), and 28 ℃ are cultivated for 5 days, collect in the culture container All substances were obtained from Streptomyces chrysanthemum SQ11 probiotics (ie all substances in the culture container). In the Streptomyces ochraceus SQ11 probiotics, the content of Streptomyces ochraceae SQ11 is 4×10 9 cfu / g.

[0101] 2. Preparation of Microecological Preparation of Clonosporium pinkheli...

Embodiment 3

[0105] Embodiment 3, the probiotics for plants of the present invention promote the growth of watermelon seedlings

[0106] 1. Preparation of cultivation substrate

[0107] 1.1 Preparation of blank cultivation soil

[0108] Vermiculite and nutrient soil were mixed according to the volume ratio of 1:1 to obtain blank cultivation soil.

[0109] 1.2 Cultivation soil of Streptomyces yellow ocher SQ11

[0110] The blank cultivation soil of 1.1 was mixed with the Streptomyces ochreus SQ11 probiotics of Example 2 to obtain the SQ11 cultivation soil of Streptomyces yellow ochrescens. The content of Streptomyces yellow ocher SQ11 in the cultivation soil of Streptomyces yellow ocher SQ11 was 4×10 8 cfu / g.

[0111] 1.3 Cultivation soil of Clonosporium GR02

[0112] The blank cultivation soil of 1.1 was mixed with the Clonospora rosea GR02 probiotics of Example 2 to obtain the Clonospora rosea GR02 cultivation soil. The content of Clonosporium GR02 in the clonosporium GR02 cultivati...

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Abstract

The invention discloses a continuous cropping-resistant compound microbial preparation and a preparing method thereof. The continuous cropping-resistant compound microbial preparation is a plant compound microbial agent. An active ingredient of the plant compound microbial agent is composed of streptomyces silaceus and clonostachys rosea. The streptomyces silaceus can be streptomyces silaceus SQ11, and the clonostachys rosea can be clonostachys rosea GR02. The continuous cropping-resistant compound microbial preparation can be prepared by a solid fermentation process, is simple and convenient,does not produce waste water and waste gas, has the advantages of pollution-free production, pure microbial microbial agent, and no addition of any hormones or chemical agents, guarantees the safetyof crop quality and zero pollution to the environment, reduces the production cost of farmers, guarantees the safe quality of agricultural products, and effectively reduces the amount of chemical fertilizers and pesticides.

Description

technical field [0001] The invention relates to a compound microbial preparation against heavy cropping and a preparation method thereof. Background technique [0002] Long-term use of chemical fertilizers, chemical pesticides, and high-intensity multiple cropping and continuous cropping farming methods will lead to increased resistance to diseases and insect pests in the agricultural production process, reduced soil sustainable production capacity and fertilizer use efficiency, and damage to the quality of cultivated land. Insufficient organic matter, Soil compaction, decline in fertility, frequent occurrence of soil-borne diseases and continuous cropping obstacles, and increasingly serious problems in the quality and safety of agricultural products have restricted the development of my country's agriculture. The microbial fertilizers and microbial pesticides prepared by microbial strains with bio-control functions through biological fermentation technology and preparation p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P3/00A01P21/00C12R1/465C12R1/645
CPCC12N1/20C12N1/14A01N63/00A01N63/30Y02A40/10
Inventor 吴慧玲刘伟成苏道拉呼圆圆张涛涛刘霆董丹赵娟田兆丰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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