44results about How to "Promote stable production" patented technology

This invention belongs to the molten slag treatment, especially relates to the blast furnace molten slag treating apparatus. The slag ditch connects with the granulating device and slag flume, the down part of the slag flume connects with the communicating pipe, the distributing trough that connects with the communicating pipe extends into the rotating drum filter, the belt device in the canister connects with the rotating belt device to form a finished articles warehouse; the down part of the rotating filter is arranged with the storage cabinet, laying-up basin, which are connected by the overflow on the pool wall, the dregs cleaning device is arranged on the storage cabinet the laying-up basin connects with the granulating device by the tube connector, valve and dregs cleaning pump. The device fault rate is low, the dregs punching out rate reaches up to more than 99úÑ, which is propitious to the operation and steady production of the blast furnace. And the dreg cleaning water and the sewage is used in closed cycle, so it can save a lot of pure water.

The invention provides a method for quickly desalting, dealkalizing and ploughing a soda saline-alkali paddy field. The method comprises the following steps of: in the rice field after rice harvest in autumn, turning up the soil by using a big plough in autumn after base fertilizer is applied; sunning the upturned soil; rotating the soil in a dry mode in spring of the next year; ridging and soaking; leveling the field by using a thick rake until slurry is settled; and transplanting. In the method, spring soil turnup is replaced by autumn soil turnup; autumn soil turnup is combined with springsoil rotation; advantages of two tillage methods of rotary tillage and autumn soil turnup are utilized, advantages are absorbed and disadvantages are complemented, advantages are utilized and disadvantages are avoided, soil fertility is adjusted, and soil application and improvement are taken into consideration; and water resource is saved, saline and alkali are removed quickly, the soil structure is optimized, fragile ecological environment is improved, high yield, stable production and sustained development of rice in saline-alkali adverse situation are promoted. Rice is planted in the sodasaline-alkali land by the method, so the method is simple and easy in operation and convenient to popularize, labor and time are saved, production input cost is low, yield is increased obviously, repair of the fragile ecological environment is facilitated, and income of farmers is increased.

The invention discloses a fracturing method for improving complexity of deep shale gas cracks. According to the fracturing method for improving the complexity of the deep shale gas cracks, the mode ofa prepositioned high-viscosity glue solution and low-viscosity quick water and a high-viscosity glue solution is adopted, propping agents of 140-230 meshes and 70-140 meshes are used for filling andsaturating the cracks with various sizes, and the complexity of the deep shale gas fracturing cracks is improved and the effective transforming volume is increased. According to the fracturing methodfor improving the complexity of the deep shale gas cracks, liquid performance parameters, crack parameters and corresponding construction parameters are determined by using fracturing earlier stage optimal design, the mode of the prepositioned high-viscosity glue solution and the low-viscosity quick water and the high-viscosity glue solution is adopted, an optimization rapid and synchronous gel breaking technology is used, the propping agents of the 140-230 meshes and 70-140 meshes are used, finally, the complexity of the deep shale gas fracturing cracks is improved and the effective transforming volume is increased, and increased production and stable yield of a shale gas well are facilitated.

The invention relates to a simple and labor saving fertilization method for summer maize under a wheat and maize rotation mode. The method comprises the steps: harvesting wheat, then, smashing strawsto be completely returned to the field, next, sowing maize, and applying elementary substance fertilizers including a nitrogen fertilizer, a phosphorus fertilizer and a elementary fertilizer at one time during sowing maize, wherein topdressing is not needed in the whole growth period. The nitrogen fertilizer is a mixture of coated urea and ordinary urea, wherein the mass ratio of the coated urea is 60-80%, and the mass ratio of the ordinary urea is 20-40%. The fertilizers applied in the method are the elementary substance fertilizers by which the demands for nutrients in the whole growth period of maize can be met and topdressing is not needed in the whole process, so that the labor cost is reduced, the utilization ratio of the nitrogen fertilizer is greatly increased, the leaching loss ofthe nutrients along with rainwater is reduced, the nutrient supply during stem elongation and booting of maize is guaranteed, the yield of maize is greatly increased, stable production and yield increment is favorably realized, and meanwhile, the investment cost is greatly reduced.

The invention provides prefabricated plate type seedbed soil and a preparation method for raising paddy rice seedlings. The preparation method comprises the following steps of: smashing rice hull, blighted grains and straw, mixing according to a certain proportion, adding an EM (Effective Microorganism) microbial agent and water for stockpiling and storing, fully composting, and fermenting to obtain a fermentation mixture; uniformly mixing and stirring the fermentation mixture, perlite, grass carbon, humic acid, a hydrophilizing agent and a multifunctional paddy rice seedling raising conditioner according to the certain proportion, spraying clear water till raw materials are not loosened when extruded into sheets, and stirring uniformly; and extruding into sheets, splicing and drying to obtain the prefabricated plate type seedbed soil. According to the invention, the aims of saving the soil resource, efficiently utilizing resources and maintaining and repairing the weak ecological environment can be fulfilled, high yield, stable yield and sustainable development of paddy rice in cold paddy rice regions, saline and alkaline paddy rice regions and the like are facilitated, and the social benefit, economic benefit and ecological benefit are enhanced.

The invention discloses a slim hole explosion crack generation assisted hydraulic fracturing method. The method includes the following steps: firstly, windowing is carried out to side-drill a horizontal drilling well to form a long open slim hole, an explosive is put, and after the explosive explodes to impact rock, a large number of microcracks and micro-gaps are formed; secondly, perforation operation is carried out on the crack growing gaps formed by the impact of explosion pulse, slick-water is injected into perforation holes to form a main crack, the radial cracks and activated natural cracks are further opened to communicate with the main crack to form a complex crack network of main crack-branch crack, a glue solution or slick-water fracturing fluid mixed with a proppant is pumped for sanding to further extend and expand the main crack and the explosion cracks, the cracks are filled with the proppant, continuous sanding is carried out until designed sand volume is reached and then the operation is stopped. The method of the invention is simple in operation and mature in technology and can effectively improve the transformation efficiency of a deep shale gas reservoir.

The invention provides bacillus velezensis MES861, and an application and product thereof, and relates to the technical field of microorganisms. The bacillus velezensis MES861 is preserved in China General Microbiological Culture Collection Center on Dec.3,2018, and the preservation number is CGMCC NO.16857. The strain has an obvious antagonistic effect on fusarium graminearum, can prevent or reduce occurrence of rice seedling blight, promote stable yield, increase income, and improve and increase the yield and quality of rice.

The invention discloses a potato fertility improving method for carrying out straw field changing and returning on newly-prepared farmland. The potato fertility improving method includes the steps of selecting the newly-prepared farmland to carry out straw field changing and returning to plant potatoes, rapidly improving the fertility of soil of the newly-prepared farmland through straw and potato straw, deeply plowing the newly-prepared farmland in the mid-to-late September to enable the thickness of the plowed layer to be larger than 25 cm, ditching furrows according to the potato planting technology, leveling the farmland, applying base fertilizers which include, by mass, 10 kg-15 kg per mu of urea, 30 kg-50 kg per mu of calcium magnesium phosphate fertilizers and 40 kg-50 kg per mu of potassium sulfate at a time, planting the potatoes with the standard of 5000 holes-6000 holes per mu, covering ridges with prepared straw with the standard of 400 kg-600 kg per mu, conventionally managing the planted potatoes, harvesting the potatoes in the mid-to-late April next year, and carrying out cyclic operation of farming work such as preparing next-stubble crop planting and the like. The potato fertility improving method is high in economic benefit, after the potatoes are harvested, the potato straw and the decayed straw serve as organic materials to be directly returned to the farmland, and the good effects on the rapid fertility improvement and the physical and chemical property improvement of the soil of the newly-prepared farmland are achieved.

The invention relates to a camellia oleifera cultivation and pruning method. The method comprises four steps of digging holes and beginning to grow, fertilizing, planting, and trimming and pruning, wherein the process of trimming and pruning involves main trunk determining in early stage, and main branch pruning; in the second year of the plantation, the tree seedling is treated with the main trunk determining in a height of 25cm- 30cm; in the third year of the plantation, the tree seedling is treated with the main trunk determining in a height of 30cm- 40cm; the main branch of the camellia oleifera is treated by allowing the portion with green leaves to be exposed in soil, and removing the central branches, that is a treatment like opening a skylight, and the central branches of the maintrunk and side branches uniformly axially distributed around the periphery of the main brunch are pruned, thereby exposing the branches of an inner layer; three to four exuberant side branches are selected and reserved to be cultivated as main branches; and for each layer of main branches, three to four main branch buds in different directions are selected and reserved to form into side branches,and gradually form a good-shaped tree with a lower tree height and an opening tree crown. According to the camellia oleifera cultivation and pruning method, the process of camellia oleifera cultivation and pruning is improved, close planting and brachysm are carried out, the fertilizer, water and nutrition are reserved, thereby improving the survival rate and fruiting rate of camellia oleifera toachieve stable yield and high yield.

The invention discloses a cold-injury-resistance gene of lycopersicon esculentum mill and an application. On the basis of virus induced gene silencing (VIGS) technology, the novel chilling-injury-resistance related gene SISLD of the lycopersicon esculentum mill is discovered, and the gene, through silencing, can result in obvious decrease in low temperature tolerance ability of a tomato plant. Based upon analysis on an amino acid sequence encoded by the gene, protein encoded by the gene is [delta]8-sphingomyelin dehydrogenase, which is related to lipid metabolism of neurolemma, and the enzyme (the [delta]8-sphingomyelin dehydrogenase) plays an important role in a reaction that a plant resists to low-temperature stress. According to the research, a nucleotide sequence and an amino acid sequence of the gene are provided; and meanwhile, the invention also relates the application of the gene in cold-resistance breeding of tomato. The SISLD gene, when applied to anti-freezing breeding of the tomato, can improve the adaptability of tomato's high-quality varieties, extend a planting range of the tomato's high-quality varieties and promote stable production and high-yield production of the tomato's high-quality varieties.

The invention relates to the technical field of agricultural production, in particular to a method for planting corn in red-soil upland by water and fertilizer integration; the method comprises: applying base fertilizer, seedling fertilizer, jointing fertilizer and earing fertilizer to each of corn seeding stage, seedling stage, jointing stage and tasseling stage by means of water-fertilizer integration; total fertilizer for application includes 14-16 kg of nitrogen fertilizer by N per mu, 5-7 kg of phosphorus fertilizer by P2O5 per mu, and 11-13 kg of potassium fertilizer by K2O per mu. The method has the advantages that water loss and nutrition leaching loss can be decreased, conversion and absorption of fertilizer can be promoted, fertilizer utilization rate can be increased, water utilization can also be adjusted, and water utilization rate is increased.

The invention discloses a medicine for preventing fish parasitic crustacean diseases, a preparation method and an application thereof. The method comprises the following steps that pyridaben with mass percentage of between 5 and 30 percent and a surface active agent with mass percentage of between 5 and 25 percent are mixed; after the mixture is stirred for 25 to 35 minutes at a temperature of between 25 and 35 DEG C under 280-320rpm, a 45 to 90 percent solvent is added into the mixture to be stirred for 25 to 35 minutes to prepare the medicine for preventing the fish parasitic crustacean diseases. The method successfully applies pyridaben to the preparation of the medicine for preventing fish parasitic crustacean diseases for the first time. The medicine for preventing fish parasitic crustacean diseases has simple preparation process and easy operation, good effect, small dosage, long lasting period, safe for fish and non-pollution to ecological environment.

The invention relates to preparation of a fertilizer, in particular to an organic-inorganic compound fertilizer and a preparation method thereof, and belongs to the technical field of fertilizer processing. The method comprises the following steps of: fermenting and deodorizing livestock excrement, rice husk, straw and the like; mixing inorganic fertilizers such as urea, ammonium phosphate, potassium chloride, potassium sulfate and the like; and granulating and drying to obtain a finished product. The compound fertilizer has a reasonable formula and a simple processing technique, contains complete nutrients, can be used for effectively improving soil hardening and the like, and helps to facilitate stable production and high yield of agriculture.

The invention relates to the technical field of geophysics, in particular to a fracturing method for a complex seam of a dense sandstone gas reservoir. The fracturing method solves the technical problems that a fracture formed by conventional fracturing shows the sand-levee-type non-uniform laying situation, communicating of the reservoir matrix space cannot be better achieved, after transformation, the yield often does not meet the expected requirements, and the stable yield difficulty is large. The fracturing method comprises the steps that in the stage of prepad fluid, fracturing fluid is injected into a formation; in the stage of sand-carrying fluid, the fracturing fluid continues to be injected into the formation, and meanwhile, a proppant is continuously pumped; and in the stage of displacement fluid, the fracturing fluid is fully injected into a wellbore. The fracturing method for the complex seam of the dense sandstone gas reservoir at least has the following advantages that through the fracturing method, the finally-formed fractures are evenly laid, communicating of the reservoir matrix space can be better achieved, the yield is increased after transformation, the expectedrequirements are met, and the stable yield is facilitated.

The invention relates to a cultivation method for intercropping and interplanting agaricus bisporus in corn fields, and belongs to the technical field of cultivation of crops. The cultivation method comprises the following steps of plowing soil, performing ridging, planting the corn and the agaricus bisporus in a manner that wide ridges and narrow ridges are in alternative arrangement, wherein the ridge width of the corn is 30cm-60cm, and the ridge width of the agaricus bisporus and the ridge width of an empty ridge are both 70cm-100cm; planting two rows of the corn on each ridge of the corn ridges, wherein the ridge spacing of two rows of the corn on the same ridge is 25cm, and the planting spacing of two adjacent corn plants on the same row is 25cm-27cm; after 2-3 months that the corn is sown, laying a cultivation material on the ridges of the agaricus bisporus, uniformly sowing agaricus bisporus bacteria species in a spreading manner, and performing earthing up; and according to the growth requirements of the corn and the agaricus bisporus outdoors, performing culturing, and performing harvesting when the corn and the agaricus bisporus are mature. The method provided by the invention realizes the intercropping and interplanting of the corn and the agaricus bisporus, so that the economic benefits are very obvious, and besides, for each square meter of the soil, 20kg of corn straw and livestock and poultry dung are used, so that the condition of environmental pollution caused by the corn straw and the livestock and poultry dung is greatly relieved.

The invention discloses a cultivation method of tung tree, which includes site preparation, seed selection and seedling cultivation, young forest tending, transplanting, shaping and pruning, and pest control. The cultivation technology of the invention has simple steps, rapid seedling cultivation, can rationally utilize land, and improve land utilization rate, increase income, and reduce investment in tung tree management, which can promote premature birth, high yield and stable production of tung tree.

The invention discloses a prediction method of coke thermal performance. The prediction method comprises the following steps of (1) determining a coal blending coking microstructure according to a coal blending structure; (2) determining behavioral indicators of a coking process, performing weighted average on the fluidity, which is larger than or equal to 1000 to 10000 ddpm, of coking coal, recording lgMF1, performing weighted average on the fluidity, which is larger than or equal to 10000 ddpm, of coking coal, recording lgMF2, performing weighted average on the expansion degree of coking coal of which a b value is larger than or equal to 80%, and recording b; (3) solving a correlation coefficient relationship of all control indicators and the thermal performance: CSR=K+K1*C(Coarse)+K2*F(Fiber)+flaky+K3*T homogeneity+K4*X1lg MF1+K5*X2 lg MF2+K6*X2b; (4) determining control ranges of all of the indicators which reach the CSR requirements, and adjusting specific matching of blended coal. The prediction method is favorable for stable production of a blast furnace and can be widely applied to the field of coal chemical industry, and the coke thermal performance CSR reaches production requirements.

The invention belongs to the technical field of oil field oil extraction, and particularly provides a method for quickly removing resistance and blockage of a soluble bridge plug. The method comprisesthe following steps: sequentially injecting clear water, an accelerated dissolving solution slug and clear water into a wellhead, and stopping a pump and closing a well after the slug reaches a blockage position; injecting clear water and stopping the pump and closing the well after the slug is displaced to pass through the resistance position; injecting a sand-carrying fluid slug into the wellhead to polish the wall of the shaft sleeve so that a bridge plug rubber sleeve and dissolved residues on the wall of the sleeve are peeled off; and feeding soluble balls so that residues dispersed andretained in the wellbore are pushed to a toe fractured well section. The problems that the existing ignition release soluble bridge plug cannot be completely dissolved in a low-temperature stratum, thebridge plug rubber sleeve and residues are still located on the wall of the sleeve, normal pumping of a follow-up bridge shooting tool is affected, labor intensity is high, cost is high and risks arelarge can be solved. The soluble bridge plug is rapidly removed, the wellbore is smoothly cleaned and subsequent operation is smooth only through operation at the to-be-pressed wellhead without opening the wellhead or putting down grinding and milling tubular columns.

The invention discloses application of a sesamum indicum SiERF103 gene in enhancing plant resistance, and belongs to the technical field of plant genetic engineering, wherein a nucleotide sequence of the sesamum indicum SiERF103 gene is shown as SEQ ID NO.1, and the resistance comprises waterlogging resistance and / or drought resistance. The invention reports the application of the sesamum indicum SiERF103 gene in enhancing plant waterlogging resistance and / or drought resistance for the first time. By constructing an overexpression vector of the SiERF103 gene, the SiERF103 gene is overexpressed in arabidopsis thaliana, drought or waterlogging stress treatment is carried out, and research finds that normal growth conditions are recovered after the drought or waterlogging stress treatment, so that a large amount of transgenic arabidopsis thaliana can recover normal growth. The invention proves that the sesamum indicum SiERF103 gene has a function of improving plant waterlogging and drought resistance, which provides genetic resources for genetic improvement of crop drought resistance and waterlogging tolerance, and is of great significance for promoting crop stability.

The invention provides a degraded grassland reseeding ecological restoration method. Comprising the following steps: 1) selecting reseeding grass seeds; 2) applying a fertilizer; (3) no-tillage reseeding; and 4) management after sowing. According to the method, annual and perennial pasture, leguminous and gramineous pasture, deep-root and shallow-root pasture, photophilous pasture and lucifugal pasture are selected for matching and mixed sowing, the yield advantages of different pasture varieties in different succession stages and the complementarity of the pasture varieties in the aspect of water and nutrient utilization can be brought into full play, the high yield and stable yield of the improved degraded grassland are promoted, and the quality of the improved degraded grassland is improved. The nutritional value and quality of pasture are improved. No-tillage reseeding is adopted, the ploughing depth is only located on the surface layer of the soil, and along with completion of reseeding, the pressing process is followed, so that effective implantation of the seeds is guaranteed, and damage to native vegetation is reduced to the minimum. In addition, a straw mat is covered on a part of areas which are seriously degraded and small in native vegetation coverage, so that the soil moisture content is effectively improved, high-temperature damage of summer high temperature to reseeding grass seedlings is reduced, and the survival rate of the reseeding seedlings is remarkably increased.

The invention provides a method for quickly desalting, dealkalizing and ploughing a soda saline-alkali paddy field. The method comprises the following steps of: in the rice field after rice harvest in autumn, turning up the soil by using a big plough in autumn after base fertilizer is applied; sunning the upturned soil; rotating the soil in a dry mode in spring of the next year; ridging and soaking; leveling the field by using a thick rake until slurry is settled; and transplanting. In the method, spring soil turnup is replaced by autumn soil turnup; autumn soil turnup is combined with springsoil rotation; advantages of two tillage methods of rotary tillage and autumn soil turnup are utilized, advantages are absorbed and disadvantages are complemented, advantages are utilized and disadvantages are avoided, soil fertility is adjusted, and soil application and improvement are taken into consideration; and water resource is saved, saline and alkali are removed quickly, the soil structure is optimized, fragile ecological environment is improved, high yield, stable production and sustained development of rice in saline-alkali adverse situation are promoted. Rice is planted in the sodasaline-alkali land by the method, so the method is simple and easy in operation and convenient to popularize, labor and time are saved, production input cost is low, yield is increased obviously, repair of the fragile ecological environment is facilitated, and income of farmers is increased.

The invention provides a method for managing and controlling dwarfed coconut flowers and fruits, and relates to the technical field of plant flower and fruit management and control. The method comprises the following steps of: 1) arranging pollination trees in a dwarf coconut garden according to the quantity ratio of production trees to pollination trees (5-10) : 1; 2) applying a flowering agent to the pollination trees within 1-3 days after a spathe of the pollination trees is opened; the flowering agent is ethephon of 50-200 ppm or a naphthylacetic acid of 300-500 ppm ; 3) spraying an anti-dropping agent to the production trees after the pollination is finished; the anti-stripping agent is a mixed solution comprising 50-200 ppm of 2.4-D and 50-200 ppm of an indolebutyric acid. Accordingto the method for managing and controlling dwarfed coconut flowers and fruits, the pollination trees are configured, the male flower quantity is increased, and the female flower shedding is slowed down, so that the fruit setting quantity of the coconut is ensured, the flower and fruit productivity of the coconut is optimized, and the coconut flower and fruit configuration is controlled to promotethe high yield of the coconut, and a foundation is laid for the production and application of the dwarf coconut garden.

The invention discloses a medicine for preventing fish parasitic crustacean diseases, a preparation method and an application thereof. The method comprises the following steps that pyridaben with masspercentage of between 5 and 30 percent and a surface active agent with mass percentage of between 5 and 25 percent are mixed; after the mixture is stirred for 25 to 35 minutes at a temperature of between 25 and 35 DEG C under 280-320rpm, a 45 to 90 percent solvent is added into the mixture to be stirred for 25 to 35 minutes to prepare the medicine for preventing the fish parasitic crustacean diseases. The method successfully applies pyridaben to the preparation of the medicine for preventing fish parasitic crustacean diseases for the first time. The medicine for preventing fish parasitic crustacean diseases has simple preparation process and easy operation, good effect, small dosage, long lasting period, safe for fish and non-pollution to ecological environment.

The invention discloses a rice field milk vetch sowing-free circulation seed reserving method. The method comprises the following steps that after milk vetch seeds are mechanically harvested in early and mid May, smashed milk vetch straws are evenly spread in a rice field, irrigation is conducted, a drainage outlet is blocked (so as to prevent milk vetch seeds reserved in the rice field from flowing away along with water), biodegradation is conducted for five to ten days, dry farming is conducted to achieve single-season rice transplantation or single-season rice no-tillage direct sowing, the single-season rice is irrigated by shallow water at the early growth stage, water falling and field drying are conducted at the rice milk-ripe stage, the single-season rice is harvested from the mid and late September to early October, high stubbles of straws are reserved to be returned to the field, or removed from the seed reserving field within one to three days, ditching or ditch cleaning is conducted, and no water is reserved in the field in sunny days or accumulated in rainy days; routine management of milk vetch planting is conducted, and circulation operations of farming work such as milk vetch seed mechanical collection and single-season rice sowing are conducted in the next early and mid May. The rice field milk vetch sowing-free circulation seed reserving method solves the problems that at present, field abandonment is general in winter in the single-season rice field, and rice field green manure milk vetch seeds are scarce, and relieves milk vetch seed shortage, increases milk vetch seed reserve, and achieves the effect of returning milk vetch straws for fertilizing rice field soil.

The invention discloses a method for planting anoectochilus formosanus in the northern area. The method comprises the following steps: (1) woodland selection, (2) seedling selection, (3) seedbed preparation, wherein the northern area with the temperature in spring, summer and autumn ranging from 15 DEG C to 30 DEG C is selected as the cultivation field, soil under chestnut trees is selected, soil texture is required to be loose, breathable and good in moisture retention, the matrix on the ridge surface is formed by adopting and evenly stirring and mixing 1 / 2-2 / 3 of organic fertilizer and 1 / 3-1 / 2 of under-wood fine soil humus, before plantation, spraying and sterilizing are carried out through carbendazim or thiophanate, plastic films cover the matrix for 2-3 days and then are uncovered, and the matrix is prepared for use, (4) transplantation, (5) management after plantation, and (6) harvest. According to the method, a complete set of the technology for planting the anoectochilus formosanus under woods in the northern area is formed. The wild environment is imitated, local natural products are explored and utilized for mixing preparation to form the matrix for transplantation, and therefore the production cost is low; the cultivation technology for improving the plantation survival rate of the anoectochilus formosanus is formed, and therefore the stable yield is achieved easily; by the adoption of the method, the anoectochilus formosanus grows normally, and the net weight of a single plant is increased.

The invention discloses a method for determining the optimal nozzle size of a tight oil volume fracturing well, comprising the following steps: S1: obtaining basic data of a target well; S2: determining a reasonable nozzle size of the target well; S3: calculating different reasonable nozzle sizes Under the liquid production index and oil production index, the nozzle size corresponding to the maximum liquid production index and oil production index is the optimal nozzle size; S4: Calculate the declining production; S5: Calculate the cumulative liquid production under different reasonable nozzle sizes; S6 : Calculate the cumulative oil production under a single nozzle size; S7: Determine the size of the combination of nozzles; S8: Calculate the oil production index under different combination sizes of nozzles; S9: Calculate the cumulative oil production under different combination sizes of nozzles, cumulative oil production The combination size of the nozzle corresponding to the highest is the optimal combination size of the nozzle. The invention makes full use of the natural elastic energy of the oil reservoir, delays the decline period, makes more oil recovery and greater ultimate recovery ratio, and provides technical support for the efficient development of tight oil reservoirs.

The invention relates to Clonostachys rosea, Clonostachys rosea liquid and application of the Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary. The collection unit name of the Clonostachys rosea MES is China General Microbiological Culture Collection Center, and the collection number is CGMCC 3.11519. The Clonostachys rosea liquid contains 1.8 * 10<7> to 2.3 * 10<7> Clonostachys rosea spores per mL. The preparation method comprises the following steps of: picking fresh activated and cultured Clonostachys rosea hyphae, inoculating a plate containing a solid PDA (potato dextrose agar) culture medium with the fresh activated and cultured Clonostachys rosea hyphae, and culturing in a constant-temperature incubator at 25-30 DEG C for 12-16 days; and adding 20-30mL of Tween 80-containing sterile water into the culture dish, washing off the spores with a coater, filtering, and carrying out 8-12-time gradient dilution. The Clonostachys rosea liquid provided by the invention has obvious antagonistic effects on sclerotinia sclerotiorum causing Sclerotinia sclerotiorum(Lib.)deBary, and is capable of preventing or relieving the occurrence of Sclerotinia sclerotiorum(Lib.)deBary, promoting stable yield and income increase of sunflowers and improving the yield and quality of sunflowers.

The invention discloses a special fertilizer for the integration of water and fertilizer for corn and an application method thereof. The special fertilizer for the integration of water and fertilizer includes a special fertilizer for emergence, a special fertilizer for the ear stage, and a special fertilizer for the flowering and grain stage; the three special fertilizers all include the following components: Urea, diammonium phosphate, potassium chloride, zinc chelate, boric acid, manganese chelate, magnesium chelate, iron chelate, copper chelate. The special fertilizer for the integration of water and fertilizer for corn of the present invention is developed according to the demand law of corn for nutrients, adopts different fertilizer formulas in different growth stages of corn, and has strong pertinence; the ratio of nitrogen, phosphorus and potassium is reasonable, and meets the requirement of large amounts of nutrients in different growth and development stages of corn. At the same time, adding trace elements can play a role in balanced fertilization, higher fertilizer utilization efficiency, and can significantly increase corn yields to achieve the purpose of increasing production and income; all nutrients are quick-acting nutrients, good water solubility, stable properties, and drip irrigation Combined with the method, the dripper will not be blocked during the drip irrigation process, has the advantages of saving fertilizer and water, and is suitable for popularization and application.

Relating to an Alpha subunit holoprotein gene of phycobiliprotein from Spirulina and an encoding protein thereof as well as a recombinant vector containing the Alpha subunit holoprotein gene of phycobiliprotein from Spirulina, the present invention provides an Alpha subunit holoprotein gene of phycobiliprotein from Spirulina, an encoding Alpha subunit holoprotein of phycobiliprotein from Spirulina and an amino acid sequence thereof as well as a recombinant vector containing the Alpha subunit holoprotein gene of phycobiliprotein from Spirulina and an Escherichia coli expression vector constructed by the recombinant vector. Adopting the modern biotechnology, the present invention clones the Alpha subunit gene of phycobiliprotein from Spirulina from algae mud, constructs the microorganism expression vector and transfers the expression vector into Escherichia coli, which can be easily purified and adapted to large-scale industrialized fermentation production, to construct high secretion type engineering bacteria, thus realizing the high-efficiency, low-cost production of the Alpha subunit holoprotein of phycobiliprotein.