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Application of sesamum indicum SiERF103 gene in enhancing plant resistance

A sesame and gene technology, applied in the application field of sesame SiERF103 gene in enhancing plant resistance, can solve the problems of lack of drought resistance in sesame, few molecular-related research reports, etc., so as to promote stable crop yield, improve plant waterlogging, and improve drought resistance. sexual effect

Active Publication Date: 2021-11-09
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the functions of sesame drought resistance and waterlogging tolerance related genes is still relatively scarce, and there are relatively few molecular related research reports. Therefore, the discovery of sesame stress resistance functional genes is of great significance for the development of sesame stress resistance molecular breeding and the improvement of sesame stress resistance.

Method used

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  • Application of sesamum indicum SiERF103 gene in enhancing plant resistance
  • Application of sesamum indicum SiERF103 gene in enhancing plant resistance
  • Application of sesamum indicum SiERF103 gene in enhancing plant resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1 Sedimentary Gastronium Construction of SiERF103 gene

[0023] 1, sesame Sierf103 gene acquisition

[0024] 114 sesame ERF genes were identified by homologous sequence comparison analysis using the protein sequence of the Arabidopsis ERF gene, including the SIERF103 gene (gene login number: LOC105175593).

[0025] To analyze the function of the Sierf103 gene in adverse resistance, the primer of its complete CDS sequence can be amplified according to the CDS sequence of the SIERF103 gene. Take the primary flowering stage anti-continent sesame seeds, the root tissue after 7 days of seal 75, using EasySpin Plus plant RNA extraction kit (purchased from AIDLAB), extracts total RNA, using reverse transcriptase (purchased from Vazyme) Its reverse transcription synthesis cDNA (method is operated according to Vazyme Reverse Reproductive Enzyme Reagent Manual). The conventional PCR amplification was performed with the design primer after the CDNA after the reverse transcript...

Embodiment 2

[0037] Example 2 Application of Sesame Sierf103 gene in enhancing plant stains and drought resistance

[0038] 1, transfer SIERF103 agonism

[0039] (1) Recombinant carrier transfer to Agrobacterium LBA4404

[0040] 1 2 μg of plasmid DNA was added to each 100 μl of LBA4404 Agrobacterium in Agrobacterium, and the bottom is gently mixed with hand and mix it, and then allowed to stand for 5 min, 5 min of liquid nitrogen, 37 ° C water bath, ice bath 5 min.

[0041] 2 Add 700 μl of an antibiotic LB liquid medium, and cultured at 28 ° C for 5 h. 13,000 rpm Centrifuge 1min collected bacteria, retake more than about 100 μl of supernatant, gently blowing heavy suspensions, uniformly coated it on LB solid medium containing KAN and RIF, and incubated in the incubation of 2 days, Several positive clones were picked, and colonus PCR was carried out by primers SIERF103FL-F / R in Example 1, and the carrier PCAMBIA1301S-SIERF103 has been transferred into root cancer Agrobacterium LBA4404.

[004...

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Abstract

The invention discloses application of a sesamum indicum SiERF103 gene in enhancing plant resistance, and belongs to the technical field of plant genetic engineering, wherein a nucleotide sequence of the sesamum indicum SiERF103 gene is shown as SEQ ID NO.1, and the resistance comprises waterlogging resistance and / or drought resistance. The invention reports the application of the sesamum indicum SiERF103 gene in enhancing plant waterlogging resistance and / or drought resistance for the first time. By constructing an overexpression vector of the SiERF103 gene, the SiERF103 gene is overexpressed in arabidopsis thaliana, drought or waterlogging stress treatment is carried out, and research finds that normal growth conditions are recovered after the drought or waterlogging stress treatment, so that a large amount of transgenic arabidopsis thaliana can recover normal growth. The invention proves that the sesamum indicum SiERF103 gene has a function of improving plant waterlogging and drought resistance, which provides genetic resources for genetic improvement of crop drought resistance and waterlogging tolerance, and is of great significance for promoting crop stability.

Description

Technical field [0001] The present invention belongs to the field of plant gene engineering, and the application of sesame sierf103 gene in enhancing plant resistance. Background technique [0002] Sesamum Indicum L.) is a special oil crop in my country. It has been cultivated in history. It has grown all over the country. In recent years, my country's sesame seeds have fallen seriously, which has a great relationship with the effects of non-biological stress such as drought and water during growth and development. The Sesame Industrial Technology System has been investigated and analyzed by most of my country's sesame homes. my country's sesame seeds have been affected by stress and drought stress, which seriously affects sesame growth, which is an important cause of sesame production. Therefore, increasing sesame tonar and drought resistance, promoting high-yield stabilization, which is especially important for promoting the development of sesame production in my country. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/84A01H5/00A01H6/20
CPCC07K14/415C12N15/8271C12N15/8273
Inventor 游均苏如奇刘爱丽黎冬华张秀荣
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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