211 results about "Anoectochilus formosanus" patented technology

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

The invention discloses a greenhouse cultivation method for anoectochilus formosanus. The greenhouse cultivation method comprises the four steps of acclimating anoectochilus formosanus seedlings, disinfecting a cultivation substrate, planting, and controlling temperature and light, wherein in the step of planting, a cultivation cup after the anoectochilus formosanus seedlings are planted is placed into a plastic bag, and a plurality of small holes are formed in the position where the plastic bag higher than the cultivation cup; and water is poured into the bottom of the plastic bag, a bag opening in the plastic bag is sealed, and the plastic bag is hung below a seedling bed in a greenhouse. Due to the adoption of the technical scheme, the water is evaporated in the bag, so that the water is not liable to run away and a wetting environment suitable for growth of the anoectochilus formosanus is formed in the plastic bag. The anoectochilus formosanus seedlings are spaced from the outside environment through the plastic bag, the water is not liable to run away and the anoectochilus formosanus seedlings do not need to be watered in the later period after being planted, so that the anoectochilus formosanus seedlings cannot be polluted by various bacteria in air or water and the pest and disease damage is avoided. Therefore, higher survival rate of the anoectochilus formosanus seedlings can be also guaranteed in the absence of farm chemicals.

The invention relates to hormone-free tissue culture and a rapid propagation method of anoectochilus formosanus seedlings. The method comprises the following four tissue culture stages: 1) seeds germin ate without bacteria to form protocorms; 2) the protocorms is propagated and differentiation culture is performed on plantlets; 3) strong seedlings of aseptic seedlings are cultured; 4) rooting culture is performed, and different hormone-free culture media are adopted at different tissue culture stages. The method provided by the invention has the advantages that the operation is simple and convenient, the process is less, the cost is low, frequent subculture is not required to be performed on tissue culture seedlings, exogenous plant hormones are not used during the whole culture process, the problems that a large number of exogenous plant hormones are used, the production cost is high, the seedlings are irregular, and the seedlings are unsafe to eat exist in the conventional process of culturing anoectochilus formosanus tissues are solved better, the quality and the transplanting survival rate of the seedlings are improved, and security of using the seedlings is improved.

The invention relates to a multi-flavor loquat flower tea, and belongs to the field of tea technology. the multi-flavor loquat flower tea is prepared by the following steps: cleaning loquat leaf, Anoectochilus formosanus, stevia rebaudiana leaves, licorice, Buddha's Lamp and rose flower, adding water, decocting, fetching juice, steaming loquat flower with juice, and then drying the steamed loquat flower. The multi-flavor loquat flower tea provided by the invention has the advantages of fragrance, fresh mouthfeel, diversed mouthfeel and nutrition and health compositions. The invention has extremely high amino acid and trace element contents, and has the nutrition, anti-aging, and human body immunity adjusting health care effect. The product does not contain any additives and is a green health care drink. The effective ingredients and unique local flavor of the loquat flower provided by the invention are reserved successfully, and other flower fragrance and traditional Chinese medicine components are added so that the multi-flavor loquat flower tea has high nutritive value.

The present invention relates to an application technique of mycorrhizal fungi in cultivation of anoectochilus formosanus. It is characterized by that the used mycena-generic four fungi respectively are mycena dendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. First, the mycorrhizal fungi and seedling of anoectochilus formosanus are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of anoectochilus formosanus, and can raise its yield and effective component content.

The invention discloses a method for producing anoectochilus formosanus by tissue culture, comprising the following steps: frond selection and asepsis, cluster bud inducing culture and large-scale culture. The key to the technique is characterized in that two different culture media are applied to the inducing culture and the large-scale culture, wherein the inducing culture medium is as follows: H culture medium+ 10-50g/L of cane sugar + 0.1-3.0mg/L of alpha-naphthylacetic acid+ 0.1-5.0mg/L of 6-benzyladenine, and pH value is 5.0-7.0; the large-scale culture medium is as follows: B5 culture medium+ 20-40g/L of cane sugar+ 6-8g/L of agar+ 2.0-3.0g/L of active carbon+ 1.0-2.0mg/L of alpha-naphthylacetic acid+ 2.0-3.0mg/L of benzyladenine, and pH value is 5.5-6.5. The method is easy to operate, features low production cost, high output, high quality and no pollution to the environment and can realize batch production.

The invention provides an interplanting cultivation device for anoectochilus formosanus and dendrobium officinale. The interplanting cultivation device comprises a thin film greenhouse and an interplanting shelf placed in the thin film greenhouse. The interplanting shelf is a three-dimensional triangular shelf formed by two longitudinal isosceles triangle frames and three transverse parallel rectangular frames. Dendrobium officinale planting frames formed by mutually stacking trunks and/or bamboo rods are placed on an upper layer and a middle layer. An anoectochilus formosanus planting tray shelf is arranged on a lower layer. The dendrobium officinale is planted into gaps among the upper and lower trunks of the upper layer and the middle layer of the interplanting shelf. The anoectochilus formosanus is planted in planting trays, with matrixes, on the lower layer of the shelf. The relative humidity in the thin film greenhouse is controlled to be 70-90%, the temperature is controlled to be 18-25 DEG C, the light transmittance is 60-80%, and spraying and ventilating facilities are arranged. Roots of the dendrobium officinale adhere to the trunks so as to shade the anoectochilus formosanus on the lower layer, the anoectochilus formosanus on the lower layer is cultivated in a lifting mode by adopting the matrixes, the water permeability is good, soil insect harm can be avoided, organic fertilizer sprayed from the upper layer can be fully utilized, the planting cost is lower, the harvesting reasons are spaced, and benefits are higher.

The invention discloses a method for planting anoectochilus formosanus in the northern area. The method comprises the following steps: (1) woodland selection, (2) seedling selection, (3) seedbed preparation, wherein the northern area with the temperature in spring, summer and autumn ranging from 15 DEG C to 30 DEG C is selected as the cultivation field, soil under chestnut trees is selected, soil texture is required to be loose, breathable and good in moisture retention, the matrix on the ridge surface is formed by adopting and evenly stirring and mixing 1/2-2/3 of organic fertilizer and 1/3-1/2 of under-wood fine soil humus, before plantation, spraying and sterilizing are carried out through carbendazim or thiophanate, plastic films cover the matrix for 2-3 days and then are uncovered, and the matrix is prepared for use, (4) transplantation, (5) management after plantation, and (6) harvest. According to the method, a complete set of the technology for planting the anoectochilus formosanus under woods in the northern area is formed. The wild environment is imitated, local natural products are explored and utilized for mixing preparation to form the matrix for transplantation, and therefore the production cost is low; the cultivation technology for improving the plantation survival rate of the anoectochilus formosanus is formed, and therefore the stable yield is achieved easily; by the adoption of the method, the anoectochilus formosanus grows normally, and the net weight of a single plant is increased.

The invention discloses a rapid proliferation method for anoectochilus formosanus tissue culture, which comprises the steps: cleaning and disinfecting an explant, inoculating the explant after cleaning and disinfecting in an MS culture medium, performing low light culture for one week, then performing strong light culture for one week, wherein the illumination time is 10 hours per day, and the temperature is 23+/-2 DEG C; culturing for 40 days; inoculating the explants after culturing in a liquid culture medium of the MS culture medium, 1.4-1.6mg/L of 6-BA and 0.2-0.3mg/L of NAA in a ratio for enrichment culture under a dark culture condition, wherein the temperature is 23+/-2 DEG C, and the culture period is 45 days; and by using a strong seedling culture medium as the MS culture medium, performing illumination culture, wherein the illumination time is 8 hours per day, the temperature is 23+/-2 DEG C, the culture period is 12-15 days, and the ratio of a rooting culture medium is as follows: the content of MS is 1/2, the content of bananas is 60g/L, the content of active carbon is 1g/L, the content of casein hydrolysate is 0.5g/L, the illumination time is 10 hours per day, the temperature is 23+/-2 DEG C, and the culture period is 3 months. According to the rapid proliferation method for the anoectochilus formosanus tissue culture, the industrialized rapid proliferation is realized, and the culture period is shortened.

The invention belongs to field of artificial crop seed production technology, and provides a key technology for producing artificial seed of gold thread lotus which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of gold thresd lotus and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

The invention relates to a greenhouse soil planting method of anoectochilus formosanus, which belongs to the field of an agricultural planting technology. The greenhouse soil planting method of the anoectochilus formosanus comprises the following preparation steps of: establishing a bamboo greenhouse and installing sprinkler irrigation facilities; then choosing peat soil as a planting matrix, choosing anoectochilus formosanus seedlings to plant; paying attention to regulating temperature, humidity, luminosity and ventilation in the bamboo greenhouse in a secondary planting period of the anoectochilus formosanus so as to benefit the growth of the anoectochilus formosanus. The greenhouse soil planting method of the anoectochilus formosanus, provided by the invention, is good in safety, high in quality and harmless to human body, and is not only suitable for small-size production based on the family unit, but also suitable for large-scale industrial production.

The invention relates to an auxiliary material and cultivation method for realizing field growth of anoectochilus formosanus. According to the auxiliary material, the weight mixing ratio of soddy soil is more than 99.5 percent, and the ratio of lime is less than 0.5 percent. A spinney area close to water flow and waterfall with the canopy density of more than 78 percent is selected as a cultivation area. The method comprises the following steps: mixing the auxiliary material with the thickness of 7cm and deeply ploughed surface soil, thoroughly watering and retting for 30 days, washing the seedlings without sterilization, planting according to a spacing between rows of 3*5cm, spraying bactericides in a mist shape and watering rooting water, spraying the bactericides every 10 days in the earlier 30 days, spraying a solution of biofertilizers and monopotassium phosphate outside the roots every 15 days after 30 days for 3-5 times, utilizing clean spring water for cultivation, covering a shading net on the spinney area in fine days and covering a film in rainy days on the spinney area, wherein two sides of the net and the film need to be 20cm away from the ground, and two ports are not shaded. The survival rate is 90 percent. The investment on infrastructures is avoided, natural resource bed soil cultivation is utilized, soil fertility, water, bacteria, ventilation and illumination are regulated, return-to-nature growth of anoectochilus formosanus is realized, the auxiliary material is low in investment, slight in pollution and high in yield and suitable for large-scale cultivation, and the fresh product is similar to a wild plant.

The invention discloses an underwood planting method of Emei Anoectochilus formosanus. The whole process does not need too many infrastructures, the method is economical and suitable, low in cost, simple in operation, easy to master and high in yield, and the incidence rates of diseases and insect pests are low. The method comprises the following steps of site selection of a woodland, preparation of a seedbed, cleaning and transplanting of tissue culture seedlings, irrigation and fertilization and field management, pest and disease control and harvesting. The Emei Anoectochilus formosanus planted for 4-6 months is adopted is basically consistent with the wild type Emei Anoectochilus formosanus in quality, color, luster and other aspects.

The invention relates to a compound oral solution for relieving alcoholism and protecting the liver and a preparation method thereof, in particular to an anoectochilus formosanus compound oral solution for relieving alcoholism and protecting the liver and a preparation method thereof. The oral solution comprises the following components in proportion by weight: 50-65% of anoectochilus formosanus,5-10% of wild chrysanthemum flower, 4-10% of white peony root, 4-10% of dried rehmannia root, 5-10% of flower of kudzuvine, 5-10% of sealwort, 3-6% of glehnia root, 2-5% of southernwood, 2-6% of ebony and 1-3% of liquorice. The preparation method comprises the steps of extracting, mixing, concentrating, clarifying, correcting, roughly filtering, finely filtering, sealing, sterilizing and packing finished products. The compound oral solution has a pure traditional Chinese medicine combination, a scientific prescription and an advanced process, and the product has obvious functions of relievingalcoholism and protecting the liver, can obviously relieve headache, dizziness, sleepiness, asthenia, thirst, vomit, fantod and other symptoms caused by insobriety or drunkenness, has the health carepreventive functions of soothing the liver and strengthening the spleen, restoring consciousness and soothing the nerves, clearing away heat and toxic material, and invigorating qi and nourishing theblood, and also has the characteristics of good taste and flavor and taking convenience.

The invention discloses a molecular marker fingerprinting for identifying the anoectochilus formosanus produced at Huoshan in Anhui and the similar species thereof. The molecular marker fingerprinting comprises the steps of preprocessing anoectochilus formosanus samples, extracting DNA of each sample genome, executing ISSR-PCR (inter-simple sequence repeat-polymerase chain reaction), executing agarose gel electrophoresis, building ISSR molecular marker fingerprint of each sample and using the ISSR molecular marker fingerprint for identifying the anoectochilus formosanus germplasm. The method provided by the invention saves time and economic cost, and can obtain the accurate and reliable identification fingerprint just by extracting the DNA of the anoectochilus formosanus plants, executing ISSR-PCR and executing agarose gel electrophoresis; the method identifies the species, which are easy to confuse in appearance, from the essence of inheritance, has the advantages of being simple, convenient and efficient, excellent in repeatability and high in resolution, and so on; and the method can identify the species in seeding stage of plants, and plays an important role in guaranteeing the accuracy and the stability of the Huoshan anoectochilus formosanus crude drug base source.

The invention provides cultivating soil for anoectochilus formosanus. The cultivating soil is composed of, by mass, 20-25 parts of vermiculite, 25-30 parts of peat soil, 15-18 parts of pine needle soil, 15-18 parts of oak leave soil, 10-15 parts of sand, 1.5-2.5 parts of EM stock solution and water used for wetting the components. According to the cultivating soil for anoectochilus formosanus, a new formula is used, the field growing environment of anoectochilus formosanus is simulated, nutrition is sufficient, nutrient fertilizer does not need to be dressed in one growth cycle, under the situation of daily temperature, humidity and illumination, the survival rate is high, and the growth vigor is good.

The invention relates to traditional Chinese medicine liquid for treating diabetes, prepared by the following steps of: selecting bidens pilosa, betony, evolvulus alsinoides, Japanese polygala, balloon vine, barbat skullcap, herba portulacae, sambucus chinensis, buddhas lamp twig and leaf, pogonatherum crinitum, herba osbeckiae, anoectochilus formosanus hayata, Chinese lespedeza and clinopodium gracile, removing impurities, then washing and chipping; putting together in proportion, adding a proper amount of water for soaking for 24-30 hours, boiling to 100 DEG C, continuing decocting for 2-30 minutes, and then filtering to obtain the liquid medicine, namely the traditional Chinese medicine liquid. The traditional Chinese medicine liquid is an externally applied agent for treating diabetes, is painless and has no toxic side effect when used by patients, and has abundant sources of raw materials, low price and convenience in preparation.

The invention discloses a method for cultivating anoectochilus formosanus by using a culture bag. A transparent sealing plastic bag is used for replacing a traditional glass culture bottle to serve as a culture container; and according to different growth phases of the anoectochilus formosanus, an induction culture medium, a proliferation culture medium, a strong seedling culture medium or a rooting culture medium is injected into the culture bag, so that anoectochilus formosanus explants are subjected to tissue culture and seedling culture. When the anoectochilus formosanus is subjected to the tissue culture by adopting the method, the pollution can be effectively controlled, and the contamination rate is smaller than 3%; an inoculation progress is simplified and damages to the seedlings are reduced; an inoculating speed is improved and anoectochilus formosanus tissue culture seedlings with strong disease resistance are obtained; and meanwhile, the operation of taking out seedlings from the culture bottle after bagged tissue culture also can be avoided, and integration of tissue culture, seedling growing and product transportation of the anoectochilus formosanus is realized, so that the production cost is greatly reduced, the yield is improved and the growth period is shortened.

The invention discloses purple sweet potato vermicelli with a function of enriching blood and a processing method of the purple sweet potato vermicelli. The purple sweet potato vermicelli is prepared from the following raw materials in parts by weight: 80-100 of purple sweet potatoes, 15-25 of black glutinous rice, 15-25 of chickpea, 15-20 of ormosia, 10-15 of blueberry, 10-15 of daylily, 2-4 of apple flower, 1-2 of lance asiabell root, 1-2 of anoectochilus formosanus, 1-2 of sargentg loryvine stem, 2-3 of roselle flower, 1-2 of sea cucumber powder, 5-8 of grape seed powder, 20-30 of polygonum multiflorum starch, 8-10 of nutritional additive and a proper amount of mulberry wine. The purple sweet potato vermicelli with the function of enriching the blood is rich and balanced in nutrition and is fragrant, sticky, soft, smooth, tough, tender, tasty and refreshing; as multiple kinds of beneficial Chinese herbal medicinal ingredients are added to be complementary with nutritional foods, the purple sweet potato vermicelli has good functions of enriching the blood and invigorating blood circulation, is particular suitable for female with anemia to eat, is capable of nourishing and strengthening body by being frequently eaten and is capable of enabling skin to be ruddy and bright while whitening the skin.

The invention discloses an underwood planting method of anoectochilus formosanus. The method is characterized by including the steps that first, forest land is prepared, wherein, a plurality of water spraying pipes are arranged above a forest site, an underwood soil layer is ploughed so as to loosen and break the soil layer, and a dead leaf layer with the thickness ranging from 2 cm to 3 cm is laid above the ploughed soil layer; second, anoectochilus formosanus seedlings are planted, wherein the anoectochilus formosanus seedlings are obtained through tissue culture and planted into the ploughed soil layer; third, underwood management is performed, wherein in the growth stage of the anoectochilus formosanus seedlings, when the dead leaf layer is in a dried state, the water spraying pipes are adopted for spraying water, and therefore the dead leaf layer can be kept moist. According to the method, dead leaves are stimulated to decompose with a fermentation method to generate small molecule nutrient substances capable of being easily absorbed by anoectochilus formosanus, meanwhile, dead leave powder can provide a good moist environment for anoectochilus formosanus, and therefore the fresh weight of the anoectochilus formosanus and the content of medical composition of the anoectochilus formosanus are increased.

The invention relates to a health-care product for reducing blood sugar and a method for preparing the same, in particular to a health-care product of anoectochilus formosanus and pumpkin composite blood sugar reduction series and a method for preparing the same. The method adopts the following technical proposal that: the health-care product contains anoectochilus formosanus and pumpkin and is prepared by mixing the anoectochilus formosanus powder and pumpkin powder; and the weight percentage of the anoectochilus formosanus powder to the pumpkin powder is 1:1-5. The anoectochilus formosanus and pumpkin are compounded to prepare various dosage forms which comprise capsules, oral liquid, beverage and the like. The series of the health-care product has medical health-care effects of blood sugar reduction of the anoectochilus formosanus and pumpkin, has no toxicity and no side effect, is suitable for various groups and also has good health-care effect on healthy people.

The invention provides an anoectochilus formosanus compound alcohol-relieving and liver-protecting capsule and a preparation method thereof, and belongs to the technical field of medicine or health-care foods. The capsule comprises the following components in percentage by weight: 50 to 65 percent of anoectochilus formosanus, 5 to 10 percent of wild chrysanthemum, 4 to 10 percent of white peony root, 4 to 10 percent of raw rehmannia root, 5 to 10 percent of flower of kudzuvine, 5 to 10 percent of cordate houttuynia, 3 to 6 percent of tree peony bark, 2 to 5 percent of sweet wormwood, 2 to 6 percent of olive, and 1 to 3 percent of liquorice. The preparation method comprises the following steps of: extracting; concentrating; spray-drying; preparing; screening; and packaging into a finished product. The capsule has pure Chinese medicinal composition, scientific prescription and advanced process, has remarkable function of relieving alcohol and protecting liver to obviously relive headache, dizziness, drowsiness, weakness, thirst, sickness, fantod and other symptoms caused by insobriety or drunkenness, has health-care and prevention functions of soothing liver and strengthening spleen, restoring consciousness and soothing nerves, clearing heat and removing toxicity, and invigorating vital energy to nourish the blood, and has the characteristics of convenience in carrying and storage.