Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit

Abstract

The invention discloses a hormone-free rapid propagation organic production method of anoectochilus formosanus germchits. The method comprises the following steps of: step 1, sterilely germinating seeds, including disinfecting anoectochilus formosanus capsules and taking out the seeds, and inoculating the taken seeds on a culture medium I to be cultured until the seeds are germinated to protocorms; step 2, carrying out proliferation differentiation on the protocorms, including transferring the protocormss formed from seed germination to a culture medium II to have proliferation differentiation cultivation; and step 3, growing roots from strong seedlings, including transferring the protocorms to a culture medium III to have strong seedling root growth cultivation when the differentiation buddings of the protocorms grow to 1.5-2.5cm, and carrying out seeding hardening and transplantation when the seedlings grow to 5-6cm high and the roots are 2-3cm. According to the method, a technical route of seed, protocorm and germchit is adopted, and no any hormone is added into the culture mediums used during the cultivation process, so that on the basis that the rapid germination of the anoectochilus formosanus seeds is ensured, the purpose that the produced germchits have no residual exceeding is ensured, and germchits of high quality are provided for producing organic products of anoectochilus formosanus.

Description

Technical field [0001] The invention relates to the field of agricultural sciences, in particular to a fast-propagating organic production method for hormone-free seedlings of Anoectochilus. Background technique [0002] Orchidaceae in my country (Orchidaceae) Anoectochilus Bl.) There are about 23 species, some of which are whole grasses for folk medicine. They are rare and precious Chinese medicinal materials and are commonly known as golden thread lotus. Anoectochilus is a perennial herbaceous plant. The research and development are: Anoectochilus ( Anoectochilus roxburghii (Wall.) Lindl.), also known as the flower-leaf orchid; Taiwan silver-line orchid ( Anoectochilus formosanus Hayata), also known as Anoectochilus (Taiwan Flora), Taiwan Anoectochilus (Taiwan Flora, Taiwan Orchid Plant Color Illustration); Anoectochilus xingrenensis Z.H.Tsi et X.H.Jin) and so on. Folks are mostly used to treat diabetes, hyperlipidemia, hepatitis B and other diseases, and are known as "kin...

AI Technical Summary

Problems solved by technology

Clematis clematis is an expensive health-care Chinese medicinal material, and product quality and safety are more important. Existing tissue culture techniques add phytohormones, which will cause hormones to be enriched in clematis seedlings. Due to artificial planting of clematis The time from planting to harvesting of lotus is short, hormone residues are difficult to solve, and the product quality cannot meet the standards of organic products

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