Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds

Abstract

The invention discloses a hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds. According to the hormone-free rapid tissue culture and propagation method, no hormone is used in the whole process, the mature and non-cracking fruits, which is pollinated for 5 months after flowering, of wild or artificially planted roxburgh anoectochilus terminal bud distributed in Szechuan Emei region are taken as the material, and seedlings are bred efficiently and quickly according to the route from seeds to protocorms, complete plants and acclimatization and transplant. A hormone-free culture medium is adopted in the whole process; more than 5000 good-quality seedlings can be quickly bred from one mature fruit by virtue of tissue culture of about 10-12 months, and after acclimatization and transplant, the greenhouse culture survival rate can be above 95%. According to The hormone-free rapid tissue culture and propagation method of the Emei roxburgh anoectochilus terminal bud seeds is capable of keeping the good traits of the Emei roxburgh anoectochilus terminal bud and maintaining the good medicinal properties of the Emei roxburgh anoectochilus terminal bud; besides, the method is simple, and the cost is low; industrialized seedling production can be realized; no hormone is adopted so that the safety of the seedlings can be ensured; the hormone-free rapid tissue culture and propagation method has important significance on regeneration and sustainable utilization of critically endangered valuable and rare resources of medicinal plants and protection of seed resources.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for quickly breeding organic seedlings without hormones by using mature capsules of A. emeiensis. Background technique [0002] Emei golden clematis ( Anoectochilus emeiensis K. Y. Lang.) is a member of Orchidaceae (Orchidaceae), Anoectochilus ) perennial herbaceous plants, the same flowers and leaves as the lip orchid, Taiwan, Fujian, Guangdong, Guangxi clematis, etc. are collectively referred to as clematis. It is often used as medicine in the folk, and its amino acid composition, ingredients, content, vitamins, polysaccharides, etc. are higher than those of American ginseng and wild ginseng. It is flat in nature and sweet in taste, has the functions of clearing heat, cooling blood, dispelling wind and dampness, strengthening the heart and diuresis, strengthening the kidney and calming the liver, and lowering blood pressure. It has good eff...

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Problems solved by technology

[0003] At present, the existing researches are mainly concentrated in Taiwan, Fujian, Guangdong and Guangxi A. clematis, and most of them use the stem segment as the explant material for propagation. There is no report on the rapid propagation of seedlings of A. emei clematis capsules.

Moreover, the medium used contains more hormones, which will cause the hormones to be enriched in the clematis seedlings, and the product quality cannot reach the standard of organic products. For this reason, the research on artificial rapid cultivation of clematis seeds without hormones is carried out, and a set of methods is established. The tissue culture and rapid propagation system of seedlings is of great significance to the rational protection and development of A. emei germplasm resources and the industrialization and large-scale production of organic seedlings

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