69results about How to "Realize factory breeding" patented technology

The invention belongs to the technical field of plant tissue culture and particularly relates to a factory seedling cultivating method of actinidia arguta. The method is mainly technically characterized by comprising the five steps of: preparing culture media, selecting and disinfecting explants, inoculating, subculturing and transplanting in a sand bed. The factory seedling cultivating method of actinidia arguta is mainly provided on account of the conditions that actinidia arguta is in a wild state in all northeast areas, and the traditional cultivating modes which comprise sowing propagation, cuttage propagation and grafting propagation cause the problems that the variation of offspring characters is serious, male plants are difficult to separate from female plants, resources are seriously damaged, the seedling cultivating speed is low, the characters of the female plants are difficult to keep, the plants are easily infected with bacteria, the survival rate of the seedlings is low, and the like. Induced buds are subcultured and then directly transplanted in the sand bed, the rooting culture process is omitted, the seedling cultivating period is shortened by 50-60 days, the seedling cultivating cost is greatly lowered, the survival rate of the seedlings is obviously increased, the plant infection rate is reduced, excellent female plant characters are kept, and the variety degrading problem is solved.

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

The invention discloses a method for producing a Fujian jewel orchid by plant tissue culture, which comprises the following three procedures: selection and sterile treatment of plants, induced culture of cluster buds and large-scale culture. The method is technically characterized in that two different culture media are used in the induced culture procedure and the large-scale culture procedure, wherein the culture medium for the induced culture comprises an MS culture medium, sucrose of 10-50g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 0.1-3.0mg/L and 6-benzylaminopurine of 0.1-5.0mg/L, and the pH value is 5.0-7.0; and the culture medium for the large-scale culture comprises a modified MS culture medium, calcium nitrate of 600mg/L, mashed banana of 30-50g/L, sucrose of 20-40g/L, agar of 6-8g/L, activated carbon of 2.0-3.0g/L, naphthylacetic acid of 1.0-2.0mg/L and 6-benzylaminopurine of 2.0-3.0mg/L, and the pH value is 5.0-7.0. Plant tissue culture can keep the good characters of the plants. The method has the advantages of easy operation, low production cost, high yield, good quality and no environment pollution, is suitable for industrial seedling raising and can realize mass production.

A method for reproducing pyrmotide includes such steps as buying the developed pyrmotides, storing in cold storage case at -5-15 deg.C, choosing the bee larva, silkworm pupa, or grub as host, putting it in the internal plastic culture utensil, preferably selecting seed pyrmotide, inoculating it to said host, and test tube culture to obtain the second generation pyrmotide. It culture device is composed of a culture tray made of stainless steel and several pairs of internal and external culture utensils. A method for releasing the pyrmotides features that the cultured pyrmotides are loaded from test tubes to an open release box.

The invention provides a method for tissue culture and rapid propagation of camellia oleifera. The method for tissue culture and rapid propagation of the camellia oleifera comprises the steps of explant sterilization, primary culture, subculture, rooting culture and transplant. A preparation method of a rooting medium comprises the steps that a 1/2 MS agar culture medium containing 0.5mg / L IBA is prepared, wherein agar is 7-10 g / L, and the PH value is 5.0-5.5; pulverized perlite is well wrapped by means of a net bag and then is placed into a molten culture medium, the culture medium is fully absorbed by the perlite, then the perlite is drained off slightly and sterilized, and then the rooting medium is obtained. According to the method for tissue culture and rapid propagation of the camellia oleifera, the perlite with a certain granularity is used as the culture medium, the culture medium and the agar culture medium are mixed to form the rooting medium, therefore, the root system of the camellia oleifera can breathe fully, the rooting percentage of camellia oleifera cultivation is greatly improved, the root system is developed, the survival rate of transplanting is high, and a technical base is provided for industrialized seedling production and genetic engineering improvement of tissue culture of the camellia oleifera.

The invention belongs to the technical field of plant propagation and particularly relates to a rapid propagation method suitable for multiple kiwi fruit varieties. The method includes the steps of preparation of a propagation material, soaking, germination accelerating, root promoting, transplanting, seedling hardening and the like. The method is suitable for factory-like rapid propagation of actinidia arguta, actinidia giraldii and actinidia melanandra, the technical problems that in a conventional cutting technology, the seedling culture period is long, and the seedling forming rate is low are solved, and a vegetative propagation method with general applicability is explored. By means of the method, only about 15 days is needed usually, the germinating and rooting rate of branch sections of the actinidia arguta, the actinidia giraldii and the actinidia melanandra can reach 95% or above, and seedlings grow robustly. The method is simple in procedure and easy to operate, and has multiple purposes, the seedling culture cost is reduced, the method is in accordance with the practical operation situation, and the overall benefits of the seedling culture factories are increased.

The invention discloses an industrialized production method for nesidiocoris tenuis, and belongs to the technical field of biological control over agricultural pests. The method comprises the steps of legume crop cultivation, sterilization of glasshouse soil of rice moth eggs and nesidiocoris tenuis raising and collecting. According to the industrialized production method for the nesidiocoris tenuis, a series of technical problems such as plant source and animal source feed combination, plant oviposition materials and raising media are solved successfully, the problems that at present, the nesidiocoris tenuis can only be raised on a small scale in a laboratory, and the control requirements in actual production cannot be met are changed, and the industrialized production efficiency of the nesidiocoris tenuis is greatly improved. By taking the nesidiocoris tenuis as a predatory enemy to be applied to vegetables in a large area, the number of use and the use amount of insecticide can be greatly lowered, an agricultural ecological environment is protected, and the food safety is improved.

The invention discloses a soilless culture seedling raising method for preserved vegetables. According to the method, preserved vegetable seeds are sowed to a seedling raising tray filled with seedling raising matrixes at first, the seedling raising matrixes are formed by mixing 20-50% of rock wool, 30-60% of turf and 5-20% of rotten preserved vegetable leaf residues according to the volume ratio, then the seedling raising tray is transferred to a water pool in a vegetable cultivation greenhouse, the water pool contains water and seedling raising nutrient solutions, the seedling raising tray floats on the water surface of the water pool, greenhouse soilless seedling raising is carried out, and preserved vegetable seedlings are obtained after 12-18 days. Compared with a traditional soil seeding raising method for preserved vegetables, the soilless culture seedling raising method has the advantages of reducing the pest and disease damage rate and cost, improving the survival rate and yield and being high in disease resistance and capable of raising the seedlings in the industrial scale.

The invention discloses a minimal medium, a pinellia ternate tissue culture medium and a pinellia ternate tissue culture method. The minimal medium comprises macroelement components A, macroelement components B, microelement components, organic element components and iron salt components. The pinellia ternate minimal medium based on the minimal medium by proportioning is established, and a pinellia ternate cluster bud induction culture medium, a pinellia ternate cluster bud proliferation culture medium and a pinellia ternate rooting culture medium which are prepared on the basis of the pinellia ternate minimal medium and are involved in the culture method are selected and optimized; the minimal medium has the advantages of high bud induction rate, high proliferation coefficient, high rooting rate, fast rooting, developed root system, thick plants, high transplanting survival rate and low cost, industrialized seedling cultivation of pinellia ternate tissue culture is achieved, and application and popularization are facilitated.

The invention discloses a high-altitude-area Hawk tea asexual cuttage method. The method comprises the steps of cutting cuttage branches, using a hormone solution to soak the cuttage branches, pavinga cuttage seedbed with a water-retention layer, conducting cuttage, conducting greenhouse cultivation, lifting seedlings and the like. According to the high-altitude-area Hawk tea asexual cuttage method, asexual cuttage is adopted to propagate Hawk tea seedlings, the operation is simple, the labor force is saved, the operation is not influenced by the external temperature environment, the cuttagesurvival rate is high, industrialized seedling growing is achieved, and the production capability of Hawk tea seedlings is greatly improved.

The invention relates to the technical field of tissue culture, in particular to a culture medium for coriaceous euonymus tissue culture and a method thereof. The culture medium for coriaceous euonymus tissue culture comprises an initial culture medium, a proliferation culture medium and a rooting culture medium. The culture medium can remarkably improve the survival rate and transplanting survival rate of the initially cultured coriaceous euonymus, the initiated culture survival rate is improved from existing below 30% to 85%, and the transplanting survival rate of seedlings is improved from existing 50% to existing above 90%. By utilization of the plant tissue culture method, the problems of long period and low reproduction rate of a traditional coriaceous euonymus reproduction mode are solved, a rapid coriaceous euonymus reproduction and culture system is formed, and industrialized seedling raising can be achieved.

The invention discloses a fast reproduction method for energy willow tissue culture, which comprises the following steps: (A) selection of an explant; (B) disinfection of the explant: removing the leaves of the steam of the explant, placing the washed branch into a clean bench, cleaning in an ethanol solution, washing with sterile water, disinfecting with mercury chloride and flushing with sterile water; (C) primary culture; (D) enrichment culture: introducing the primary-culture stem in good growth into an enrichment culture medium; (E) rooting culture; and (F) seedling hardening and transplanting: opening a bottle cover of a rooting culture medium, culturing at room temperature, taking out the seedling, and transplanting to a vermiculite turfy soil matrix. In the method, by adopting reasonable and scientific culture method and steps and controlling reasonable parameters, the quality of the cultured energy willow tissue is very good, the sound field of the energy willow is fast, and an important guarantee is provided for realizing large-scale industrialized seedling production of energy willow and accelerating the popularization and application of good germ plasm.

The invention discloses a container sleeved root control bag seed and seedling cultivation device, an automatic-control irrigation system thereof and a seed and seedling cultivation method. Strong seeds or seedlings to be cultivated are treated and then planted in container sleeved root control bags, irrigation is performed by the automatic-control irrigation system, and fertilizing is realized through irrigation during growing. Pipelines are used to connect each part, so that comprehensive automatic irrigation and water-fertilizer integration in a planting area are realized; by the method, survival rate of seedlings is increased, and the seedlings are uniform in growing speed, so that standardized production of the seedlings is facilitated, factory-like seedling growing can be realized, and various high-quality engineering seedlings complete in specification are cultivated.

The invention discloses an asexual cutting propagation method scale development of hawk tea in low-temperature and alpine regions. The method comprises the steps of cuttings picking, immersion of cuttings in hormone solution, laying of water-preserving layers on cutting seedbeds, cottage, greenhouse cultivation, seedling lifting and the like. The asexual cutting propagation method scale development of the hawk tea in low-temperature and alpine regions is simple in operation, labor-saving, free from influence of external temperature environment, high in cutting survival rate and capable of achieving industrialized seedling production, thereby greatly improving the productivity of hawk tea seedlings.

The invention relates to a detoxification and propagation expanding method of sweet potatoes. The detoxification and propagation expanding method mainly comprises the steps of explant collection and disinfection treatment, differentiation and induction culture, multiplication culture, rooting culture, seedling hardening and transplanting, field management and timely harvesting. By the adoption ofthe propagation expanding method, under the aseptic condition, through tissue culture and seedling hardening treatment, the transplanting survival rate of detoxification seedlings obtained after tissue culture can reach 100%, the serious problem of sweet potato virus diseases caused by propagation of sweet potato blocks or sweet potato vines in recent years is solved, the problems of variety quality deterioration, germplasm degeneration, yield reduction and the like are effectively prevented, and the germplasm purity is guaranteed; after detoxification, the germplasm is purified on the basis of keeping the variety characteristics of the original variety, the epidemic resistance of the variety is enhanced, and the quality and the yield are improved; and according to the method, the detoxification seedlings which are not limited by seasons and can be multiply and rapidly propagated in a high-coefficient mode can be obtained, industrialized seedling production and high-efficiency production can be achieved, and the detoxification seedlings can be widely applied to production.

The invention discloses a culturing method of Acacia mangium*A. auriculiformis Cunn.ex Bench by ex-vitro rooting. The method disclosed by the invention comprises the following steps: scissoring an Acacia mangium*A. auriculiformis Cunn.ex Bench axillary bud containing stem, and grafting the stem into a primary culture medium; culturing the stem until the height of the axillary bud is 2-3 cm, scissoring the axillary bud, grafting the axillary bud into an enrichment culture medium, and culturing the axillary bud until an enrichment seedling with a height of 4-5 cm is obtained; hardening the enrichment seedling for 5-7 days under natural lighting, and scissoring the seedling into single buds; and after the roots of the buds are soaked for 50-70 min by using 100-150 mg/LIBA, putting the buds into a nutritious cup in a greenhouse to culture by cottage. According to the ex-vitro rooting method disclosed by the invention, a rooting process of an enrichment seedling and a domestication process are combined together, so that the quality and survival rate of tissue culture seedlings are improved, the process of seeding breeding is accelerated, the whole tissue culture link is simplified, the production time is shortened, the culturing space of tissue culture is saved, and the production cost is reduced, thereby facilitating the factory-like seedling raising of Acacia mangium*A. auriculiformis Cunn.ex Bench.

The invention discloses a tissue culture and rapid propagation method and culture medium for an apple continuous cropping-resistant dwarf rootstock G41; the culture medium contains a startup culture medium, a proliferation culture medium and a rooting culture medium, wherein the startup culture medium comprises the components: MS, 6-BA, IBA, NAA, sucrose and agar; the proliferation culture mediumcomprises the components: MS, 6-BA, IBA, sucrose and agar; the rooting culture medium comprises the components: 1/2MS, IAA, IBA, sucrose and agar. Through the startup culture medium and the proliferation culture medium, a stem apex body is subjected to tissue culture treatment; through the rooting culture medium, tissue culture seedlings are subjected to rooting treatment, propagation of cutting and layering are no longer carried out, and the survival rate of transplanted seedlings is increased.