Method for tissue culture and rapid propagation of camellia oleifera

Abstract

The invention provides a method for tissue culture and rapid propagation of camellia oleifera. The method for tissue culture and rapid propagation of the camellia oleifera comprises the steps of explant sterilization, primary culture, subculture, rooting culture and transplant. A preparation method of a rooting medium comprises the steps that a 1 / 2 MS agar culture medium containing 0.5mg / L IBA is prepared, wherein agar is 7-10 g / L, and the PH value is 5.0-5.5; pulverized perlite is well wrapped by means of a net bag and then is placed into a molten culture medium, the culture medium is fully absorbed by the perlite, then the perlite is drained off slightly and sterilized, and then the rooting medium is obtained. According to the method for tissue culture and rapid propagation of the camellia oleifera, the perlite with a certain granularity is used as the culture medium, the culture medium and the agar culture medium are mixed to form the rooting medium, therefore, the root system of the camellia oleifera can breathe fully, the rooting percentage of camellia oleifera cultivation is greatly improved, the root system is developed, the survival rate of transplanting is high, and a technical base is provided for industrialized seedling production and genetic engineering improvement of tissue culture of the camellia oleifera.

Description

technical field [0001] The invention relates to tissue culture technology, in particular to a rapid propagation method of camellia oleifera tissue culture. Background technique [0002] Camellia oleifera (Camellia oleifera) is a plant of the genus Camellia in the family Theaceae. It is a unique high-quality woody oil tree species in my country. It is also known as the world's four largest woody oil tree species together with olive, oil palm and coconut. Camellia oil is rich in nutrition, clear in color and fragrant, and durable in storage. It is one of the high-quality edible oils and is known as "Oriental olive oil". With the improvement of human living standards, the nutritional value of camellia oleifera has been widely recognized, and the market demand for camellia oil has increased sharply. However, the current output of camellia oil is far from meeting the needs of people's daily life. The low yield and low efficiency of camellia oleifera seriously restrict the develop...

AI Technical Summary

Problems solved by technology

At present, Camellia oleifera seedlings are bred mainly by grafting seedlings with anvils, but it takes a long time for seedlings to grow out of the nursery through grafting with anvils, and it is difficult to meet the requirements of the development of the national Camellia oleifera industry for excellent clones of Camellia oleifera. Tissue culture of Camellia oleifera rapidly breeds become an inevitable trend

[0003] Camellia oleifera is a drought-loving plant, and its root system is difficult to take root in airtight conditions. Therefore, the tissue culture of Camellia oleifera generally faces the technical bottleneck of difficult rooting. Although the method of rooting outside the bottle can increase the rooting rate, due to serious pollution such as bacteria, it cannot It meets the requirements of genetic improvement such as transgenic operations; at the same time, there are many varieties of Camellia oleifera, and there are differences in the growth habits of different varieties, so the composition and treatment of the medium at each stage between different varieties are very different. Using the existing technology Breeding 'Huashuo' and other varieties selected in recent years cannot achieve the expected results, let alone meet the requirements of industrial seedling breeding

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