418results about How to "Increase the multiplication factor" patented technology

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial system are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplication rate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.

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The invention discloses a method for cultivating a subcultured bud of an upright crown tissue culture seedling of fir wood. The method comprises the following steps: an excellent clonal annual burgeon of the fir wood is used as an explant, a breeding bud is obtained through disinfection, sterilization and tissue culture, the breeding bud is innoculated in a proliferation culture medium comprising 1 / 2MS, 1.0mg / L of 6-BA and 0.5mg / L of IBA for inducing the cluster buds to sprout, the subcultured bud which is germinated at the base of the breeding bud and is healthy in growth is selected and is cut in a common rooting culture medium, seedlings are exercised and transplanted, and when the height of the seedlings of the fir wood is 15-30 cm, the seedlings are taken out of a garden for forestation. When the method is used, the proliferation rate of the subcultured bud of the fir wood is as high as 4-6, the breeding speed is high, the seedling culturing number is large, the tissue culture subcultured bud with high genetic stability, 100% of the upright crown rate of the tissue culture seedling and more than 96% of the rooting rate can be in batch production in a short period, and the requirements of the construction and the development of a fir wood reserving base of China for good seedlings can be met, so that the method has better economic benefits, social benefits and ecological benefits.

The invention discloses a tissue culture medium of sealwort roots and stems and an in-vitro regeneration method of the tissue culture medium. The tissue culture medium comprises an inducing culture medium, a proliferation culture medium and a rooting culture medium. The method using the tissue culture medium of the sealwort roots and stems for carrying out in-vitro regeneration on the sealwort roots and stems comprises the three steps of induction of sprouts, proliferation culture of the sprouts and rooting culture. The tissue culture medium and the propagation method have the beneficial effects that a system of in-vitro regeneration of the sealwort roots and stems is established by utilizing a tissue culture technology, and the inducing culture medium, the proliferation culture medium and the rooting culture medium in the method are selected and optimized; and the tissue culture medium has the advantages of high sprout induction rate, high proliferation coefficient, high rooting rate, strong sprouts and seedlings, normal leaf shape and color and the like, and can meet the need of the market for the sealwort roots and stems.

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

The invention discloses a method for propagating plants rapidly. The method comprises the following steps that butterfly orchid pedicle auxiliary buds after flowering are induced to form nutritional buds, and then the nutritional buds are cut, and the abandoned pedicle stem segments act as explants, and are directly inoculated into an induction culture medium for inducing clustered buds; the novel induced buds are cut off to be inoculated to the propagation culture medium for subculture propagation culture; finally rooting and strenghening are carried out and tissue culture seedlings are transplanted. By the method, the auxiliary buds on the butterfly orchid pedicles after flowering and abandoned pedicle materials after bud cutting germinate in a manually prepared culture medium for multiple times to generate novel buds. The method has the advantages that not only is the normal flower period of butterfly orchid not affected, but also butterfly orchid mother plants are not hurt, the butterfly orchid pedicle stem segments are recycled for 2-3 times; pedicle materials are used fully, waste is turned into wealth; the propagation velocity and the production of butterfly orchid tissue culture seedlings are improved greatly; the produced seed seedlings are strong and neat, and are convenient to manage in a factory.

The invention discloses a swordlike atractylodes rhizome tissue culture and rapid propagation method. By adopting the method, various problems such as varietal complexity caused by artificial cultivation and variety degeneration, serious diseases and yield reduction caused by long-time vegetative propagation can be solved. Plant tissue culture has the characteristics of rapid propagation, low cost and high multiplication coefficient; and according to the swordlike atractylodes rhizome tissue culture and rapid propagation method disclosed by the invention, stems with buds are taken as explants, a swordlike atractylodes rhizome tissue culture and rapid propagation system can be established by virtue of the steps such as induction, multiplication, rooting and acclimatization and transplanting, so that the propagation speed and scale of seedlings can be expanded, new swordlike atractylodes rhizome varieties with high yield and high quality can also be bred on the basis of expanding the propagation speed and scale of the seedlings, and the economic benefits of swordlike atractylodes rhizome planting can be increased; and the swordlike atractylodes rhizome tissue culture and rapid propagation method has important significance in solving the realistic problem of short supply of swordlike atractylodes rhizome and protecting wild swordlike atractylodes rhizome resources.

The invention discloses a rapid breeding method of lamina seedlings of tinospora capillipes gagnep. The method comprises the steps: (1) scratching the back sides of tender laminas of sterile test tube seedlings of tinospora capillipes gagnep to be well-shaped by using a scalpel and putting into an MS propagation medium for culturing; performing induction formation on callus and classifying to obtain multiple shoots; (2) putting the multiple shoots into an MS strong seedling medium for culturing strong seedlings to obtain strong plants; and (3) putting the strong plants into a 1 / 2 MS rooting medium to obtain complete root seedlings. The proliferation coefficient of the multiple shoots of the tinospora capillipes gagnep obtained by using the culturing method reaches 20-30 times, the rooting rate of the obtained tissue culture seedlings is more than 95 percent, the survival rate of seedbed transplanting is more than 90 percent, and the scale seedling breeding problem of the tinospora capillipes gagnep is effectively solved.

The invention relates to an orchid rapid-propagation culture medium formula. Culture mediums for fast propagation of orchids include starting culture mediums, proliferation culture mediums and rooting and seedling strengthening culture mediums which are used in three culture stages. According to the culture medium formula, no plant hormones are added, and the characteristic of wild imitation cultivation is achieved. Orchid tissue culture seedlings obtained through the formula are good in growth vigor, regular and high in transplantation survival rate.

The invention provides a method for rapidly reproducing protruding eucalyptus through tissue culture, which comprises the steps that: excellent tree bodies are selected as explants which are then collected and sterilized; initial culture, multiplication culture and rooting culture are carried to stem sections; then the seedlings adopt seedling adaptation, transplantation from bottles and seedling management. The method of the invention can increase multiplication coefficient of 3 to 5. The inheritable characters of the germchit of the protruding eucalyptus are stable, and the germchit of the protruding eucalyptus reaches improved variety and cloning, thus increasing the wood yield of an elemental area and promoting the ecological and economic benefits of the woods of the protruding eucalyptus.

The invention discloses a Eucalyptus dunnii group culture fast propagation rootage method, which comprises the following steps: (1) asepsis seedling cultivation; (2) bud subculture multiplication; (3) test-tube seedling rejuvenation; (4) inducing rootage; (5) transplanting the test-tube seedling, etc. In the invention, after the multiplication cultivation and rejuvenation cultivation, the asepsis seedling is inoculated in a first rootage culture medium and a second rootage culture medium for inducing a complete plant, thus solving the problem that the propagation coefficient of Eucalyptus dunnii group culture is low, in particular to the problem that the rootage is difficult; by utilizing the technical proposal of the invention, a large number of seedlings can be provided in a short time so as to replace the expensive imported seeds and satisfy the heavy demand of the large-scaled industrial seedling and the market.

The invention relates to a tissue culture rapid-propagation method of beaucarnea recurvata. The tissue culture rapid-propagation method comprises the following steps: (1) placing a disinfected beaucarnea recurvata seed as an explant into an MS germination culture medium for germination induction to obtain a sterile test-tube seedling; (2) placing the test-tube seedling into an MS cluster bud culture medium for propagation culture to obtain cluster buds; (3) placing the cluster buds into an MS strong seedling culture medium for culture to obtain a strong plant; (4) placing the strong plant into a 1 / 2MS rooting culture medium for culture to obtain a rooting seedling; (5) carrying out seedling adaptation on the rooting seedling, and transplanting to a sand bed to grow for one month, and then transplanting to a field. According to the tissue culture rapid-propagation method, the multiplication coefficient of the obtained cluster buds is 20-30 times, the rooting rate of an obtained tissue culture seedling is more than 95%, and the survival rate of a transplanting seedbed is more than 90%, so that the problem of industrialized seedling culture of the beaucarnea recurvata is effectively solved.

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

The invention discloses a method for tissue rapid cultivation of Acer rubrum. The method comprises the following steps: (1) conducting surface sterilization by adopting tender stem segments of Acer rubrum as explants; (2) cultivating on an initial medium for 25-30 days; (3) cultivating on a multiplying medium for 20-40 days; (4) conducting rooting cultivation on a rooting medium till generating appropriate roots. By adopting the method, excellent aseptic seedlings of Acer rubrum can be rapidly and constantly acquired, the survival rate of seedlings is high, technical guarantee is provided and solid foundation is established for industrialized seedling production of Acer rubrum, and industrialized development of Acer rubrum cultivation in China is facilitated.

The invention discloses a wild imitation cultivation method of dendrobium officinale tissue culture seedlings. The method comprises the following steps: selecting seedlings, airing seedlings, cutting boards, planting seedlings, fixing roots and performing transplanting. After the seedlings are selected, cleaned and aired, the seedlings are directly fixedly planted and bound on the cut boards with barks; then the seedlings are suspended collectively in a domestication greenhouse for domestication; and after the roots of the dendrobium officinale tissue culture seedlings are fixed, the domesticated dendrobium officinale tissue culture seedlings and the boards with barks are transplanted to a suitable field environment for wild imitation growth. The method leaves out a step that greenhouse seedbed domestication must be performed on tissue culture seedlings in traditional methods, reduces the production processes, saves a large amount of investment of manpower, material resources and financial resources, greatly reduces the production cost, and shortens the production time. The tissue culture seedlings cultivated by the wild imitation cultivation method has a high survival rate and wide transplanting range, and the method is simple and convenient, has high operability, is good in safety and reliability, and is high in survival rate, and the post-seedling phenomenon is greatly reduced.

The invention discloses a induction method of Brown Lily bulblet in vitro, including the following steps: (1)Brown Lily bulblet scale without pest is selected, then it is washed with tap water, dipped into 75% alcohol for 30 seconds, washed with sterile water, dipped into 3% sodium hypochlorite for 10 minutes, shaked for a while, washed with sterile water 6-7 times, then placed into culture dish containing asepsis filter paper and cut into blockes for reservation; (2) the base part of the scale is inoculated onto the induction medium for culture, the culture temperature is 23-27 DEG C, the light intensity is 1500Lx, the light time is 12 hours per day; (3) after 30-day induction culture, the grown bulblet scale is placed onto multiplication medium for multiplication culture, when the diameter of the bulblet scale is up to 1-2 cm, it is transplanted onto dish containing disinfected substrate, then watered and covered with fresh-keeping film, after 3-5 days it is ventilated, when new leaves are grown up on the tube seedling, the fresh-keeping film is removed. The invention can shorten the emerging time and reduce cost with simple operation.

The invention discloses a rapid propagation method for holcoglossum flavescens. Rapid propagation of holcoglossum flavescens is completed through the steps of seed germination, induction of protocorm, differentiation culture, strong seedling culture, rooting culture, acclimatization and transplant and the like. Compared with a traditional division method, the growth coefficient of holcoglossum flavescens is improved by more than 5 times, the planting percent reaches 95%, the survival rate of transplanting reaches more than 85%, meanwhile, the method has the advantages of being large in increment coefficient, strong in tillering capacity, high in growth speed, strong in plant, low in browning ratio, high in survival rate of transplanting, and low in cost, culture medium is easy to prepare, and the method can be applied to large scale industrial production.

The invention discloses an in-vitro rapid propagation method of cercidiphyllum japonicum. The cercidiphyllum japonicum is the valuable, rare and endangered plant of secondary protection of China, and has extremely high scientific research, and medicinal and ornamental values. At present, the cercidiphyllum japonicum is generally propagated by cottage and grafting and by use of seeds, but due to the propagation methods, the reproduction rate is low and the reproduction period is long, and far fail in meeting the requirements of seedlings for landscaping, scientific research and the like; according to the in-vitro rapid propagation method of the cercidiphyllum japonicum, the in-vitro rapid propagation system of the cercidiphyllum japonicum is established by taking the stem with axillary buds of the cercidiphyllum japonicum as explant and by virtue of processes of explant processing, induced culture, propagation, rooting, acclimatization and transplanting and the like; the propagation coefficient of the cercidiphyllum japonicum can be increased, and then the industrialized seedling production of the cercidiphyllum japonicum can be realized.

The invention relates to a Rhododendron bachii Levl tissue culturing and rapid propagation method. The method comprises the steps of explant selection, explant disinfection, axillary bud induced culture, enrichment culture, strong seedling culture and ex vitro rooting. The method treating an annual blossom immature stem segment as the explant and WPM or Anderson as a basal culture medium and utilizing a ZT hormone has the advantages of good induction effect, high propagation coefficient, good cultivated seedlings, and fast rooting and survival, and allows an effective Rhododendron bachii Levl tissue culturing and rapid propagation system to be successfully established. In the invention, the propagation coefficient within a propagation subculture period (6-8 weeks) is controlled in a range of 5-6 times, and the propagation coefficient within one year is about 10000 times, so the propagation coefficient of Rhododendron bachii Levl is greatly improved; and the method is a reliable and effective propagation method for the preservation and sustainable development of the above species and provides a scientific technological support for the landscaping of wild rhododendrons.

The invention discloses a momordica grosvenori seedling growing method, and relates to the technical field of plant seedling growing. The method comprises: plowing a field, arranging a seedbed, establishing a sunshade, preparing seedling growing soil and loading the seedling growing soil to a nutrition pot, cutting a branch into a cutting with one leaf bud, inserting the cutting to the seedling growing soil inside the nutrition pot, placing the nutrition pot on the seedbed, spraying water and keeping the moisture at a level ranging from 70 % to 80%, and spraying organic nutrition liquid once per week. The seedling begins rooting after 30 days, and the seedling is transplanted when more than 3 pieces of true leaves grow out. Compared with prior art, the momordica grosvenori seedling growing method can exploit full bio-potential of the cutting so as to facilitate fast rooting. The cultivated seedlings are characterized by a strong root, rapid growth,tidy seedling emergence and a high adult seedling rate.

The invention discloses a phyllostachys pubescens tissue culture method. Phyllostachys pubescens is also called as mao bamboo, belongs to gramineous phyllostachys plants, is a bamboo species which integrates economic benefits, ecological functions and ornamental values, and has the characteristics of rapidness in growth, strong propagation, early maturing, wide application, low investment and large profit; and tissue culture seedling raising has the characteristics of rapid propagation, less cost and high multiplication coefficient; and according to the phyllostachys pubescens tissue culture method disclosed by the invention, phyllostachys pubescens seeds are taken as explants, a phyllostachys pubescens tissue culture rapid propagation technology system is established by virtue of steps such as seed disinfection, induction, multiplication, rooting and acclimatization and transplanting, and the phyllostachys pubescens tissue culture rapid propagation technology system can be used for providing technological support for further industrialized seedling raising of gramineous phyllostachys tissue culture, and can also be used for laying a foundation for genetic transformation and species improvement of phyllostachys pubescens.

The invention relates to a tissue culture method for rapidly propagating wild lilies and belongs to the field of a flower culture technology. According to the method, bud-stage flower stems of the lilies are used as explants of tissue culture of the lilies and are completely disinfected before culture; besides that 6-BA (Benayl Aminopurine), NAA (Naphthalene Acetic Acid) and IBA (Indole Butyric Acid) are used as auxins or hormones in a culture medium, an MES (Methyl Ester Sulfonate) is added to be used as a buffering agent so as to effectively control the pH value in a tissue culture process, and the pH value is maintained in the optimal range of differentiation, propagation and growth of the lily explants; therefore, the induction efficiency and the propagation coefficient are improved, the induction rate is 98%-100%, the quantity of adventitious buds induced by each explant is not less than 15, the propagation coefficient is 5-7, the rooting rate is 100%, and the root system is healthy and strong. The tissue culture method is applicable to safe propagation of good single plants of filial generations and is an effective manner for protecting wild resources of lilies.

The invention relates to a tissue culture rapid-multiplying method for bletilla striata protocorm-like bodies. The method comprises the following steps: (1) slitting bletilla striata and sterile test-tube plantlets into single corms with plantlets, inoculating to an MS protocorm-like body multiplying culture medium for multiplying culture to obtain protocorm-like bodies; (2) putting the obtained protocorm-like bodies into an MS rejuvenation culture medium for rejuvenation culture to obtain protocorms with seedlings; and (3) inoculating the protocorms with seedlings onto a 1 / 2 MS rooting culture medium for rooting culture to obtain small robust plants. The bletilla striata protocorm-like bodies obtained by adopting the tissue culture rapid-multiplying method are high in rejuvenation coefficient, short in rejuvenation time, robust for the rejuvenated multiple buds, the high-quality bletilla striata seedlings with high survival rate can be provided within short period, and the large-scale seedling culture problem of the bletilla striata can be effectively solved.

The invention belongs to the technical field of plant tissue culture and discloses a dendrobenthemia japonica tissue culture method. The dendrobenthemia japonica tissue culture method comprises the steps of pretreating, performing primary culture, enrichment culture and strong seedling culture, planting in a rooting medium and transplanting, wherein in the culturing process, the external conditions are as follows: pH is 5.8-6.0, the culture temperature is 23-25 DEG C, the illumination period is 8-10h / d, and the illumination intensity is 1800-2000lux. The culture method has the benefits that 1. with the adoption of the culture method, a growth coefficient is high, a rooting rate is high, and a cultured plant has excellent growth characteristic; and 2. large scale production can be achieved by the culture method, and the reproduction efficiency is high.

The invention discloses a quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis, which comprises the following steps of the selection and disinfection of explants, induction culture, multiplication culture, root induction culture and transplantation rooting. In the tissue culture propagation method for the narcissus tazetta var. chinensis, the complete technical process from the selection of the explants to the transplantation of the explants is accomplished, and bulbs subjected to the multiplication culture are rooted and induced within short time and are transplanted to a perlite substrate for rooting directly, so that test tube seedlings of the narcissus tazetta var. chinensis are not needed to be rooted in vitro; and the steps of induction rooting and transplantation are simplified, the production period is shortened, the cost is reduced, and the survival rate of the transplantation reaches over 92 percent. The method is high in pertinence, simple and easy and high in multiplication rate, produced seed bulbs are high in consistency and few in plant diseases and insect pests, the cost is saved, and the requirements for the industrial production of the narcissus tazetta var. chinensis seedlings can be met.

The invention relates to a populus alba * pobulus davidiana dode 1333 tissue culture medium of an pobulus davidiana dode improved variety, which is used for solving a problem that the existing culture medium is poor in in-vitro rapid propagation effect for populus alba* pobulus davidiana dode 1333 regeneration tissue of hybrid offspring pobulus davidiana dode improved variety of pobulus davidiana dode and populus alba. The populus alba* pobulus davidiana dode 1333 tissue culture medium of the pobulus davidiana dode improved variety comprises an adventitious bud differentiation culture medium, a subculture multiplication culture medium and an adventitious bud rooting culture medium, wherein the adventitious bud differentiation culture medium consists of a YS (Eosine Yellowish) culture medium, 6-benzyl amido purine and kinetin; the subculture multiplication culture medium consists of the YS culture medium, the 6-benzyl amido purine and naphthylacetic acid; the adventitious bud rooting culture medium is composed of the YS culture medium and hormone A, wherein the hormone A consists of one or several of indolebutyric acid, indoleacetic acid and naphthylacetic acid in proportion. The populus alba * pobulus davidiana dode 1333 tissue culture medium disclosed by the invention is applied to the plant tissue culture filed.

The invention discloses a one-step seedling method for rapid propagation of pseudobulbe of phaius tankervilleae. The one-step seedling method includes transplanting original corm of phaius tankervilleae differentiating the root and bud structure to a multiplication culture medium for subculture, culturing for 60-70 days to obtain multiplication seedlings with roots and buds and clustered peudobulbe, and keeping subculturing the multiplication seedlings for 20-30 days to obtain test-tube plantlets with buds 3-6 cm high and roots 1-2 cm long for acclimatizing and transplanting. The formula of the multiplication culture medium comprises 1.0-2.0 mg / L of MS, 6-BA, 0.5-1.0 mg / L of KT, 0.2-0.6 mg / L of TDZ, 0.5-1.5 mg / L of NAA, 0.1-0.2% of activated carbon, and 10-20% of coconut juice. PH is 6.0. The one-step seedling method can realize multiplication and rooting of phaius tankervilleae by subculture multiplication, and can culture at one step by omitting rooting and culturing. In addition, the one-step seedling method is short in culture period, high in multiplication coefficient and low in production cost.

The invention discloses a breeding method of tissue-culture seedlings of Honghua kiwi fruits. The breeding method comprises the following steps: adopting stem sections drawn in the current year as explants, after explants are disinfected, inoculating the explants on an induction culture medium to carry out axillary bud induction culture to obtain axillary buds; cutting the obtained primary axillary buds into single buds, transferring into a secondary culture medium for secondary culture to obtain secondary cluster buds; when the secondary cluster buds grow to be 1.5-2cm in height, cutting thecluster buds into single buds, transferring into a rooting culture medium to carry out rooting culture, thus obtaining test tube seedlings; and transplanting the obtained test tube seedlings into a mixed medium with the volume ratio of perlite to peat soil being 1:9 to obtain the tissue-culture seedlings of the Honghua kiwi fruits. The breeding method disclosed by the invention has the advantagesof high axillary bud induction rate, high adventitious bud proliferation coefficient, high rooting rate, high survival rate of transplantation of test tube seedlings, strong growth of bud seedlings, normal shapes and colors of leaves, subcutaneous rooting and fast germination of new leaves and the like, and can meet the need of large-area and large-scale cultivation of the Honghua kiwi fruits.

The invention belongs to the field of plant tissue culturing, and in particular relates to a method for promoting rapid propagation of cluster buds of a butterfly orchid by utilizing an LED (Light Emitting Diode) light source. The method comprises the following steps: (1) inducing the generation of cluster buds by taking a pedicel of the butterfly orchid as an explant; (2) cutting the cluster buds obtained in the step (1) into simple buds or bunch buds and inoculating the simple buds or the bunch buds into an enrichment medium; and (3) by taking an LED capable of emitting red light or emitting warm white light as a light source, culturing the cluster buds which are obtained in the step (2) and are inoculated into the enrichment medium under the LED capable of emitting red light or emitting warm white light. According to the method, the number of the single buds of the cluster buds is obviously increased; the dry weight, the fresh weight and the plant height of the cluster buds are all increased; and the production cost is lowered and energy conservation is realized.