The invention discloses a rapid propagation method for holcoglossum flavescens. Rapid propagation of holcoglossum flavescens is completed through the steps of seed germination, induction of protocorm, differentiation culture, strong seedling culture, rooting culture, acclimatization and transplant and the like. Compared with a traditional division method, the growth coefficient of holcoglossum flavescens is improved by more than 5 times, the planting percent reaches 95%, the survival rate of transplanting reaches more than 85%, meanwhile, the method has the advantages of being large in increment coefficient, strong in tillering capacity, high in growth speed, strong in plant, low in browning ratio, high in survival rate of transplanting, and low in cost, culture medium is easy to prepare, and the method can be applied to large scale industrial production.