7results about How to "Increase the multiplication factor" patented technology

This invention relates to the field of plant tissue culture, specifically disclosing a method for rapidly propagating dandelion through tissue culture. The method includes the following steps: inducing callus tissue using dandelion leaves as explants; inducing shoot formation from the callus tissue; and rooting the shoots to obtain dandelion tissue culture seedlings. This invention provides a high-quality and efficient tissue culture propagation method for dandelion by utilizing dandelion leaves as explants, inducing callus tissue through dedifferentiation, allowing the callus tissue to redifferentiate and produce shoots, and then rooting individual shoots.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

The present application relates to the technical field of plant tissue culture, and provides a tissue culture method for promoting germination of Zingiber zerumbet Smith, wherein Zingiber zerumbet Smith tubers are used as explants, and after being subjected to disinfection treatment, the explants are inoculated into an induction medium, a proliferation medium and a rooting medium in sequence, and then are subjected to acclimatization, seedling raising and transplanting, so that finished Zingiber zerumbet Smith seedlings are obtained. The germination rate, the proliferation coefficient and the rooting rate of the Zingiber zerumbet Smith tubers can be significantly improved by using the culture media which are optimized and adjusted in the technical scheme of the present application. In addition, the reasonable application of foliar fertilizer and fungicide after transplantation in the present application can further improve the growth vigor and the disease resistance of Zingiber zerumbet Smith seedlings, and ensure the survival rate of the seedlings, thereby providing reliable technical support for rapid propagation and large-scale planting of Zingiber zerumbet Smith.

The application discloses a tissue culture large-scale propagation method of Camellia meiocarpa. The method comprises the following steps: obtaining aseptic seedlings of Camellia meiocarpa, cluster bud proliferation culture, single plantlet culture of adventitious buds, bottle-out root preparation culture and bottle-out root culture. The composition of the cluster bud proliferation culture medium and the plantlet culture medium is optimized, the cluster bud proliferation and the formation of the adventitious buds are promoted, and the proliferation coefficient reaches 8.18. In addition, the bottle-in and bottle-out root culture scheme is combined, so that the rooting rate and the survival rate are significantly improved, and the rooting rate reaches 90%. The tissue culture method has important significance in the protection and population recovery of Camellia meiocarpa, and provides technical support for the protection and sustainable utilization of the endangered species.

This invention belongs to the field of tissue culture technology and provides a method for rapid propagation of Tibetan Saussurea involucrata via tissue culture, comprising the following steps: From May to July, Tibetan Saussurea involucrata explants are subjected to stepwise sterilization to obtain sterile explants; these explants are inoculated into a starter culture medium for seed germination to obtain callus tissue; the callus tissue is transferred to MS proliferation medium containing 2.0-3.0 mg / L 6-BA + 0.05-0.1 mg / L IAA, and cultured for 21-25 days. TDZ is then added to promote shoot differentiation, while simultaneously reducing IAA to 0.01-0.02 mg / L, and cultured for another 21-25 days to obtain rootless seedlings; these seedlings are then transferred to rooting medium and cultured for 21-25 days to obtain tissue culture seedlings; after hardening off the seedlings for 2-4 days, they are transplanted into a sterile substrate. This method significantly improves the propagation stability and seedling quality of Tibetan Saussurea involucrata via tissue culture by quantifying key process parameters and establishing a multi-dimensional evaluation system.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.