Tissue culture and rapid propagation method and culture medium for apple continuous cropping-resistant dwarf rootstock G41

Abstract

The invention discloses a tissue culture and rapid propagation method and culture medium for an apple continuous cropping-resistant dwarf rootstock G41; the culture medium contains a startup culture medium, a proliferation culture medium and a rooting culture medium, wherein the startup culture medium comprises the components: MS, 6-BA, IBA, NAA, sucrose and agar; the proliferation culture mediumcomprises the components: MS, 6-BA, IBA, sucrose and agar; the rooting culture medium comprises the components: 1 / 2MS, IAA, IBA, sucrose and agar. Through the startup culture medium and the proliferation culture medium, a stem apex body is subjected to tissue culture treatment; through the rooting culture medium, tissue culture seedlings are subjected to rooting treatment, propagation of cutting and layering are no longer carried out, and the survival rate of transplanted seedlings is increased.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation method and a culture medium, in particular to a tissue culture and rapid propagation method and a culture medium for an apple resistant to heavy cropping dwarf rootstock G41. Background technique [0002] Intensive cultivation of dwarf stock is the main mode. How to overcome the obstacle of heavy cropping in new orchards has become a major problem to be solved urgently in the development of apple industry. In addition to agronomic measures such as crop rotation, deep plowing, and foreign soil, the application of dwarf rootstocks resistant to heavy cropping is the most important. Simple and effective way, there are two existing apple dwarf rootstock breeding methods: cutting and layering. For apple rootstock G41, which is resistant to heavy cropping, cutting is not easy to take root and difficult to grow seedlings, and the demand for cuttings is large, and the breeding coefficient Although ...

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Problems solved by technology

[0002] Intensive cultivation of dwarf stock is the main mode. How to overcome the obstacle of heavy cropping in new orchards has become a major problem to be solved urgently in the development of apple industry. In addition to agronomic measures such as crop rotation, deep plowing, and foreign soil, the application of dwarf rootstocks resistant to heavy cropping is the most important. Simple and effective way, there are two existing apple dwarf rootstock breeding methods: cutting and layering. For apple rootstock G41, which is resistant to heavy cropping, cutting is not easy to take root and difficult to grow seedlings, and the demand for cuttings is large, and the breeding coefficient Although layering can produce seedlings, it needs multiple cultivations in the breeding process, which requires the cultivation height and material ratio. It is also easy to cause damage to the root system of seedlings when raising seedlings by division, affecting the survival of slow seedlings, and the reproduction coefficient is relatively low. Low

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