82 results about "Carassius auratus gibelio" patented technology

The invention relates to a method for comprehensively raising various types of aquatic livestock and cultivating paddy in a paddy field, in particular to the raising of Chinese soft-shelled turtles, crawfish and carassius auratus gibelio in the paddy field and the cultivation of paddy. The method comprises the steps of paddy field preparation, aquatic plant transplantation, live food feeding, paddy transplantation, young aquatic livestock placement, bait feeding, daily management, aquatic livestock fishing and paddy harvest. The method for comprehensively raising various types of aquatic livestock and cultivating paddy in the paddy field has the advantages that the various types of aquatic livestock can be raised and paddy can be cultivated in the same field comprehensively, the difference between the period for paddy cultivation and the period for raising aquatic livestock is small, the use rate and the comprehensive efficiency of the field can be effectively improved, and economic benefit can be improved to a large extent; meanwhile, any chemical fertilizer, herbicide and insecticide are not used in the process of production, the produced products can meet the standard of organic food, and finally, edible safety of the products is achieved; the whole process of production is beneficial to conservation of water and soil of the field and the surrounding environment and effectively facilitates the protection of natural ecology.

The invention relates to a method for optimal seedling selection and breeding of novel carassius auratus gibelio series, which comprises the following steps: (1) extracting an egg of a Fangzheng crucian carp; (2) extracting a sperm of a Xingguo red carp; (3) stimulating the female nucleus of the egg of the crucian carps for development to generate an offspring through the sperm of the Xingguo red carp; (3) extracting a sperm of a grass carp and a sperm of a megalobrama amblycephala, and extracting an egg of the offspring generated in the previous step; (5) stimulating the female nucleus of the egg of the offspring generated Step (3) for development with the sperm of the grass carp and the sperm of the megalobrama amblycephala; and (6) breeding the improved seedling. The invention has the following technical effects that: 1) the novel carassius auratus gibelio series with genetic stability can be obtained; 2) the sporozoon resistance of the bred novel carassius auratus gibelio series can be significantly improved; 3) the growth speed of the bred novel carassius auratus gibelio series is high, and the intestinal length/full length is increased, thereby facilitating the improvement of the digestibility; and 4) the thickness of the dorsal muscles and the tightness of the scales of the bred novel carassius auratus gibelio series are improved, the survival rate in the long-distance transport is more than 95 percent, the visceral mass is reduced, the body length is increased, and the intramuscular bones are decreased.

The present invention discloses a compound feed for carassius auratus gibelio, and belongs to the field of aquaculture feed. The compound feed comprises the following components, by mass, 13-16% of fish meal, 3.5-5% of meat and bone meal, 5.0-8.5% of blood meal, 12-16% of rapeseed meal, 24-28% of soybean meal, 21-24% of wheat flour, 7-10% of rice bran, 1-2% of soybean oil, 1-2% of calcium dihydrogen phosphate, 0.2-0.4% of choline chloride, 0.01-0.03% of taurine, 0.01-0.03% of bile acid and 1-3% of a premix. The compound feed has the following advantages that: the formulations of the compound feed is reasonable; the price is low; the uptake of the energy and the uptake of the protein are both covered; the adequate uptakes of the essential amino acids are ensured; the bile acid and the taurine are added to regulate the body constitution of the carassius auratus gibelio, promote the emulsification of the fat, increase the activity of the lipase, such that the fat can be effectively absorbed, and the utilization efficiency of the energy is improved.

The invention relates to carassius auratus gibelio anti-stress feed and a preparation method thereof, belonging to the technical field of compound feed for aquatic products. The preparation method comprises the following steps: stirring and uniformly mixing fish meal, soybean meal, blood meal, rapeseed meal, cottonseed meal, bran, wheat shorts, rice bran, crushed potassium sorbate mildew inhibitor, fish oil, soybean lecithin powder, 50% of choline chloride, curcumin, premix for fish, attapulgite, 50% of vitamin E and calcium dihydrogen phosphate; crushing by an ultramicro-crushing machine to form a mixture after stirring and uniform mixing, and finally preparing the granular feed by utilizing a conventional method of the granular feed. The granular feed disclosed by the invention has comprehensive and rich nutrition, and can improve immunity and oxidation resistance of carassius auratus gibelio, improve stress, enhance disease resistance, improve palatability of the feed and promote growth.

The invention relates to a liver and gallbladder protective pellet feed for carassius auratus gibelio and a production method of the liver and gallbladder protective pellet feed, and belongs to the technical field of aquatic feeds. The production method comprises the following steps of: crushing fish meal, soya bean meal, rapeseed meal, peanut meal, corn gluten meal, cottonseed meal, flour, rice husk and bran; stirring and uniformly mixing the crushed materials with mixed oil, monocalcium phosphate, enzymic preparations, allicin, 50% choline chloride, curcumin, potassium sorbate, table salt and premix; crushing again through a crusher to obtain a mixed material after stirring and uniformly mixing; and finally preparing the mixed material into the pellet feed through the conventional method of the pellet feed. The liver and gallbladder protective pellet feed has comprehensive and rich nutrition, can be used for improving the immunocompetence of the carassius auratus gibelio, enhancing the disease resistance, improving the feed palatability and increasing the feed utilization rate, has the liver and gallbladder protective functions, thereby being capable of promoting the fish body to grow healthily.

The present invention relates to a carassius auratus gibelio anti-stress puffing feed and a production method thereof, and belongs to the technical field of aquatic feeds. The production method includes crushing fish meal, soybean meal, rapeseed meal, peanut meal, corn gluten meal, cottonseed meal, wheat, flour, rice bran, and wheat bran; mixing with oil, monocalcium phosphate, coated lysine, coated methionine, anthraquinone extract, 35% phosphate vitamin C, 50% vitamin E, yeast culture, a potassium sorbate preservative, salt, and a premix uniformly; grinding into a mixed material by an ultrafine grinder after mixing, placing the mixed material into a regulator, feeding water vapor for regulation, and preparing into a pellet feed by a extrusion granulation machine. The carassius auratus gibelio anti-stress puffing feed is comprehensive and rich in nutrition, and can improve immune and antioxidant capacity of carassius auratus gibelio, improve anti stress ability, enhance disease resistance, reduce the water pollution in breeding process caused by feeding, improve palatability of the feed, and promote growth.

At present, the total artificial breeding technology is generally used for breeding Carassius aurutus gibelio in our country, which has the main following defects due to adopting an artificial insemination method: influenced parent survival rate, low fertility rate, hatchability and increased offspring seed cost. In the semi-artificial breeding technology for Carassius aurutus gibelio in the invention, the artificial insemination in the complete artificial breeding process is changed into natural insemination, namely, a natural breeding environment for the Carassius aurutus gibelio is simulated in a pond so as to enable the male and female parents to perform copulation and breeding. The invention has the following advantages: the pond is utilized to create the natural breeding environment for the Carassius aurutus gibelio so as to improve the fertility rate which can reach above 95% generally; and natural copulation between the female and male parents is finished in the pond, thus avoiding parents from being injured, improving the parent survival rate, saving a great amount of manpower and material resources and effectively enhancing the breeding efficiency. Therefore, the semi-artificial breeding technology for the Carassius aurutus gibelio in the invention can solve the problem of breeding the Carassius aurutus gibelio in a scale.

The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line of Carassius auratus gibelio and establishing method and use thereof. The cell line is deposited in China Center for Type Culture Collection under an accession number of CCTCC No: C2013179. The brain tissue cell line of Carassius auratus gibelio is in good growth state and sensitive to CyHV-2 that is presently hardly cultured with ordinary fish cell lines. After six passages of CyHV-2 in GiCB cells, viral nucleic acid can still be detected and a cytopathic effect is stable. When ultrathin microscopic sections are prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and their replication process can be observed in GiCB cells. The construction method of the brain tissue cell line of Carassius auratus gibelio of the present invention has high repeatability, scientific and reasonable conditions.

The invention discloses a method for breeding and cultivating four generations of fish and shrimp seedlings in a pond in one year. The method comprises the steps that first, parents are selected; second, pretreatment is conducted on the pond; third, the fish and shrimp seedlings are bred and cultivated. According to the breeding time and method, the first generation of the seedlings is obtained by breeding carassius auratus gibelio seedlings from April first to May fourth; the second generation of the seedlings is obtained by breeding chub, bighead and grass carp seedlings from May fifth to June sixth; the third generation of the seedlings is freshwater shrimp little specification seedlings, and the freshwater shrimp little specification seedlings are bred from June fifth to July twentieth; the fourth generation of the seedlings is freshwater shrimp large specification seedlings, and the freshwater shrimp large specification seedlings are bred from July twenty-first to December thirtieth. The overflow seedling breeding process comprises the steps that high-quality fish and shrimp seedlings are selected and bred in the overflow selection and breeding mode respectively for fish and shrimp seedlings which have already been bred and cultivated; proper fodders are fed for the fish and shrimp seedlings; fourth, water quality is adjusted and controlled; fifth, biological agents are properly used in the high-temperature season. The method for breeding and cultivating the four generations of the fish and shrimp seedlings in the pond in one year has the advantages that the four generations of high-quality fish and shrimp seedlings can be bred and cultivated in the pond in one year, the pond utilization rate is improved by one time, and economical benefits are improved by one time.

The invention relates to special compound feed for carassius auratus gibelio and a preparation method of the compound feed. The compound feed is prepared by the following raw materials by weight percentage: 20-25% of soybean meal, 15-20% of rapeseed meal, 10-15% of cottonseed meal, 5-10% of fish meal, 20-30% of wheat middling, 3-6% of fish oil, 5-15% of bran, 1% of microelement additive for fish, 0.3% of decavitamin for the fish and 0.7% of special Chinese herbal medicine feed additive for the carassius auratus gibelio. With the adoption of the special compound feed for the carassius auratus gibelio, economic benefits of carassius auratus gibelio culture can be improved obviously; the compound feed can significantly reduce the disease incidence during the carassius auratus gibelio culture, particularly the incidence of an explosive hemorrhagic disease; a survival rate is increased by 11-13%; growth of the carassius auratus gibelio is promoted; the quality of the carassius auratus gibelio can be improved; and the cost of the raw materials used in the compound feed, the production cost and the use cost are lower, so that cost saving is facilitated.

The invention relates to a cyprinid herpesvirus II DNA vaccine based on a baculovirus vector as well as a building method and application thereof. Codons of regional sequences 1-186, 993-1197, 603-783and 85-186 nt of cyprinid herpesvirus II ORF72, ORF66, ORF81 and ORF82 are optimized; then, ORF72-ORF66-ORF81-ORF82 fusion sequences are chemically synthesized; next, the promoter control is performed; the clone into the baculovirus transfer vector pFSATBacTMDual is performed to build recombinant plasmids pFSATBacTMDual-FA-D4ORF; after the plasmids are used for converting DH10/Bac competent cells, Bacmid-FA-D4ORF is obtained; after the Bacmid-FA-D4ORF DNA is used for transfecting silkworm culture cells, BmNPV-FA-D4ORF is obtained and is the cyprinid herpesvirus II DNA vaccine based on the baculovirus vector. The DNA vaccine can realize the immune on carassius auratus gibelio by using an injection or oral administration or soaking method; the gill bleeding diseases of the carassius auratusgibelio can be reduced.

The invention provides a compound feed for preventing and treating gills bleeding of carassius auratus gibelio. The compound feed is prepared from the following components in percentage by weight: 5%-10% of fish meal, 1%-15% of tenebrio molitor flour, 0.2%-10% of ginseng, 0.2%-5% of radix achyranthis bidentatae, 0.2%-5% of mushroom, 0%-10% of acanthopanax root, 0.2%-10% of herba epimedii, 0.2%-5% of liquorice, 20%-30% of cottonseed meal, 0%-5% of rapeseed meal, 5%-10% of bean pulp, 15%-20% of peanut meal, 5%-15% of soya-bean oil, 15%-20% of wheat, 0.2%-0.5% of choline chloride, 2% of monocalcium phosphate and 1.5% of gunk. Long-term breeding proves that the compound feed for preventing and treating gills bleeding of carassius auratus gibelio, prepared from an insect protein source and Chinese medicine raw materials as feed raw materials, is provided for the first time; and the effect of reducing gills bleeding is significant, thus the survival rate of breeding is significantly increased; and the compound feed is high in pertinence, good in effect, obvious in economic value and worthy of large-range popularization and application.

The invention relates to a low fishmeal feed for the final grow-out period of carassius auratus gibelio and a preparation method thereof, and belongs to the technical field of aquatic formula feeds. The preparation method comprises the following steps: grinding fish meal, blood powder, soybean meal, rapeseed meal, cottonseed meal, flaxseed cake, sesame seed meal, bran, flour, rice bran, vegetable oil, premix for fish, bile salt, 50% choline chloride, attapulgite, non-starch polysaccharide enzyme, lysine, methionine, forsythia fructus extract, monocalcium phosphate and potassium sorbate mildew preventive, uniformly stirring and mixing the powders; grinding the mixture into a mixed material by virtue of a grinder; and finally preparing pelletized feed by utilizing a conventional pelletized feed method. The low fishmeal feed for the final grow-out period of carassius auratus gibelio is comprehensive and rich in nutrition, and can be used for improving the immunity of carassius auratus gibelio, improving stress, reinforcing disease resistance, improving feed palatability and promoting growth.

The invention discloses spinal cord tissue cell lines of Carassius auratus gibelio and a construction method and application thereof. The class name of the cell lines is Spinal cord tissue cell linesof Carassius auratus gibelio (CSC), and the collection number is CCTCC NO: C2018211. The above cell lines can be applied to separation, culture and detection of the Herpesvirus cyprini Type II and preparation of a Herpesvirus cyprini Type II vaccine. The invention provides a necessary technical platform for the separation and identification of CyHV-2 and its complete biological characteristics, and lays an important foundation for the prevention and control of the hematopoietic necrosis of the carp.

The invention discloses environment friendly carassius auratus gibelio fodder with little fish meal. The fodder comprises the following raw materials in parts by weight: 6-10 parts of steam fish meal with the concentration of 65%, 12-17 parts of dehulled soybean meal with the concentration of 46%, 1-3 parts of rupture blood corpuscle powder, 3-4 parts of pork powder, 15-20 parts of cottonseed meal with the concentration of 46%, 15-20 parts of Canadian rapeseed meal, 10-12 parts of flour, 5-8 parts of fine bran, 10 parts of full fat rice bran, 1-2 parts of soybean oil, 1-2 parts of lard oil, 2-3 parts of monocalcium phosphate, 1-2 parts of bentonite, 1-2 parts of zeolite powder, 0.2 part of choline chloride, 0.2 part of salt, 0.04 part of a compound microbial ecological agent, 0.05 part of vitamin C, 0.01 part of allicin, 0.5 part of decavitamin and 0.5 part of compound mineral salt. According to the method, the leftover materials of slaughter houses, namely, blood corpuscle powder and meat powder, are adopted as raw materials to replace fish meal, and the raw material resources are rich; by rationally utilizing the raw materials, the fish meal dosage is decreased, the environmental protection is benefited, the breeding cost is reduced, and breeding profits are increased; through repeated argumentation in actual production, the fish meal dosage is decreased by half, the dependency of fodder for fish meal is decreased, the environmental stress is decreased, the cost is reduced by 10 %, the income of breeders is increased with the output effect not changed.

The invention relates to a Carassius auratus gibelio anti-Cyprinid herpesvirus 2 (CyHV-2) oral recombinant Bacillus subtilis spore vaccine and a preparation method thereof. The vaccine is prepared by coating recombinant Bacillus subtilis spore CyHV-2 having a surface showing a CyHV-2 immune protective antigen with a Carassius auratus gibelio granular feed; and the above spore is a spore with germination defects and no antibiotic-resistant gene markers. The preparation method comprises the following steps: constructing the recombinant Bacillus subtilis spore CyHV-2 having germination defects, having no antibiotic resistance gene markers and having a surface showing the CyHV-2 immune protective antigen, and preparing the Carassius auratus gibelio granular feed coated CyHV-2 oral recombinant Bacillus subtilis spore vaccine used for preventing and treating CyHV-2 induced Carassius auratus gibelio hemorrhage. The oral recombinant Bacillus subtilis spore vaccine having germination defects, having no antibiotic resistance gene markers and having a surface showing the CyHV-2 immune protective antigen, prepared in the invention, has the characteristics of safety and high efficiency.

The invention discloses a primary culture and purification method of intestinal epithelial cells of carassius auratus gibelio. The method comprises the following steps of: sampling intestinal epithelial tissues, preparing a culture solution, culturing tissue blocks and purifying the intestinal epithelial tissues. According to the method disclosed by the invention, processes of separating, culturing and purifying the intestinal epithelial cells of the carassius auratus gibelio, and ingredients of the culture solution, are improved. According to the primary culture method of the intestinal epithelial cells of the carassius auratus gibelio, the time for culturing to normal cohesion of the cells can be shortened to 4-8 hours, the culture time is greatly shortened, and a constructed intestinal epithelial cell model of carassius auratus gibelio can be used for carrying out related researches on nutrient digestion and absorption mechanisms, the cell toxicity of foreign substances and the development and metabolism of the cells.

The invention relates to a puffing feed for carassius auratus gibelio and a preparation method of the puffing feed, and belongs to the technical field of feeds for aquatic products. The puffing feed comprises the following components (in parts by weight): 8-12 parts of fish meal, 3-5 parts of 46% soybean meal, 15-20 parts of 46% cottonseed meal, 5-10 parts of rapeseed meal, 5-10 parts of fermented rapeseed meal, 8-15 parts of wheat, 5-10 parts of rice bran, 5-10 parts of a vinasse protein feed, 5-10 parts of meat powder, 2-3.5 parts of soybean oil, 1.5-2 parts of bentonite, 1.5-3 parts of calcium dihydrogen phosphate, 0.3-0.5 part of table salt and 1.0-2.5 parts of a premix. The puffing feed is prepared through the following steps of concocting the raw materials, transferring the concocted materials into an extruding, puffing and granulating machine so as to obtain granular puffing feeds, drying the puffed materials, spraying the soybean oil, cooling the puffed materials sprayed with the soybean oil, grading the cooled puffed feeds, weighing finished products, and loading the weighed finished products into bags. The puffed feed for carassius auratus gibelio, disclosed by the invention, is rich in nutrition, stable in water and high in utilization rate, the water pollution is reduced, the growth speed of the carassius auratus gibelio is high, and the disease resistance is high.

The invention discloses a method suitable for cultivating carassius auratus gibelio 'Zhongke No. 3' early spring fries. The method comprises the following steps of mounting a micropore aerator pipe atthe bottom of a fry cultivation pond, and performing standard coarse cultivation on the carassius auratus gibelio 'Zhongke No. 3' early spring fries in three stages by taking particle fermented fishpaste and fry puffed micro-particle compound feed as main baits. Compared with a traditional fertilizer water and soybean milk fry cultivation method, the cultivation method provided by the inventioncan solve the problems that pond plankton bait is difficult to cultivate, large-scale supply cannot be guaranteed, the fry cultivation survival rate is low, and the cultivation density is limited in early spring low-temperature rainy weather, and the unit yield and survival rate in the carassius auratus gibelio 'Zhongke No. 3' early spring fry cultivation are effectively increased; and the growth,the stress resistance and the fry quality of early spring fries are obviously improved.

The invention provides an artificial carassius auratus breeding method, and relates to the field of aquaculture. The artificial carassius auratus breeding method comprises the following steps that pond cleaning and disinfection treatment are carried out on a fish pond before fries are put into the pond, water is injected into the fish pond, the fires are put in the pond one week after water injection, shrimps and snails are put in the fish pond; the fries are fed with a mixed feed with the protein content ranging from 39% to 40%, 10-20 kg of quicklime is put in the fish pond per mu according to the water quality conditions to regulate water quality, and the fish pond is replenished with fresh water at fixed periods. According to the artificial carassius auratus breeding method, additives are added into the mixed feed and achieve the effects of feeding promotion and growth promotion, source of raw materials of the additives is stable, the smell and taste requirements of carassius auratus for the mixed feed can be met, and the ingestion speed and feed intake are increased remarkably; the effective constituents and ratio of the additives of the feed are optimized by the inventor through long-time experiments, the feed promotion effect can be enhanced, the anti-stress capacity and the growth rate of carassius auratus can be remarkably increased, and the feed coefficient is reduced; the additives of the feed are safe, efficient and free of toxic and side effects in long-term use.

The present invention relates to compound feed for adult fish of carassius auratus, belonging to the technical field of aquaculture. The compound feed comprises the following ingredients by weight: 7-14% of domestic fish meal, 6-9% of corn gluten meal, 22-28% of soybean meal, 12-16% of cottonseed meal, 10-13% of rapeseed meal, 7-9% of rice bran, 5.83-9.63% of wheat middling, 4-6% of Saccharomyces cerevisiae, 4.5-5.3% of soybean oil, 0.5-1.0% of soy lecithin, 0.7-1.5% of calcium dihydrogen phosphate, 0.05-0.1% of L-ascorbate-2-monophosphate, 3-4% of zeolite powder, 0.2-0.3% of choline chloride, 0.2-0.4% of salt, 0.01-0.03% of ethoxyquin, 0.5-1.0% of mineral premix, and 0.3-0.8% of vitamin premix. According to the present invention, habits, nutritional needs and digestion characteristics of carassius auratus are fully taken into account, and the compound feed is reasonable in combination by selecting raw materials easily digested and absorbed by carassius auratus, and has good palatability, a high feed conversion ratio, little pollution to water bodies, stable sources of raw materials, and low cost, suitable for large-scale adult fish farming of carassius auratus.

The invention discloses a carassius auratus feed, and raw materials of the feed comprise in parts by weight: 15-25 parts of high gluten flour, 20-40 parts of corn starch; 5-15 parts of linseed meal, 5-10 parts of acetes, 12-18 parts of gulfweed, 2-8 parts of puffed sweet potato powder, 15-24 parts of soybean meal, 2-12 parts of rapeseed meal, 5-10 parts of wheat bran, 1-2 parts of fish oil, 1-2 parts of corn oil, 0.5-1.5 parts of betaine, 0.2-0.6 part of EM microbial solution, 1-2 parts of soybean phospholipid, and 1-4 parts of premix. The raw materials of the premix comprise, in parts by weight, 5-15 parts of corn starch, 0.5-1.5 parts of gelatin multivitamin E, 1-2 parts of vitamins, 1-2 parts of mineral salt, 0.5-1 part of clam extract, 1-2 parts of bentonite, and 1-2 parts of grape seed oil.

The invention relates to a carassius auratus gibelio feed for preventing and treating cyprinid herpesvirus-2. The feed is mainly composed of the following raw materials: fish meal, puffed soybeans, soybean meal, rapeseed meal, cottonseed meal, fine rice bran, flour, soybean oil, monocalcium phosphate, zeolite powder, bentonite, a vitamin premix and a Chinese herbal medicine feed additive. The feedof the invention can effectively reduce the occurrence of diseases in breeding processes, particularly the occurrence of fulminating bleeding diseases, and can improve the survival rate of carassiusauratus gibelio, and promote the growth of the carassius auratus gibelio.

The invention discloses an egg yolk antibody microcapsule feed additive for resisting carassius auratus gibelio bacterial septicemia. The additive consists of three kinds of raw materials, namely, egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia, astragalus powder and beta-cyclodextrin, wherein the egg yolk antibody freeze-dried powder for resisting the carassius auratus gibelio bacterial septicemia is mainly used for lowering the infection rate of carassius auratus gibelio by three kinds of mesophilic aeromonas or preventing carassius auratus gibelio from being infected by the three kinds of mesophilic aeromonas; the astragalus powder is used for enhancing the immunity of the carassius auratus gibelio; the beta-cyclodextrin is mainly used for realizing embedding and slow release of the egg yolk antibody freeze-dried powder. When the microcapsule feed additive and a commercially available carassius auratus gibelio compound feed are fed together according to a certain proportion, diseases caused by three kinds of pathogenic mesophilic aeromonas can be prevented and treated during cultivation of the carassius auratus gibelio, the yield of the carassius auratus gibelio is increased, and the economical benefit of carassius auratus gibelio cultivation is increased remarkably. The problem of bacterial septicemia in current carassius auratus gibelio production is solved.

The invention relates to a mixed breeding method of carassius auratus gibelio and penaeus vannamei boone. The method comprises the steps of pond preparation, fingerling and young shrimp putting, bait feeding, breeding management and capturing. The mixed breeding method of the carassius auratus gibelio and the penaeus vannamei boone is simple, the breeding cost is low, the breeding yield can be greatly increased, the total revenue of breeding of the carassius auratus gibelio and the penaeus vannamei boone is improved, and a good application prospect is achieved.

The present invention discloses a preparation method of nourishing carassius auratus soup. The preparation method comprises the following steps: scales, gills and internal organs of carassius auratus are removed, the treated carassius auratusis washed clean, and the washed carassius auratus body is cut into several sections for standby applications; the prepared carassius auratus is pickled using cooking wine and soy sauce for 20-30 minutes, and then the used cooking wine and soy sauce for the pickling are removed; the pickled carassius auratus is pickled with fresh gingers, white sugar, edible salt and a little mutton soup for more than 30 minutes; oil is cooked to be well-done using a pot, star anise, cassia barks and Chinese prickly ash are put into the pot and stir-fried to be almost well-done, the mutton soup and tofu blocks are added, and then the pickled carassius auratus and the mutton soup for the pickling are added; big fire is used to boil out the mutton soup, the mixture is slowly simmered for 5-10 minutes using moderate fire, bay leaves, red jujubes, Chinese wolfberry fruits, lotus seeds, Chinese yams, white fungus, and papayas are added, and the mixture is simmered for 15-30 minutes; and minced garlic, chopped green onions, coriander, vinegar and monosodium glutamate are successively added, the mixture is boiled out, the fired is turned off, and the nourishing carassius auratus soup is obtained. The preparation method is simple, easy to operate and easy to get raw materials. The nourishing carassius auratus soup is more delicious in taste, and has effects of benefiting stomach and promoting salivation, nourishing yin and supplementing yang, and nourishing skin and supplementing blood.

The invention relates to a special Chinese herbal medicine feed additive for carassius auratus gibelio, which is prepared by the following raw materials in parts by weight: 7-9 parts of pteris multifida poir, 3-5 parts of oriental wormwood, 2-4 parts of fructus forsythiae, 8-10 parts of garlic, 1-2 parts of salvia miltiorrhiza, 3-5 parts of myrobalan, 3-5 parts of folium isatidis, 2-4 parts of astragalus mongholicus and 10-20 parts of carrier. With the adoption of the special Chinese herbal medicine feed additive for the carassius auratus gibelio, economic benefits of carassius auratus gibelio culture can be improved obviously; the Chinese herbal medicine feed additive can significantly reduce the disease incidence during the carassius auratus gibelio culture, particularly the incidence of an explosive hemorrhagic disease; a survival rate is increased by 9-12%; growth of the carassius auratus gibelio is promoted; the quality of the carassius auratus gibelio can be improved; and the cost of the raw materials used in the Chinese herbal medicine feed additive, the production cost and the use cost are lower, so that cost saving is facilitated.

The invention discloses a compound probiotic preparation for preventing and treating gill hemorrhagic disease of carassius auratus gibelio and a preparation method thereof. The preparation consists of12-16 parts of rapeseed meal, 10-14 parts of fish meal, 2-6 parts of soybean oil, 3-7 parts of corn flour, 1-3 parts of a premix, 1-3 parts of monocalcium phosphate, 6-8 parts of microcrystalline cellulose, 15-19 parts of wheat flour, 2-6 parts of fish oil, 26-30 parts of soybean meal and 0.07-0.12 part of bacillus. The preparation can improve the disease resistance of the carassius auratus gibelio which is a high-quality breeding variety to CyHV-2 viruses, and enhance the immunity of the carassius auratus gibelio from the perspectives of probiotics and immunopotentiators so as to improve thedisease resistance of the carassius auratus gibelio.

The present invention discloses a carassius auratus and tofu mixed mushroom soup and a preparation method thereof. The carassius auratus and tofu mixed mushroom soup consists of main and auxiliary materials. The main materials includes the following components in parts by weight: white beech mushrooms 5-7 parts, agrocybe aegirit 4-6 parts, hypsizygus marmoreus 5-8 parts, tofu 6-9 parts, shrimps 1-3 parts and carassius auratus 3-7 parts. The auxiliary materials includes the following components in parts by weight: fresh ginger 1-3 parts, carrots 2-4 parts, Chinese yams 3-5 parts, hemerocallis citrina 1-4 parts, chopped green onion 1-3 parts, flour 3-5 parts, high-grade Shaoxing wine 2-5 parts, olive oil 1-3 parts and chicken essence 1-3 parts. The carassius auratus and tofu mixed mushroom soup is rich in minerals and vitamins, and has efficacies of strengthening middle warmer and tonifying qi, clearing heat and moistening dryness, promoting salivation and quenching thirst, and cleaning intestines and stomach.