The invention discloses a three-dimensional culture method for soft shell crayfishes in indoor circulating water and belongs to the field of aquatic product culture. Culture equipment and a culture method are provided. The culture equipment comprises a culture box, a goods shelf, a biofilter, a water storage pond, a circulating water pump, an aquaculture thermostat, an aerator, a light supplement lamp and a monitoring camera; and the culture method comprises the steps: preparation before stocking, fry stocking and daily management. The indoor culture is not affected by seasons and weathers, so that the soft shell crayfishes can be supplied all the year round; the culture box can realize microporous aeration by a bottom interlayer to supply oxygen to be saturated, so that not only is the demand of high-speed growth of the crayfishes met, but also the problem that the crayfishes get ill is solved; the aquaculture thermostat is used for setting an optimal temperature for the growth of the crayfishes, so that the crayfishes grow rapidly; the crayfishes live in a single room, so that the phenomenon that the crayfishes kill each other can be avoided, and the culture density and the molting rate are increased; due to the adoption of the monitoring camera, the labor intensity of working personnel can be reduced, and high-quality soft shell crayfish products can be obtained; and the method can also be used for crayfish culture.