The invention discloses a RAA amplification primer and a probe for
rapid detection of
Cyprinid herpesvirus II, a detection kit and an application method thereof. The RAA isothermal amplification
system of the invention has fast reaction and wide temperature range, can realize effective amplification of target genes at 34-40 DEG C, has a lowest
detection limit of 8.14*100 copies / microliter, has a higher sensitivity than common PCR of CyHV-2, and will not cross-react with other
aquatic animal epidemics. The kit of the invention can quickly, efficiently and sensitively detect
Cyprinid herpesvirus II, is simple to operate, has high specificity, is easy to observe reaction results, and is safe and
pollution-free. The invention not only provides an effective technical means for on-site
rapid detection and screening of
Cyprinid herpesvirus type II infection
nucleic acid, but also has very important significance for controlling the infection transmission of the
virus in ornamental fish such as
crucian carp and goldfish and the inspection and
quarantine in epidemic areas and entry and exit ports.