Application of ferrous ions in the prevention and treatment of carp herpes virus type Ⅱ (cyhv2) infection

A carp herpes virus, prevention and treatment technology, applied in the field of medical use of ferrous ions, can solve the problems of limited prevention and control methods, and researchers have not yet found a prevention and treatment method

Active Publication Date: 2022-05-03
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment methods for viral diseases in aquatic animals are very limited. Although some studies have been carried out on the isolation of fish herpes virus pathogens, histopathological observation, and transcriptome analysis, researchers have not yet found a good prevention method

Method used

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  • Application of ferrous ions in the prevention and treatment of carp herpes virus type Ⅱ (cyhv2) infection
  • Application of ferrous ions in the prevention and treatment of carp herpes virus type Ⅱ (cyhv2) infection
  • Application of ferrous ions in the prevention and treatment of carp herpes virus type Ⅱ (cyhv2) infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: FeCl 2 Effects on the lethality of crucian carp infected with CyHV2

[0013] It is carried out in an aquarium of 35cm×23.5cm×36cm, and the water is changed every day and the water temperature is kept at about 22°C. About 5g of healthy crucian carp was injected intraperitoneally with CyHV2 virus suspension to establish an artificial infection model. The control group was intraperitoneally injected with a mixture of 250 μl / tail of virus suspension and 50 μl / tail of PBS, and the experimental group used 250 μl / tail of virus suspension according to the total volume of 300 μl / tail. Tail diluted FeCl 2 solution (0.12675g / ml), manganese chloride solution (MnCl 2 , 0.19791g / ml), copper chloride solution (CuCl 2 , 0.17048g / ml), magnesium chloride solution (MgCl 2 , 0.09521g / ml), calcium chloride solution (CaCl 2 , 0.111g / ml) to a final concentration of 0.1mg / g (fill up the volume with PBS), and inject infection at the same time. Record crucian carp death situatio...

Embodiment 2

[0014] Example 2: FeCl 2 Effects on the expression level of viral genes in crucian carp infected with CyHV2

[0015] Detect fish infected with CyHV2 with 0.1mg / g FeCl 2 After treatment, the expression of virus-related genes in fish. Process control group and FeCl respectively according to the method described in embodiment 1 2 -CyHV2 mixed solution group, each group of crucian carp was intraperitoneally injected with 300μl mixed solution, anesthetized with MS-222 (0.1mg / ml) 6 days after infection, and dissected the spleen (Spleen), head kidney (Headkidney) and body kidney (Kidney) samples , total tissue RNA was extracted using TRIzol (Invitrogen), and cDNA was obtained by reverse transcription with GoScript Reverse Transcription Kit (Promega). The expression of virus-related genes was detected by qPCR ( figure 2 ) (5 biological repetitions were set for each group), the results showed that: 0.1mg / g FeCl 2 After treatment, the expression of virus-related genes (including O...

Embodiment 3

[0016] Example 3: FeCl 2 Effects on the expression of genes related to antiviral immunity in crucian carp infected with CyHV2

[0017] Further detection of fish infected with CyHV2 with 0.1mg / g FeCl 2 After treatment, the expression of antiviral immune genes. About 5g of healthy crucian carp was divided into PBS group, PBS-FeCl 2 Mixed solution group, FeCl 2 -CyHV2 mixed solution group and CyHV2 virus suspension group. Among them, the PBS group was injected with 300 μl / tail of PBS; PBS-FeCl 2 The mixed solution group used PBS to dilute FeCl 2 Solution to 0.1mg / g, the total volume is 300μl / tail; FeCl 2 -CyHV2 mixed solution group uses CyHV2 virus suspension to dilute FeCl 2 Solution to 0.1mg / g; CyHV2 virus suspension group CyHV2 virus suspension and FeCl 2 The same volume of PBS was mixed with the solution; the infection was injected at the same time as described above, and after 6 days of infection, anesthesia was dissected, and the spleen, head kidney and body kidney ...

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Abstract

The invention discloses the application of ferrous ions in the prevention and treatment of cyprinid herpesvirus 2 (CyHV2) infection. The establishment of experimental animal infection models shows that ferrous chloride can significantly protect crucian carp from CyHV2 infection. Combined with the results of qPCR experiments, it was found that the transcription level of virus-related genes was significantly inhibited after ferrous chloride treatment. Therefore, the compound containing ferrous ions can be used as a drug for preventing and treating CyHV2, and has the advantages of non-toxic and safe dosage and good protective effect.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the medical use of ferrous ions. The compound containing ferrous ions can be used to prevent and treat aquatic animal diseases caused by Cypies herpesvirus type II (CyHV2) infection. Background technique [0002] Iron is one of the six important trace elements in organisms, and is an essential element for the normal growth and life of animals and plants. In living organisms, iron undertakes various biological functions. Generally, iron is present in the body as Fe 2+ / 3+ Stored in the form of heme, the function of +2 ferrous ions is manifested in many ways. [0003] As an important component of hemoglobin, ferrous ions are responsible for carrying oxygen and transporting oxygen to realize the oxygen transport function of red blood cells. Myoglobin, which is used for muscle oxygen storage, contains a heme iron, which can provide or supplement the insufficient blo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/26A61P31/22
CPCA61K33/26A61P31/22
Inventor 李顺李卓聪
Owner INST OF AQUATIC LIFE ACAD SINICA
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