CyHV-2 (Cyprinid Herpesvirus 2) monoclonal antibody, hybridoma cell and application of CyHV-2 monoclonal antibody
A monoclonal antibody, hybridoma cell technology, applied in antiviral immunoglobulin, biochemical equipment and methods, instruments, etc., can solve the problems of inconvenient rapid diagnosis, lag, single detection method, etc., to improve the detection speed and detection. Efficiency, good specificity and potency, highly specific effects
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[0021] 1. Materials and methods
[0022] 1. Viral Antigens and Reagents
[0023] CyHV-2 antigen: from CyHV-2-infected diseased fish tissue, tissue grinding samples were taken, and purified by sucrose gradient centrifugation. Escherichia coli TG1 was provided by Dai Heping, a researcher at the Institute of Hydrobiology, Chinese Academy of Sciences.
[0024] 2YT medium: Weigh 17g tryptone, 10g yeast extract, 5g NaCl, dissolve in double distilled water, dilute to 1L, and sterilize by high pressure steam. Prepare solid medium, add 15g agar powder, and sterilize with high pressure steam.
[0025] SOBAG medium: Weigh 20g tryptone, 5g yeast extract, 0.5g NaCl, dissolve in double distilled water, and dilute to 1L. With solid medium, add 15g agar powder. Autoclaved. When it is cooled to 40-50°C, add 10 mL of 1M MgCl2 that has been sterilized, so that the Amp is 100 μg / mL and the glucose content is 2%.
[0026] 2. CyHV-2 monoclonal antibody preparation
[0027] 2.1 Immunity
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